Self-propelled Janus nanomotor as active probe for detection of pepsinogen by lateral flow immunoassay
The feasibility of using nanomotors as active probes for lateral flow immunoassay (LFIA) is demonstrated. We synthesized Au@mSiO 2 @Pt Janus nanomotor, where nanolayer of Pt was deposited on the half side surface of the Au@mSiO 2 nanoparticles, which can catalyze the decomposition of H 2 O 2 to prod...
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| Veröffentlicht in: | Mikrochimica acta (1966) 2022-12, Vol.189 (12), p.468, Article 468 |
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| creator | Guo, Jiuchuan Li, Yang Wang, Bo Chen, Wenjun Chen, Shuqin Liu, Shan Ma, Xing Guo, Jinhong |
| description | The feasibility of using nanomotors as active probes for lateral flow immunoassay (LFIA) is demonstrated. We synthesized Au@mSiO
2
@Pt Janus nanomotor, where nanolayer of Pt was deposited on the half side surface of the Au@mSiO
2
nanoparticles, which can catalyze the decomposition of H
2
O
2
to produce driving force for the nanomotor. Subsequently, the motion characteristics of the Au@mSiO
2
@Pt nanomotor in static fluidic environment and dynamic flow field was studied to pave the way for its practical application in lateral flow immunoassay (LFIA). At last, the Au@mSiO
2
@Pt nanomotor was modified with antibody and then used as active immunoassay probe in LFIA. We chose gastric function index, pepsinogen II (PG II) and pepsinogen II (PG II), as the target analytes. The results indicated that, compared with traditional Au nanoprobe, the nanomotor-based probe can significantly improve the sensitivity by increasing the probability and efficiency of antigen and antibody binding. A limit of detection (LOD) of 2.2 ng/mL for PGI, and 2.1 ng/mL for PG II was achieved. This work provides a new solution for enhancing the capability of immune detection, and we believe the nanomotor-based LFIA will have great potential in high-sensitivity point-of-care-testing in the future.
Graphical Abstract |
| doi_str_mv | 10.1007/s00604-022-05538-5 |
| format | Article |
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2
@Pt Janus nanomotor, where nanolayer of Pt was deposited on the half side surface of the Au@mSiO
2
nanoparticles, which can catalyze the decomposition of H
2
O
2
to produce driving force for the nanomotor. Subsequently, the motion characteristics of the Au@mSiO
2
@Pt nanomotor in static fluidic environment and dynamic flow field was studied to pave the way for its practical application in lateral flow immunoassay (LFIA). At last, the Au@mSiO
2
@Pt nanomotor was modified with antibody and then used as active immunoassay probe in LFIA. We chose gastric function index, pepsinogen II (PG II) and pepsinogen II (PG II), as the target analytes. The results indicated that, compared with traditional Au nanoprobe, the nanomotor-based probe can significantly improve the sensitivity by increasing the probability and efficiency of antigen and antibody binding. A limit of detection (LOD) of 2.2 ng/mL for PGI, and 2.1 ng/mL for PG II was achieved. This work provides a new solution for enhancing the capability of immune detection, and we believe the nanomotor-based LFIA will have great potential in high-sensitivity point-of-care-testing in the future.
Graphical Abstract</description><identifier>ISSN: 0026-3672</identifier><identifier>EISSN: 1436-5073</identifier><identifier>DOI: 10.1007/s00604-022-05538-5</identifier><identifier>PMID: 36422710</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Analytical Chemistry ; Antibodies ; Antigens ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Gold ; Hydrogen Peroxide ; Immunoassay ; Immunoassay - methods ; Investigations ; Limit of Detection ; Microengineering ; Mutual fund industry ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Nanotechnology devices ; Original Paper ; Pepsinogen A ; Pepsinogen C ; Platinum ; Sensitivity ; Viral antibodies</subject><ispartof>Mikrochimica acta (1966), 2022-12, Vol.189 (12), p.468, Article 468</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.</rights><rights>COPYRIGHT 2022 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-c86ec606ee2f4c10e66e60bfa6b978b6f34493757c9e330c1e2ae7464cf6d19a3</citedby><cites>FETCH-LOGICAL-c442t-c86ec606ee2f4c10e66e60bfa6b978b6f34493757c9e330c1e2ae7464cf6d19a3</cites><orcidid>0000-0002-2248-4806</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00604-022-05538-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00604-022-05538-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36422710$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Jiuchuan</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Chen, Wenjun</creatorcontrib><creatorcontrib>Chen, Shuqin</creatorcontrib><creatorcontrib>Liu, Shan</creatorcontrib><creatorcontrib>Ma, Xing</creatorcontrib><creatorcontrib>Guo, Jinhong</creatorcontrib><title>Self-propelled Janus nanomotor as active probe for detection of pepsinogen by lateral flow immunoassay</title><title>Mikrochimica acta (1966)</title><addtitle>Microchim Acta</addtitle><addtitle>Mikrochim Acta</addtitle><description>The feasibility of using nanomotors as active probes for lateral flow immunoassay (LFIA) is demonstrated. We synthesized Au@mSiO
2
@Pt Janus nanomotor, where nanolayer of Pt was deposited on the half side surface of the Au@mSiO
2
nanoparticles, which can catalyze the decomposition of H
2
O
2
to produce driving force for the nanomotor. Subsequently, the motion characteristics of the Au@mSiO
2
@Pt nanomotor in static fluidic environment and dynamic flow field was studied to pave the way for its practical application in lateral flow immunoassay (LFIA). At last, the Au@mSiO
2
@Pt nanomotor was modified with antibody and then used as active immunoassay probe in LFIA. We chose gastric function index, pepsinogen II (PG II) and pepsinogen II (PG II), as the target analytes. The results indicated that, compared with traditional Au nanoprobe, the nanomotor-based probe can significantly improve the sensitivity by increasing the probability and efficiency of antigen and antibody binding. A limit of detection (LOD) of 2.2 ng/mL for PGI, and 2.1 ng/mL for PG II was achieved. This work provides a new solution for enhancing the capability of immune detection, and we believe the nanomotor-based LFIA will have great potential in high-sensitivity point-of-care-testing in the future.
Graphical Abstract</description><subject>Analytical Chemistry</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Gold</subject><subject>Hydrogen Peroxide</subject><subject>Immunoassay</subject><subject>Immunoassay - methods</subject><subject>Investigations</subject><subject>Limit of Detection</subject><subject>Microengineering</subject><subject>Mutual fund industry</subject><subject>Nanochemistry</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Nanotechnology devices</subject><subject>Original Paper</subject><subject>Pepsinogen A</subject><subject>Pepsinogen C</subject><subject>Platinum</subject><subject>Sensitivity</subject><subject>Viral antibodies</subject><issn>0026-3672</issn><issn>1436-5073</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtr3TAQhUVpaW7S_oEuiqBrpaOHJXsZQl8hkEWStZDl0cXBllzJbrn_vmpv2lAoZRYDR98ZzXAIecPhnAOY9wVAg2IgBIOmkS1rnpEdV1KzBox8TnYAQjOpjTghp6U8AHCjhXpJTqRWQhgOOxJucQpsyWnBacKBXrm4FRpdTHNaU6auUOfX8RvSyvRIQ9UGXLFqKdIU6IJLGWPaY6T9gU5uxewmGqb0nY7zvMXkSnGHV-RFcFPB14_9jNx__HB3-Zld33z6cnlxzbxSYmW-1eg1aEQRlOeAWqOGPjjdd6btdZBKddI0xncoJXiOwqFRWvmgB945eUbeHefWbb9uWFb7kLYc65dWGNnV83Wrn6i9m9COMaQ1Oz-PxdsLI0zLG8Flpc7_QdUacB59ihjGqv9lEEeDz6mUjMEueZxdPlgO9mdi9piYrYnZX4nZpprePm689TMOfyy_I6qAPAKlPsU95qeT_jP2BwUaoIg</recordid><startdate>20221201</startdate><enddate>20221201</enddate><creator>Guo, Jiuchuan</creator><creator>Li, Yang</creator><creator>Wang, Bo</creator><creator>Chen, Wenjun</creator><creator>Chen, Shuqin</creator><creator>Liu, Shan</creator><creator>Ma, Xing</creator><creator>Guo, Jinhong</creator><general>Springer Vienna</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-2248-4806</orcidid></search><sort><creationdate>20221201</creationdate><title>Self-propelled Janus nanomotor as active probe for detection of pepsinogen by lateral flow immunoassay</title><author>Guo, Jiuchuan ; Li, Yang ; Wang, Bo ; Chen, Wenjun ; Chen, Shuqin ; Liu, Shan ; Ma, Xing ; Guo, Jinhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-c86ec606ee2f4c10e66e60bfa6b978b6f34493757c9e330c1e2ae7464cf6d19a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analytical Chemistry</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Characterization and Evaluation of Materials</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Gold</topic><topic>Hydrogen Peroxide</topic><topic>Immunoassay</topic><topic>Immunoassay - methods</topic><topic>Investigations</topic><topic>Limit of Detection</topic><topic>Microengineering</topic><topic>Mutual fund industry</topic><topic>Nanochemistry</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Nanotechnology devices</topic><topic>Original Paper</topic><topic>Pepsinogen A</topic><topic>Pepsinogen C</topic><topic>Platinum</topic><topic>Sensitivity</topic><topic>Viral antibodies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Jiuchuan</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Chen, Wenjun</creatorcontrib><creatorcontrib>Chen, Shuqin</creatorcontrib><creatorcontrib>Liu, Shan</creatorcontrib><creatorcontrib>Ma, Xing</creatorcontrib><creatorcontrib>Guo, Jinhong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Mikrochimica acta (1966)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Jiuchuan</au><au>Li, Yang</au><au>Wang, Bo</au><au>Chen, Wenjun</au><au>Chen, Shuqin</au><au>Liu, Shan</au><au>Ma, Xing</au><au>Guo, Jinhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Self-propelled Janus nanomotor as active probe for detection of pepsinogen by lateral flow immunoassay</atitle><jtitle>Mikrochimica acta (1966)</jtitle><stitle>Microchim Acta</stitle><addtitle>Mikrochim Acta</addtitle><date>2022-12-01</date><risdate>2022</risdate><volume>189</volume><issue>12</issue><spage>468</spage><pages>468-</pages><artnum>468</artnum><issn>0026-3672</issn><eissn>1436-5073</eissn><abstract>The feasibility of using nanomotors as active probes for lateral flow immunoassay (LFIA) is demonstrated. We synthesized Au@mSiO
2
@Pt Janus nanomotor, where nanolayer of Pt was deposited on the half side surface of the Au@mSiO
2
nanoparticles, which can catalyze the decomposition of H
2
O
2
to produce driving force for the nanomotor. Subsequently, the motion characteristics of the Au@mSiO
2
@Pt nanomotor in static fluidic environment and dynamic flow field was studied to pave the way for its practical application in lateral flow immunoassay (LFIA). At last, the Au@mSiO
2
@Pt nanomotor was modified with antibody and then used as active immunoassay probe in LFIA. We chose gastric function index, pepsinogen II (PG II) and pepsinogen II (PG II), as the target analytes. The results indicated that, compared with traditional Au nanoprobe, the nanomotor-based probe can significantly improve the sensitivity by increasing the probability and efficiency of antigen and antibody binding. A limit of detection (LOD) of 2.2 ng/mL for PGI, and 2.1 ng/mL for PG II was achieved. This work provides a new solution for enhancing the capability of immune detection, and we believe the nanomotor-based LFIA will have great potential in high-sensitivity point-of-care-testing in the future.
Graphical Abstract</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>36422710</pmid><doi>10.1007/s00604-022-05538-5</doi><orcidid>https://orcid.org/0000-0002-2248-4806</orcidid></addata></record> |
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| subjects | Analytical Chemistry Antibodies Antigens Characterization and Evaluation of Materials Chemistry Chemistry and Materials Science Gold Hydrogen Peroxide Immunoassay Immunoassay - methods Investigations Limit of Detection Microengineering Mutual fund industry Nanochemistry Nanoparticles Nanotechnology Nanotechnology devices Original Paper Pepsinogen A Pepsinogen C Platinum Sensitivity Viral antibodies |
| title | Self-propelled Janus nanomotor as active probe for detection of pepsinogen by lateral flow immunoassay |
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