Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags
Rapid, direct and sensitive detection of foodborne bacteria in complex samples is still challenging. Here, we reported a universal surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for highly sensitive detection of foodborne bacteria in food and environmental samples using whea...
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| Veröffentlicht in: | RSC advances 2022-09, Vol.12 (42), p.27344-27354 |
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| container_issue | 42 |
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| container_title | RSC advances |
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| creator | Tu, Zhijie Cheng, Siyun Dong, Hao Wang, Wenqi Yang, Xingsheng Gu, Bing Wang, Shengqi Wang, Chongwen |
| description | Rapid, direct and sensitive detection of foodborne bacteria in complex samples is still challenging. Here, we reported a universal surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin (WGA)-modified Fe
3
O
4
@Au (Au@MNP-WGA) nanotags. The Au@MNP-WGA tag with numerous intraparticle hotspots was integrated into the LFA system for the first time, which can not only greatly improve the detection sensitivity through the dual amplification effect of magnetic enrichment and SERS enhancement but also achieve the broad-spectrum capture of multiple bacteria. In addition, monoclonal antibodies were separately immobilized onto the test line of different LFA strips to ensure the specific detection of different target pathogens. With this strategy, the proposed assay can achieve the universal and highly sensitive determination of three common foodborne bacteria, namely,
Listeria monocytogenes
,
Campylobacter jejuni
, and
Staphylococcus aureus
, with low detection limit (10 cells mL
−1
), short testing time ( |
| doi_str_mv | 10.1039/d2ra04735g |
| format | Article |
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3
O
4
@Au (Au@MNP-WGA) nanotags. The Au@MNP-WGA tag with numerous intraparticle hotspots was integrated into the LFA system for the first time, which can not only greatly improve the detection sensitivity through the dual amplification effect of magnetic enrichment and SERS enhancement but also achieve the broad-spectrum capture of multiple bacteria. In addition, monoclonal antibodies were separately immobilized onto the test line of different LFA strips to ensure the specific detection of different target pathogens. With this strategy, the proposed assay can achieve the universal and highly sensitive determination of three common foodborne bacteria, namely,
Listeria monocytogenes
,
Campylobacter jejuni
, and
Staphylococcus aureus
, with low detection limit (10 cells mL
−1
), short testing time (<35 min), and high reproducibility (RSD < 8.14%). Given its good stability and accuracy in complex samples, the Au@MNP-WGA-based SERS-LFA has great potential to be a powerful tool for the universal and on-site detection of different foodborne pathogens.
A universal SERS-based lateral flow assay was proposed for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin-modified Fe
3
O
4
@Au nanotags.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d2ra04735g</identifier><identifier>PMID: 36276007</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Assaying ; Bacteria ; Food irradiation ; Iron oxides ; Monoclonal antibodies ; Pathogens ; Raman spectra ; Target detection ; Testing time ; Wheat germ</subject><ispartof>RSC advances, 2022-09, Vol.12 (42), p.27344-27354</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-333b965dc9bd26696acc896fe5ab32bab949bb656b157378c36540b134d915f33</citedby><cites>FETCH-LOGICAL-c373t-333b965dc9bd26696acc896fe5ab32bab949bb656b157378c36540b134d915f33</cites><orcidid>0000-0002-9627-107X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36276007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tu, Zhijie</creatorcontrib><creatorcontrib>Cheng, Siyun</creatorcontrib><creatorcontrib>Dong, Hao</creatorcontrib><creatorcontrib>Wang, Wenqi</creatorcontrib><creatorcontrib>Yang, Xingsheng</creatorcontrib><creatorcontrib>Gu, Bing</creatorcontrib><creatorcontrib>Wang, Shengqi</creatorcontrib><creatorcontrib>Wang, Chongwen</creatorcontrib><title>Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>Rapid, direct and sensitive detection of foodborne bacteria in complex samples is still challenging. Here, we reported a universal surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin (WGA)-modified Fe
3
O
4
@Au (Au@MNP-WGA) nanotags. The Au@MNP-WGA tag with numerous intraparticle hotspots was integrated into the LFA system for the first time, which can not only greatly improve the detection sensitivity through the dual amplification effect of magnetic enrichment and SERS enhancement but also achieve the broad-spectrum capture of multiple bacteria. In addition, monoclonal antibodies were separately immobilized onto the test line of different LFA strips to ensure the specific detection of different target pathogens. With this strategy, the proposed assay can achieve the universal and highly sensitive determination of three common foodborne bacteria, namely,
Listeria monocytogenes
,
Campylobacter jejuni
, and
Staphylococcus aureus
, with low detection limit (10 cells mL
−1
), short testing time (<35 min), and high reproducibility (RSD < 8.14%). Given its good stability and accuracy in complex samples, the Au@MNP-WGA-based SERS-LFA has great potential to be a powerful tool for the universal and on-site detection of different foodborne pathogens.
A universal SERS-based lateral flow assay was proposed for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin-modified Fe
3
O
4
@Au nanotags.</description><subject>Assaying</subject><subject>Bacteria</subject><subject>Food irradiation</subject><subject>Iron oxides</subject><subject>Monoclonal antibodies</subject><subject>Pathogens</subject><subject>Raman spectra</subject><subject>Target detection</subject><subject>Testing time</subject><subject>Wheat germ</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpd0UtrFTEUB_BBFFtqN-6VA25EGM1jkrlZllqrUBBaux5OHjOmzCTXJNNyP4rf1tRbq5hNHueXk8C_aV5S8p4Srj5YlpB0PRfTk-aQkU62jEj19J_1QXOc8w2pQwrKJH3eHHDJeklIf9j8vA7-1qWMM2CwsM4lYXYh-1KPwbriTPExQBxhjNHqmIIDjaa45BFqAWHGuqn3xzneAeaMO8gl-S3oHazZhwnuvjssMLm0AE7TvBYffGiXaP3onYUFp-CKN3B1dnkFAUMsOOUXzbMR5-yOH-aj5vrT2bfTz-3F1_MvpycXreE9Ly3nXCsprFHaMimVRGM2So5OoOZMo1ad0loKqanoeb8xXIqOaMo7q6gYOT9q3u77blP8sbpchsVn4-YZg4trHljPNrQTlPaVvvmP3sQ1hfq7e0VFfUFtqnq3VybFnJMbh23yC6bdQMlwn9nwkV2e_M7svOLXDy1XvTj7SP8kVMGrPUjZPFb_hs5_Afd8nQo</recordid><startdate>20220922</startdate><enddate>20220922</enddate><creator>Tu, Zhijie</creator><creator>Cheng, Siyun</creator><creator>Dong, Hao</creator><creator>Wang, Wenqi</creator><creator>Yang, Xingsheng</creator><creator>Gu, Bing</creator><creator>Wang, Shengqi</creator><creator>Wang, Chongwen</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9627-107X</orcidid></search><sort><creationdate>20220922</creationdate><title>Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags</title><author>Tu, Zhijie ; Cheng, Siyun ; Dong, Hao ; Wang, Wenqi ; Yang, Xingsheng ; Gu, Bing ; Wang, Shengqi ; Wang, Chongwen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-333b965dc9bd26696acc896fe5ab32bab949bb656b157378c36540b134d915f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Assaying</topic><topic>Bacteria</topic><topic>Food irradiation</topic><topic>Iron oxides</topic><topic>Monoclonal antibodies</topic><topic>Pathogens</topic><topic>Raman spectra</topic><topic>Target detection</topic><topic>Testing time</topic><topic>Wheat germ</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tu, Zhijie</creatorcontrib><creatorcontrib>Cheng, Siyun</creatorcontrib><creatorcontrib>Dong, Hao</creatorcontrib><creatorcontrib>Wang, Wenqi</creatorcontrib><creatorcontrib>Yang, Xingsheng</creatorcontrib><creatorcontrib>Gu, Bing</creatorcontrib><creatorcontrib>Wang, Shengqi</creatorcontrib><creatorcontrib>Wang, Chongwen</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tu, Zhijie</au><au>Cheng, Siyun</au><au>Dong, Hao</au><au>Wang, Wenqi</au><au>Yang, Xingsheng</au><au>Gu, Bing</au><au>Wang, Shengqi</au><au>Wang, Chongwen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2022-09-22</date><risdate>2022</risdate><volume>12</volume><issue>42</issue><spage>27344</spage><epage>27354</epage><pages>27344-27354</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Rapid, direct and sensitive detection of foodborne bacteria in complex samples is still challenging. Here, we reported a universal surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin (WGA)-modified Fe
3
O
4
@Au (Au@MNP-WGA) nanotags. The Au@MNP-WGA tag with numerous intraparticle hotspots was integrated into the LFA system for the first time, which can not only greatly improve the detection sensitivity through the dual amplification effect of magnetic enrichment and SERS enhancement but also achieve the broad-spectrum capture of multiple bacteria. In addition, monoclonal antibodies were separately immobilized onto the test line of different LFA strips to ensure the specific detection of different target pathogens. With this strategy, the proposed assay can achieve the universal and highly sensitive determination of three common foodborne bacteria, namely,
Listeria monocytogenes
,
Campylobacter jejuni
, and
Staphylococcus aureus
, with low detection limit (10 cells mL
−1
), short testing time (<35 min), and high reproducibility (RSD < 8.14%). Given its good stability and accuracy in complex samples, the Au@MNP-WGA-based SERS-LFA has great potential to be a powerful tool for the universal and on-site detection of different foodborne pathogens.
A universal SERS-based lateral flow assay was proposed for highly sensitive detection of foodborne bacteria in food and environmental samples using wheat germ agglutinin-modified Fe
3
O
4
@Au nanotags.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>36276007</pmid><doi>10.1039/d2ra04735g</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-9627-107X</orcidid><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Assaying Bacteria Food irradiation Iron oxides Monoclonal antibodies Pathogens Raman spectra Target detection Testing time Wheat germ |
| title | Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags |
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