Molecular Structure from Models to Mastery: An Inquiry-Based Study of Human Insulin-Degrading Enzyme

Using crystal structure data, site directed mutagenesis, and real-time kinetic assays, students designed, expressed, and purified engineered mutants of human insulin-degrading enzyme (IDE). Students designed their own IDE mutants following in silico analysis, used inverse PCR to generate mutant plas...

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Veröffentlicht in:	Journal of chemical education 2022-12, Vol.99 (12), p.4094-4101
Hauptverfasser:	Araujo, Michael A., Barrere, Alexandra A., Tirado, Selena-Rae, Williams, Candace E., Strada, Monica I., Alper, Benjamin J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Crystal structure Design of experiments E coli Enzymes Enzymology Experimental design Experimental methods Inquiry method Insulin Molecular structure Mutagenesis Mutants Research design Students
Online-Zugang:	Volltext
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