Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-β1 and evaluation of in vitro wound healing effect
Wound healing involves many complex mechanisms, and many growth factors are effective in this process. Growth factors are biologically active polypeptides. They perform activities such as cell growth, differentiation, proliferation and migration with molecular cascades by binding to specific recepto...
Gespeichert in:
| Veröffentlicht in: | Journal of Research in Pharmacy 2020-01, Vol.24 (2), p.277 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | 277 |
| container_title | Journal of Research in Pharmacy |
| container_volume | 24 |
| creator | SOYSAL, Aysun ÇELİK Sevinç ŞAHBAZ UĞURLU, Timuçin Ali Demir SEZER |
| description | Wound healing involves many complex mechanisms, and many growth factors are effective in this process. Growth factors are biologically active polypeptides. They perform activities such as cell growth, differentiation, proliferation and migration with molecular cascades by binding to specific receptors. Transforming growth factor stimulates (TGF-β) different cell types in the wound healing process. Poly(lactic-co-glycolic acid) (PLGA) degradation produces lactate that expedites angiogenesis, activates pro-collagen factors. Therewith, we hypothesized to combine the therapeutic effect of the TGF-β1with the positive effect of the drug delivery system including PLGA nanoparticles (TGF-β- PLGA NP). The burst effect decreases as the polymer concentration increases in PLGA nanoparticles. The inhibitory effect of TGF-β1 on keratinocytes was reduced by the improved nanoparticle formulations. It showed a proliferative effect of up to 92.5 per cent on fibroblast cells involved in wound healing. Although TGF-β1 has an inhibitory effect on keratinocytes, it induces migration both NIH-3T3 and HaCaT cell lines in the scratch assay. |
| doi_str_mv | 10.35333/jrp.2020.144 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_journals_2718688819</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2718688819</sourcerecordid><originalsourceid>FETCH-LOGICAL-p98t-902b891cc1b29ccfc43635d67afd8f28f72ea0303cee68608e488796f8fdb85a3</originalsourceid><addsrcrecordid>eNpNj81KAzEUhYMoWGqX7gNudJExPzOZzFKKrUJBF92X9E7SpoRknJ9KfQwfxQfxmYxWxNW99xzOd7gIXTKaiUIIcbtrm4xTTjOW5ydoxKWgRIo8P_23n6NJ1-0opVxVLOfFCL0_t6bRre5dDFiHGsM2XdCb1r0dxWhxE_3h2ifVAYFINv4A0TvAGlx9g4MOMSGS6U2HIYZeu-DCBi_nM_L5wX6wZq_98Ad0Ae9d30b8Godkbo323wFjrYH-Ap1Z7Tsz-Z1jtJzdL6cPZPE0f5zeLUhTqZ5UlK_TEwBszSsAC7mQoqhlqW2tLFe25EZTQQUYI5WkyuRKlZW0ytZrVWgxRldHbNPGl8F0_WoXhzakxhUvmZJKKVaJL1BXaw8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2718688819</pqid></control><display><type>article</type><title>Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-β1 and evaluation of in vitro wound healing effect</title><source>Alma/SFX Local Collection</source><creator>SOYSAL, Aysun ÇELİK ; Sevinç ŞAHBAZ ; UĞURLU, Timuçin ; Ali Demir SEZER</creator><creatorcontrib>SOYSAL, Aysun ÇELİK ; Sevinç ŞAHBAZ ; UĞURLU, Timuçin ; Ali Demir SEZER</creatorcontrib><description>Wound healing involves many complex mechanisms, and many growth factors are effective in this process. Growth factors are biologically active polypeptides. They perform activities such as cell growth, differentiation, proliferation and migration with molecular cascades by binding to specific receptors. Transforming growth factor stimulates (TGF-β) different cell types in the wound healing process. Poly(lactic-co-glycolic acid) (PLGA) degradation produces lactate that expedites angiogenesis, activates pro-collagen factors. Therewith, we hypothesized to combine the therapeutic effect of the TGF-β1with the positive effect of the drug delivery system including PLGA nanoparticles (TGF-β- PLGA NP). The burst effect decreases as the polymer concentration increases in PLGA nanoparticles. The inhibitory effect of TGF-β1 on keratinocytes was reduced by the improved nanoparticle formulations. It showed a proliferative effect of up to 92.5 per cent on fibroblast cells involved in wound healing. Although TGF-β1 has an inhibitory effect on keratinocytes, it induces migration both NIH-3T3 and HaCaT cell lines in the scratch assay.</description><identifier>ISSN: 2630-6344</identifier><identifier>EISSN: 2630-6344</identifier><identifier>DOI: 10.35333/jrp.2020.144</identifier><language>eng</language><publisher>Istanbul: Marmara University</publisher><subject>Growth factors ; Nanoparticles ; Wound healing</subject><ispartof>Journal of Research in Pharmacy, 2020-01, Vol.24 (2), p.277</ispartof><rights>2020. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the associated terms available at https://jrespharm.com/static.php?id=7</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>SOYSAL, Aysun ÇELİK</creatorcontrib><creatorcontrib>Sevinç ŞAHBAZ</creatorcontrib><creatorcontrib>UĞURLU, Timuçin</creatorcontrib><creatorcontrib>Ali Demir SEZER</creatorcontrib><title>Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-β1 and evaluation of in vitro wound healing effect</title><title>Journal of Research in Pharmacy</title><description>Wound healing involves many complex mechanisms, and many growth factors are effective in this process. Growth factors are biologically active polypeptides. They perform activities such as cell growth, differentiation, proliferation and migration with molecular cascades by binding to specific receptors. Transforming growth factor stimulates (TGF-β) different cell types in the wound healing process. Poly(lactic-co-glycolic acid) (PLGA) degradation produces lactate that expedites angiogenesis, activates pro-collagen factors. Therewith, we hypothesized to combine the therapeutic effect of the TGF-β1with the positive effect of the drug delivery system including PLGA nanoparticles (TGF-β- PLGA NP). The burst effect decreases as the polymer concentration increases in PLGA nanoparticles. The inhibitory effect of TGF-β1 on keratinocytes was reduced by the improved nanoparticle formulations. It showed a proliferative effect of up to 92.5 per cent on fibroblast cells involved in wound healing. Although TGF-β1 has an inhibitory effect on keratinocytes, it induces migration both NIH-3T3 and HaCaT cell lines in the scratch assay.</description><subject>Growth factors</subject><subject>Nanoparticles</subject><subject>Wound healing</subject><issn>2630-6344</issn><issn>2630-6344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpNj81KAzEUhYMoWGqX7gNudJExPzOZzFKKrUJBF92X9E7SpoRknJ9KfQwfxQfxmYxWxNW99xzOd7gIXTKaiUIIcbtrm4xTTjOW5ydoxKWgRIo8P_23n6NJ1-0opVxVLOfFCL0_t6bRre5dDFiHGsM2XdCb1r0dxWhxE_3h2ifVAYFINv4A0TvAGlx9g4MOMSGS6U2HIYZeu-DCBi_nM_L5wX6wZq_98Ad0Ae9d30b8Godkbo323wFjrYH-Ap1Z7Tsz-Z1jtJzdL6cPZPE0f5zeLUhTqZ5UlK_TEwBszSsAC7mQoqhlqW2tLFe25EZTQQUYI5WkyuRKlZW0ytZrVWgxRldHbNPGl8F0_WoXhzakxhUvmZJKKVaJL1BXaw8</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>SOYSAL, Aysun ÇELİK</creator><creator>Sevinç ŞAHBAZ</creator><creator>UĞURLU, Timuçin</creator><creator>Ali Demir SEZER</creator><general>Marmara University</general><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20200101</creationdate><title>Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-β1 and evaluation of in vitro wound healing effect</title><author>SOYSAL, Aysun ÇELİK ; Sevinç ŞAHBAZ ; UĞURLU, Timuçin ; Ali Demir SEZER</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p98t-902b891cc1b29ccfc43635d67afd8f28f72ea0303cee68608e488796f8fdb85a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Growth factors</topic><topic>Nanoparticles</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SOYSAL, Aysun ÇELİK</creatorcontrib><creatorcontrib>Sevinç ŞAHBAZ</creatorcontrib><creatorcontrib>UĞURLU, Timuçin</creatorcontrib><creatorcontrib>Ali Demir SEZER</creatorcontrib><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of Research in Pharmacy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SOYSAL, Aysun ÇELİK</au><au>Sevinç ŞAHBAZ</au><au>UĞURLU, Timuçin</au><au>Ali Demir SEZER</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-β1 and evaluation of in vitro wound healing effect</atitle><jtitle>Journal of Research in Pharmacy</jtitle><date>2020-01-01</date><risdate>2020</risdate><volume>24</volume><issue>2</issue><spage>277</spage><pages>277-</pages><issn>2630-6344</issn><eissn>2630-6344</eissn><abstract>Wound healing involves many complex mechanisms, and many growth factors are effective in this process. Growth factors are biologically active polypeptides. They perform activities such as cell growth, differentiation, proliferation and migration with molecular cascades by binding to specific receptors. Transforming growth factor stimulates (TGF-β) different cell types in the wound healing process. Poly(lactic-co-glycolic acid) (PLGA) degradation produces lactate that expedites angiogenesis, activates pro-collagen factors. Therewith, we hypothesized to combine the therapeutic effect of the TGF-β1with the positive effect of the drug delivery system including PLGA nanoparticles (TGF-β- PLGA NP). The burst effect decreases as the polymer concentration increases in PLGA nanoparticles. The inhibitory effect of TGF-β1 on keratinocytes was reduced by the improved nanoparticle formulations. It showed a proliferative effect of up to 92.5 per cent on fibroblast cells involved in wound healing. Although TGF-β1 has an inhibitory effect on keratinocytes, it induces migration both NIH-3T3 and HaCaT cell lines in the scratch assay.</abstract><cop>Istanbul</cop><pub>Marmara University</pub><doi>10.35333/jrp.2020.144</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2630-6344 |
| ispartof | Journal of Research in Pharmacy, 2020-01, Vol.24 (2), p.277 |
| issn | 2630-6344 2630-6344 |
| language | eng |
| recordid | cdi_proquest_journals_2718688819 |
| source | Alma/SFX Local Collection |
| subjects | Growth factors Nanoparticles Wound healing |
| title | Preparation and characterization of poly(lactic-co-glycolic acid) nanoparticles containing TGF-β1 and evaluation of in vitro wound healing effect |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T06%3A21%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20characterization%20of%20poly(lactic-co-glycolic%20acid)%20nanoparticles%20containing%20TGF-%CE%B21%20and%20evaluation%20of%20in%20vitro%20wound%20healing%20effect&rft.jtitle=Journal%20of%20Research%20in%20Pharmacy&rft.au=SOYSAL,%20Aysun%20%C3%87EL%C4%B0K&rft.date=2020-01-01&rft.volume=24&rft.issue=2&rft.spage=277&rft.pages=277-&rft.issn=2630-6344&rft.eissn=2630-6344&rft_id=info:doi/10.35333/jrp.2020.144&rft_dat=%3Cproquest%3E2718688819%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2718688819&rft_id=info:pmid/&rfr_iscdi=true |