Lipase‐catalyzed preparation of palmitic and stearic acid‐rich phosphatidylcholine

Saturated FA enhance the oxidative stability of phospholipids. In the present study phosphatidylcholine (PC) rich in palmitic and stearic acids was prepared using lipase‐catalyzed transesterification from PC isolated from egg and soybean lecithins. Two different lipases, namely, Novozym 435 and Lipo...

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Veröffentlicht in:Journal of the American Oil Chemists' Society 2005-10, Vol.82 (10), p.727-730
Hauptverfasser: Reddy, J. R. C., Vijeeta, T., Karuna, M. S. L., Rao, B. V. S. K., Prasad, R. B. N.
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container_issue 10
container_start_page 727
container_title Journal of the American Oil Chemists' Society
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creator Reddy, J. R. C.
Vijeeta, T.
Karuna, M. S. L.
Rao, B. V. S. K.
Prasad, R. B. N.
description Saturated FA enhance the oxidative stability of phospholipids. In the present study phosphatidylcholine (PC) rich in palmitic and stearic acids was prepared using lipase‐catalyzed transesterification from PC isolated from egg and soybean lecithins. Two different lipases, namely, Novozym 435 and Lipozyme TL IM, were used for the transesterification. The reaction conditions were optimized by varying the lipase dosage, molar ratio of PC to FA, and reaction period. Palmitic acid could be incorporated up to 58.6 and 57.1% using Lipozyme TL IM and 56 and 61% using Novozym 435 in egg and soybean PC from an initial content of 37.4 and 16.8%, respectively. Similarly, stearic acid incorporation was up to 44.7 and 46.3% using Lipozyme TL IM and 37.2 and 55.8% using Novozym 435 in egg and soybean PC from an initial content of 8.6 and 2.1%, respectively.
doi_str_mv 10.1007/s11746-005-1134-x
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R. C. ; Vijeeta, T. ; Karuna, M. S. L. ; Rao, B. V. S. K. ; Prasad, R. B. N.</creator><creatorcontrib>Reddy, J. R. C. ; Vijeeta, T. ; Karuna, M. S. L. ; Rao, B. V. S. K. ; Prasad, R. B. N.</creatorcontrib><description>Saturated FA enhance the oxidative stability of phospholipids. In the present study phosphatidylcholine (PC) rich in palmitic and stearic acids was prepared using lipase‐catalyzed transesterification from PC isolated from egg and soybean lecithins. Two different lipases, namely, Novozym 435 and Lipozyme TL IM, were used for the transesterification. The reaction conditions were optimized by varying the lipase dosage, molar ratio of PC to FA, and reaction period. Palmitic acid could be incorporated up to 58.6 and 57.1% using Lipozyme TL IM and 56 and 61% using Novozym 435 in egg and soybean PC from an initial content of 37.4 and 16.8%, respectively. 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subjects Biochemistry
Biological and medical sciences
Enzymes
Fat industries
Food industries
Fundamental and applied biological sciences. Psychology
Lipids
Lipozyme TL IM
Novozyme 435
palmitic acid
phosphatidylcholine
Soybeans
stearic acid
transesterification
Vegetable oils
title Lipase‐catalyzed preparation of palmitic and stearic acid‐rich phosphatidylcholine
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