Improved quality of Kambing Kacang sexing frozen semen with the additon of green tea extract
Objectve: The objectve of this study was to determine the efect of adding various doses of green tea extract to the semen of Kacang goats during the sexing process on motlity, viability, membrane integrity, malondialdehide (MDA), and deoxyribonucleic acid (DNA) fragmentaton. Materials and Methods: I...
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| Veröffentlicht in: | Journal of advanced veterinary and animal research 2022-09, Vol.9 (3), p.412-418 |
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| container_title | Journal of advanced veterinary and animal research |
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| creator | Suprayogi, Tri Wahyu Susilowat, Suherni Hernawat, Tatk Hafdha, Farah Ghifara Wening, Citra Ayu Purnawan, Ahmad Budi |
| description | Objectve: The objectve of this study was to determine the efect of adding various doses of green tea extract to the semen of Kacang goats during the sexing process on motlity, viability, membrane integrity, malondialdehide (MDA), and deoxyribonucleic acid (DNA) fragmentaton. Materials and Methods: It started with the containment of the semen of the Kacang goat, followed by macroscopic and microscopic examinatons. If the semen was considered viable, a diluter that had been added with various doses of green tea extract would be added to the semen. Afer that, sexing was carried out using the percoll gradient density medium. Next, the sexed semen was cryopreserved in liquid nitrogen. Then, an examinaton of motlity, viability, membrane integrity, MDA, and DNA fragmentaton was conducted. Result: There was a signifcant diference between the control and treatment (p = 0.05). The highest result was obtained in the treatment of adding 0.05 mg of green tea extract/100 ml of Andromed(r). Conclusion: The additon of green tea extract can improve the quality of the sexed semen of the Kacang goat afer it has been cryopreserved. |
| doi_str_mv | 10.5455/javar.2022.i609 |
| format | Article |
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Materials and Methods: It started with the containment of the semen of the Kacang goat, followed by macroscopic and microscopic examinatons. If the semen was considered viable, a diluter that had been added with various doses of green tea extract would be added to the semen. Afer that, sexing was carried out using the percoll gradient density medium. Next, the sexed semen was cryopreserved in liquid nitrogen. Then, an examinaton of motlity, viability, membrane integrity, MDA, and DNA fragmentaton was conducted. Result: There was a signifcant diference between the control and treatment (p = 0.05). The highest result was obtained in the treatment of adding 0.05 mg of green tea extract/100 ml of Andromed(r). Conclusion: The additon of green tea extract can improve the quality of the sexed semen of the Kacang goat afer it has been cryopreserved.</description><identifier>EISSN: 2311-7710</identifier><identifier>DOI: 10.5455/javar.2022.i609</identifier><language>eng</language><publisher>Mymensingh: Network for the Veterinarians of Bangladesh Bangladesh Agricultural Universityת Faculty of Veterinary Science</publisher><subject>Antioxidants ; Artificial insemination ; Cold ; Cryopreservation ; Deoxyribonucleic acid ; DNA ; Ethics ; Flavonoids ; Goats ; Green tea ; Integrity ; Laboratories ; Liquid nitrogen ; Livestock ; Membranes ; Motility ; Plasma ; Polyphenols ; Semen ; Sexing ; Sperm ; Tea</subject><ispartof>Journal of advanced veterinary and animal research, 2022-09, Vol.9 (3), p.412-418</ispartof><rights>2022. This work is published under https://creativecommons.org/licenses/by/2.0/ (the“License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,27905,27906</link.rule.ids></links><search><creatorcontrib>Suprayogi, Tri Wahyu</creatorcontrib><creatorcontrib>Susilowat, Suherni</creatorcontrib><creatorcontrib>Hernawat, Tatk</creatorcontrib><creatorcontrib>Hafdha, Farah Ghifara</creatorcontrib><creatorcontrib>Wening, Citra Ayu</creatorcontrib><creatorcontrib>Purnawan, Ahmad Budi</creatorcontrib><title>Improved quality of Kambing Kacang sexing frozen semen with the additon of green tea extract</title><title>Journal of advanced veterinary and animal research</title><description>Objectve: The objectve of this study was to determine the efect of adding various doses of green tea extract to the semen of Kacang goats during the sexing process on motlity, viability, membrane integrity, malondialdehide (MDA), and deoxyribonucleic acid (DNA) fragmentaton. Materials and Methods: It started with the containment of the semen of the Kacang goat, followed by macroscopic and microscopic examinatons. If the semen was considered viable, a diluter that had been added with various doses of green tea extract would be added to the semen. Afer that, sexing was carried out using the percoll gradient density medium. Next, the sexed semen was cryopreserved in liquid nitrogen. Then, an examinaton of motlity, viability, membrane integrity, MDA, and DNA fragmentaton was conducted. Result: There was a signifcant diference between the control and treatment (p = 0.05). The highest result was obtained in the treatment of adding 0.05 mg of green tea extract/100 ml of Andromed(r). 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Susilowat, Suherni ; Hernawat, Tatk ; Hafdha, Farah Ghifara ; Wening, Citra Ayu ; Purnawan, Ahmad Budi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_27253494733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antioxidants</topic><topic>Artificial insemination</topic><topic>Cold</topic><topic>Cryopreservation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Ethics</topic><topic>Flavonoids</topic><topic>Goats</topic><topic>Green tea</topic><topic>Integrity</topic><topic>Laboratories</topic><topic>Liquid nitrogen</topic><topic>Livestock</topic><topic>Membranes</topic><topic>Motility</topic><topic>Plasma</topic><topic>Polyphenols</topic><topic>Semen</topic><topic>Sexing</topic><topic>Sperm</topic><topic>Tea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suprayogi, Tri Wahyu</creatorcontrib><creatorcontrib>Susilowat, Suherni</creatorcontrib><creatorcontrib>Hernawat, Tatk</creatorcontrib><creatorcontrib>Hafdha, Farah Ghifara</creatorcontrib><creatorcontrib>Wening, Citra Ayu</creatorcontrib><creatorcontrib>Purnawan, Ahmad Budi</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of advanced veterinary and animal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suprayogi, Tri Wahyu</au><au>Susilowat, Suherni</au><au>Hernawat, Tatk</au><au>Hafdha, Farah Ghifara</au><au>Wening, Citra Ayu</au><au>Purnawan, Ahmad Budi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved quality of Kambing Kacang sexing frozen semen with the additon of green tea extract</atitle><jtitle>Journal of advanced veterinary and animal research</jtitle><date>2022-09-01</date><risdate>2022</risdate><volume>9</volume><issue>3</issue><spage>412</spage><epage>418</epage><pages>412-418</pages><eissn>2311-7710</eissn><abstract>Objectve: The objectve of this study was to determine the efect of adding various doses of green tea extract to the semen of Kacang goats during the sexing process on motlity, viability, membrane integrity, malondialdehide (MDA), and deoxyribonucleic acid (DNA) fragmentaton. Materials and Methods: It started with the containment of the semen of the Kacang goat, followed by macroscopic and microscopic examinatons. If the semen was considered viable, a diluter that had been added with various doses of green tea extract would be added to the semen. Afer that, sexing was carried out using the percoll gradient density medium. Next, the sexed semen was cryopreserved in liquid nitrogen. Then, an examinaton of motlity, viability, membrane integrity, MDA, and DNA fragmentaton was conducted. Result: There was a signifcant diference between the control and treatment (p = 0.05). The highest result was obtained in the treatment of adding 0.05 mg of green tea extract/100 ml of Andromed(r). 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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Antioxidants Artificial insemination Cold Cryopreservation Deoxyribonucleic acid DNA Ethics Flavonoids Goats Green tea Integrity Laboratories Liquid nitrogen Livestock Membranes Motility Plasma Polyphenols Semen Sexing Sperm Tea |
| title | Improved quality of Kambing Kacang sexing frozen semen with the additon of green tea extract |
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