Fixing carbon dioxide in situ during ethanol production by formate dehydrogenase
Concerns regarding the increasing carbon dioxide (CO 2 ) concentration has aroused global interest in renewable fuels and conversion of CO 2 into chemicals. This study provides a sustainable strategy for fixing CO 2 in situ during the production of ethanol in a single cell expressing formate dehydro...
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| Veröffentlicht in: | Green chemistry : an international journal and green chemistry resource : GC 2022-09, Vol.24 (18), p.6989-6999 |
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| container_title | Green chemistry : an international journal and green chemistry resource : GC |
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| creator | Du, Cong Li, Yimin he, Ying Su, Liming Wang, Huanan Yuan, Wenjie Bai, Fengwu |
| description | Concerns regarding the increasing carbon dioxide (CO
2
) concentration has aroused global interest in renewable fuels and conversion of CO
2
into chemicals. This study provides a sustainable strategy for fixing CO
2
in situ
during the production of ethanol in a single cell expressing formate dehydrogenase (FDH) without the addition of a cofactor. Glucose was converted to ethanol, with the main by-product CO
2
fixed to formate, increasing the yield of ethanol and glucose fermentation efficiency in the recombinant
Saccharomyces cerevisiae-fdh
. Higher concentrations of glucose and amount of ScFDH1 yielded more formate, confirming the feasibility of CO
2
fixation. Significantly, the glucose consumption rate and ethanol yield increased by 30% and 13% after the overexpression of ScFDH1, respectively. Finally, the increase in CO
2
concentration with the addition of zeolite imidazole framework-8 (ZIF-8) further demonstrated the CO
2
fixation
in situ
, increasing the production of formate 2.1 times. Due to ZIF-8, up to 60.67 mg L
−1
formate was produced. Our findings establish a green, economical, and sustainable alternative for CO
2
conversion
in situ
, contributing to the ethanol yield by ScFDH1 without extra energy input and cofactor supply. This synergistic strategy has significance for both the energy and environmental crises. |
| doi_str_mv | 10.1039/D2GC02311C |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2715888178</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2715888178</sourcerecordid><originalsourceid>FETCH-LOGICAL-c259t-60bd5b423f7caae588bd7c2f7d1a38cef841ad8cd474b66ee35ec3b0e30e0d7f3</originalsourceid><addsrcrecordid>eNpFkEtLAzEUhYMoWKsbf0HAnTCax8wkXcpoq1DQha6HPG7alHZSkwx0_r1TKrq6B-7HOYeD0C0lD5Tw2eMzWzSEcUqbMzShZc2LGRPk_E_X7BJdpbQhhFJRlxP0MfcH362wUVGHDlsfDt4C9h1OPvfY9vH4hbxWXdjifQy2N9mPpB6wC3GnMmAL68HGsIJOJbhGF05tE9z83in6mr98Nq_F8n3x1jwtC8OqWS5qom2lS8adMEpBJaW2wjAnLFVcGnCypMpKY0tR6roG4BUYrglwAsQKx6fo7uQ7dvruIeV2E_rYjZEtE3T0k1TIkbo_USaGlCK4dh_9TsWhpaQ9Ltb-L8Z_AMOfXzc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2715888178</pqid></control><display><type>article</type><title>Fixing carbon dioxide in situ during ethanol production by formate dehydrogenase</title><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Du, Cong ; Li, Yimin ; he, Ying ; Su, Liming ; Wang, Huanan ; Yuan, Wenjie ; Bai, Fengwu</creator><creatorcontrib>Du, Cong ; Li, Yimin ; he, Ying ; Su, Liming ; Wang, Huanan ; Yuan, Wenjie ; Bai, Fengwu</creatorcontrib><description>Concerns regarding the increasing carbon dioxide (CO
2
) concentration has aroused global interest in renewable fuels and conversion of CO
2
into chemicals. This study provides a sustainable strategy for fixing CO
2
in situ
during the production of ethanol in a single cell expressing formate dehydrogenase (FDH) without the addition of a cofactor. Glucose was converted to ethanol, with the main by-product CO
2
fixed to formate, increasing the yield of ethanol and glucose fermentation efficiency in the recombinant
Saccharomyces cerevisiae-fdh
. Higher concentrations of glucose and amount of ScFDH1 yielded more formate, confirming the feasibility of CO
2
fixation. Significantly, the glucose consumption rate and ethanol yield increased by 30% and 13% after the overexpression of ScFDH1, respectively. Finally, the increase in CO
2
concentration with the addition of zeolite imidazole framework-8 (ZIF-8) further demonstrated the CO
2
fixation
in situ
, increasing the production of formate 2.1 times. Due to ZIF-8, up to 60.67 mg L
−1
formate was produced. Our findings establish a green, economical, and sustainable alternative for CO
2
conversion
in situ
, contributing to the ethanol yield by ScFDH1 without extra energy input and cofactor supply. This synergistic strategy has significance for both the energy and environmental crises.</description><identifier>ISSN: 1463-9262</identifier><identifier>EISSN: 1463-9270</identifier><identifier>DOI: 10.1039/D2GC02311C</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Carbon dioxide ; Carbon dioxide concentration ; Carbon dioxide fixation ; Conversion ; Dehydrogenase ; Dehydrogenases ; Ethanol ; Fermentation ; Fixation ; Fixing ; Formate dehydrogenase ; Glucose ; Green chemistry ; Imidazole ; Zeolites</subject><ispartof>Green chemistry : an international journal and green chemistry resource : GC, 2022-09, Vol.24 (18), p.6989-6999</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c259t-60bd5b423f7caae588bd7c2f7d1a38cef841ad8cd474b66ee35ec3b0e30e0d7f3</citedby><cites>FETCH-LOGICAL-c259t-60bd5b423f7caae588bd7c2f7d1a38cef841ad8cd474b66ee35ec3b0e30e0d7f3</cites><orcidid>0000-0002-0461-4689 ; 0000-0003-3950-7397 ; 0000-0002-1228-2514 ; 0000-0002-7232-267X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Du, Cong</creatorcontrib><creatorcontrib>Li, Yimin</creatorcontrib><creatorcontrib>he, Ying</creatorcontrib><creatorcontrib>Su, Liming</creatorcontrib><creatorcontrib>Wang, Huanan</creatorcontrib><creatorcontrib>Yuan, Wenjie</creatorcontrib><creatorcontrib>Bai, Fengwu</creatorcontrib><title>Fixing carbon dioxide in situ during ethanol production by formate dehydrogenase</title><title>Green chemistry : an international journal and green chemistry resource : GC</title><description>Concerns regarding the increasing carbon dioxide (CO
2
) concentration has aroused global interest in renewable fuels and conversion of CO
2
into chemicals. This study provides a sustainable strategy for fixing CO
2
in situ
during the production of ethanol in a single cell expressing formate dehydrogenase (FDH) without the addition of a cofactor. Glucose was converted to ethanol, with the main by-product CO
2
fixed to formate, increasing the yield of ethanol and glucose fermentation efficiency in the recombinant
Saccharomyces cerevisiae-fdh
. Higher concentrations of glucose and amount of ScFDH1 yielded more formate, confirming the feasibility of CO
2
fixation. Significantly, the glucose consumption rate and ethanol yield increased by 30% and 13% after the overexpression of ScFDH1, respectively. Finally, the increase in CO
2
concentration with the addition of zeolite imidazole framework-8 (ZIF-8) further demonstrated the CO
2
fixation
in situ
, increasing the production of formate 2.1 times. Due to ZIF-8, up to 60.67 mg L
−1
formate was produced. Our findings establish a green, economical, and sustainable alternative for CO
2
conversion
in situ
, contributing to the ethanol yield by ScFDH1 without extra energy input and cofactor supply. This synergistic strategy has significance for both the energy and environmental crises.</description><subject>Carbon dioxide</subject><subject>Carbon dioxide concentration</subject><subject>Carbon dioxide fixation</subject><subject>Conversion</subject><subject>Dehydrogenase</subject><subject>Dehydrogenases</subject><subject>Ethanol</subject><subject>Fermentation</subject><subject>Fixation</subject><subject>Fixing</subject><subject>Formate dehydrogenase</subject><subject>Glucose</subject><subject>Green chemistry</subject><subject>Imidazole</subject><subject>Zeolites</subject><issn>1463-9262</issn><issn>1463-9270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpFkEtLAzEUhYMoWKsbf0HAnTCax8wkXcpoq1DQha6HPG7alHZSkwx0_r1TKrq6B-7HOYeD0C0lD5Tw2eMzWzSEcUqbMzShZc2LGRPk_E_X7BJdpbQhhFJRlxP0MfcH362wUVGHDlsfDt4C9h1OPvfY9vH4hbxWXdjifQy2N9mPpB6wC3GnMmAL68HGsIJOJbhGF05tE9z83in6mr98Nq_F8n3x1jwtC8OqWS5qom2lS8adMEpBJaW2wjAnLFVcGnCypMpKY0tR6roG4BUYrglwAsQKx6fo7uQ7dvruIeV2E_rYjZEtE3T0k1TIkbo_USaGlCK4dh_9TsWhpaQ9Ltb-L8Z_AMOfXzc</recordid><startdate>20220920</startdate><enddate>20220920</enddate><creator>Du, Cong</creator><creator>Li, Yimin</creator><creator>he, Ying</creator><creator>Su, Liming</creator><creator>Wang, Huanan</creator><creator>Yuan, Wenjie</creator><creator>Bai, Fengwu</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7ST</scope><scope>7U6</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>JG9</scope><orcidid>https://orcid.org/0000-0002-0461-4689</orcidid><orcidid>https://orcid.org/0000-0003-3950-7397</orcidid><orcidid>https://orcid.org/0000-0002-1228-2514</orcidid><orcidid>https://orcid.org/0000-0002-7232-267X</orcidid></search><sort><creationdate>20220920</creationdate><title>Fixing carbon dioxide in situ during ethanol production by formate dehydrogenase</title><author>Du, Cong ; Li, Yimin ; he, Ying ; Su, Liming ; Wang, Huanan ; Yuan, Wenjie ; Bai, Fengwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c259t-60bd5b423f7caae588bd7c2f7d1a38cef841ad8cd474b66ee35ec3b0e30e0d7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Carbon dioxide</topic><topic>Carbon dioxide concentration</topic><topic>Carbon dioxide fixation</topic><topic>Conversion</topic><topic>Dehydrogenase</topic><topic>Dehydrogenases</topic><topic>Ethanol</topic><topic>Fermentation</topic><topic>Fixation</topic><topic>Fixing</topic><topic>Formate dehydrogenase</topic><topic>Glucose</topic><topic>Green chemistry</topic><topic>Imidazole</topic><topic>Zeolites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Cong</creatorcontrib><creatorcontrib>Li, Yimin</creatorcontrib><creatorcontrib>he, Ying</creatorcontrib><creatorcontrib>Su, Liming</creatorcontrib><creatorcontrib>Wang, Huanan</creatorcontrib><creatorcontrib>Yuan, Wenjie</creatorcontrib><creatorcontrib>Bai, Fengwu</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Sustainability Science Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Materials Research Database</collection><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Cong</au><au>Li, Yimin</au><au>he, Ying</au><au>Su, Liming</au><au>Wang, Huanan</au><au>Yuan, Wenjie</au><au>Bai, Fengwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fixing carbon dioxide in situ during ethanol production by formate dehydrogenase</atitle><jtitle>Green chemistry : an international journal and green chemistry resource : GC</jtitle><date>2022-09-20</date><risdate>2022</risdate><volume>24</volume><issue>18</issue><spage>6989</spage><epage>6999</epage><pages>6989-6999</pages><issn>1463-9262</issn><eissn>1463-9270</eissn><abstract>Concerns regarding the increasing carbon dioxide (CO
2
) concentration has aroused global interest in renewable fuels and conversion of CO
2
into chemicals. This study provides a sustainable strategy for fixing CO
2
in situ
during the production of ethanol in a single cell expressing formate dehydrogenase (FDH) without the addition of a cofactor. Glucose was converted to ethanol, with the main by-product CO
2
fixed to formate, increasing the yield of ethanol and glucose fermentation efficiency in the recombinant
Saccharomyces cerevisiae-fdh
. Higher concentrations of glucose and amount of ScFDH1 yielded more formate, confirming the feasibility of CO
2
fixation. Significantly, the glucose consumption rate and ethanol yield increased by 30% and 13% after the overexpression of ScFDH1, respectively. Finally, the increase in CO
2
concentration with the addition of zeolite imidazole framework-8 (ZIF-8) further demonstrated the CO
2
fixation
in situ
, increasing the production of formate 2.1 times. Due to ZIF-8, up to 60.67 mg L
−1
formate was produced. Our findings establish a green, economical, and sustainable alternative for CO
2
conversion
in situ
, contributing to the ethanol yield by ScFDH1 without extra energy input and cofactor supply. This synergistic strategy has significance for both the energy and environmental crises.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/D2GC02311C</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-0461-4689</orcidid><orcidid>https://orcid.org/0000-0003-3950-7397</orcidid><orcidid>https://orcid.org/0000-0002-1228-2514</orcidid><orcidid>https://orcid.org/0000-0002-7232-267X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1463-9262 |
| ispartof | Green chemistry : an international journal and green chemistry resource : GC, 2022-09, Vol.24 (18), p.6989-6999 |
| issn | 1463-9262 1463-9270 |
| language | eng |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Carbon dioxide Carbon dioxide concentration Carbon dioxide fixation Conversion Dehydrogenase Dehydrogenases Ethanol Fermentation Fixation Fixing Formate dehydrogenase Glucose Green chemistry Imidazole Zeolites |
| title | Fixing carbon dioxide in situ during ethanol production by formate dehydrogenase |
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