Dental follicle mesenchymal stem cells regulate responses in sepsis
Objective: Sepsis-induced immune alterations are associated with secondary infections and increased risk of death. The use of mesenchymal stem cells (MSCs) has been described as a novel therapeutic strategy. We evaluated the immunomodulatory effects of human dental follicle (DF-MSCs) on lymphocytes...
Gespeichert in:
| Veröffentlicht in: | Marmara Medical Journal 2020-01, Vol.33 (1), p.7-16 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 16 |
|---|---|
| container_issue | 1 |
| container_start_page | 7 |
| container_title | Marmara Medical Journal |
| container_volume | 33 |
| creator | GUL, Fethi GENC, Deniz ARSLANTAS, Mustafa Kemal ZIBANDEH, Noushin TOPCU, Leyla AKKOC, Tunç CINEL, İsmail |
| description | Objective: Sepsis-induced immune alterations are associated with secondary infections and increased risk of death. The use of mesenchymal stem cells (MSCs) has been described as a novel therapeutic strategy. We evaluated the immunomodulatory effects of human dental follicle (DF-MSCs) on lymphocytes of sepsis and septic shock patients.
Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood samples of sepsis, septic shock and healthy subjects. PBMCs were co-cultured in the presence and absence of DF-MSCs with or without interferon-gamma (IFN-γ) for 72 hours. CD4+CD25+FoxP3+regulatory T (Treg) cell frequency, lymphocyte proliferation, cytokine levels and apoptosis were evaluated via flow cytometry.
Results: DF-MSCs significantly suppressed proliferation of lymphocytes in sepsis group compared to septic shock group (p |
| doi_str_mv | 10.5472/marumj.681996 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2718715724</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2718715724</sourcerecordid><originalsourceid>FETCH-LOGICAL-c260t-cf15bc7c798c42d38d8712626794df39b805961b98e2487f6662dbfdbce1e07d3</originalsourceid><addsrcrecordid>eNotkMtLxDAYxIMouK4evRc8d03SNI-j1CcseNFzaNMv2pI-zNce9r83Uk8zDMMM_Ai5ZfRQCsXvhzquQ3-Qmhkjz8iOFdTkRkhznjxlJmdGsEtyhdhTWmpq5I5UjzAudcj8FELnAmQDIIzu-zSkEBcYMgchYBbhaw31AsngPI0ImHVjhjBjh9fkwtcB4eZf9-Tz-emjes2P7y9v1cMxd1zSJXeelY1TThntBG8L3WrFuORSGdH6wjSalkayxmjgQisvpeRt49vGAQOq2mJP7rbdOU4_K-Bi-2mNY7q0XLE0ViouUivfWi5OiBG8nWOX0Jwso_aPk9042Y1T8Qvz0lzI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2718715724</pqid></control><display><type>article</type><title>Dental follicle mesenchymal stem cells regulate responses in sepsis</title><source>EZB-FREE-00999 freely available EZB journals</source><creator>GUL, Fethi ; GENC, Deniz ; ARSLANTAS, Mustafa Kemal ; ZIBANDEH, Noushin ; TOPCU, Leyla ; AKKOC, Tunç ; CINEL, İsmail</creator><creatorcontrib>GUL, Fethi ; GENC, Deniz ; ARSLANTAS, Mustafa Kemal ; ZIBANDEH, Noushin ; TOPCU, Leyla ; AKKOC, Tunç ; CINEL, İsmail</creatorcontrib><description>Objective: Sepsis-induced immune alterations are associated with secondary infections and increased risk of death. The use of mesenchymal stem cells (MSCs) has been described as a novel therapeutic strategy. We evaluated the immunomodulatory effects of human dental follicle (DF-MSCs) on lymphocytes of sepsis and septic shock patients.
Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood samples of sepsis, septic shock and healthy subjects. PBMCs were co-cultured in the presence and absence of DF-MSCs with or without interferon-gamma (IFN-γ) for 72 hours. CD4+CD25+FoxP3+regulatory T (Treg) cell frequency, lymphocyte proliferation, cytokine levels and apoptosis were evaluated via flow cytometry.
Results: DF-MSCs significantly suppressed proliferation of lymphocytes in sepsis group compared to septic shock group (p<0.005). DF-MSCs remarkably increased Treg ratio in sepsis compared to control group (p<0.05). Reduction of lymphocyte apoptosis in cocultures of DF-MSCs and PBMC was significant in both sepsis and septic shock groups. IFN-γ stimulation of DF-MSCs ameliorated shift in the T-cell subsets from Th2 to Th1 phenotype in septic shock.
Conclusion: Our findings revealed that DF-MSCs have immunoregulatory effects both in sepsis and septic shock, by reducing interleukin-4 (IL-4) and increasing IFN-γ levels. This immunoreactivity regulation may open new therapeutic approaches for septic shock patients.</description><identifier>ISSN: 1019-1941</identifier><identifier>EISSN: 1309-9469</identifier><identifier>DOI: 10.5472/marumj.681996</identifier><language>eng</language><publisher>Istanbul: Marmara University</publisher><subject>Apoptosis ; Lymphocytes ; Sepsis ; Stem cells</subject><ispartof>Marmara Medical Journal, 2020-01, Vol.33 (1), p.7-16</ispartof><rights>2020. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the associated terms available at https://dergipark.org.tr/en/pub/marumj/about-journal</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c260t-cf15bc7c798c42d38d8712626794df39b805961b98e2487f6662dbfdbce1e07d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>GUL, Fethi</creatorcontrib><creatorcontrib>GENC, Deniz</creatorcontrib><creatorcontrib>ARSLANTAS, Mustafa Kemal</creatorcontrib><creatorcontrib>ZIBANDEH, Noushin</creatorcontrib><creatorcontrib>TOPCU, Leyla</creatorcontrib><creatorcontrib>AKKOC, Tunç</creatorcontrib><creatorcontrib>CINEL, İsmail</creatorcontrib><title>Dental follicle mesenchymal stem cells regulate responses in sepsis</title><title>Marmara Medical Journal</title><description>Objective: Sepsis-induced immune alterations are associated with secondary infections and increased risk of death. The use of mesenchymal stem cells (MSCs) has been described as a novel therapeutic strategy. We evaluated the immunomodulatory effects of human dental follicle (DF-MSCs) on lymphocytes of sepsis and septic shock patients.
Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood samples of sepsis, septic shock and healthy subjects. PBMCs were co-cultured in the presence and absence of DF-MSCs with or without interferon-gamma (IFN-γ) for 72 hours. CD4+CD25+FoxP3+regulatory T (Treg) cell frequency, lymphocyte proliferation, cytokine levels and apoptosis were evaluated via flow cytometry.
Results: DF-MSCs significantly suppressed proliferation of lymphocytes in sepsis group compared to septic shock group (p<0.005). DF-MSCs remarkably increased Treg ratio in sepsis compared to control group (p<0.05). Reduction of lymphocyte apoptosis in cocultures of DF-MSCs and PBMC was significant in both sepsis and septic shock groups. IFN-γ stimulation of DF-MSCs ameliorated shift in the T-cell subsets from Th2 to Th1 phenotype in septic shock.
Conclusion: Our findings revealed that DF-MSCs have immunoregulatory effects both in sepsis and septic shock, by reducing interleukin-4 (IL-4) and increasing IFN-γ levels. This immunoreactivity regulation may open new therapeutic approaches for septic shock patients.</description><subject>Apoptosis</subject><subject>Lymphocytes</subject><subject>Sepsis</subject><subject>Stem cells</subject><issn>1019-1941</issn><issn>1309-9469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNotkMtLxDAYxIMouK4evRc8d03SNI-j1CcseNFzaNMv2pI-zNce9r83Uk8zDMMM_Ai5ZfRQCsXvhzquQ3-Qmhkjz8iOFdTkRkhznjxlJmdGsEtyhdhTWmpq5I5UjzAudcj8FELnAmQDIIzu-zSkEBcYMgchYBbhaw31AsngPI0ImHVjhjBjh9fkwtcB4eZf9-Tz-emjes2P7y9v1cMxd1zSJXeelY1TThntBG8L3WrFuORSGdH6wjSalkayxmjgQisvpeRt49vGAQOq2mJP7rbdOU4_K-Bi-2mNY7q0XLE0ViouUivfWi5OiBG8nWOX0Jwso_aPk9042Y1T8Qvz0lzI</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>GUL, Fethi</creator><creator>GENC, Deniz</creator><creator>ARSLANTAS, Mustafa Kemal</creator><creator>ZIBANDEH, Noushin</creator><creator>TOPCU, Leyla</creator><creator>AKKOC, Tunç</creator><creator>CINEL, İsmail</creator><general>Marmara University</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20200101</creationdate><title>Dental follicle mesenchymal stem cells regulate responses in sepsis</title><author>GUL, Fethi ; GENC, Deniz ; ARSLANTAS, Mustafa Kemal ; ZIBANDEH, Noushin ; TOPCU, Leyla ; AKKOC, Tunç ; CINEL, İsmail</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c260t-cf15bc7c798c42d38d8712626794df39b805961b98e2487f6662dbfdbce1e07d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Apoptosis</topic><topic>Lymphocytes</topic><topic>Sepsis</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GUL, Fethi</creatorcontrib><creatorcontrib>GENC, Deniz</creatorcontrib><creatorcontrib>ARSLANTAS, Mustafa Kemal</creatorcontrib><creatorcontrib>ZIBANDEH, Noushin</creatorcontrib><creatorcontrib>TOPCU, Leyla</creatorcontrib><creatorcontrib>AKKOC, Tunç</creatorcontrib><creatorcontrib>CINEL, İsmail</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Marmara Medical Journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GUL, Fethi</au><au>GENC, Deniz</au><au>ARSLANTAS, Mustafa Kemal</au><au>ZIBANDEH, Noushin</au><au>TOPCU, Leyla</au><au>AKKOC, Tunç</au><au>CINEL, İsmail</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dental follicle mesenchymal stem cells regulate responses in sepsis</atitle><jtitle>Marmara Medical Journal</jtitle><date>2020-01-01</date><risdate>2020</risdate><volume>33</volume><issue>1</issue><spage>7</spage><epage>16</epage><pages>7-16</pages><issn>1019-1941</issn><eissn>1309-9469</eissn><abstract>Objective: Sepsis-induced immune alterations are associated with secondary infections and increased risk of death. The use of mesenchymal stem cells (MSCs) has been described as a novel therapeutic strategy. We evaluated the immunomodulatory effects of human dental follicle (DF-MSCs) on lymphocytes of sepsis and septic shock patients.
Materials and Methods: Peripheral blood mononuclear cells (PBMCs) were isolated from venous blood samples of sepsis, septic shock and healthy subjects. PBMCs were co-cultured in the presence and absence of DF-MSCs with or without interferon-gamma (IFN-γ) for 72 hours. CD4+CD25+FoxP3+regulatory T (Treg) cell frequency, lymphocyte proliferation, cytokine levels and apoptosis were evaluated via flow cytometry.
Results: DF-MSCs significantly suppressed proliferation of lymphocytes in sepsis group compared to septic shock group (p<0.005). DF-MSCs remarkably increased Treg ratio in sepsis compared to control group (p<0.05). Reduction of lymphocyte apoptosis in cocultures of DF-MSCs and PBMC was significant in both sepsis and septic shock groups. IFN-γ stimulation of DF-MSCs ameliorated shift in the T-cell subsets from Th2 to Th1 phenotype in septic shock.
Conclusion: Our findings revealed that DF-MSCs have immunoregulatory effects both in sepsis and septic shock, by reducing interleukin-4 (IL-4) and increasing IFN-γ levels. This immunoreactivity regulation may open new therapeutic approaches for septic shock patients.</abstract><cop>Istanbul</cop><pub>Marmara University</pub><doi>10.5472/marumj.681996</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1019-1941 |
| ispartof | Marmara Medical Journal, 2020-01, Vol.33 (1), p.7-16 |
| issn | 1019-1941 1309-9469 |
| language | eng |
| recordid | cdi_proquest_journals_2718715724 |
| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Apoptosis Lymphocytes Sepsis Stem cells |
| title | Dental follicle mesenchymal stem cells regulate responses in sepsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T10%3A01%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dental%20follicle%20mesenchymal%20stem%20cells%20regulate%20responses%20in%20sepsis&rft.jtitle=Marmara%20Medical%20Journal&rft.au=GUL,%20Fethi&rft.date=2020-01-01&rft.volume=33&rft.issue=1&rft.spage=7&rft.epage=16&rft.pages=7-16&rft.issn=1019-1941&rft.eissn=1309-9469&rft_id=info:doi/10.5472/marumj.681996&rft_dat=%3Cproquest_cross%3E2718715724%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2718715724&rft_id=info:pmid/&rfr_iscdi=true |