THE EFFECT OF A SINGLE APPLICATION OF DIFFERENT FLUORIDE VARNISHES ON ENAMEL SUBSURFACE LESIONS IN VITRO
This in vitro study aimed to evaluate the therapeutic effect of different fluoride ion (F) varnish formulations for controlling the carious development of enamel subsurface lesions and the F release into artificial saliva for 2 hr, 24 hr, 48 hr, and 7 days. Artificial enamel carious lesions were cre...
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| Veröffentlicht in: | Fluoride 2022-04, Vol.55 (2), p.121-130 |
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| creator | Yildiz, Pinar Kulan Yavuz, Betul Sen Yilmaz, Muesser Ahu Oktay, Nihal Sehkar Kargul, Betul |
| description | This in vitro study aimed to evaluate the therapeutic effect of different fluoride ion (F) varnish formulations for controlling the carious development of enamel subsurface lesions and the F release into artificial saliva for 2 hr, 24 hr, 48 hr, and 7 days. Artificial enamel carious lesions were created and divided into 6 groups (5 varnish groups and a control group). Varnishes were applied to enamel specimens and then the specimens were incubated in artificial saliva, with the artificial saliva replenished daily. Varnish was removed and lesions were remineralized in artificial saliva for 24 hr. Surface microhardness was measured three times: (i) initially, (ii) after creating the artificial enamel lesions, and (iii) after applying the varnishes. The F release was analyzed after 2 hr, 24 hr, 48 hr, and 7 days of exposure using an ion-selective electrode. Data were analyzed using a One-way Analysis of Variance with the Tukey-Kramer Multiple Comparisons test and the Kruskal-Wallis test with the Dunns Multiple Comparisons test. The highest percentage surface microhardness recovery was found for the treatment with the MI Varnish. According to the percentage surface microhardness recovery results, a statistically significant difference was found between the varnishes and the control group (p |
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Artificial enamel carious lesions were created and divided into 6 groups (5 varnish groups and a control group). Varnishes were applied to enamel specimens and then the specimens were incubated in artificial saliva, with the artificial saliva replenished daily. Varnish was removed and lesions were remineralized in artificial saliva for 24 hr. Surface microhardness was measured three times: (i) initially, (ii) after creating the artificial enamel lesions, and (iii) after applying the varnishes. The F release was analyzed after 2 hr, 24 hr, 48 hr, and 7 days of exposure using an ion-selective electrode. Data were analyzed using a One-way Analysis of Variance with the Tukey-Kramer Multiple Comparisons test and the Kruskal-Wallis test with the Dunns Multiple Comparisons test. The highest percentage surface microhardness recovery was found for the treatment with the MI Varnish. According to the percentage surface microhardness recovery results, a statistically significant difference was found between the varnishes and the control group (p<0.05 and <0.001). All varnishes released measurable levels of fluoride ions. However, the release of F was the highest in the MI Varnish group (p<0.01). Duraphat, Enamel Pro Varnish, and MI Varnish released the most F into artificial saliva. Calcium phosphate-based F varnishes improve the capacity of the enamel surface rehardening. CPP containing F varnish had the highest release of F as compared to the other F releasing varnishes. Further in vivo investigations are also required to prove the clinical applications of the different ingredients containing varnishes.</description><identifier>ISSN: 0015-4725</identifier><identifier>EISSN: 2253-4083</identifier><language>eng</language><publisher>Dunedin: International Society for Fluoride Research, Inc</publisher><subject>Calcium phosphates ; Dental caries ; Enamel ; Fluorides ; Kruskal-Wallis test ; Variance analysis</subject><ispartof>Fluoride, 2022-04, Vol.55 (2), p.121-130</ispartof><rights>2022. This work is published under http://www.fluorideresearch.org/ (the “License”). 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Artificial enamel carious lesions were created and divided into 6 groups (5 varnish groups and a control group). Varnishes were applied to enamel specimens and then the specimens were incubated in artificial saliva, with the artificial saliva replenished daily. Varnish was removed and lesions were remineralized in artificial saliva for 24 hr. Surface microhardness was measured three times: (i) initially, (ii) after creating the artificial enamel lesions, and (iii) after applying the varnishes. The F release was analyzed after 2 hr, 24 hr, 48 hr, and 7 days of exposure using an ion-selective electrode. Data were analyzed using a One-way Analysis of Variance with the Tukey-Kramer Multiple Comparisons test and the Kruskal-Wallis test with the Dunns Multiple Comparisons test. The highest percentage surface microhardness recovery was found for the treatment with the MI Varnish. According to the percentage surface microhardness recovery results, a statistically significant difference was found between the varnishes and the control group (p<0.05 and <0.001). All varnishes released measurable levels of fluoride ions. However, the release of F was the highest in the MI Varnish group (p<0.01). Duraphat, Enamel Pro Varnish, and MI Varnish released the most F into artificial saliva. Calcium phosphate-based F varnishes improve the capacity of the enamel surface rehardening. CPP containing F varnish had the highest release of F as compared to the other F releasing varnishes. Further in vivo investigations are also required to prove the clinical applications of the different ingredients containing varnishes.</description><subject>Calcium phosphates</subject><subject>Dental caries</subject><subject>Enamel</subject><subject>Fluorides</subject><subject>Kruskal-Wallis test</subject><subject>Variance analysis</subject><issn>0015-4725</issn><issn>2253-4083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNotj99LwzAcxIMoWKf_Q8DnQn702zaPtUu2QExHk-51pE2LDHFz3f5_K_p0cNzdh7tDCWPA04yU_B4lhFBIs4LBI3qa5yMhXFAuEvThtxJLpWTtcaNwhZ22GyNxtdsZXVdeN_bXX-sl0krrsTJd0-q1xPuqtdptpcNLRNrqXRrsujfXtaqqJTbSLV2HtcV77dvmGT1M4XMeX_51hTolfb1NTbNZQCY9U8qvKROsoGXJph4CxDEbQcS-JzTmNA8DCUWMJQQiQMBYDCQnPWRhgjLkIcZxGPgKvf7tni-n79s4Xw_H0-3ytSAPrMiW2zzLgf8AAPlLAw</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Yildiz, Pinar Kulan</creator><creator>Yavuz, Betul Sen</creator><creator>Yilmaz, Muesser Ahu</creator><creator>Oktay, Nihal Sehkar</creator><creator>Kargul, Betul</creator><general>International Society for Fluoride Research, Inc</general><scope>3V.</scope><scope>7XB</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AYAGU</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PADUT</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20220401</creationdate><title>THE EFFECT OF A SINGLE APPLICATION OF DIFFERENT FLUORIDE VARNISHES ON ENAMEL SUBSURFACE LESIONS IN VITRO</title><author>Yildiz, Pinar Kulan ; 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Artificial enamel carious lesions were created and divided into 6 groups (5 varnish groups and a control group). Varnishes were applied to enamel specimens and then the specimens were incubated in artificial saliva, with the artificial saliva replenished daily. Varnish was removed and lesions were remineralized in artificial saliva for 24 hr. Surface microhardness was measured three times: (i) initially, (ii) after creating the artificial enamel lesions, and (iii) after applying the varnishes. The F release was analyzed after 2 hr, 24 hr, 48 hr, and 7 days of exposure using an ion-selective electrode. Data were analyzed using a One-way Analysis of Variance with the Tukey-Kramer Multiple Comparisons test and the Kruskal-Wallis test with the Dunns Multiple Comparisons test. The highest percentage surface microhardness recovery was found for the treatment with the MI Varnish. According to the percentage surface microhardness recovery results, a statistically significant difference was found between the varnishes and the control group (p<0.05 and <0.001). All varnishes released measurable levels of fluoride ions. However, the release of F was the highest in the MI Varnish group (p<0.01). Duraphat, Enamel Pro Varnish, and MI Varnish released the most F into artificial saliva. Calcium phosphate-based F varnishes improve the capacity of the enamel surface rehardening. CPP containing F varnish had the highest release of F as compared to the other F releasing varnishes. Further in vivo investigations are also required to prove the clinical applications of the different ingredients containing varnishes.</abstract><cop>Dunedin</cop><pub>International Society for Fluoride Research, Inc</pub><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Calcium phosphates Dental caries Enamel Fluorides Kruskal-Wallis test Variance analysis |
| title | THE EFFECT OF A SINGLE APPLICATION OF DIFFERENT FLUORIDE VARNISHES ON ENAMEL SUBSURFACE LESIONS IN VITRO |
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