MdbHLH51 plays a positive role in anthocyanin accumulation in ‘Red Delicious’ apples
Key message MdbHLH51 played a positive regulatory role in the coloration and the accumulation of anthocyanins. Red coloring of fruit skin is an important trait of apple, mainly attributed to the accumulation of anthocyanins, which is a class of plant flavonoid metabolites widely found in plants. Our...
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| Veröffentlicht in: | Trees (Berlin, West) West), 2022-12, Vol.36 (6), p.1687-1695 |
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| creator | Ning, Gai-Xing Li, Wen-Fang Chu, Ming-Yu Ma, Zong-Huan Wang, Ping Mao, Juan Chen, Bai-Hong |
| description | Key message
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins.
Red coloring of fruit skin is an important trait of apple, mainly attributed to the accumulation of anthocyanins, which is a class of plant flavonoid metabolites widely found in plants. Our previous study carried out transcriptome sequencing in fruit skin of apple (
Malus domestica
Borkh.) cv. ‘Red Delicious’ and corroborated that bHLH members bHLH51 showed a notable positive correlation with anthocyanin content at color break stage. To our knowledge, the regulation of anthocyanin biosynthesis by
MdbHLH51
in apple fruits has not been reported so far. Therefore, it is of great significance to isolate this gene and perform further functional verification. The function of
MdbHLH51
was investigated based on the
Agrobacterium
-mediated method. In this study,
MdbHLH51
was cloned from ‘Red Delicious’ fruit skin. MdbHLH51 protein was localized to the nucleus of onion epidermal cells. Transient expression of the bHLH51-GFP in fruit skin and ‘Gala’ apple callus indicated that
MdbHLH51
promoted anthocyanin accumulation. In addition, the overexpression of
MdbHLH51
in
Arabidopsis thaliana
also could enhance the anthocyanins content and up-regulate the expression levels of the structural genes related to anthocyanins biosynthesis. Overall, these results suggested that
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins. Our results provide a useful information for understanding the function of
MdbHLH51
. |
| doi_str_mv | 10.1007/s00468-022-02342-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2742876273</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2742876273</sourcerecordid><originalsourceid>FETCH-LOGICAL-c319t-b3723975a7f3a1a9951d48b84bcae0fdc37b792c8ee6046f514123775137c8733</originalsourceid><addsrcrecordid>eNp9kM1KxDAUhYMoOI6-gKuA62j-2iRL8W-EEUEU3IU0TTVDp6lJK8xuHkNfb57EzlRw5-JyL9xzzuV-AJwSfE4wFhcJY55LhCkdinGK1B6YEM4oolRm-2CCFSOISIUPwVFKC4wxywmdgNeHspjNZxmBbW1WCRrYhuQ7_-lgDLWDvoGm6d6DXZlmO1vbL_vadD40291m_fXkSnjtam996NNm_Q1N29YuHYODytTJnfz2KXi5vXm-mqH549391eUcWUZUhwomKFMiM6JihhilMlJyWUheWONwVVomCqGolc7lw4tVRjihTIiMMGGlYGwKzsbcNoaP3qVOL0Ifm-GkpoJTKXK6U9FRZWNIKbpKt9EvTVxpgvWWoB4J6oGg3hHUajCx0ZQGcfPm4l_0P64fpGd0IA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2742876273</pqid></control><display><type>article</type><title>MdbHLH51 plays a positive role in anthocyanin accumulation in ‘Red Delicious’ apples</title><source>SpringerNature Journals</source><creator>Ning, Gai-Xing ; Li, Wen-Fang ; Chu, Ming-Yu ; Ma, Zong-Huan ; Wang, Ping ; Mao, Juan ; Chen, Bai-Hong</creator><creatorcontrib>Ning, Gai-Xing ; Li, Wen-Fang ; Chu, Ming-Yu ; Ma, Zong-Huan ; Wang, Ping ; Mao, Juan ; Chen, Bai-Hong</creatorcontrib><description>Key message
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins.
Red coloring of fruit skin is an important trait of apple, mainly attributed to the accumulation of anthocyanins, which is a class of plant flavonoid metabolites widely found in plants. Our previous study carried out transcriptome sequencing in fruit skin of apple (
Malus domestica
Borkh.) cv. ‘Red Delicious’ and corroborated that bHLH members bHLH51 showed a notable positive correlation with anthocyanin content at color break stage. To our knowledge, the regulation of anthocyanin biosynthesis by
MdbHLH51
in apple fruits has not been reported so far. Therefore, it is of great significance to isolate this gene and perform further functional verification. The function of
MdbHLH51
was investigated based on the
Agrobacterium
-mediated method. In this study,
MdbHLH51
was cloned from ‘Red Delicious’ fruit skin. MdbHLH51 protein was localized to the nucleus of onion epidermal cells. Transient expression of the bHLH51-GFP in fruit skin and ‘Gala’ apple callus indicated that
MdbHLH51
promoted anthocyanin accumulation. In addition, the overexpression of
MdbHLH51
in
Arabidopsis thaliana
also could enhance the anthocyanins content and up-regulate the expression levels of the structural genes related to anthocyanins biosynthesis. Overall, these results suggested that
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins. Our results provide a useful information for understanding the function of
MdbHLH51
.</description><identifier>ISSN: 0931-1890</identifier><identifier>EISSN: 1432-2285</identifier><identifier>DOI: 10.1007/s00468-022-02342-9</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Accumulation ; Agriculture ; Anthocyanins ; Apples ; Biomedical and Life Sciences ; Biosynthesis ; Callus ; Coloration ; Flavonoids ; Forestry ; Fruits ; Life Sciences ; Malus domestica ; Metabolites ; Plant Anatomy/Development ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Review ; Skin ; Transcriptomes</subject><ispartof>Trees (Berlin, West), 2022-12, Vol.36 (6), p.1687-1695</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-b3723975a7f3a1a9951d48b84bcae0fdc37b792c8ee6046f514123775137c8733</citedby><cites>FETCH-LOGICAL-c319t-b3723975a7f3a1a9951d48b84bcae0fdc37b792c8ee6046f514123775137c8733</cites><orcidid>0000-0002-6963-1279</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00468-022-02342-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00468-022-02342-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Ning, Gai-Xing</creatorcontrib><creatorcontrib>Li, Wen-Fang</creatorcontrib><creatorcontrib>Chu, Ming-Yu</creatorcontrib><creatorcontrib>Ma, Zong-Huan</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Mao, Juan</creatorcontrib><creatorcontrib>Chen, Bai-Hong</creatorcontrib><title>MdbHLH51 plays a positive role in anthocyanin accumulation in ‘Red Delicious’ apples</title><title>Trees (Berlin, West)</title><addtitle>Trees</addtitle><description>Key message
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins.
Red coloring of fruit skin is an important trait of apple, mainly attributed to the accumulation of anthocyanins, which is a class of plant flavonoid metabolites widely found in plants. Our previous study carried out transcriptome sequencing in fruit skin of apple (
Malus domestica
Borkh.) cv. ‘Red Delicious’ and corroborated that bHLH members bHLH51 showed a notable positive correlation with anthocyanin content at color break stage. To our knowledge, the regulation of anthocyanin biosynthesis by
MdbHLH51
in apple fruits has not been reported so far. Therefore, it is of great significance to isolate this gene and perform further functional verification. The function of
MdbHLH51
was investigated based on the
Agrobacterium
-mediated method. In this study,
MdbHLH51
was cloned from ‘Red Delicious’ fruit skin. MdbHLH51 protein was localized to the nucleus of onion epidermal cells. Transient expression of the bHLH51-GFP in fruit skin and ‘Gala’ apple callus indicated that
MdbHLH51
promoted anthocyanin accumulation. In addition, the overexpression of
MdbHLH51
in
Arabidopsis thaliana
also could enhance the anthocyanins content and up-regulate the expression levels of the structural genes related to anthocyanins biosynthesis. Overall, these results suggested that
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins. Our results provide a useful information for understanding the function of
MdbHLH51
.</description><subject>Accumulation</subject><subject>Agriculture</subject><subject>Anthocyanins</subject><subject>Apples</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Callus</subject><subject>Coloration</subject><subject>Flavonoids</subject><subject>Forestry</subject><subject>Fruits</subject><subject>Life Sciences</subject><subject>Malus domestica</subject><subject>Metabolites</subject><subject>Plant Anatomy/Development</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Review</subject><subject>Skin</subject><subject>Transcriptomes</subject><issn>0931-1890</issn><issn>1432-2285</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kM1KxDAUhYMoOI6-gKuA62j-2iRL8W-EEUEU3IU0TTVDp6lJK8xuHkNfb57EzlRw5-JyL9xzzuV-AJwSfE4wFhcJY55LhCkdinGK1B6YEM4oolRm-2CCFSOISIUPwVFKC4wxywmdgNeHspjNZxmBbW1WCRrYhuQ7_-lgDLWDvoGm6d6DXZlmO1vbL_vadD40291m_fXkSnjtam996NNm_Q1N29YuHYODytTJnfz2KXi5vXm-mqH549391eUcWUZUhwomKFMiM6JihhilMlJyWUheWONwVVomCqGolc7lw4tVRjihTIiMMGGlYGwKzsbcNoaP3qVOL0Ifm-GkpoJTKXK6U9FRZWNIKbpKt9EvTVxpgvWWoB4J6oGg3hHUajCx0ZQGcfPm4l_0P64fpGd0IA</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Ning, Gai-Xing</creator><creator>Li, Wen-Fang</creator><creator>Chu, Ming-Yu</creator><creator>Ma, Zong-Huan</creator><creator>Wang, Ping</creator><creator>Mao, Juan</creator><creator>Chen, Bai-Hong</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-6963-1279</orcidid></search><sort><creationdate>20221201</creationdate><title>MdbHLH51 plays a positive role in anthocyanin accumulation in ‘Red Delicious’ apples</title><author>Ning, Gai-Xing ; Li, Wen-Fang ; Chu, Ming-Yu ; Ma, Zong-Huan ; Wang, Ping ; Mao, Juan ; Chen, Bai-Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-b3723975a7f3a1a9951d48b84bcae0fdc37b792c8ee6046f514123775137c8733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Accumulation</topic><topic>Agriculture</topic><topic>Anthocyanins</topic><topic>Apples</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Callus</topic><topic>Coloration</topic><topic>Flavonoids</topic><topic>Forestry</topic><topic>Fruits</topic><topic>Life Sciences</topic><topic>Malus domestica</topic><topic>Metabolites</topic><topic>Plant Anatomy/Development</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Review</topic><topic>Skin</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ning, Gai-Xing</creatorcontrib><creatorcontrib>Li, Wen-Fang</creatorcontrib><creatorcontrib>Chu, Ming-Yu</creatorcontrib><creatorcontrib>Ma, Zong-Huan</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Mao, Juan</creatorcontrib><creatorcontrib>Chen, Bai-Hong</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environment Abstracts</collection><jtitle>Trees (Berlin, West)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ning, Gai-Xing</au><au>Li, Wen-Fang</au><au>Chu, Ming-Yu</au><au>Ma, Zong-Huan</au><au>Wang, Ping</au><au>Mao, Juan</au><au>Chen, Bai-Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MdbHLH51 plays a positive role in anthocyanin accumulation in ‘Red Delicious’ apples</atitle><jtitle>Trees (Berlin, West)</jtitle><stitle>Trees</stitle><date>2022-12-01</date><risdate>2022</risdate><volume>36</volume><issue>6</issue><spage>1687</spage><epage>1695</epage><pages>1687-1695</pages><issn>0931-1890</issn><eissn>1432-2285</eissn><abstract>Key message
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins.
Red coloring of fruit skin is an important trait of apple, mainly attributed to the accumulation of anthocyanins, which is a class of plant flavonoid metabolites widely found in plants. Our previous study carried out transcriptome sequencing in fruit skin of apple (
Malus domestica
Borkh.) cv. ‘Red Delicious’ and corroborated that bHLH members bHLH51 showed a notable positive correlation with anthocyanin content at color break stage. To our knowledge, the regulation of anthocyanin biosynthesis by
MdbHLH51
in apple fruits has not been reported so far. Therefore, it is of great significance to isolate this gene and perform further functional verification. The function of
MdbHLH51
was investigated based on the
Agrobacterium
-mediated method. In this study,
MdbHLH51
was cloned from ‘Red Delicious’ fruit skin. MdbHLH51 protein was localized to the nucleus of onion epidermal cells. Transient expression of the bHLH51-GFP in fruit skin and ‘Gala’ apple callus indicated that
MdbHLH51
promoted anthocyanin accumulation. In addition, the overexpression of
MdbHLH51
in
Arabidopsis thaliana
also could enhance the anthocyanins content and up-regulate the expression levels of the structural genes related to anthocyanins biosynthesis. Overall, these results suggested that
MdbHLH51
played a positive regulatory role in the coloration and the accumulation of anthocyanins. Our results provide a useful information for understanding the function of
MdbHLH51
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00468-022-02342-9</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-6963-1279</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0931-1890 |
| ispartof | Trees (Berlin, West), 2022-12, Vol.36 (6), p.1687-1695 |
| issn | 0931-1890 1432-2285 |
| language | eng |
| recordid | cdi_proquest_journals_2742876273 |
| source | SpringerNature Journals |
| subjects | Accumulation Agriculture Anthocyanins Apples Biomedical and Life Sciences Biosynthesis Callus Coloration Flavonoids Forestry Fruits Life Sciences Malus domestica Metabolites Plant Anatomy/Development Plant Pathology Plant Physiology Plant Sciences Review Skin Transcriptomes |
| title | MdbHLH51 plays a positive role in anthocyanin accumulation in ‘Red Delicious’ apples |
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