Simultaneous electroanalysis of adenine and adenosine on a poly-nicotinic acid modified pencil graphite electrode

This work portrays the simultaneous electrochemical analysis of adenine and adenosine on a poly-nicotinic acid modified pencil graphite electrode (poly-NA/PGE). The electrochemical behavior of the PGE and poly-NA/PGE were analyzed using cyclic voltammetry and electrochemical impedance spectroscopy. Morphological comparison of the PGE and modified PGE were done with field emission scanning electron microscopy and atomic force microscopy. Faradaic response of adenine and adenosine were observed on poly-NA/PGE at 0.95 V and 1.23 V, respectively, at neutral pH. Using differential pulse voltammetric technique, the sensor demonstrated a linear response to both adenine and adenosine from 2.0—70 µM concentration range with lower detection limits of 0.9276 μM and 0.8794 μM, respectively. The feasibility of the developed electrochemical device was also studied in blood serum and urine samples and was found to be a remarkable tool for the simultaneous electroanalysis of adenine and adenosine. Graphical abstract