Interaction of MyoD and MyoG with Myoz 2 gene in bovine myoblast differentiation
This study aims to explore the functional role of Myoz2 in myoblast differentiation, and elucidate the potential factors interact with Myoz2 in promoter transcriptional regulation. The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, a...
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| Veröffentlicht in: | Research in veterinary science 2022-12, Vol.152, p.569 |
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| creator | Wei, Dawei Zhang, Jiupan Sayed Haidar Abbas Raza Song, Yaping Jiang, Chao Song, Xiaoyu Wu, Hao Mashael Alhumaidi Alotaibi Albiheyri, Raed Al-Zahrani, Majid Makhlof, Raafat TM Alsaad, Mohammad A Abdelnour, Sameh A Quan, Guobo |
| description | This study aims to explore the functional role of Myoz2 in myoblast differentiation, and elucidate the potential factors interact with Myoz2 in promoter transcriptional regulation. The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, and in individual growth stages and myoblast differentiation stages. Knockdown of Myoz2 inhibited the differentiation of myoblast, and negative effect of MyoD, MyoG, MyH and MEF2A expression on mRNA levels. Subsequently, the promoter region of bovine Myoz2 gene with 1.7 Kb sequence was extracted, and then it was set as eight series of deleted fragments, which were ligated into pGL3-basic to detect core promoter regions of Myoz2 gene in myoblasts and myotubes. Transcription factors MyoD and MyoG were identified as important cis-acting elements in the core promoter region (−159/+1). Also, it was highly conserved in different species based on dual-luciferase analysis and multiple sequence alignment analysis, respectively. Furthermore, a chromatin immunoprecipitation (ChIP) analysis combined with site-directed mutation and siRNA interference and overexpression confirmed that the combination of MyoD and MyoG occurred in region −159/+1, and played an important role in the regulation of bovine Myoz2 gene. These findings explored the regulatory network mechanism of Myoz2 gene during the development of bovine skeletal muscle. |
| doi_str_mv | 10.1016/j.rvsc.2022.09.023 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_2729073645</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2729073645</sourcerecordid><originalsourceid>FETCH-proquest_journals_27290736453</originalsourceid><addsrcrecordid>eNqNzM1qAjEUBeAgFRx_XsDVha4n3tzMj65bqy4EF-4lakYzaGKTqNin1wEfoKvzwTkcxoYCuUBRjGrub2HHCYk4TjiSbLFE5JJSKgrxwRJEmaU5jccd1g2hRsRMiDJhq4WN2qtdNM6Cq2D5cN-g7L7BDO4mHhv9AcFBWw3GwtbdzEvnh9ueVIiwN1WlvbbRqOakz9qVOgU9eGePff5M11_z9OLd71WHuKnd1dtXtaGSJljKIsvl_1ZPfi5F4Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2729073645</pqid></control><display><type>article</type><title>Interaction of MyoD and MyoG with Myoz 2 gene in bovine myoblast differentiation</title><source>Elsevier ScienceDirect Journals</source><creator>Wei, Dawei ; Zhang, Jiupan ; Sayed Haidar Abbas Raza ; Song, Yaping ; Jiang, Chao ; Song, Xiaoyu ; Wu, Hao ; Mashael Alhumaidi Alotaibi ; Albiheyri, Raed ; Al-Zahrani, Majid ; Makhlof, Raafat TM ; Alsaad, Mohammad A ; Abdelnour, Sameh A ; Quan, Guobo</creator><creatorcontrib>Wei, Dawei ; Zhang, Jiupan ; Sayed Haidar Abbas Raza ; Song, Yaping ; Jiang, Chao ; Song, Xiaoyu ; Wu, Hao ; Mashael Alhumaidi Alotaibi ; Albiheyri, Raed ; Al-Zahrani, Majid ; Makhlof, Raafat TM ; Alsaad, Mohammad A ; Abdelnour, Sameh A ; Quan, Guobo</creatorcontrib><description>This study aims to explore the functional role of Myoz2 in myoblast differentiation, and elucidate the potential factors interact with Myoz2 in promoter transcriptional regulation. The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, and in individual growth stages and myoblast differentiation stages. Knockdown of Myoz2 inhibited the differentiation of myoblast, and negative effect of MyoD, MyoG, MyH and MEF2A expression on mRNA levels. Subsequently, the promoter region of bovine Myoz2 gene with 1.7 Kb sequence was extracted, and then it was set as eight series of deleted fragments, which were ligated into pGL3-basic to detect core promoter regions of Myoz2 gene in myoblasts and myotubes. Transcription factors MyoD and MyoG were identified as important cis-acting elements in the core promoter region (−159/+1). Also, it was highly conserved in different species based on dual-luciferase analysis and multiple sequence alignment analysis, respectively. Furthermore, a chromatin immunoprecipitation (ChIP) analysis combined with site-directed mutation and siRNA interference and overexpression confirmed that the combination of MyoD and MyoG occurred in region −159/+1, and played an important role in the regulation of bovine Myoz2 gene. These findings explored the regulatory network mechanism of Myoz2 gene during the development of bovine skeletal muscle.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2022.09.023</identifier><language>eng</language><publisher>Oxford: Elsevier Limited</publisher><subject>Beef ; Binding sites ; Cattle ; Chromatin ; Conserved sequence ; Differentiation ; DNA methylation ; Gene expression ; Gene regulation ; Genes ; Immunoprecipitation ; Kinases ; Laboratory animals ; Meat quality ; Muscles ; Musculoskeletal system ; Mutation ; Myoblasts ; MyoD protein ; Myotubes ; Nucleotide sequence ; Plasmids ; Proteins ; siRNA ; Skeletal muscle ; Transcription factors ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2022-12, Vol.152, p.569</ispartof><rights>2022. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Wei, Dawei</creatorcontrib><creatorcontrib>Zhang, Jiupan</creatorcontrib><creatorcontrib>Sayed Haidar Abbas Raza</creatorcontrib><creatorcontrib>Song, Yaping</creatorcontrib><creatorcontrib>Jiang, Chao</creatorcontrib><creatorcontrib>Song, Xiaoyu</creatorcontrib><creatorcontrib>Wu, Hao</creatorcontrib><creatorcontrib>Mashael Alhumaidi Alotaibi</creatorcontrib><creatorcontrib>Albiheyri, Raed</creatorcontrib><creatorcontrib>Al-Zahrani, Majid</creatorcontrib><creatorcontrib>Makhlof, Raafat TM</creatorcontrib><creatorcontrib>Alsaad, Mohammad A</creatorcontrib><creatorcontrib>Abdelnour, Sameh A</creatorcontrib><creatorcontrib>Quan, Guobo</creatorcontrib><title>Interaction of MyoD and MyoG with Myoz 2 gene in bovine myoblast differentiation</title><title>Research in veterinary science</title><description>This study aims to explore the functional role of Myoz2 in myoblast differentiation, and elucidate the potential factors interact with Myoz2 in promoter transcriptional regulation. The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, and in individual growth stages and myoblast differentiation stages. Knockdown of Myoz2 inhibited the differentiation of myoblast, and negative effect of MyoD, MyoG, MyH and MEF2A expression on mRNA levels. Subsequently, the promoter region of bovine Myoz2 gene with 1.7 Kb sequence was extracted, and then it was set as eight series of deleted fragments, which were ligated into pGL3-basic to detect core promoter regions of Myoz2 gene in myoblasts and myotubes. Transcription factors MyoD and MyoG were identified as important cis-acting elements in the core promoter region (−159/+1). Also, it was highly conserved in different species based on dual-luciferase analysis and multiple sequence alignment analysis, respectively. Furthermore, a chromatin immunoprecipitation (ChIP) analysis combined with site-directed mutation and siRNA interference and overexpression confirmed that the combination of MyoD and MyoG occurred in region −159/+1, and played an important role in the regulation of bovine Myoz2 gene. These findings explored the regulatory network mechanism of Myoz2 gene during the development of bovine skeletal muscle.</description><subject>Beef</subject><subject>Binding sites</subject><subject>Cattle</subject><subject>Chromatin</subject><subject>Conserved sequence</subject><subject>Differentiation</subject><subject>DNA methylation</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Immunoprecipitation</subject><subject>Kinases</subject><subject>Laboratory animals</subject><subject>Meat quality</subject><subject>Muscles</subject><subject>Musculoskeletal system</subject><subject>Mutation</subject><subject>Myoblasts</subject><subject>MyoD protein</subject><subject>Myotubes</subject><subject>Nucleotide sequence</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>siRNA</subject><subject>Skeletal muscle</subject><subject>Transcription factors</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqNzM1qAjEUBeAgFRx_XsDVha4n3tzMj65bqy4EF-4lakYzaGKTqNin1wEfoKvzwTkcxoYCuUBRjGrub2HHCYk4TjiSbLFE5JJSKgrxwRJEmaU5jccd1g2hRsRMiDJhq4WN2qtdNM6Cq2D5cN-g7L7BDO4mHhv9AcFBWw3GwtbdzEvnh9ueVIiwN1WlvbbRqOakz9qVOgU9eGePff5M11_z9OLd71WHuKnd1dtXtaGSJljKIsvl_1ZPfi5F4Q</recordid><startdate>20221220</startdate><enddate>20221220</enddate><creator>Wei, Dawei</creator><creator>Zhang, Jiupan</creator><creator>Sayed Haidar Abbas Raza</creator><creator>Song, Yaping</creator><creator>Jiang, Chao</creator><creator>Song, Xiaoyu</creator><creator>Wu, Hao</creator><creator>Mashael Alhumaidi Alotaibi</creator><creator>Albiheyri, Raed</creator><creator>Al-Zahrani, Majid</creator><creator>Makhlof, Raafat TM</creator><creator>Alsaad, Mohammad A</creator><creator>Abdelnour, Sameh A</creator><creator>Quan, Guobo</creator><general>Elsevier Limited</general><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20221220</creationdate><title>Interaction of MyoD and MyoG with Myoz 2 gene in bovine myoblast differentiation</title><author>Wei, Dawei ; 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The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, and in individual growth stages and myoblast differentiation stages. Knockdown of Myoz2 inhibited the differentiation of myoblast, and negative effect of MyoD, MyoG, MyH and MEF2A expression on mRNA levels. Subsequently, the promoter region of bovine Myoz2 gene with 1.7 Kb sequence was extracted, and then it was set as eight series of deleted fragments, which were ligated into pGL3-basic to detect core promoter regions of Myoz2 gene in myoblasts and myotubes. Transcription factors MyoD and MyoG were identified as important cis-acting elements in the core promoter region (−159/+1). Also, it was highly conserved in different species based on dual-luciferase analysis and multiple sequence alignment analysis, respectively. Furthermore, a chromatin immunoprecipitation (ChIP) analysis combined with site-directed mutation and siRNA interference and overexpression confirmed that the combination of MyoD and MyoG occurred in region −159/+1, and played an important role in the regulation of bovine Myoz2 gene. These findings explored the regulatory network mechanism of Myoz2 gene during the development of bovine skeletal muscle.</abstract><cop>Oxford</cop><pub>Elsevier Limited</pub><doi>10.1016/j.rvsc.2022.09.023</doi></addata></record> |
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| subjects | Beef Binding sites Cattle Chromatin Conserved sequence Differentiation DNA methylation Gene expression Gene regulation Genes Immunoprecipitation Kinases Laboratory animals Meat quality Muscles Musculoskeletal system Mutation Myoblasts MyoD protein Myotubes Nucleotide sequence Plasmids Proteins siRNA Skeletal muscle Transcription factors Veterinary medicine |
| title | Interaction of MyoD and MyoG with Myoz 2 gene in bovine myoblast differentiation |
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