Anti‐inflammatory effect of Bifidobacterium animalis subsp. lactis A6 on DSS‐induced colitis in mice
Aims This study aimed to investigate the protective effect of Bifidobacterium animalis subsp. lactis A6 on dextran sodium sulphate (DSS)‐induced colitis in C57BL/6J mice. Methods and Results Mice were randomly divided into three groups (n = 8 per group). Each group was administered with PBS (Control...
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| Veröffentlicht in: | Journal of applied microbiology 2022-09, Vol.133 (3), p.2063-2073 |
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| container_title | Journal of applied microbiology |
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| creator | Wang, Hui Fan, Chengfei Zhao, Zhaoer Zhai, Zhengyuan Hao, Yanling |
| description | Aims
This study aimed to investigate the protective effect of Bifidobacterium animalis subsp. lactis A6 on dextran sodium sulphate (DSS)‐induced colitis in C57BL/6J mice.
Methods and Results
Mice were randomly divided into three groups (n = 8 per group). Each group was administered with PBS (Control and DSS group) or B. lactis A6 with a dosage of ~4.0 × 109 CFU day−1 (DSS + A6 group) for 21 consecutive days. The DSS and DSS + A6 group mice were ad libitum drinking 2.5% DSS water during day 15–21, while the Control group mice were given normal water. The administration of B. lactis A6 significantly inhibited DSS‐induced bodyweight loss and colon shortening (p 0.05). The intestinal barrier integrity was improved by reducing colonic damage, recovering mucus layer loss and enhancing tight junction expression including ZO‐1, occludin and claudin‐1. In addition, B. lactis A6 attenuated the oxidative stress by decreasing MDA and increasing SOD and GSH levels in colon tissues. Moreover, B. lactis A6 suppressed DSS‐induced inflammatory responses via downregulating TNF‐α, IL‐1β and IL‐6 levels and upregulating IL‐10 level in colon tissues.
Conclusion
B. lactis A6 effectively alleviated DSS‐induced colitis by maintaining intestinal barrier integrity, reducing oxidative stress and inhibiting inflammatory responses.
Significance and Impact of the Study
This study suggests that B. lactis A6 could act as a candidate probiotic for UC treatment. |
| doi_str_mv | 10.1111/jam.15681 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2709886315</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2709886315</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3301-8effaded4e6351593112f432ab90c50f1b29204b1b67a3fe891122f4af204cf33</originalsourceid><addsrcrecordid>eNp1kEtOwzAQhi0EEqWw4AaWWLFI60fsJMtQyktFLApry3FsyVUSFzsR6o4jcATOwlE4CaZhy2xmNPPNP5ofgHOMZjjGfCPbGWY8xwdggilnCeEZOdzXacJQRo7BSQgbhDBFjE-ALbvefr9_2M40sm1l7_wOamO06qEz8MoaW7tKql57O7RQdraVjQ0wDFXYzmATJzZ8fZYcug5er9d7qXpQuobKNTYOoe1ga5U-BUdGNkGf_eUpeLlZPi_uktXT7f2iXCWKUoSTPB6Xta5TzSnDrKAYE5NSIqsCKYYMrkhBUFrhimeSGp0XEYiENLGrDKVTcDHqbr17HXToxcYNvosnBclQkeecYhapy5FS3oXgtRFbH1_zO4GR-HVSRCfF3snIzkf2zTZ69z8oHsrHceMHrGJ3dg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2709886315</pqid></control><display><type>article</type><title>Anti‐inflammatory effect of Bifidobacterium animalis subsp. lactis A6 on DSS‐induced colitis in mice</title><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Wang, Hui ; Fan, Chengfei ; Zhao, Zhaoer ; Zhai, Zhengyuan ; Hao, Yanling</creator><creatorcontrib>Wang, Hui ; Fan, Chengfei ; Zhao, Zhaoer ; Zhai, Zhengyuan ; Hao, Yanling</creatorcontrib><description>Aims
This study aimed to investigate the protective effect of Bifidobacterium animalis subsp. lactis A6 on dextran sodium sulphate (DSS)‐induced colitis in C57BL/6J mice.
Methods and Results
Mice were randomly divided into three groups (n = 8 per group). Each group was administered with PBS (Control and DSS group) or B. lactis A6 with a dosage of ~4.0 × 109 CFU day−1 (DSS + A6 group) for 21 consecutive days. The DSS and DSS + A6 group mice were ad libitum drinking 2.5% DSS water during day 15–21, while the Control group mice were given normal water. The administration of B. lactis A6 significantly inhibited DSS‐induced bodyweight loss and colon shortening (p < 0.001), but showed no significant influence on the spleen enlargement (p > 0.05). The intestinal barrier integrity was improved by reducing colonic damage, recovering mucus layer loss and enhancing tight junction expression including ZO‐1, occludin and claudin‐1. In addition, B. lactis A6 attenuated the oxidative stress by decreasing MDA and increasing SOD and GSH levels in colon tissues. Moreover, B. lactis A6 suppressed DSS‐induced inflammatory responses via downregulating TNF‐α, IL‐1β and IL‐6 levels and upregulating IL‐10 level in colon tissues.
Conclusion
B. lactis A6 effectively alleviated DSS‐induced colitis by maintaining intestinal barrier integrity, reducing oxidative stress and inhibiting inflammatory responses.
Significance and Impact of the Study
This study suggests that B. lactis A6 could act as a candidate probiotic for UC treatment.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.15681</identifier><language>eng</language><publisher>Cambridge: Oxford University Press</publisher><subject>Bifidobacterium animalis ; Bifidobacterium animalis subsp. lactis A6 ; Colitis ; Colon ; Dextran ; Dextrans ; Drinking water ; DSS‐induced colitis ; Inflammation ; Inflammatory bowel disease ; inflammatory responses ; Integrity ; Interleukin 6 ; intestinal barrier integrity ; Intestine ; Oxidative stress ; Probiotics ; Sodium sulfate ; Tumor necrosis factor</subject><ispartof>Journal of applied microbiology, 2022-09, Vol.133 (3), p.2063-2073</ispartof><rights>2022 The Society for Applied Microbiology.</rights><rights>Copyright © 2022 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3301-8effaded4e6351593112f432ab90c50f1b29204b1b67a3fe891122f4af204cf33</citedby><cites>FETCH-LOGICAL-c3301-8effaded4e6351593112f432ab90c50f1b29204b1b67a3fe891122f4af204cf33</cites><orcidid>0000-0002-2562-6849</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.15681$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.15681$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Fan, Chengfei</creatorcontrib><creatorcontrib>Zhao, Zhaoer</creatorcontrib><creatorcontrib>Zhai, Zhengyuan</creatorcontrib><creatorcontrib>Hao, Yanling</creatorcontrib><title>Anti‐inflammatory effect of Bifidobacterium animalis subsp. lactis A6 on DSS‐induced colitis in mice</title><title>Journal of applied microbiology</title><description>Aims
This study aimed to investigate the protective effect of Bifidobacterium animalis subsp. lactis A6 on dextran sodium sulphate (DSS)‐induced colitis in C57BL/6J mice.
Methods and Results
Mice were randomly divided into three groups (n = 8 per group). Each group was administered with PBS (Control and DSS group) or B. lactis A6 with a dosage of ~4.0 × 109 CFU day−1 (DSS + A6 group) for 21 consecutive days. The DSS and DSS + A6 group mice were ad libitum drinking 2.5% DSS water during day 15–21, while the Control group mice were given normal water. The administration of B. lactis A6 significantly inhibited DSS‐induced bodyweight loss and colon shortening (p < 0.001), but showed no significant influence on the spleen enlargement (p > 0.05). The intestinal barrier integrity was improved by reducing colonic damage, recovering mucus layer loss and enhancing tight junction expression including ZO‐1, occludin and claudin‐1. In addition, B. lactis A6 attenuated the oxidative stress by decreasing MDA and increasing SOD and GSH levels in colon tissues. Moreover, B. lactis A6 suppressed DSS‐induced inflammatory responses via downregulating TNF‐α, IL‐1β and IL‐6 levels and upregulating IL‐10 level in colon tissues.
Conclusion
B. lactis A6 effectively alleviated DSS‐induced colitis by maintaining intestinal barrier integrity, reducing oxidative stress and inhibiting inflammatory responses.
Significance and Impact of the Study
This study suggests that B. lactis A6 could act as a candidate probiotic for UC treatment.</description><subject>Bifidobacterium animalis</subject><subject>Bifidobacterium animalis subsp. lactis A6</subject><subject>Colitis</subject><subject>Colon</subject><subject>Dextran</subject><subject>Dextrans</subject><subject>Drinking water</subject><subject>DSS‐induced colitis</subject><subject>Inflammation</subject><subject>Inflammatory bowel disease</subject><subject>inflammatory responses</subject><subject>Integrity</subject><subject>Interleukin 6</subject><subject>intestinal barrier integrity</subject><subject>Intestine</subject><subject>Oxidative stress</subject><subject>Probiotics</subject><subject>Sodium sulfate</subject><subject>Tumor necrosis factor</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kEtOwzAQhi0EEqWw4AaWWLFI60fsJMtQyktFLApry3FsyVUSFzsR6o4jcATOwlE4CaZhy2xmNPPNP5ofgHOMZjjGfCPbGWY8xwdggilnCeEZOdzXacJQRo7BSQgbhDBFjE-ALbvefr9_2M40sm1l7_wOamO06qEz8MoaW7tKql57O7RQdraVjQ0wDFXYzmATJzZ8fZYcug5er9d7qXpQuobKNTYOoe1ga5U-BUdGNkGf_eUpeLlZPi_uktXT7f2iXCWKUoSTPB6Xta5TzSnDrKAYE5NSIqsCKYYMrkhBUFrhimeSGp0XEYiENLGrDKVTcDHqbr17HXToxcYNvosnBclQkeecYhapy5FS3oXgtRFbH1_zO4GR-HVSRCfF3snIzkf2zTZ69z8oHsrHceMHrGJ3dg</recordid><startdate>202209</startdate><enddate>202209</enddate><creator>Wang, Hui</creator><creator>Fan, Chengfei</creator><creator>Zhao, Zhaoer</creator><creator>Zhai, Zhengyuan</creator><creator>Hao, Yanling</creator><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0002-2562-6849</orcidid></search><sort><creationdate>202209</creationdate><title>Anti‐inflammatory effect of Bifidobacterium animalis subsp. lactis A6 on DSS‐induced colitis in mice</title><author>Wang, Hui ; Fan, Chengfei ; Zhao, Zhaoer ; Zhai, Zhengyuan ; Hao, Yanling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3301-8effaded4e6351593112f432ab90c50f1b29204b1b67a3fe891122f4af204cf33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bifidobacterium animalis</topic><topic>Bifidobacterium animalis subsp. lactis A6</topic><topic>Colitis</topic><topic>Colon</topic><topic>Dextran</topic><topic>Dextrans</topic><topic>Drinking water</topic><topic>DSS‐induced colitis</topic><topic>Inflammation</topic><topic>Inflammatory bowel disease</topic><topic>inflammatory responses</topic><topic>Integrity</topic><topic>Interleukin 6</topic><topic>intestinal barrier integrity</topic><topic>Intestine</topic><topic>Oxidative stress</topic><topic>Probiotics</topic><topic>Sodium sulfate</topic><topic>Tumor necrosis factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Hui</creatorcontrib><creatorcontrib>Fan, Chengfei</creatorcontrib><creatorcontrib>Zhao, Zhaoer</creatorcontrib><creatorcontrib>Zhai, Zhengyuan</creatorcontrib><creatorcontrib>Hao, Yanling</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Hui</au><au>Fan, Chengfei</au><au>Zhao, Zhaoer</au><au>Zhai, Zhengyuan</au><au>Hao, Yanling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti‐inflammatory effect of Bifidobacterium animalis subsp. lactis A6 on DSS‐induced colitis in mice</atitle><jtitle>Journal of applied microbiology</jtitle><date>2022-09</date><risdate>2022</risdate><volume>133</volume><issue>3</issue><spage>2063</spage><epage>2073</epage><pages>2063-2073</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
This study aimed to investigate the protective effect of Bifidobacterium animalis subsp. lactis A6 on dextran sodium sulphate (DSS)‐induced colitis in C57BL/6J mice.
Methods and Results
Mice were randomly divided into three groups (n = 8 per group). Each group was administered with PBS (Control and DSS group) or B. lactis A6 with a dosage of ~4.0 × 109 CFU day−1 (DSS + A6 group) for 21 consecutive days. The DSS and DSS + A6 group mice were ad libitum drinking 2.5% DSS water during day 15–21, while the Control group mice were given normal water. The administration of B. lactis A6 significantly inhibited DSS‐induced bodyweight loss and colon shortening (p < 0.001), but showed no significant influence on the spleen enlargement (p > 0.05). The intestinal barrier integrity was improved by reducing colonic damage, recovering mucus layer loss and enhancing tight junction expression including ZO‐1, occludin and claudin‐1. In addition, B. lactis A6 attenuated the oxidative stress by decreasing MDA and increasing SOD and GSH levels in colon tissues. Moreover, B. lactis A6 suppressed DSS‐induced inflammatory responses via downregulating TNF‐α, IL‐1β and IL‐6 levels and upregulating IL‐10 level in colon tissues.
Conclusion
B. lactis A6 effectively alleviated DSS‐induced colitis by maintaining intestinal barrier integrity, reducing oxidative stress and inhibiting inflammatory responses.
Significance and Impact of the Study
This study suggests that B. lactis A6 could act as a candidate probiotic for UC treatment.</abstract><cop>Cambridge</cop><pub>Oxford University Press</pub><doi>10.1111/jam.15681</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2562-6849</orcidid></addata></record> |
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| subjects | Bifidobacterium animalis Bifidobacterium animalis subsp. lactis A6 Colitis Colon Dextran Dextrans Drinking water DSS‐induced colitis Inflammation Inflammatory bowel disease inflammatory responses Integrity Interleukin 6 intestinal barrier integrity Intestine Oxidative stress Probiotics Sodium sulfate Tumor necrosis factor |
| title | Anti‐inflammatory effect of Bifidobacterium animalis subsp. lactis A6 on DSS‐induced colitis in mice |
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