Morin-loaded nanoceria as an efficient nanoformulation for increased antioxidant and antibacterial efficacy
Nanoparticles (NPs) are widely utilized as an effective carrier for naturally occurring biologically active hydrophobic molecules with a potential to enhance antioxidant and antibacterial activities. Here, a positively charged amine-functionalized ceria nanoparticles (CeO 2 -NH 2 NPs) were prepared...
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| Veröffentlicht in: | Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology 2022-09, Vol.24 (9), Article 176 |
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| creator | Thakur, Neelam Kundu, Mousumi Chatterjee, Sharmistha Singh, Th. Abhishek Das, Joydeep Sil, Parames C. |
| description | Nanoparticles (NPs) are widely utilized as an effective carrier for naturally occurring biologically active hydrophobic molecules with a potential to enhance antioxidant and antibacterial activities. Here, a positively charged amine-functionalized ceria nanoparticles (CeO
2
-NH
2
NPs) were prepared first, and then a natural flavonoid, morin was adsorbed on the surface of NPs via metal–ligand coordination to form a nanohybrid (morin-CeO
2
-NH
2
). The CeO
2
-NH
2
NPs displayed almost spherical shape with size of around 3–4 nm, along with the presence of Ce, O, N, C, and Si. The NPs showed ~ 10% drug (morin) loading content, and a sustained release pattern of morin under physiological conditions. The nanohybrids showed excellent free radical (DPPH, superoxide, and hydrogen peroxide) scavenging properties in both cell-free systems as well as in intracellular conditions. Cytotoxicity assessment revealed that the nanohybrids were slightly toxic up to the concentration of 50 μg/mL in normal human kidney epithelial cells. Further, the nanohybrids showed a significant growth inhibition against the tested Gram-negative as well as Gram-positive bacteria due to the production of excessive reactive oxygen species (ROS), DNA degradation, and membrane damage. However, those in vitro assays showed that the nanohybrid exhibited the maximum effect in imparting the antioxidant and antibacterial activity among all the treatment groups. All these results highlighted that the nanoceria-mediated drug delivery systems could help to increase the antioxidant and antibacterial potential of morin.
Graphical abstract |
| doi_str_mv | 10.1007/s11051-022-05552-x |
| format | Article |
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2
-NH
2
NPs) were prepared first, and then a natural flavonoid, morin was adsorbed on the surface of NPs via metal–ligand coordination to form a nanohybrid (morin-CeO
2
-NH
2
). The CeO
2
-NH
2
NPs displayed almost spherical shape with size of around 3–4 nm, along with the presence of Ce, O, N, C, and Si. The NPs showed ~ 10% drug (morin) loading content, and a sustained release pattern of morin under physiological conditions. The nanohybrids showed excellent free radical (DPPH, superoxide, and hydrogen peroxide) scavenging properties in both cell-free systems as well as in intracellular conditions. Cytotoxicity assessment revealed that the nanohybrids were slightly toxic up to the concentration of 50 μg/mL in normal human kidney epithelial cells. Further, the nanohybrids showed a significant growth inhibition against the tested Gram-negative as well as Gram-positive bacteria due to the production of excessive reactive oxygen species (ROS), DNA degradation, and membrane damage. However, those in vitro assays showed that the nanohybrid exhibited the maximum effect in imparting the antioxidant and antibacterial activity among all the treatment groups. All these results highlighted that the nanoceria-mediated drug delivery systems could help to increase the antioxidant and antibacterial potential of morin.
Graphical abstract</description><identifier>ISSN: 1388-0764</identifier><identifier>EISSN: 1572-896X</identifier><identifier>DOI: 10.1007/s11051-022-05552-x</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Antibacterial activity ; Antioxidants ; Bacteria ; Biological activity ; Cerium oxides ; Characterization and Evaluation of Materials ; Chemistry and Materials Science ; Controlled release ; Cytotoxicity ; DNA damage ; Drug delivery ; Drug delivery systems ; Epithelial cells ; Epithelium ; Flavonoids ; Free radicals ; Gram-positive bacteria ; Hydrogen peroxide ; Hydrophobicity ; Inorganic Chemistry ; Lasers ; Materials Science ; Nanoparticles ; Nanotechnology ; Optical Devices ; Optics ; Photonics ; Physical Chemistry ; Reactive oxygen species ; Research Paper ; Scavenging ; Sustained release ; Toxicity</subject><ispartof>Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology, 2022-09, Vol.24 (9), Article 176</ispartof><rights>The Author(s), under exclusive licence to Springer Nature B.V. 2022. Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-753472a7de7301721823bbdbe06c6d0fcca65a68946a307a7ce0407bc1b0c8343</citedby><cites>FETCH-LOGICAL-c319t-753472a7de7301721823bbdbe06c6d0fcca65a68946a307a7ce0407bc1b0c8343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11051-022-05552-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11051-022-05552-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Thakur, Neelam</creatorcontrib><creatorcontrib>Kundu, Mousumi</creatorcontrib><creatorcontrib>Chatterjee, Sharmistha</creatorcontrib><creatorcontrib>Singh, Th. Abhishek</creatorcontrib><creatorcontrib>Das, Joydeep</creatorcontrib><creatorcontrib>Sil, Parames C.</creatorcontrib><title>Morin-loaded nanoceria as an efficient nanoformulation for increased antioxidant and antibacterial efficacy</title><title>Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology</title><addtitle>J Nanopart Res</addtitle><description>Nanoparticles (NPs) are widely utilized as an effective carrier for naturally occurring biologically active hydrophobic molecules with a potential to enhance antioxidant and antibacterial activities. Here, a positively charged amine-functionalized ceria nanoparticles (CeO
2
-NH
2
NPs) were prepared first, and then a natural flavonoid, morin was adsorbed on the surface of NPs via metal–ligand coordination to form a nanohybrid (morin-CeO
2
-NH
2
). The CeO
2
-NH
2
NPs displayed almost spherical shape with size of around 3–4 nm, along with the presence of Ce, O, N, C, and Si. The NPs showed ~ 10% drug (morin) loading content, and a sustained release pattern of morin under physiological conditions. The nanohybrids showed excellent free radical (DPPH, superoxide, and hydrogen peroxide) scavenging properties in both cell-free systems as well as in intracellular conditions. Cytotoxicity assessment revealed that the nanohybrids were slightly toxic up to the concentration of 50 μg/mL in normal human kidney epithelial cells. Further, the nanohybrids showed a significant growth inhibition against the tested Gram-negative as well as Gram-positive bacteria due to the production of excessive reactive oxygen species (ROS), DNA degradation, and membrane damage. However, those in vitro assays showed that the nanohybrid exhibited the maximum effect in imparting the antioxidant and antibacterial activity among all the treatment groups. All these results highlighted that the nanoceria-mediated drug delivery systems could help to increase the antioxidant and antibacterial potential of morin.
Graphical abstract</description><subject>Antibacterial activity</subject><subject>Antioxidants</subject><subject>Bacteria</subject><subject>Biological activity</subject><subject>Cerium oxides</subject><subject>Characterization and Evaluation of Materials</subject><subject>Chemistry and Materials Science</subject><subject>Controlled release</subject><subject>Cytotoxicity</subject><subject>DNA damage</subject><subject>Drug delivery</subject><subject>Drug delivery systems</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Flavonoids</subject><subject>Free radicals</subject><subject>Gram-positive bacteria</subject><subject>Hydrogen peroxide</subject><subject>Hydrophobicity</subject><subject>Inorganic Chemistry</subject><subject>Lasers</subject><subject>Materials Science</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Photonics</subject><subject>Physical Chemistry</subject><subject>Reactive oxygen species</subject><subject>Research Paper</subject><subject>Scavenging</subject><subject>Sustained release</subject><subject>Toxicity</subject><issn>1388-0764</issn><issn>1572-896X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9UE1LxDAUDKLguvoHPBU8R1-SNmmPsvgFK14UvIXXNJWs3WRNurD7741bwZunmffezDwYQi4ZXDMAdZMYg4pR4JxCVVWc7o7IjFWK07qR78eZi7qmoGR5Ss5SWgEwyRs-I5_PITpPh4Cd7QqPPhgbHRaYCvSF7XtnnPXj4dKHuN4OOLrgi8wL5020mLIPfV7uXJcx82lu0Yw_UcOUgmZ_Tk56HJK9-MU5ebu_e1080uXLw9PidkmNYM1IVSVKxVF1VglgirOai7btWgvSyA56Y1BWKOumlChAoTIWSlCtYS2YWpRiTq6m3E0MX1ubRr0K2-jzS80VlIwL1bCs4pPKxJBStL3eRLfGuNcM9E-peipV51L1oVS9yyYxmVIW-w8b_6L_cX0DII18Rg</recordid><startdate>20220901</startdate><enddate>20220901</enddate><creator>Thakur, Neelam</creator><creator>Kundu, Mousumi</creator><creator>Chatterjee, Sharmistha</creator><creator>Singh, Th. 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Abhishek</au><au>Das, Joydeep</au><au>Sil, Parames C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morin-loaded nanoceria as an efficient nanoformulation for increased antioxidant and antibacterial efficacy</atitle><jtitle>Journal of nanoparticle research : an interdisciplinary forum for nanoscale science and technology</jtitle><stitle>J Nanopart Res</stitle><date>2022-09-01</date><risdate>2022</risdate><volume>24</volume><issue>9</issue><artnum>176</artnum><issn>1388-0764</issn><eissn>1572-896X</eissn><abstract>Nanoparticles (NPs) are widely utilized as an effective carrier for naturally occurring biologically active hydrophobic molecules with a potential to enhance antioxidant and antibacterial activities. Here, a positively charged amine-functionalized ceria nanoparticles (CeO
2
-NH
2
NPs) were prepared first, and then a natural flavonoid, morin was adsorbed on the surface of NPs via metal–ligand coordination to form a nanohybrid (morin-CeO
2
-NH
2
). The CeO
2
-NH
2
NPs displayed almost spherical shape with size of around 3–4 nm, along with the presence of Ce, O, N, C, and Si. The NPs showed ~ 10% drug (morin) loading content, and a sustained release pattern of morin under physiological conditions. The nanohybrids showed excellent free radical (DPPH, superoxide, and hydrogen peroxide) scavenging properties in both cell-free systems as well as in intracellular conditions. Cytotoxicity assessment revealed that the nanohybrids were slightly toxic up to the concentration of 50 μg/mL in normal human kidney epithelial cells. Further, the nanohybrids showed a significant growth inhibition against the tested Gram-negative as well as Gram-positive bacteria due to the production of excessive reactive oxygen species (ROS), DNA degradation, and membrane damage. However, those in vitro assays showed that the nanohybrid exhibited the maximum effect in imparting the antioxidant and antibacterial activity among all the treatment groups. All these results highlighted that the nanoceria-mediated drug delivery systems could help to increase the antioxidant and antibacterial potential of morin.
Graphical abstract</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11051-022-05552-x</doi></addata></record> |
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| subjects | Antibacterial activity Antioxidants Bacteria Biological activity Cerium oxides Characterization and Evaluation of Materials Chemistry and Materials Science Controlled release Cytotoxicity DNA damage Drug delivery Drug delivery systems Epithelial cells Epithelium Flavonoids Free radicals Gram-positive bacteria Hydrogen peroxide Hydrophobicity Inorganic Chemistry Lasers Materials Science Nanoparticles Nanotechnology Optical Devices Optics Photonics Physical Chemistry Reactive oxygen species Research Paper Scavenging Sustained release Toxicity |
| title | Morin-loaded nanoceria as an efficient nanoformulation for increased antioxidant and antibacterial efficacy |
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