Phage Antibodies as Bioreceptors for Ampicillin Detection
Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibo...
Gespeichert in:
| Veröffentlicht in: | Applied biochemistry and microbiology 2022-10, Vol.58 (5), p.646-651 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 651 |
|---|---|
| container_issue | 5 |
| container_start_page | 646 |
| container_title | Applied biochemistry and microbiology |
| container_volume | 58 |
| creator | Guliy, O. I. Alsowaidi, A. K. M. Fomin, A. S. Gabalov, K. P. Staroverov, S. A. Karavaeva, O. A. |
| description | Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibodies are specific for ampicillin and do not interact with other antibiotics as tetracycline and kanamycin, as well as with substances similar in chemical formula, that is, L-phenylalanine, L-tryptophan, and L-cysteine. It has been shown that the method of solid-phase immunoassay with visual consideration of the results makes detection of ampicillin with the minimum concentration of 1 μg/mL possible. Phage antibodies are promising for use as a sensitive (recognizing) element of sensory systems at the ampicillin detection. |
| doi_str_mv | 10.1134/S0003683822050088 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2716551262</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2716551262</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-43a47fa91ab85d6f920cfb5e5aa6fc44852dd5a32d88fba065cbf6cde22db8b63</originalsourceid><addsrcrecordid>eNp1kE1LxDAYhIMoWFd_gLeC52q-Nz3W1VVhQUE9l3yuWbpNTbIH_70tFTyIp5dh5pkXBoBLBK8RIvTmFUJIuCACY8ggFOIIFIhDURGI6TEoJrua_FNwltJulDUXdQHqlw-5tWXTZ6-C8TaVMpW3PkSr7ZBDTKULsWz2g9e-63xf3tlsdfahPwcnTnbJXvzcBXhf37-tHqvN88PTqtlUmiCeK0okXTpZI6kEM9zVGGqnmGVScqcpFQwbwyTBRginJORMK8e1sRgbJRQnC3A19w4xfB5syu0uHGI_vmzxEnHGEOZ4TKE5pWNIKVrXDtHvZfxqEWynhdo_C40Mnpk0Zvutjb_N_0PflXRniw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2716551262</pqid></control><display><type>article</type><title>Phage Antibodies as Bioreceptors for Ampicillin Detection</title><source>SpringerLink Journals - AutoHoldings</source><creator>Guliy, O. I. ; Alsowaidi, A. K. M. ; Fomin, A. S. ; Gabalov, K. P. ; Staroverov, S. A. ; Karavaeva, O. A.</creator><creatorcontrib>Guliy, O. I. ; Alsowaidi, A. K. M. ; Fomin, A. S. ; Gabalov, K. P. ; Staroverov, S. A. ; Karavaeva, O. A.</creatorcontrib><description>Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibodies are specific for ampicillin and do not interact with other antibiotics as tetracycline and kanamycin, as well as with substances similar in chemical formula, that is, L-phenylalanine, L-tryptophan, and L-cysteine. It has been shown that the method of solid-phase immunoassay with visual consideration of the results makes detection of ampicillin with the minimum concentration of 1 μg/mL possible. Phage antibodies are promising for use as a sensitive (recognizing) element of sensory systems at the ampicillin detection.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683822050088</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Ampicillin ; Antibiotics ; Antibodies ; Biochemistry ; Biomedical and Life Sciences ; Immunoassay ; Kanamycin ; Life Sciences ; Medical Microbiology ; Microbiology ; Penicillin ; Phages ; Phenylalanine ; Sensory systems ; Solid phases ; Tryptophan</subject><ispartof>Applied biochemistry and microbiology, 2022-10, Vol.58 (5), p.646-651</ispartof><rights>Pleiades Publishing, Inc. 2022. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2022, Vol. 58, No. 5, pp. 646–651. © Pleiades Publishing, Inc., 2022. Russian Text © The Author(s), 2022, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2022, Vol. 58, No. 5, pp. 513–519.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-43a47fa91ab85d6f920cfb5e5aa6fc44852dd5a32d88fba065cbf6cde22db8b63</citedby><cites>FETCH-LOGICAL-c316t-43a47fa91ab85d6f920cfb5e5aa6fc44852dd5a32d88fba065cbf6cde22db8b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683822050088$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683822050088$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Guliy, O. I.</creatorcontrib><creatorcontrib>Alsowaidi, A. K. M.</creatorcontrib><creatorcontrib>Fomin, A. S.</creatorcontrib><creatorcontrib>Gabalov, K. P.</creatorcontrib><creatorcontrib>Staroverov, S. A.</creatorcontrib><creatorcontrib>Karavaeva, O. A.</creatorcontrib><title>Phage Antibodies as Bioreceptors for Ampicillin Detection</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibodies are specific for ampicillin and do not interact with other antibiotics as tetracycline and kanamycin, as well as with substances similar in chemical formula, that is, L-phenylalanine, L-tryptophan, and L-cysteine. It has been shown that the method of solid-phase immunoassay with visual consideration of the results makes detection of ampicillin with the minimum concentration of 1 μg/mL possible. Phage antibodies are promising for use as a sensitive (recognizing) element of sensory systems at the ampicillin detection.</description><subject>Ampicillin</subject><subject>Antibiotics</subject><subject>Antibodies</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Immunoassay</subject><subject>Kanamycin</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Penicillin</subject><subject>Phages</subject><subject>Phenylalanine</subject><subject>Sensory systems</subject><subject>Solid phases</subject><subject>Tryptophan</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LxDAYhIMoWFd_gLeC52q-Nz3W1VVhQUE9l3yuWbpNTbIH_70tFTyIp5dh5pkXBoBLBK8RIvTmFUJIuCACY8ggFOIIFIhDURGI6TEoJrua_FNwltJulDUXdQHqlw-5tWXTZ6-C8TaVMpW3PkSr7ZBDTKULsWz2g9e-63xf3tlsdfahPwcnTnbJXvzcBXhf37-tHqvN88PTqtlUmiCeK0okXTpZI6kEM9zVGGqnmGVScqcpFQwbwyTBRginJORMK8e1sRgbJRQnC3A19w4xfB5syu0uHGI_vmzxEnHGEOZ4TKE5pWNIKVrXDtHvZfxqEWynhdo_C40Mnpk0Zvutjb_N_0PflXRniw</recordid><startdate>20221001</startdate><enddate>20221001</enddate><creator>Guliy, O. I.</creator><creator>Alsowaidi, A. K. M.</creator><creator>Fomin, A. S.</creator><creator>Gabalov, K. P.</creator><creator>Staroverov, S. A.</creator><creator>Karavaeva, O. A.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20221001</creationdate><title>Phage Antibodies as Bioreceptors for Ampicillin Detection</title><author>Guliy, O. I. ; Alsowaidi, A. K. M. ; Fomin, A. S. ; Gabalov, K. P. ; Staroverov, S. A. ; Karavaeva, O. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-43a47fa91ab85d6f920cfb5e5aa6fc44852dd5a32d88fba065cbf6cde22db8b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Ampicillin</topic><topic>Antibiotics</topic><topic>Antibodies</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Immunoassay</topic><topic>Kanamycin</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Penicillin</topic><topic>Phages</topic><topic>Phenylalanine</topic><topic>Sensory systems</topic><topic>Solid phases</topic><topic>Tryptophan</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guliy, O. I.</creatorcontrib><creatorcontrib>Alsowaidi, A. K. M.</creatorcontrib><creatorcontrib>Fomin, A. S.</creatorcontrib><creatorcontrib>Gabalov, K. P.</creatorcontrib><creatorcontrib>Staroverov, S. A.</creatorcontrib><creatorcontrib>Karavaeva, O. A.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guliy, O. I.</au><au>Alsowaidi, A. K. M.</au><au>Fomin, A. S.</au><au>Gabalov, K. P.</au><au>Staroverov, S. A.</au><au>Karavaeva, O. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phage Antibodies as Bioreceptors for Ampicillin Detection</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2022-10-01</date><risdate>2022</risdate><volume>58</volume><issue>5</issue><spage>646</spage><epage>651</epage><pages>646-651</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay with biospecific interaction of selected recombinant antibodies in phage format (phage antibodies). It has been established that the phage antibodies are specific for ampicillin and do not interact with other antibiotics as tetracycline and kanamycin, as well as with substances similar in chemical formula, that is, L-phenylalanine, L-tryptophan, and L-cysteine. It has been shown that the method of solid-phase immunoassay with visual consideration of the results makes detection of ampicillin with the minimum concentration of 1 μg/mL possible. Phage antibodies are promising for use as a sensitive (recognizing) element of sensory systems at the ampicillin detection.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683822050088</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-6838 |
| ispartof | Applied biochemistry and microbiology, 2022-10, Vol.58 (5), p.646-651 |
| issn | 0003-6838 1608-3024 |
| language | eng |
| recordid | cdi_proquest_journals_2716551262 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Ampicillin Antibiotics Antibodies Biochemistry Biomedical and Life Sciences Immunoassay Kanamycin Life Sciences Medical Microbiology Microbiology Penicillin Phages Phenylalanine Sensory systems Solid phases Tryptophan |
| title | Phage Antibodies as Bioreceptors for Ampicillin Detection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T12%3A50%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Phage%20Antibodies%20as%20Bioreceptors%20for%20Ampicillin%20Detection&rft.jtitle=Applied%20biochemistry%20and%20microbiology&rft.au=Guliy,%20O.%20I.&rft.date=2022-10-01&rft.volume=58&rft.issue=5&rft.spage=646&rft.epage=651&rft.pages=646-651&rft.issn=0003-6838&rft.eissn=1608-3024&rft_id=info:doi/10.1134/S0003683822050088&rft_dat=%3Cproquest_cross%3E2716551262%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2716551262&rft_id=info:pmid/&rfr_iscdi=true |