PHYTOCHEMICAL CONSTITUENTS AND ANTILEUKEMIC EFFECTS OF JUNIPERUS OXYCEDRUS EXTRACT
Aim: Many genetic and environmental factors can be effective in the process of cancerization. Preventing the progression of leukemia may be possible by controlling the pathways involving mechanisms such as apoptosis and autophagy. When the literature is examined, there are studies showing the effect...
Gespeichert in:
Veröffentlicht in: | Biotechnologia acta 2022-10, Vol.15 (5), p.64-70 |
---|---|
1. Verfasser: | |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 70 |
---|---|
container_issue | 5 |
container_start_page | 64 |
container_title | Biotechnologia acta |
container_volume | 15 |
creator | TURAK, Berfin Tugba |
description | Aim: Many genetic and environmental factors can be effective in the process of cancerization. Preventing the progression of leukemia may be possible by controlling the pathways involving mechanisms such as apoptosis and autophagy. When the literature is examined, there are studies showing the effects of various types of juniper on various cancer cell lines, including human chronic myeloid leukemia cells, but the signal pathways in which they act are not fully known. In this study, the anticancer effects of Juniperus oxycedrus extract on K-562 human chronic myeloid leukemia cells were investigated. Method: After the cells were treated with the Juniperus oxycedrus extract, cytotoxicity and gene expression analyzes were performed. Changes in the expression of Akt, the member of the PI3K/Akt/mTOR signaling pathway; caspase 3, which is one of the main effective genes in the pathways regulating apoptosis; and the apoptosis suppressor BCL-2 gene, which is an oncogene, were investigated. Results: According to the MTT test results, Juniperus oxycedrus extract showed over approximately 50% cell viability in K-562 cells at all doses. The most appropriate dose of Juniperus oxycedrus fruit extract in this research was determined as 50 µg/ mL considering cell viability. After the gene expression analysis, it was observed that Akt expression increased 1.092 times, BCL-2 expression decreased approximately 0.3 times, and caspase 3 expression increased 1.2 times. Conclusions: Constituents of Juniperus oxycedrus plant may have apoptotic effects on chronic myeloid leukemia cells. |
doi_str_mv | 10.15407/biotech15.05.064 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2763572688</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2763572688</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1614-f4d9199eef9db905b825177325669b6d7fbb2804429140ec8109e079257044d23</originalsourceid><addsrcrecordid>eNo9UE1PwkAQ3RhNJMgP8NbEc3F2ux_dI1m2UsWWwDaB04a22whRiy0c_PcuakjeZN7Me5mXDEL3GMaYURCP5a49uuoNszF4cHqFBoRiCIXg6-sLZ_gWjfp-DwAYIsooGaDlYrYxuZrp11RN5oHKs5VJTaEzswom2dSXSee6eDnrgU4SrbyQJ8FzkaULvSz8sN4oPT0zvTbLiTJ36KbZvvdu9N-HqEi0UbNwnj-dQ8IKc0zDhtYSS-lcI-tSAitjwrAQEWGcy5LXoilLEgOlRGIKrooxSAdCEib8sibRED383T107dfJ9Ue7b0_dp4-0RPCICcLj2Lvwn6vq2r7vXGMP3e5j231bDPb3e_byPQsenEY_64ZbRQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2763572688</pqid></control><display><type>article</type><title>PHYTOCHEMICAL CONSTITUENTS AND ANTILEUKEMIC EFFECTS OF JUNIPERUS OXYCEDRUS EXTRACT</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>TURAK, Berfin Tugba</creator><creatorcontrib>TURAK, Berfin Tugba ; Department of Bioengineering, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey</creatorcontrib><description>Aim: Many genetic and environmental factors can be effective in the process of cancerization. Preventing the progression of leukemia may be possible by controlling the pathways involving mechanisms such as apoptosis and autophagy. When the literature is examined, there are studies showing the effects of various types of juniper on various cancer cell lines, including human chronic myeloid leukemia cells, but the signal pathways in which they act are not fully known. In this study, the anticancer effects of Juniperus oxycedrus extract on K-562 human chronic myeloid leukemia cells were investigated. Method: After the cells were treated with the Juniperus oxycedrus extract, cytotoxicity and gene expression analyzes were performed. Changes in the expression of Akt, the member of the PI3K/Akt/mTOR signaling pathway; caspase 3, which is one of the main effective genes in the pathways regulating apoptosis; and the apoptosis suppressor BCL-2 gene, which is an oncogene, were investigated. Results: According to the MTT test results, Juniperus oxycedrus extract showed over approximately 50% cell viability in K-562 cells at all doses. The most appropriate dose of Juniperus oxycedrus fruit extract in this research was determined as 50 µg/ mL considering cell viability. After the gene expression analysis, it was observed that Akt expression increased 1.092 times, BCL-2 expression decreased approximately 0.3 times, and caspase 3 expression increased 1.2 times. Conclusions: Constituents of Juniperus oxycedrus plant may have apoptotic effects on chronic myeloid leukemia cells.</description><identifier>ISSN: 2410-7751</identifier><identifier>ISSN: 1995-5537</identifier><identifier>EISSN: 2410-776X</identifier><identifier>DOI: 10.15407/biotech15.05.064</identifier><language>eng</language><publisher>Kyiv: Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Anticancer properties ; Apoptosis ; Autophagy ; Bcl-2 protein ; Caspase-3 ; Cell viability ; Chronic myeloid leukemia ; Constituents ; Cytotoxicity ; Environmental factors ; Gene expression ; Juniperus oxycedrus ; Leukemia ; Myeloid leukemia ; Signal transduction ; Statistical analysis ; TOR protein ; Toxicity ; Tumor cell lines</subject><ispartof>Biotechnologia acta, 2022-10, Vol.15 (5), p.64-70</ispartof><rights>Copyright Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1614-f4d9199eef9db905b825177325669b6d7fbb2804429140ec8109e079257044d23</citedby><cites>FETCH-LOGICAL-c1614-f4d9199eef9db905b825177325669b6d7fbb2804429140ec8109e079257044d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,27905,27906</link.rule.ids></links><search><creatorcontrib>TURAK, Berfin Tugba</creatorcontrib><creatorcontrib>Department of Bioengineering, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey</creatorcontrib><title>PHYTOCHEMICAL CONSTITUENTS AND ANTILEUKEMIC EFFECTS OF JUNIPERUS OXYCEDRUS EXTRACT</title><title>Biotechnologia acta</title><description>Aim: Many genetic and environmental factors can be effective in the process of cancerization. Preventing the progression of leukemia may be possible by controlling the pathways involving mechanisms such as apoptosis and autophagy. When the literature is examined, there are studies showing the effects of various types of juniper on various cancer cell lines, including human chronic myeloid leukemia cells, but the signal pathways in which they act are not fully known. In this study, the anticancer effects of Juniperus oxycedrus extract on K-562 human chronic myeloid leukemia cells were investigated. Method: After the cells were treated with the Juniperus oxycedrus extract, cytotoxicity and gene expression analyzes were performed. Changes in the expression of Akt, the member of the PI3K/Akt/mTOR signaling pathway; caspase 3, which is one of the main effective genes in the pathways regulating apoptosis; and the apoptosis suppressor BCL-2 gene, which is an oncogene, were investigated. Results: According to the MTT test results, Juniperus oxycedrus extract showed over approximately 50% cell viability in K-562 cells at all doses. The most appropriate dose of Juniperus oxycedrus fruit extract in this research was determined as 50 µg/ mL considering cell viability. After the gene expression analysis, it was observed that Akt expression increased 1.092 times, BCL-2 expression decreased approximately 0.3 times, and caspase 3 expression increased 1.2 times. Conclusions: Constituents of Juniperus oxycedrus plant may have apoptotic effects on chronic myeloid leukemia cells.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Autophagy</subject><subject>Bcl-2 protein</subject><subject>Caspase-3</subject><subject>Cell viability</subject><subject>Chronic myeloid leukemia</subject><subject>Constituents</subject><subject>Cytotoxicity</subject><subject>Environmental factors</subject><subject>Gene expression</subject><subject>Juniperus oxycedrus</subject><subject>Leukemia</subject><subject>Myeloid leukemia</subject><subject>Signal transduction</subject><subject>Statistical analysis</subject><subject>TOR protein</subject><subject>Toxicity</subject><subject>Tumor cell lines</subject><issn>2410-7751</issn><issn>1995-5537</issn><issn>2410-776X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNo9UE1PwkAQ3RhNJMgP8NbEc3F2ux_dI1m2UsWWwDaB04a22whRiy0c_PcuakjeZN7Me5mXDEL3GMaYURCP5a49uuoNszF4cHqFBoRiCIXg6-sLZ_gWjfp-DwAYIsooGaDlYrYxuZrp11RN5oHKs5VJTaEzswom2dSXSee6eDnrgU4SrbyQJ8FzkaULvSz8sN4oPT0zvTbLiTJ36KbZvvdu9N-HqEi0UbNwnj-dQ8IKc0zDhtYSS-lcI-tSAitjwrAQEWGcy5LXoilLEgOlRGIKrooxSAdCEib8sibRED383T107dfJ9Ue7b0_dp4-0RPCICcLj2Lvwn6vq2r7vXGMP3e5j231bDPb3e_byPQsenEY_64ZbRQ</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>TURAK, Berfin Tugba</creator><general>Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BYOGL</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>LK8</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope></search><sort><creationdate>202210</creationdate><title>PHYTOCHEMICAL CONSTITUENTS AND ANTILEUKEMIC EFFECTS OF JUNIPERUS OXYCEDRUS EXTRACT</title><author>TURAK, Berfin Tugba</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1614-f4d9199eef9db905b825177325669b6d7fbb2804429140ec8109e079257044d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>1-Phosphatidylinositol 3-kinase</topic><topic>AKT protein</topic><topic>Anticancer properties</topic><topic>Apoptosis</topic><topic>Autophagy</topic><topic>Bcl-2 protein</topic><topic>Caspase-3</topic><topic>Cell viability</topic><topic>Chronic myeloid leukemia</topic><topic>Constituents</topic><topic>Cytotoxicity</topic><topic>Environmental factors</topic><topic>Gene expression</topic><topic>Juniperus oxycedrus</topic><topic>Leukemia</topic><topic>Myeloid leukemia</topic><topic>Signal transduction</topic><topic>Statistical analysis</topic><topic>TOR protein</topic><topic>Toxicity</topic><topic>Tumor cell lines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TURAK, Berfin Tugba</creatorcontrib><creatorcontrib>Department of Bioengineering, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>East Europe, Central Europe Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><jtitle>Biotechnologia acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TURAK, Berfin Tugba</au><aucorp>Department of Bioengineering, Faculty of Engineering and Natural Sciences, Üsküdar University, Istanbul, Turkey</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PHYTOCHEMICAL CONSTITUENTS AND ANTILEUKEMIC EFFECTS OF JUNIPERUS OXYCEDRUS EXTRACT</atitle><jtitle>Biotechnologia acta</jtitle><date>2022-10</date><risdate>2022</risdate><volume>15</volume><issue>5</issue><spage>64</spage><epage>70</epage><pages>64-70</pages><issn>2410-7751</issn><issn>1995-5537</issn><eissn>2410-776X</eissn><abstract>Aim: Many genetic and environmental factors can be effective in the process of cancerization. Preventing the progression of leukemia may be possible by controlling the pathways involving mechanisms such as apoptosis and autophagy. When the literature is examined, there are studies showing the effects of various types of juniper on various cancer cell lines, including human chronic myeloid leukemia cells, but the signal pathways in which they act are not fully known. In this study, the anticancer effects of Juniperus oxycedrus extract on K-562 human chronic myeloid leukemia cells were investigated. Method: After the cells were treated with the Juniperus oxycedrus extract, cytotoxicity and gene expression analyzes were performed. Changes in the expression of Akt, the member of the PI3K/Akt/mTOR signaling pathway; caspase 3, which is one of the main effective genes in the pathways regulating apoptosis; and the apoptosis suppressor BCL-2 gene, which is an oncogene, were investigated. Results: According to the MTT test results, Juniperus oxycedrus extract showed over approximately 50% cell viability in K-562 cells at all doses. The most appropriate dose of Juniperus oxycedrus fruit extract in this research was determined as 50 µg/ mL considering cell viability. After the gene expression analysis, it was observed that Akt expression increased 1.092 times, BCL-2 expression decreased approximately 0.3 times, and caspase 3 expression increased 1.2 times. Conclusions: Constituents of Juniperus oxycedrus plant may have apoptotic effects on chronic myeloid leukemia cells.</abstract><cop>Kyiv</cop><pub>Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine</pub><doi>10.15407/biotech15.05.064</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 2410-7751 |
ispartof | Biotechnologia acta, 2022-10, Vol.15 (5), p.64-70 |
issn | 2410-7751 1995-5537 2410-776X |
language | eng |
recordid | cdi_proquest_journals_2763572688 |
source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
subjects | 1-Phosphatidylinositol 3-kinase AKT protein Anticancer properties Apoptosis Autophagy Bcl-2 protein Caspase-3 Cell viability Chronic myeloid leukemia Constituents Cytotoxicity Environmental factors Gene expression Juniperus oxycedrus Leukemia Myeloid leukemia Signal transduction Statistical analysis TOR protein Toxicity Tumor cell lines |
title | PHYTOCHEMICAL CONSTITUENTS AND ANTILEUKEMIC EFFECTS OF JUNIPERUS OXYCEDRUS EXTRACT |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T10%3A38%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PHYTOCHEMICAL%20CONSTITUENTS%20AND%20ANTILEUKEMIC%20EFFECTS%20OF%20JUNIPERUS%20OXYCEDRUS%20EXTRACT&rft.jtitle=Biotechnologia%20acta&rft.au=TURAK,%20Berfin%20Tugba&rft.aucorp=Department%20of%20Bioengineering,%20Faculty%20of%20Engineering%20and%20Natural%20Sciences,%20%C3%9Csk%C3%BCdar%20University,%20Istanbul,%20Turkey&rft.date=2022-10&rft.volume=15&rft.issue=5&rft.spage=64&rft.epage=70&rft.pages=64-70&rft.issn=2410-7751&rft.eissn=2410-776X&rft_id=info:doi/10.15407/biotech15.05.064&rft_dat=%3Cproquest_cross%3E2763572688%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2763572688&rft_id=info:pmid/&rfr_iscdi=true |