Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters
Abstract Objective: to verify the stability of vancomycin hydrochloride in antimicrobial seal solutions with and without association of heparin sodium according to temperature and association time. Method: an experimental study designed for the analysis of hydrogenionic potential and concentration b...
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| creator | Barros, Daniele Porto Onofre, Priscilla Sete de Carvalho Fonseca, Fernando Luiz Affonso Rosa, Paulo César Pires Pedreira, Mavilde da Luz Gonçalves Peterlini, Maria Angélica Sorgini |
| description | Abstract Objective: to verify the stability of vancomycin hydrochloride in antimicrobial seal solutions with and without association of heparin sodium according to temperature and association time. Method: an experimental study designed for the analysis of hydrogenionic potential and concentration by means of high-efficiency liquid chromatography of vancomycin hydrochloride (n=06) and vancomycin hydrochloride and heparin sodium (n=06). The solutions studied were submitted to absence of light, as well as to 22°C and 37°C. Analyses in triplicate (n=192) were performed at the initial moment (T0) and three (T3), eight (T8) and 24 hours (T24) after preparation. The data were submitted to analysis of variance (p≤0.05). Results: concentration of the antimicrobial at 22°C presented a reduction (T0-T8) and a subsequent increase (T24); hydrogenionic potential decreased significantly over time. At 37°C, the concentration increased up to T3 and decreased at T24, with a reduction of hydrogenionic potential up to 24 hours. Concentration of the vancomycin hydrochloride and heparin sodium solutions varied with a reduction at 22°C, accompanied by increased hydrogenionic potential. Precipitate formation was observed by visual inspection of the vancomycin hydrochloride-heparin sodium association (T3). Conclusion: pharmacological stability of vancomycin hydrochloride (5 mg/mL) and physical incompatibility with heparin sodium (100 IU/mL) were evidenced after three hours of association in the antimicrobial seal solutions studied.
Resumo Objetivo: verificar a estabilidade do cloridrato de vancomicina em soluções de selo antimicrobiano sem e com associação de heparina sódica segundo a temperatura e tempo de associação. Método: estudo experimental delineado para análise de potencial hidrogeniônico e concentração por cromatografia líquida de alta eficiência de soluções de cloridrato de vancomicina (n=06) e cloridrato de vancomicina e heparina sódica (n=06). Submeteram-se as soluções estudadas à ausência de luz, 22°C e 37°C. Análises em triplicadas (n=192) ocorreram no momento inicial (T0), três (T3), oito (T8) e 24 horas (T24) após preparo. Os dados foram submetidos à análise de variância (p≤0,05). Resultados: a concentração do antimicrobiano a 22°C apresentou redução (T0-T8) e posterior elevação (T24); o potencial hidrogeniônico diminuiu significativamente ao longo do tempo. Em 37°C a concentração aumentou em até T3 e reduziu em T24, com redução de potencial hidrogeniônico até 24 |
| doi_str_mv | 10.1590/1518.8345.5869.3621 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2718755341</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2718755341</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1171-eac0b1ec894addc472eb83b6c87d35c080b480512729ea55ec2dde8fd9a4f4383</originalsourceid><addsrcrecordid>eNpNkE9LxDAQxYMouK5-Ai8Fz62ZptmmR1n8Bwse1HNIkymbpW3WJF3ot7dhPXiZN7x5zDA_Qu6BFsAb-ggcRCFYxQsuNk3BNiVckFVy8-Re_uuvyU0IB0rLBiisiPuMqrW9jXPmuuykRu2GWdsx28_GO73vnbcGMxyOvZvRZMtEjdEOVnvXWtVnAVNx_RStG0NaonGMfjFtkhOObgqZVnGPEX24JVed6gPe_emafL88f23f8t3H6_v2aZdrgBpyVJq2gFo0lTJGV3WJrWDtRovaMK6poG0lKIeyLhtUnKMujUHRmUZVXcUEW5OH896jdz8ThigPbvLjclKWNYiac1bBkmLn1PJNCB47efR2UH6WQGUiKxM3mbjJRFYmsuwX3Idu7Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2718755341</pqid></control><display><type>article</type><title>Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>PubMed Central</source><creator>Barros, Daniele Porto ; Onofre, Priscilla Sete de Carvalho ; Fonseca, Fernando Luiz Affonso ; Rosa, Paulo César Pires ; Pedreira, Mavilde da Luz Gonçalves ; Peterlini, Maria Angélica Sorgini</creator><creatorcontrib>Barros, Daniele Porto ; Onofre, Priscilla Sete de Carvalho ; Fonseca, Fernando Luiz Affonso ; Rosa, Paulo César Pires ; Pedreira, Mavilde da Luz Gonçalves ; Peterlini, Maria Angélica Sorgini</creatorcontrib><description>Abstract Objective: to verify the stability of vancomycin hydrochloride in antimicrobial seal solutions with and without association of heparin sodium according to temperature and association time. Method: an experimental study designed for the analysis of hydrogenionic potential and concentration by means of high-efficiency liquid chromatography of vancomycin hydrochloride (n=06) and vancomycin hydrochloride and heparin sodium (n=06). The solutions studied were submitted to absence of light, as well as to 22°C and 37°C. Analyses in triplicate (n=192) were performed at the initial moment (T0) and three (T3), eight (T8) and 24 hours (T24) after preparation. The data were submitted to analysis of variance (p≤0.05). Results: concentration of the antimicrobial at 22°C presented a reduction (T0-T8) and a subsequent increase (T24); hydrogenionic potential decreased significantly over time. At 37°C, the concentration increased up to T3 and decreased at T24, with a reduction of hydrogenionic potential up to 24 hours. Concentration of the vancomycin hydrochloride and heparin sodium solutions varied with a reduction at 22°C, accompanied by increased hydrogenionic potential. Precipitate formation was observed by visual inspection of the vancomycin hydrochloride-heparin sodium association (T3). Conclusion: pharmacological stability of vancomycin hydrochloride (5 mg/mL) and physical incompatibility with heparin sodium (100 IU/mL) were evidenced after three hours of association in the antimicrobial seal solutions studied.
Resumo Objetivo: verificar a estabilidade do cloridrato de vancomicina em soluções de selo antimicrobiano sem e com associação de heparina sódica segundo a temperatura e tempo de associação. Método: estudo experimental delineado para análise de potencial hidrogeniônico e concentração por cromatografia líquida de alta eficiência de soluções de cloridrato de vancomicina (n=06) e cloridrato de vancomicina e heparina sódica (n=06). Submeteram-se as soluções estudadas à ausência de luz, 22°C e 37°C. Análises em triplicadas (n=192) ocorreram no momento inicial (T0), três (T3), oito (T8) e 24 horas (T24) após preparo. Os dados foram submetidos à análise de variância (p≤0,05). Resultados: a concentração do antimicrobiano a 22°C apresentou redução (T0-T8) e posterior elevação (T24); o potencial hidrogeniônico diminuiu significativamente ao longo do tempo. Em 37°C a concentração aumentou em até T3 e reduziu em T24, com redução de potencial hidrogeniônico até 24 horas. A concentração das soluções de cloridrato de vancomicina e heparina sódica apresentaram variação com redução a 22°C acompanhada de aumento de potencial hidrogeniônico. Observou-se formação de precipitado por inspeção visual da associação cloridrato de vancomicina e heparina sódica (T3). Conclusão: evidenciou-se estabilidade farmacológica do cloridrato de vancomicina (5 mg/mL) e incompatibilidade física com heparina sódica (100 UI/mL) após três horas de associação nas soluções de selo antimicrobiano estudadas.
Resumen Objetivo: verificar la estabilidad del clorhidrato de vancomicina en soluciones de sellado antimicrobiano solo y combinado con heparina sódica según la temperatura y el tiempo de combinación. Método: estudio experimental diseñado para analizar el potencial de hidrógeno y la concentración por cromatografía líquida de alta resolución de soluciones de clorhidrato de vancomicina (n=06) y de clorhidrato de vancomicina y heparina sódica (n=06). Las soluciones estudiadas fueron sometidas a ausencia de luz, 22°C y 37°C. Se realizaron análisis por triplicado (n=192) en el momento inicial (T0), a las tres (T3), ocho (T8) y 24 horas (T24) después de la preparación. Los datos fueron sometidos a análisis de varianza (p≤0,05). Resultados: la concentración de antimicrobiano a 22°C mostró una reducción (T0-T8) y un posterior aumento (T24); el potencial de hidrógeno disminuyó significativamente con el tiempo. A 37°C, la concentración aumentó hasta T3 y disminuyó en T24, el potencial de hidrógeno disminuyó hasta las 24 horas. La concentración de las soluciones de clorhidrato de vancomicina y heparina sódica mostró variación con la reducción a 22°C acompañada de un aumento del potencial de hidrógeno. Mediante inspección visual se observó la formación de un precipitado al combinar clorhidrato de vancomicina y heparina sódica (T3). Conclusión: el clorhidrato de vancomicina (5 mg/ml) presentó evidencia de estabilidad farmacológica e incompatibilidad física con la heparina sódica (100 UI/ml) después de las tres horas de haberse realizado la combinación en las soluciones de sellado antimicrobiano estudiadas.</description><identifier>ISSN: 1518-8345</identifier><identifier>ISSN: 0104-1169</identifier><identifier>EISSN: 1518-8345</identifier><identifier>DOI: 10.1590/1518.8345.5869.3621</identifier><language>eng</language><publisher>Sao Paulo: Universidade de São Paulo-USP, Escola de Enfermagem de Ribeirão Preto - USP</publisher><subject>Antibiotics ; Anticoagulants ; Antimicrobial agents ; Catheters ; Disease prevention ; Intravenous therapy ; Sodium</subject><ispartof>Revista latino-americana de enfermagem, 2022, Vol.30</ispartof><rights>2022. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1171-eac0b1ec894addc472eb83b6c87d35c080b480512729ea55ec2dde8fd9a4f4383</cites><orcidid>0000-0003-1769-4662 ; 0000-0003-3665-8878 ; 0000-0002-4388-3793 ; 0000-0002-8830-0993 ; 0000-0002-9246-2354 ; 0000-0003-1223-1589</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,4024,27923,27924,27925</link.rule.ids></links><search><creatorcontrib>Barros, Daniele Porto</creatorcontrib><creatorcontrib>Onofre, Priscilla Sete de Carvalho</creatorcontrib><creatorcontrib>Fonseca, Fernando Luiz Affonso</creatorcontrib><creatorcontrib>Rosa, Paulo César Pires</creatorcontrib><creatorcontrib>Pedreira, Mavilde da Luz Gonçalves</creatorcontrib><creatorcontrib>Peterlini, Maria Angélica Sorgini</creatorcontrib><title>Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters</title><title>Revista latino-americana de enfermagem</title><description>Abstract Objective: to verify the stability of vancomycin hydrochloride in antimicrobial seal solutions with and without association of heparin sodium according to temperature and association time. Method: an experimental study designed for the analysis of hydrogenionic potential and concentration by means of high-efficiency liquid chromatography of vancomycin hydrochloride (n=06) and vancomycin hydrochloride and heparin sodium (n=06). The solutions studied were submitted to absence of light, as well as to 22°C and 37°C. Analyses in triplicate (n=192) were performed at the initial moment (T0) and three (T3), eight (T8) and 24 hours (T24) after preparation. The data were submitted to analysis of variance (p≤0.05). Results: concentration of the antimicrobial at 22°C presented a reduction (T0-T8) and a subsequent increase (T24); hydrogenionic potential decreased significantly over time. At 37°C, the concentration increased up to T3 and decreased at T24, with a reduction of hydrogenionic potential up to 24 hours. Concentration of the vancomycin hydrochloride and heparin sodium solutions varied with a reduction at 22°C, accompanied by increased hydrogenionic potential. Precipitate formation was observed by visual inspection of the vancomycin hydrochloride-heparin sodium association (T3). Conclusion: pharmacological stability of vancomycin hydrochloride (5 mg/mL) and physical incompatibility with heparin sodium (100 IU/mL) were evidenced after three hours of association in the antimicrobial seal solutions studied.
Resumo Objetivo: verificar a estabilidade do cloridrato de vancomicina em soluções de selo antimicrobiano sem e com associação de heparina sódica segundo a temperatura e tempo de associação. Método: estudo experimental delineado para análise de potencial hidrogeniônico e concentração por cromatografia líquida de alta eficiência de soluções de cloridrato de vancomicina (n=06) e cloridrato de vancomicina e heparina sódica (n=06). Submeteram-se as soluções estudadas à ausência de luz, 22°C e 37°C. Análises em triplicadas (n=192) ocorreram no momento inicial (T0), três (T3), oito (T8) e 24 horas (T24) após preparo. Os dados foram submetidos à análise de variância (p≤0,05). Resultados: a concentração do antimicrobiano a 22°C apresentou redução (T0-T8) e posterior elevação (T24); o potencial hidrogeniônico diminuiu significativamente ao longo do tempo. Em 37°C a concentração aumentou em até T3 e reduziu em T24, com redução de potencial hidrogeniônico até 24 horas. A concentração das soluções de cloridrato de vancomicina e heparina sódica apresentaram variação com redução a 22°C acompanhada de aumento de potencial hidrogeniônico. Observou-se formação de precipitado por inspeção visual da associação cloridrato de vancomicina e heparina sódica (T3). Conclusão: evidenciou-se estabilidade farmacológica do cloridrato de vancomicina (5 mg/mL) e incompatibilidade física com heparina sódica (100 UI/mL) após três horas de associação nas soluções de selo antimicrobiano estudadas.
Resumen Objetivo: verificar la estabilidad del clorhidrato de vancomicina en soluciones de sellado antimicrobiano solo y combinado con heparina sódica según la temperatura y el tiempo de combinación. Método: estudio experimental diseñado para analizar el potencial de hidrógeno y la concentración por cromatografía líquida de alta resolución de soluciones de clorhidrato de vancomicina (n=06) y de clorhidrato de vancomicina y heparina sódica (n=06). Las soluciones estudiadas fueron sometidas a ausencia de luz, 22°C y 37°C. Se realizaron análisis por triplicado (n=192) en el momento inicial (T0), a las tres (T3), ocho (T8) y 24 horas (T24) después de la preparación. Los datos fueron sometidos a análisis de varianza (p≤0,05). Resultados: la concentración de antimicrobiano a 22°C mostró una reducción (T0-T8) y un posterior aumento (T24); el potencial de hidrógeno disminuyó significativamente con el tiempo. A 37°C, la concentración aumentó hasta T3 y disminuyó en T24, el potencial de hidrógeno disminuyó hasta las 24 horas. La concentración de las soluciones de clorhidrato de vancomicina y heparina sódica mostró variación con la reducción a 22°C acompañada de un aumento del potencial de hidrógeno. Mediante inspección visual se observó la formación de un precipitado al combinar clorhidrato de vancomicina y heparina sódica (T3). Conclusión: el clorhidrato de vancomicina (5 mg/ml) presentó evidencia de estabilidad farmacológica e incompatibilidad física con la heparina sódica (100 UI/ml) después de las tres horas de haberse realizado la combinación en las soluciones de sellado antimicrobiano estudiadas.</description><subject>Antibiotics</subject><subject>Anticoagulants</subject><subject>Antimicrobial agents</subject><subject>Catheters</subject><subject>Disease prevention</subject><subject>Intravenous therapy</subject><subject>Sodium</subject><issn>1518-8345</issn><issn>0104-1169</issn><issn>1518-8345</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpNkE9LxDAQxYMouK5-Ai8Fz62ZptmmR1n8Bwse1HNIkymbpW3WJF3ot7dhPXiZN7x5zDA_Qu6BFsAb-ggcRCFYxQsuNk3BNiVckFVy8-Re_uuvyU0IB0rLBiisiPuMqrW9jXPmuuykRu2GWdsx28_GO73vnbcGMxyOvZvRZMtEjdEOVnvXWtVnAVNx_RStG0NaonGMfjFtkhOObgqZVnGPEX24JVed6gPe_emafL88f23f8t3H6_v2aZdrgBpyVJq2gFo0lTJGV3WJrWDtRovaMK6poG0lKIeyLhtUnKMujUHRmUZVXcUEW5OH896jdz8ThigPbvLjclKWNYiac1bBkmLn1PJNCB47efR2UH6WQGUiKxM3mbjJRFYmsuwX3Idu7Q</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Barros, Daniele Porto</creator><creator>Onofre, Priscilla Sete de Carvalho</creator><creator>Fonseca, Fernando Luiz Affonso</creator><creator>Rosa, Paulo César Pires</creator><creator>Pedreira, Mavilde da Luz Gonçalves</creator><creator>Peterlini, Maria Angélica Sorgini</creator><general>Universidade de São Paulo-USP, Escola de Enfermagem de Ribeirão Preto - USP</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>KB0</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><orcidid>https://orcid.org/0000-0003-1769-4662</orcidid><orcidid>https://orcid.org/0000-0003-3665-8878</orcidid><orcidid>https://orcid.org/0000-0002-4388-3793</orcidid><orcidid>https://orcid.org/0000-0002-8830-0993</orcidid><orcidid>https://orcid.org/0000-0002-9246-2354</orcidid><orcidid>https://orcid.org/0000-0003-1223-1589</orcidid></search><sort><creationdate>2022</creationdate><title>Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters</title><author>Barros, Daniele Porto ; Onofre, Priscilla Sete de Carvalho ; Fonseca, Fernando Luiz Affonso ; Rosa, Paulo César Pires ; Pedreira, Mavilde da Luz Gonçalves ; Peterlini, Maria Angélica Sorgini</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1171-eac0b1ec894addc472eb83b6c87d35c080b480512729ea55ec2dde8fd9a4f4383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antibiotics</topic><topic>Anticoagulants</topic><topic>Antimicrobial agents</topic><topic>Catheters</topic><topic>Disease prevention</topic><topic>Intravenous therapy</topic><topic>Sodium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barros, Daniele Porto</creatorcontrib><creatorcontrib>Onofre, Priscilla Sete de Carvalho</creatorcontrib><creatorcontrib>Fonseca, Fernando Luiz Affonso</creatorcontrib><creatorcontrib>Rosa, Paulo César Pires</creatorcontrib><creatorcontrib>Pedreira, Mavilde da Luz Gonçalves</creatorcontrib><creatorcontrib>Peterlini, Maria Angélica Sorgini</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Revista latino-americana de enfermagem</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barros, Daniele Porto</au><au>Onofre, Priscilla Sete de Carvalho</au><au>Fonseca, Fernando Luiz Affonso</au><au>Rosa, Paulo César Pires</au><au>Pedreira, Mavilde da Luz Gonçalves</au><au>Peterlini, Maria Angélica Sorgini</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters</atitle><jtitle>Revista latino-americana de enfermagem</jtitle><date>2022</date><risdate>2022</risdate><volume>30</volume><issn>1518-8345</issn><issn>0104-1169</issn><eissn>1518-8345</eissn><abstract>Abstract Objective: to verify the stability of vancomycin hydrochloride in antimicrobial seal solutions with and without association of heparin sodium according to temperature and association time. Method: an experimental study designed for the analysis of hydrogenionic potential and concentration by means of high-efficiency liquid chromatography of vancomycin hydrochloride (n=06) and vancomycin hydrochloride and heparin sodium (n=06). The solutions studied were submitted to absence of light, as well as to 22°C and 37°C. Analyses in triplicate (n=192) were performed at the initial moment (T0) and three (T3), eight (T8) and 24 hours (T24) after preparation. The data were submitted to analysis of variance (p≤0.05). Results: concentration of the antimicrobial at 22°C presented a reduction (T0-T8) and a subsequent increase (T24); hydrogenionic potential decreased significantly over time. At 37°C, the concentration increased up to T3 and decreased at T24, with a reduction of hydrogenionic potential up to 24 hours. Concentration of the vancomycin hydrochloride and heparin sodium solutions varied with a reduction at 22°C, accompanied by increased hydrogenionic potential. Precipitate formation was observed by visual inspection of the vancomycin hydrochloride-heparin sodium association (T3). Conclusion: pharmacological stability of vancomycin hydrochloride (5 mg/mL) and physical incompatibility with heparin sodium (100 IU/mL) were evidenced after three hours of association in the antimicrobial seal solutions studied.
Resumo Objetivo: verificar a estabilidade do cloridrato de vancomicina em soluções de selo antimicrobiano sem e com associação de heparina sódica segundo a temperatura e tempo de associação. Método: estudo experimental delineado para análise de potencial hidrogeniônico e concentração por cromatografia líquida de alta eficiência de soluções de cloridrato de vancomicina (n=06) e cloridrato de vancomicina e heparina sódica (n=06). Submeteram-se as soluções estudadas à ausência de luz, 22°C e 37°C. Análises em triplicadas (n=192) ocorreram no momento inicial (T0), três (T3), oito (T8) e 24 horas (T24) após preparo. Os dados foram submetidos à análise de variância (p≤0,05). Resultados: a concentração do antimicrobiano a 22°C apresentou redução (T0-T8) e posterior elevação (T24); o potencial hidrogeniônico diminuiu significativamente ao longo do tempo. Em 37°C a concentração aumentou em até T3 e reduziu em T24, com redução de potencial hidrogeniônico até 24 horas. A concentração das soluções de cloridrato de vancomicina e heparina sódica apresentaram variação com redução a 22°C acompanhada de aumento de potencial hidrogeniônico. Observou-se formação de precipitado por inspeção visual da associação cloridrato de vancomicina e heparina sódica (T3). Conclusão: evidenciou-se estabilidade farmacológica do cloridrato de vancomicina (5 mg/mL) e incompatibilidade física com heparina sódica (100 UI/mL) após três horas de associação nas soluções de selo antimicrobiano estudadas.
Resumen Objetivo: verificar la estabilidad del clorhidrato de vancomicina en soluciones de sellado antimicrobiano solo y combinado con heparina sódica según la temperatura y el tiempo de combinación. Método: estudio experimental diseñado para analizar el potencial de hidrógeno y la concentración por cromatografía líquida de alta resolución de soluciones de clorhidrato de vancomicina (n=06) y de clorhidrato de vancomicina y heparina sódica (n=06). Las soluciones estudiadas fueron sometidas a ausencia de luz, 22°C y 37°C. Se realizaron análisis por triplicado (n=192) en el momento inicial (T0), a las tres (T3), ocho (T8) y 24 horas (T24) después de la preparación. Los datos fueron sometidos a análisis de varianza (p≤0,05). Resultados: la concentración de antimicrobiano a 22°C mostró una reducción (T0-T8) y un posterior aumento (T24); el potencial de hidrógeno disminuyó significativamente con el tiempo. A 37°C, la concentración aumentó hasta T3 y disminuyó en T24, el potencial de hidrógeno disminuyó hasta las 24 horas. La concentración de las soluciones de clorhidrato de vancomicina y heparina sódica mostró variación con la reducción a 22°C acompañada de un aumento del potencial de hidrógeno. Mediante inspección visual se observó la formación de un precipitado al combinar clorhidrato de vancomicina y heparina sódica (T3). Conclusión: el clorhidrato de vancomicina (5 mg/ml) presentó evidencia de estabilidad farmacológica e incompatibilidad física con la heparina sódica (100 UI/ml) después de las tres horas de haberse realizado la combinación en las soluciones de sellado antimicrobiano estudiadas.</abstract><cop>Sao Paulo</cop><pub>Universidade de São Paulo-USP, Escola de Enfermagem de Ribeirão Preto - USP</pub><doi>10.1590/1518.8345.5869.3621</doi><orcidid>https://orcid.org/0000-0003-1769-4662</orcidid><orcidid>https://orcid.org/0000-0003-3665-8878</orcidid><orcidid>https://orcid.org/0000-0002-4388-3793</orcidid><orcidid>https://orcid.org/0000-0002-8830-0993</orcidid><orcidid>https://orcid.org/0000-0002-9246-2354</orcidid><orcidid>https://orcid.org/0000-0003-1223-1589</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1518-8345 |
| ispartof | Revista latino-americana de enfermagem, 2022, Vol.30 |
| issn | 1518-8345 0104-1169 1518-8345 |
| language | eng |
| recordid | cdi_proquest_journals_2718755341 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | Antibiotics Anticoagulants Antimicrobial agents Catheters Disease prevention Intravenous therapy Sodium |
| title | Stability of vancomycin hydrochloride employed in antimicrobial seal solutions of central intravenous catheters |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T14%3A51%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Stability%20of%20vancomycin%20hydrochloride%20employed%20in%20antimicrobial%20seal%20solutions%20of%20central%20intravenous%20catheters&rft.jtitle=Revista%20latino-americana%20de%20enfermagem&rft.au=Barros,%20Daniele%20Porto&rft.date=2022&rft.volume=30&rft.issn=1518-8345&rft.eissn=1518-8345&rft_id=info:doi/10.1590/1518.8345.5869.3621&rft_dat=%3Cproquest_cross%3E2718755341%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2718755341&rft_id=info:pmid/&rfr_iscdi=true |