Development of Small-sized Lysine Enzyme Sensor for Clinical Use
We have developed an electrochemical biosensor to measure the lysine concentration in blood. The lysine biosensor has a simple design and can complete measurements of a 5 μL sample in 2 min, enabling rapid measurement in clinical practice. The target molecules are catalyzed by enzymes encapsulated i...
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| Veröffentlicht in: | Sensors and materials 2022-01, Vol.34 (8), p.3173 |
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| container_title | Sensors and materials |
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| creator | Uemura, Takeshi Fujii, Shunsuke Yamazaki, Hiroki Itoh, Tetsuji Masumoto, Kouji Nishizawa, Seiichi |
| description | We have developed an electrochemical biosensor to measure the lysine concentration in blood. The lysine biosensor has a simple design and can complete measurements of a 5 μL sample in 2 min, enabling rapid measurement in clinical practice. The target molecules are catalyzed by enzymes encapsulated in carboxymethyl cellulose on the working electrodes to produce potassium ferrocyanide as an electron mediator that is detected on the electrodes amperometrically. In a clinical application, the sensor was used to measure blood lysine concentrations in 47 pediatric surgical patients. The results showed that the lysine level was correlated with the transthyretin level, a conventional nutritional indicator, confirming its usefulness for nutritional assessment. This sensor can be used for postoperative nutritional management and treatment of rare diseases such as lysinuric protein intolerance. The platform also has a versatile design for the detection of other amino acids in addition to lysine. |
| doi_str_mv | 10.18494/SAM3914 |
| format | Article |
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The lysine biosensor has a simple design and can complete measurements of a 5 μL sample in 2 min, enabling rapid measurement in clinical practice. The target molecules are catalyzed by enzymes encapsulated in carboxymethyl cellulose on the working electrodes to produce potassium ferrocyanide as an electron mediator that is detected on the electrodes amperometrically. In a clinical application, the sensor was used to measure blood lysine concentrations in 47 pediatric surgical patients. The results showed that the lysine level was correlated with the transthyretin level, a conventional nutritional indicator, confirming its usefulness for nutritional assessment. This sensor can be used for postoperative nutritional management and treatment of rare diseases such as lysinuric protein intolerance. The platform also has a versatile design for the detection of other amino acids in addition to lysine.</description><identifier>ISSN: 0914-4935</identifier><identifier>EISSN: 2435-0869</identifier><identifier>DOI: 10.18494/SAM3914</identifier><language>eng</language><publisher>Tokyo: MYU Scientific Publishing Division</publisher><subject>Amino acids ; Biosensors ; Blood ; Carboxymethyl cellulose ; Electrical measurement ; Electrodes ; Iron cyanides ; Lysine</subject><ispartof>Sensors and materials, 2022-01, Vol.34 (8), p.3173</ispartof><rights>Copyright MYU Scientific Publishing Division 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27903,27904</link.rule.ids></links><search><creatorcontrib>Uemura, Takeshi</creatorcontrib><creatorcontrib>Fujii, Shunsuke</creatorcontrib><creatorcontrib>Yamazaki, Hiroki</creatorcontrib><creatorcontrib>Itoh, Tetsuji</creatorcontrib><creatorcontrib>Masumoto, Kouji</creatorcontrib><creatorcontrib>Nishizawa, Seiichi</creatorcontrib><title>Development of Small-sized Lysine Enzyme Sensor for Clinical Use</title><title>Sensors and materials</title><description>We have developed an electrochemical biosensor to measure the lysine concentration in blood. 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The platform also has a versatile design for the detection of other amino acids in addition to lysine.</description><subject>Amino acids</subject><subject>Biosensors</subject><subject>Blood</subject><subject>Carboxymethyl cellulose</subject><subject>Electrical measurement</subject><subject>Electrodes</subject><subject>Iron cyanides</subject><subject>Lysine</subject><issn>0914-4935</issn><issn>2435-0869</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNotUE1Lw0AUXETBUAv-hAUvXqJvsx_J3iy1VaHiIfYcNtm3kJKPupsK7a93sT28eTDMmzcMIfcMnlghtHguF59cM3FFkkxwmUKh9DVJIFKp0FzeknkIOwBghQSVqYS8vOIvduO-x2Gio6Nlb7ouDe0JLd0cQzsgXQ2nY4-0xCGMnro4y64d2sZ0dBvwjtw40wWcX_aMbNer7-V7uvl6-1guNmnDmZpS4yCvlWDG1rU0qEFENHltiwyMRamsNjGyyZWWubO8cFbphmE80KzONZ-Rh7Pv3o8_BwxTtRsPfogvqywHwUEUTEbV41nV-DEEj67a-7Y3_lgxqP4rqi4V8T_NQlfP</recordid><startdate>20220101</startdate><enddate>20220101</enddate><creator>Uemura, Takeshi</creator><creator>Fujii, Shunsuke</creator><creator>Yamazaki, Hiroki</creator><creator>Itoh, Tetsuji</creator><creator>Masumoto, Kouji</creator><creator>Nishizawa, Seiichi</creator><general>MYU Scientific Publishing Division</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20220101</creationdate><title>Development of Small-sized Lysine Enzyme Sensor for Clinical Use</title><author>Uemura, Takeshi ; Fujii, Shunsuke ; Yamazaki, Hiroki ; Itoh, Tetsuji ; Masumoto, Kouji ; Nishizawa, Seiichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-af07b641adbb5ae9045aea7bd820ade56d9a243a76957fd38fd69c1e1ad91b793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amino acids</topic><topic>Biosensors</topic><topic>Blood</topic><topic>Carboxymethyl cellulose</topic><topic>Electrical measurement</topic><topic>Electrodes</topic><topic>Iron cyanides</topic><topic>Lysine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uemura, Takeshi</creatorcontrib><creatorcontrib>Fujii, Shunsuke</creatorcontrib><creatorcontrib>Yamazaki, Hiroki</creatorcontrib><creatorcontrib>Itoh, Tetsuji</creatorcontrib><creatorcontrib>Masumoto, Kouji</creatorcontrib><creatorcontrib>Nishizawa, Seiichi</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uemura, Takeshi</au><au>Fujii, Shunsuke</au><au>Yamazaki, Hiroki</au><au>Itoh, Tetsuji</au><au>Masumoto, Kouji</au><au>Nishizawa, Seiichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Small-sized Lysine Enzyme Sensor for Clinical Use</atitle><jtitle>Sensors and materials</jtitle><date>2022-01-01</date><risdate>2022</risdate><volume>34</volume><issue>8</issue><spage>3173</spage><pages>3173-</pages><issn>0914-4935</issn><eissn>2435-0869</eissn><abstract>We have developed an electrochemical biosensor to measure the lysine concentration in blood. 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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino acids Biosensors Blood Carboxymethyl cellulose Electrical measurement Electrodes Iron cyanides Lysine |
| title | Development of Small-sized Lysine Enzyme Sensor for Clinical Use |
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