In Vitro Antithrombotic, Antitumor and Antiangiogenic Activities of Green Tea Polyphenols and Its Main Constituent Epigallocatechin-3-gallate
The balance between embolic risk and bleeding represents a clinical challenge in cancer patient treatment, encouraging studies on adjuvant oncologic treatments. Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation...
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| Veröffentlicht in: | Processes 2023-01, Vol.11 (1), p.76 |
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| creator | Luz, Jefferson Romáryo Duarte da López, Jorge A. Ferreira, Macelia Pinheiro de Sousa, Rubiamara Mauricio Silva, Saulo Victor e Almeida, Maria das Graças Araujo-Silva, Gabriel |
| description | The balance between embolic risk and bleeding represents a clinical challenge in cancer patient treatment, encouraging studies on adjuvant oncologic treatments. Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation and the antineoplastic effect on melanoma cells (B16-F10) by applying platelet aggregation, angiogenesis and viability cell assays. The results displayed a significant platelet antiaggregant effect, corresponding to 50 and 80% for the extract and EGCG, respectively, compared to the negative control. Furthermore, both GTE and EGCG exhibited antitumor effects by reducing melanoma cell growth by 25 and 50%, respectively, verified by cellular apoptosis. Regarding angiogenesis, these substances inhibited blood vessel formation, reaching about 25% and 99% for GTE and EGCG at 100 μg/mL, respectively. Moreover, TNF-α cell stimulation evidenced VEGF and IL-8 secretion inhibition at 55 and 20% with GTE, while EGCG promoted an inhibition around 78% for both VEGF and IL-8. The results indicate the promising performance of GTE and EGCG as an option for treating cancer and its side effects. Nonetheless, further studies are required to elucidate their action mechanism on clotting, cell death and angiogenesis. |
| doi_str_mv | 10.3390/pr11010076 |
| format | Article |
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Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation and the antineoplastic effect on melanoma cells (B16-F10) by applying platelet aggregation, angiogenesis and viability cell assays. The results displayed a significant platelet antiaggregant effect, corresponding to 50 and 80% for the extract and EGCG, respectively, compared to the negative control. Furthermore, both GTE and EGCG exhibited antitumor effects by reducing melanoma cell growth by 25 and 50%, respectively, verified by cellular apoptosis. Regarding angiogenesis, these substances inhibited blood vessel formation, reaching about 25% and 99% for GTE and EGCG at 100 μg/mL, respectively. Moreover, TNF-α cell stimulation evidenced VEGF and IL-8 secretion inhibition at 55 and 20% with GTE, while EGCG promoted an inhibition around 78% for both VEGF and IL-8. The results indicate the promising performance of GTE and EGCG as an option for treating cancer and its side effects. Nonetheless, further studies are required to elucidate their action mechanism on clotting, cell death and angiogenesis.</description><identifier>ISSN: 2227-9717</identifier><identifier>EISSN: 2227-9717</identifier><identifier>DOI: 10.3390/pr11010076</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Angiogenesis ; Antiangiogenics ; Anticancer properties ; Anticoagulants ; Antitumor activity ; Apoptosis ; Blood vessels ; Cancer ; Cancer therapies ; Cell death ; Chemotherapy ; Clotting ; Drugs ; Epigallocatechin-3-gallate ; Green tea ; Hemostasis ; Hemostatics ; Interleukin 8 ; Melanoma ; Metabolites ; Mortality ; Natural products ; Penicillin ; Physiology ; Plasma ; Platelet aggregation ; Platelets ; Polyphenols ; Radiation therapy ; Side effects ; Skin cancer ; Tea ; Tumor necrosis factor-α ; Vascular endothelial growth factor</subject><ispartof>Processes, 2023-01, Vol.11 (1), p.76</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. 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Thereby, this study evaluated the in vitro effect of green tea extract (GTE) and epigallocatechin-3-gallate (EGCG) on hemostasis modulation and the antineoplastic effect on melanoma cells (B16-F10) by applying platelet aggregation, angiogenesis and viability cell assays. The results displayed a significant platelet antiaggregant effect, corresponding to 50 and 80% for the extract and EGCG, respectively, compared to the negative control. Furthermore, both GTE and EGCG exhibited antitumor effects by reducing melanoma cell growth by 25 and 50%, respectively, verified by cellular apoptosis. Regarding angiogenesis, these substances inhibited blood vessel formation, reaching about 25% and 99% for GTE and EGCG at 100 μg/mL, respectively. Moreover, TNF-α cell stimulation evidenced VEGF and IL-8 secretion inhibition at 55 and 20% with GTE, while EGCG promoted an inhibition around 78% for both VEGF and IL-8. 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| subjects | Angiogenesis Antiangiogenics Anticancer properties Anticoagulants Antitumor activity Apoptosis Blood vessels Cancer Cancer therapies Cell death Chemotherapy Clotting Drugs Epigallocatechin-3-gallate Green tea Hemostasis Hemostatics Interleukin 8 Melanoma Metabolites Mortality Natural products Penicillin Physiology Plasma Platelet aggregation Platelets Polyphenols Radiation therapy Side effects Skin cancer Tea Tumor necrosis factor-α Vascular endothelial growth factor |
| title | In Vitro Antithrombotic, Antitumor and Antiangiogenic Activities of Green Tea Polyphenols and Its Main Constituent Epigallocatechin-3-gallate |
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