Rutin Ameliorates H2O2-Induced Oxidative Stress Injury in HaCaT Cells via the Nrf2-Regulated Pathway
Oxidative stress induced by harmful substances activates inflammatory signaling pathways and causes excessive proliferation of keratinocytes, which is related to the occurrence of psoriasis. Rutin, a natural citrus flavonoid glycoside, exhibits protective effects against oxidative stress. However, w...
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| Veröffentlicht in: | Journal of evolutionary biochemistry and physiology 2022-09, Vol.58 (5), p.1389-1400 |
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| container_title | Journal of evolutionary biochemistry and physiology |
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| creator | Lang, G.-P. Han, Y.-Y. |
| description | Oxidative stress induced by harmful substances activates inflammatory signaling pathways and causes excessive proliferation of keratinocytes, which is related to the occurrence of psoriasis. Rutin, a natural citrus flavonoid glycoside, exhibits protective effects against oxidative stress. However, whether rutin is able to influence oxidative stress in keratinocytes remains unclear. In the present study, an in vitro cell model of human keratinocytes (HaCaT) treated with H
2
O
2
was used to explore whether rutin can prevent oxidative stress. The present findings suggest that rutin protected HaCaT cells against oxidative damage by inhibiting ROS, NO and MDA secretion, increasing SOD activity and restoring GSH-Px activity. In addition, rutin supplement limited IL-6, IL-1β and IL-23A production in HaCaT cells. Moreover, rutin upregulated Nrf2 expression, and promoted the downstream NQO1 and HO-1 expression. These results suggest that rutin may inhibit HaCaT cell oxidative stress by modulating the Nrf2-regulated pathway. |
| doi_str_mv | 10.1134/S0022093022050106 |
| format | Article |
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2
O
2
was used to explore whether rutin can prevent oxidative stress. The present findings suggest that rutin protected HaCaT cells against oxidative damage by inhibiting ROS, NO and MDA secretion, increasing SOD activity and restoring GSH-Px activity. In addition, rutin supplement limited IL-6, IL-1β and IL-23A production in HaCaT cells. Moreover, rutin upregulated Nrf2 expression, and promoted the downstream NQO1 and HO-1 expression. These results suggest that rutin may inhibit HaCaT cell oxidative stress by modulating the Nrf2-regulated pathway.</description><identifier>ISSN: 0022-0930</identifier><identifier>EISSN: 1608-3202</identifier><identifier>DOI: 10.1134/S0022093022050106</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Animal Physiology ; Biochemistry ; Biomedical and Life Sciences ; Evolutionary Biology ; Experimental Papers ; Flavonoids ; Hydrogen peroxide ; IL-1β ; Inflammation ; Interleukin 6 ; Keratinocytes ; Life Sciences ; Oxidative stress ; Psoriasis ; Rutin ; Superoxide dismutase</subject><ispartof>Journal of evolutionary biochemistry and physiology, 2022-09, Vol.58 (5), p.1389-1400</ispartof><rights>Pleiades Publishing, Ltd. 2022</rights><rights>Pleiades Publishing, Ltd. 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c294t-df562620e0fe1902edc0e49775ed361db9c68e8d976d0132a2a0ca57d56c4ddb3</citedby><cites>FETCH-LOGICAL-c294t-df562620e0fe1902edc0e49775ed361db9c68e8d976d0132a2a0ca57d56c4ddb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0022093022050106$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0022093022050106$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Lang, G.-P.</creatorcontrib><creatorcontrib>Han, Y.-Y.</creatorcontrib><title>Rutin Ameliorates H2O2-Induced Oxidative Stress Injury in HaCaT Cells via the Nrf2-Regulated Pathway</title><title>Journal of evolutionary biochemistry and physiology</title><addtitle>J Evol Biochem Phys</addtitle><description>Oxidative stress induced by harmful substances activates inflammatory signaling pathways and causes excessive proliferation of keratinocytes, which is related to the occurrence of psoriasis. Rutin, a natural citrus flavonoid glycoside, exhibits protective effects against oxidative stress. However, whether rutin is able to influence oxidative stress in keratinocytes remains unclear. In the present study, an in vitro cell model of human keratinocytes (HaCaT) treated with H
2
O
2
was used to explore whether rutin can prevent oxidative stress. The present findings suggest that rutin protected HaCaT cells against oxidative damage by inhibiting ROS, NO and MDA secretion, increasing SOD activity and restoring GSH-Px activity. In addition, rutin supplement limited IL-6, IL-1β and IL-23A production in HaCaT cells. Moreover, rutin upregulated Nrf2 expression, and promoted the downstream NQO1 and HO-1 expression. These results suggest that rutin may inhibit HaCaT cell oxidative stress by modulating the Nrf2-regulated pathway.</description><subject>Animal Physiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Evolutionary Biology</subject><subject>Experimental Papers</subject><subject>Flavonoids</subject><subject>Hydrogen peroxide</subject><subject>IL-1β</subject><subject>Inflammation</subject><subject>Interleukin 6</subject><subject>Keratinocytes</subject><subject>Life Sciences</subject><subject>Oxidative stress</subject><subject>Psoriasis</subject><subject>Rutin</subject><subject>Superoxide dismutase</subject><issn>0022-0930</issn><issn>1608-3202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kEFLw0AUhBdRsFZ_gLcFz9G3u8kmeyxBbaFYaes5bLMvbUqa1N1Na_-9CRU8iJc3h5lvHgwh9wweGRPh0wKAc1CivxEwkBdkwCQkgeDAL8mgt4PevyY3zm0BQCVhOCBm3vqypqMdVmVjtUdHx3zGg0lt2hwNnX2VRvvygHThLTpHJ_W2tSfaMWOd6iVNsaocPZSa-g3SN1vwYI7rtuqqDH3XfnPUp1tyVejK4d2PDsnHy_MyHQfT2eskHU2DnKvQB6aIJJccEApkCjiaHDBUcRyhEZKZlcplgolRsTTABNdcQ66j2EQyD41ZiSF5OPfubfPZovPZtmlt3b3MeCwgBhEz1aXYOZXbxjmLRba35U7bU8Yg68fM_ozZMfzMuC5br9H-Nv8PfQNF6nSw</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Lang, G.-P.</creator><creator>Han, Y.-Y.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>20220901</creationdate><title>Rutin Ameliorates H2O2-Induced Oxidative Stress Injury in HaCaT Cells via the Nrf2-Regulated Pathway</title><author>Lang, G.-P. ; Han, Y.-Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c294t-df562620e0fe1902edc0e49775ed361db9c68e8d976d0132a2a0ca57d56c4ddb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Animal Physiology</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Evolutionary Biology</topic><topic>Experimental Papers</topic><topic>Flavonoids</topic><topic>Hydrogen peroxide</topic><topic>IL-1β</topic><topic>Inflammation</topic><topic>Interleukin 6</topic><topic>Keratinocytes</topic><topic>Life Sciences</topic><topic>Oxidative stress</topic><topic>Psoriasis</topic><topic>Rutin</topic><topic>Superoxide dismutase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lang, G.-P.</creatorcontrib><creatorcontrib>Han, Y.-Y.</creatorcontrib><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of evolutionary biochemistry and physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lang, G.-P.</au><au>Han, Y.-Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rutin Ameliorates H2O2-Induced Oxidative Stress Injury in HaCaT Cells via the Nrf2-Regulated Pathway</atitle><jtitle>Journal of evolutionary biochemistry and physiology</jtitle><stitle>J Evol Biochem Phys</stitle><date>2022-09-01</date><risdate>2022</risdate><volume>58</volume><issue>5</issue><spage>1389</spage><epage>1400</epage><pages>1389-1400</pages><issn>0022-0930</issn><eissn>1608-3202</eissn><abstract>Oxidative stress induced by harmful substances activates inflammatory signaling pathways and causes excessive proliferation of keratinocytes, which is related to the occurrence of psoriasis. Rutin, a natural citrus flavonoid glycoside, exhibits protective effects against oxidative stress. However, whether rutin is able to influence oxidative stress in keratinocytes remains unclear. In the present study, an in vitro cell model of human keratinocytes (HaCaT) treated with H
2
O
2
was used to explore whether rutin can prevent oxidative stress. The present findings suggest that rutin protected HaCaT cells against oxidative damage by inhibiting ROS, NO and MDA secretion, increasing SOD activity and restoring GSH-Px activity. In addition, rutin supplement limited IL-6, IL-1β and IL-23A production in HaCaT cells. Moreover, rutin upregulated Nrf2 expression, and promoted the downstream NQO1 and HO-1 expression. These results suggest that rutin may inhibit HaCaT cell oxidative stress by modulating the Nrf2-regulated pathway.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0022093022050106</doi><tpages>12</tpages></addata></record> |
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| subjects | Animal Physiology Biochemistry Biomedical and Life Sciences Evolutionary Biology Experimental Papers Flavonoids Hydrogen peroxide IL-1β Inflammation Interleukin 6 Keratinocytes Life Sciences Oxidative stress Psoriasis Rutin Superoxide dismutase |
| title | Rutin Ameliorates H2O2-Induced Oxidative Stress Injury in HaCaT Cells via the Nrf2-Regulated Pathway |
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