Localization of artemisinin and artemisitene in foliar tissues of glanded and glandless biotypes of Artemisia annua L
The tissue localization of the antimalarial sesquiterpenoid compound artemisinin in annual wormwood (Artemisia annua L.) was determined by differential extraction of a glanded biotype and through the use of a glandless biotype. A 5-s dip in chloroform extracted 97% of the artemisinin from glanded A....
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| Veröffentlicht in: | International journal of plant sciences 1994-05, Vol.155 (3), p.365-372 |
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| container_title | International journal of plant sciences |
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| creator | Duke, Mary V. Paul, Rex N. Elsohly, Hala N. Sturtz, George Duke, Stephen O. |
| description | The tissue localization of the antimalarial sesquiterpenoid compound artemisinin in annual wormwood (Artemisia annua L.) was determined by differential extraction of a glanded biotype and through the use of a glandless biotype. A 5-s dip in chloroform extracted 97% of the artemisinin from glanded A. annua leaf tissue. In addition, all of the detectable artemisitene, an artemisinin analog, was extracted. This extraction method caused collapse of the subcuticular space of the capitate glands on the leaf surface, whereas no other damage to the leaf surface was observed with SEM. Light microscopy and TEM revealed that this extraction method, despite causing some organelle structural changes, did not disrupt cell membranes. An A. annua biotype without glands contained neither artemisinin nor artemisitene. These results indicate that artemisinin and artemisitene present in foliar tissue are localized entirely in the subcuticular space of capitate glands of A. annua. |
| doi_str_mv | 10.1086/297173 |
| format | Article |
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VDLUFA-Kongress. Jena (Germany). 19-24 Sep 1994 ; USDA, ARS, Southern Weed Science Laboratory, Stoneville, MS</creatorcontrib><description>The tissue localization of the antimalarial sesquiterpenoid compound artemisinin in annual wormwood (Artemisia annua L.) was determined by differential extraction of a glanded biotype and through the use of a glandless biotype. A 5-s dip in chloroform extracted 97% of the artemisinin from glanded A. annua leaf tissue. In addition, all of the detectable artemisitene, an artemisinin analog, was extracted. This extraction method caused collapse of the subcuticular space of the capitate glands on the leaf surface, whereas no other damage to the leaf surface was observed with SEM. Light microscopy and TEM revealed that this extraction method, despite causing some organelle structural changes, did not disrupt cell membranes. An A. annua biotype without glands contained neither artemisinin nor artemisitene. These results indicate that artemisinin and artemisitene present in foliar tissue are localized entirely in the subcuticular space of capitate glands of A. annua.</description><identifier>ISSN: 1058-5893</identifier><identifier>EISSN: 1537-5315</identifier><identifier>DOI: 10.1086/297173</identifier><language>eng</language><publisher>Chicago: The University of Chicago Press</publisher><subject>Antimalarials ; antiprotozoaire ; antiprotozoal agents ; artemisia ; Artemisinins ; biotipos ; biotype ; biotypes ; Botany ; chemistry ; chimie ; Chloroplasts ; Cytochemistry ; Drugs ; feuille ; Flowers & plants ; glande vegetale ; glandulas vegetales ; hojas ; immunologie ; immunology ; inmunologia ; Leaves ; Malaria ; medicamentos contra protozoarios ; Plant cells ; Plant glands ; Plants ; quimica ; Seeds ; terpenoide ; terpenoidos ; Terpenoids ; Tobacco chewing ; Trichomes</subject><ispartof>International journal of plant sciences, 1994-05, Vol.155 (3), p.365-372</ispartof><rights>Copyright 1994 The University of Chicago</rights><rights>Copyright University of Chicago, acting through its Press May 1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-a50d79a5e0c2b6aa6009482f9ac275232f85f0d1e98bbf018149f0489d0e19a63</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2475187$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2475187$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids></links><search><creatorcontrib>Duke, Mary V.</creatorcontrib><creatorcontrib>Paul, Rex N.</creatorcontrib><creatorcontrib>Elsohly, Hala N.</creatorcontrib><creatorcontrib>Sturtz, George</creatorcontrib><creatorcontrib>Duke, Stephen O.</creatorcontrib><creatorcontrib>106. VDLUFA-Kongress. Jena (Germany). 19-24 Sep 1994</creatorcontrib><creatorcontrib>USDA, ARS, Southern Weed Science Laboratory, Stoneville, MS</creatorcontrib><title>Localization of artemisinin and artemisitene in foliar tissues of glanded and glandless biotypes of Artemisia annua L</title><title>International journal of plant sciences</title><description>The tissue localization of the antimalarial sesquiterpenoid compound artemisinin in annual wormwood (Artemisia annua L.) was determined by differential extraction of a glanded biotype and through the use of a glandless biotype. A 5-s dip in chloroform extracted 97% of the artemisinin from glanded A. annua leaf tissue. In addition, all of the detectable artemisitene, an artemisinin analog, was extracted. This extraction method caused collapse of the subcuticular space of the capitate glands on the leaf surface, whereas no other damage to the leaf surface was observed with SEM. Light microscopy and TEM revealed that this extraction method, despite causing some organelle structural changes, did not disrupt cell membranes. An A. annua biotype without glands contained neither artemisinin nor artemisitene. These results indicate that artemisinin and artemisitene present in foliar tissue are localized entirely in the subcuticular space of capitate glands of A. annua.</description><subject>Antimalarials</subject><subject>antiprotozoaire</subject><subject>antiprotozoal agents</subject><subject>artemisia</subject><subject>Artemisinins</subject><subject>biotipos</subject><subject>biotype</subject><subject>biotypes</subject><subject>Botany</subject><subject>chemistry</subject><subject>chimie</subject><subject>Chloroplasts</subject><subject>Cytochemistry</subject><subject>Drugs</subject><subject>feuille</subject><subject>Flowers & plants</subject><subject>glande vegetale</subject><subject>glandulas vegetales</subject><subject>hojas</subject><subject>immunologie</subject><subject>immunology</subject><subject>inmunologia</subject><subject>Leaves</subject><subject>Malaria</subject><subject>medicamentos contra protozoarios</subject><subject>Plant cells</subject><subject>Plant glands</subject><subject>Plants</subject><subject>quimica</subject><subject>Seeds</subject><subject>terpenoide</subject><subject>terpenoidos</subject><subject>Terpenoids</subject><subject>Tobacco chewing</subject><subject>Trichomes</subject><issn>1058-5893</issn><issn>1537-5315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNo90c1PwyAUAHBiNHFO_QuMaTx4q_JRWjgui19JEw-6M3ltYWHpygR6mH-9bDU78eD9eLw8ELol-IlgUT5TWZGKnaEZ4azKOSP8PMWYi5wLyS7RVQgbjLHkVM7QWLsWevsL0bohcyYDH_XWBjvYIYOhO-2jHnSWzozrLfgs2hBGHQ431n1yujvqY9zrELLGurjfTWLxXwOSGUbI6mt0YaAP-uZ_naPV68v38j2vP98-los6bxkXMQeOu0oC17ilTQlQpq4LQY2EllacMmoEN7gjWoqmMZgIUkiDCyE7rImEks3Rw1R3591PajeqjRv9kJ5UtCoELkpJE3qcUOtdCF4btfN2C36vCFaHiappogneTXATovMnRYuKE1Gl9P2UNuAUrL0NavVFpOQ4fQQhlP0BIG17Aw</recordid><startdate>19940501</startdate><enddate>19940501</enddate><creator>Duke, Mary V.</creator><creator>Paul, Rex N.</creator><creator>Elsohly, Hala N.</creator><creator>Sturtz, George</creator><creator>Duke, Stephen O.</creator><general>The University of Chicago Press</general><general>University of Chicago, acting through its Press</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope></search><sort><creationdate>19940501</creationdate><title>Localization of artemisinin and artemisitene in foliar tissues of glanded and glandless biotypes of Artemisia annua L</title><author>Duke, Mary V. ; Paul, Rex N. ; Elsohly, Hala N. ; Sturtz, George ; Duke, Stephen O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-a50d79a5e0c2b6aa6009482f9ac275232f85f0d1e98bbf018149f0489d0e19a63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Antimalarials</topic><topic>antiprotozoaire</topic><topic>antiprotozoal agents</topic><topic>artemisia</topic><topic>Artemisinins</topic><topic>biotipos</topic><topic>biotype</topic><topic>biotypes</topic><topic>Botany</topic><topic>chemistry</topic><topic>chimie</topic><topic>Chloroplasts</topic><topic>Cytochemistry</topic><topic>Drugs</topic><topic>feuille</topic><topic>Flowers & plants</topic><topic>glande vegetale</topic><topic>glandulas vegetales</topic><topic>hojas</topic><topic>immunologie</topic><topic>immunology</topic><topic>inmunologia</topic><topic>Leaves</topic><topic>Malaria</topic><topic>medicamentos contra protozoarios</topic><topic>Plant cells</topic><topic>Plant glands</topic><topic>Plants</topic><topic>quimica</topic><topic>Seeds</topic><topic>terpenoide</topic><topic>terpenoidos</topic><topic>Terpenoids</topic><topic>Tobacco chewing</topic><topic>Trichomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duke, Mary V.</creatorcontrib><creatorcontrib>Paul, Rex N.</creatorcontrib><creatorcontrib>Elsohly, Hala N.</creatorcontrib><creatorcontrib>Sturtz, George</creatorcontrib><creatorcontrib>Duke, Stephen O.</creatorcontrib><creatorcontrib>106. VDLUFA-Kongress. Jena (Germany). 19-24 Sep 1994</creatorcontrib><creatorcontrib>USDA, ARS, Southern Weed Science Laboratory, Stoneville, MS</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>International journal of plant sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duke, Mary V.</au><au>Paul, Rex N.</au><au>Elsohly, Hala N.</au><au>Sturtz, George</au><au>Duke, Stephen O.</au><aucorp>106. VDLUFA-Kongress. Jena (Germany). 19-24 Sep 1994</aucorp><aucorp>USDA, ARS, Southern Weed Science Laboratory, Stoneville, MS</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of artemisinin and artemisitene in foliar tissues of glanded and glandless biotypes of Artemisia annua L</atitle><jtitle>International journal of plant sciences</jtitle><date>1994-05-01</date><risdate>1994</risdate><volume>155</volume><issue>3</issue><spage>365</spage><epage>372</epage><pages>365-372</pages><issn>1058-5893</issn><eissn>1537-5315</eissn><abstract>The tissue localization of the antimalarial sesquiterpenoid compound artemisinin in annual wormwood (Artemisia annua L.) was determined by differential extraction of a glanded biotype and through the use of a glandless biotype. A 5-s dip in chloroform extracted 97% of the artemisinin from glanded A. annua leaf tissue. In addition, all of the detectable artemisitene, an artemisinin analog, was extracted. This extraction method caused collapse of the subcuticular space of the capitate glands on the leaf surface, whereas no other damage to the leaf surface was observed with SEM. Light microscopy and TEM revealed that this extraction method, despite causing some organelle structural changes, did not disrupt cell membranes. An A. annua biotype without glands contained neither artemisinin nor artemisitene. These results indicate that artemisinin and artemisitene present in foliar tissue are localized entirely in the subcuticular space of capitate glands of A. annua.</abstract><cop>Chicago</cop><pub>The University of Chicago Press</pub><doi>10.1086/297173</doi><tpages>8</tpages></addata></record> |
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| subjects | Antimalarials antiprotozoaire antiprotozoal agents artemisia Artemisinins biotipos biotype biotypes Botany chemistry chimie Chloroplasts Cytochemistry Drugs feuille Flowers & plants glande vegetale glandulas vegetales hojas immunologie immunology inmunologia Leaves Malaria medicamentos contra protozoarios Plant cells Plant glands Plants quimica Seeds terpenoide terpenoidos Terpenoids Tobacco chewing Trichomes |
| title | Localization of artemisinin and artemisitene in foliar tissues of glanded and glandless biotypes of Artemisia annua L |
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