Designing new nanoliposomal formulations and evaluating their effects on myeloid‐derived suppressor cells and regulatory T cells in a colon cancer model aiming to develop an efficient delivery system for cancer treatment; an in vitro and in vivo study
Regulfatory T cells (Tregs) and myeloid‐derived suppressor cells (MDSCs) are common immunosuppressive cells in the tumor microenvironment. These cells, through various mechanisms, inhibit antitumor immune responses and impede effective therapies. Therefore, designing an efficient protocol for induci...
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| Veröffentlicht in: | Biotechnology and applied biochemistry 2022-10, Vol.69 (5), p.2151-2160 |
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| creator | Taheri, Ramezan Ali Bahramifar, Ali Jaafari, Mahmoud Reza Fasihi‐Ramandi, Mahdi Emameh, Reza Zolfaghari Mohammadian Haftcheshmeh, Saeed Ebrahimi Nik, Maryam |
| description | Regulfatory T cells (Tregs) and myeloid‐derived suppressor cells (MDSCs) are common immunosuppressive cells in the tumor microenvironment. These cells, through various mechanisms, inhibit antitumor immune responses and impede effective therapies. Therefore, designing an efficient protocol for inducing immune surveillance in tumors is highly recommended. Recently, nanoliposomes have provided broad‐spectrum and state‐of‐the‐art vehicles to deliver antigens or immune system compartments in immunotherapies. It has been shown that different lipids in the structure of liposomes and various liposomal formulations can affect immune responses in the tumor microenvironment. This study was aimed to evaluate the effects of four different liposomal formulations on MDSCs and Tregs in C26 tumor‐bearing mice. To this end, after preparing liposomes, they were injected into tumor‐inoculated mice and analyzed MDSC and Treg population and functions in spleen and tumor tissues. Results showed that 1,2‐dioleoyl‐3‐trimethylammonium propane (DOTAP)‐containing liposomes reduced MDSC population and activity in the spleen, but not tumor, compared with other groups significantly (p |
| doi_str_mv | 10.1002/bab.2275 |
| format | Article |
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These cells, through various mechanisms, inhibit antitumor immune responses and impede effective therapies. Therefore, designing an efficient protocol for inducing immune surveillance in tumors is highly recommended. Recently, nanoliposomes have provided broad‐spectrum and state‐of‐the‐art vehicles to deliver antigens or immune system compartments in immunotherapies. It has been shown that different lipids in the structure of liposomes and various liposomal formulations can affect immune responses in the tumor microenvironment. This study was aimed to evaluate the effects of four different liposomal formulations on MDSCs and Tregs in C26 tumor‐bearing mice. To this end, after preparing liposomes, they were injected into tumor‐inoculated mice and analyzed MDSC and Treg population and functions in spleen and tumor tissues. Results showed that 1,2‐dioleoyl‐3‐trimethylammonium propane (DOTAP)‐containing liposomes reduced MDSC population and activity in the spleen, but not tumor, compared with other groups significantly (p < 0.05 and p < 0.01, respectively). Moreover, DOTAP‐containing liposomes reduced the expression of S100A8 and arginase‐1 genes in splenic MDSCs (p < 0.05). In conclusion, we provided evidence that DOTAP‐containing liposomes contributed to stimulating immune responses and provided a situation to inhibit immunosuppression in the tumor microenvironment.</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1002/bab.2275</identifier><identifier>PMID: 34698408</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Antigens ; Antitumor activity ; Arginase ; cancer ; Cancer therapies ; Colon ; Colon cancer ; Colorectal cancer ; Immune system ; Immunoregulation ; Immunosuppression ; Immunosurveillance ; Immunotherapy ; In vivo methods and tests ; Lipids ; liposome ; Liposomes ; Lymphocytes ; Lymphocytes T ; MDSC ; regulatory T cell ; Spleen ; Suppressor cells ; Tumor microenvironment ; Tumors</subject><ispartof>Biotechnology and applied biochemistry, 2022-10, Vol.69 (5), p.2151-2160</ispartof><rights>2021 International Union of Biochemistry and Molecular Biology, Inc.</rights><rights>2022 International Union of Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3495-74da15106bf42fbaba05a99cfcc021d28f0b3e93cac5c341ed9001124c8c64603</citedby><cites>FETCH-LOGICAL-c3495-74da15106bf42fbaba05a99cfcc021d28f0b3e93cac5c341ed9001124c8c64603</cites><orcidid>0000-0003-1394-6412 ; 0000-0003-3908-6828 ; 0000-0002-7647-1904</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbab.2275$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbab.2275$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34698408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Taheri, Ramezan Ali</creatorcontrib><creatorcontrib>Bahramifar, Ali</creatorcontrib><creatorcontrib>Jaafari, Mahmoud Reza</creatorcontrib><creatorcontrib>Fasihi‐Ramandi, Mahdi</creatorcontrib><creatorcontrib>Emameh, Reza Zolfaghari</creatorcontrib><creatorcontrib>Mohammadian Haftcheshmeh, Saeed</creatorcontrib><creatorcontrib>Ebrahimi Nik, Maryam</creatorcontrib><title>Designing new nanoliposomal formulations and evaluating their effects on myeloid‐derived suppressor cells and regulatory T cells in a colon cancer model aiming to develop an efficient delivery system for cancer treatment; an in vitro and in vivo study</title><title>Biotechnology and applied biochemistry</title><addtitle>Biotechnol Appl Biochem</addtitle><description>Regulfatory T cells (Tregs) and myeloid‐derived suppressor cells (MDSCs) are common immunosuppressive cells in the tumor microenvironment. These cells, through various mechanisms, inhibit antitumor immune responses and impede effective therapies. Therefore, designing an efficient protocol for inducing immune surveillance in tumors is highly recommended. Recently, nanoliposomes have provided broad‐spectrum and state‐of‐the‐art vehicles to deliver antigens or immune system compartments in immunotherapies. It has been shown that different lipids in the structure of liposomes and various liposomal formulations can affect immune responses in the tumor microenvironment. This study was aimed to evaluate the effects of four different liposomal formulations on MDSCs and Tregs in C26 tumor‐bearing mice. To this end, after preparing liposomes, they were injected into tumor‐inoculated mice and analyzed MDSC and Treg population and functions in spleen and tumor tissues. Results showed that 1,2‐dioleoyl‐3‐trimethylammonium propane (DOTAP)‐containing liposomes reduced MDSC population and activity in the spleen, but not tumor, compared with other groups significantly (p < 0.05 and p < 0.01, respectively). Moreover, DOTAP‐containing liposomes reduced the expression of S100A8 and arginase‐1 genes in splenic MDSCs (p < 0.05). In conclusion, we provided evidence that DOTAP‐containing liposomes contributed to stimulating immune responses and provided a situation to inhibit immunosuppression in the tumor microenvironment.</description><subject>Antigens</subject><subject>Antitumor activity</subject><subject>Arginase</subject><subject>cancer</subject><subject>Cancer therapies</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colorectal cancer</subject><subject>Immune system</subject><subject>Immunoregulation</subject><subject>Immunosuppression</subject><subject>Immunosurveillance</subject><subject>Immunotherapy</subject><subject>In vivo methods and tests</subject><subject>Lipids</subject><subject>liposome</subject><subject>Liposomes</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>MDSC</subject><subject>regulatory T cell</subject><subject>Spleen</subject><subject>Suppressor cells</subject><subject>Tumor microenvironment</subject><subject>Tumors</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1DAQxy1ERZeCxBMgS1y4pLUdZ5OIU1u-KlXiUs6RY08WV_4ItpMqNx6BZ-Q9kHB2t71xsmb0m99_5EHoDSXnlBB20Yv-nLG6eoY2lNekaGrOn6MNaZqq4BUtT9HLGO8JIU3dsBfotOTbtuGk2aC_HyHqndNuhx08YCecN3r00Vth8OCDnYxI2ruIhVMYZmGmXGc6_QAdMAwDyBSxd9guYLxWf379VhD0DArHaRwDxOgDlmDMQRFgtyp9WPDdsa0dFlh6kyVSOAkBW6_AYKHtPsljBXOWj1mwJmqpwaXcNDkme-ISE9h128f5FEAkm6EP60j2zzoFv8_fF7PHMU1qeYVOBmEivD6-Z-j7509311-L229fbq4vbwtZ8rYqaq4ErSjZ9gNnQ_5sQSrRtnKQkjCqWDOQvoS2lEJWeYKCagmhlHHZyC3fkvIMvTt4x-B_ThBTd--n4HJkx2rW8qqsCMvU-wMlg48xwNCNQVsRlo6Sbr1zl6O79c4ZfXsUTr0F9QQ-HjYDxQF40AaW_4q6q8urvfAfnay5Yw</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>Taheri, Ramezan Ali</creator><creator>Bahramifar, Ali</creator><creator>Jaafari, Mahmoud Reza</creator><creator>Fasihi‐Ramandi, Mahdi</creator><creator>Emameh, Reza Zolfaghari</creator><creator>Mohammadian Haftcheshmeh, Saeed</creator><creator>Ebrahimi Nik, Maryam</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-1394-6412</orcidid><orcidid>https://orcid.org/0000-0003-3908-6828</orcidid><orcidid>https://orcid.org/0000-0002-7647-1904</orcidid></search><sort><creationdate>202210</creationdate><title>Designing new nanoliposomal formulations and evaluating their effects on myeloid‐derived suppressor cells and regulatory T cells in a colon cancer model aiming to develop an efficient delivery system for cancer treatment; an in vitro and in vivo study</title><author>Taheri, Ramezan Ali ; Bahramifar, Ali ; Jaafari, Mahmoud Reza ; Fasihi‐Ramandi, Mahdi ; Emameh, Reza Zolfaghari ; Mohammadian Haftcheshmeh, Saeed ; Ebrahimi Nik, Maryam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3495-74da15106bf42fbaba05a99cfcc021d28f0b3e93cac5c341ed9001124c8c64603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antigens</topic><topic>Antitumor activity</topic><topic>Arginase</topic><topic>cancer</topic><topic>Cancer therapies</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colorectal cancer</topic><topic>Immune system</topic><topic>Immunoregulation</topic><topic>Immunosuppression</topic><topic>Immunosurveillance</topic><topic>Immunotherapy</topic><topic>In vivo methods and tests</topic><topic>Lipids</topic><topic>liposome</topic><topic>Liposomes</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>MDSC</topic><topic>regulatory T cell</topic><topic>Spleen</topic><topic>Suppressor cells</topic><topic>Tumor microenvironment</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Taheri, Ramezan Ali</creatorcontrib><creatorcontrib>Bahramifar, Ali</creatorcontrib><creatorcontrib>Jaafari, Mahmoud Reza</creatorcontrib><creatorcontrib>Fasihi‐Ramandi, Mahdi</creatorcontrib><creatorcontrib>Emameh, Reza Zolfaghari</creatorcontrib><creatorcontrib>Mohammadian Haftcheshmeh, Saeed</creatorcontrib><creatorcontrib>Ebrahimi Nik, Maryam</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Taheri, Ramezan Ali</au><au>Bahramifar, Ali</au><au>Jaafari, Mahmoud Reza</au><au>Fasihi‐Ramandi, Mahdi</au><au>Emameh, Reza Zolfaghari</au><au>Mohammadian Haftcheshmeh, Saeed</au><au>Ebrahimi Nik, Maryam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Designing new nanoliposomal formulations and evaluating their effects on myeloid‐derived suppressor cells and regulatory T cells in a colon cancer model aiming to develop an efficient delivery system for cancer treatment; 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These cells, through various mechanisms, inhibit antitumor immune responses and impede effective therapies. Therefore, designing an efficient protocol for inducing immune surveillance in tumors is highly recommended. Recently, nanoliposomes have provided broad‐spectrum and state‐of‐the‐art vehicles to deliver antigens or immune system compartments in immunotherapies. It has been shown that different lipids in the structure of liposomes and various liposomal formulations can affect immune responses in the tumor microenvironment. This study was aimed to evaluate the effects of four different liposomal formulations on MDSCs and Tregs in C26 tumor‐bearing mice. To this end, after preparing liposomes, they were injected into tumor‐inoculated mice and analyzed MDSC and Treg population and functions in spleen and tumor tissues. Results showed that 1,2‐dioleoyl‐3‐trimethylammonium propane (DOTAP)‐containing liposomes reduced MDSC population and activity in the spleen, but not tumor, compared with other groups significantly (p < 0.05 and p < 0.01, respectively). Moreover, DOTAP‐containing liposomes reduced the expression of S100A8 and arginase‐1 genes in splenic MDSCs (p < 0.05). In conclusion, we provided evidence that DOTAP‐containing liposomes contributed to stimulating immune responses and provided a situation to inhibit immunosuppression in the tumor microenvironment.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>34698408</pmid><doi>10.1002/bab.2275</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-1394-6412</orcidid><orcidid>https://orcid.org/0000-0003-3908-6828</orcidid><orcidid>https://orcid.org/0000-0002-7647-1904</orcidid></addata></record> |
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| subjects | Antigens Antitumor activity Arginase cancer Cancer therapies Colon Colon cancer Colorectal cancer Immune system Immunoregulation Immunosuppression Immunosurveillance Immunotherapy In vivo methods and tests Lipids liposome Liposomes Lymphocytes Lymphocytes T MDSC regulatory T cell Spleen Suppressor cells Tumor microenvironment Tumors |
| title | Designing new nanoliposomal formulations and evaluating their effects on myeloid‐derived suppressor cells and regulatory T cells in a colon cancer model aiming to develop an efficient delivery system for cancer treatment; an in vitro and in vivo study |
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