Antibody dual-functionalisation enabled through a modular divinylpyrimidine disulfide rebridging strategy
Herein we report the development of a methodology for the dual-functionalisation of IgG antibodies. This is accomplished through the combination of disulfide rebridging divinylpyrimidine technology, with bicyclononyne and methylcyclopropene handles to facilitate sequential SPAAC and IEDDA reactions....
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2022-08, Vol.58 (67), p.941-944 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Hanby, Abigail R Walsh, Stephen J Counsell, Andrew J Ashman, Nicola Mortensen, Kim T Carroll, Jason S Spring, David R |
| description | Herein we report the development of a methodology for the dual-functionalisation of IgG antibodies. This is accomplished through the combination of disulfide rebridging divinylpyrimidine technology, with bicyclononyne and methylcyclopropene handles to facilitate sequential SPAAC and IEDDA reactions. Advantageously, the strategy does not require metal catalysis and avoids the need for purification between functionalisation steps.
A modular and metal-free chemical methodology for the synthesis of dual-functionalised antibody-drug conjugates. |
| doi_str_mv | 10.1039/d2cc02515a |
| format | Article |
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| ispartof | Chemical communications (Cambridge, England), 2022-08, Vol.58 (67), p.941-944 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Antibodies Catalysis Chemistry Disulfides Immunoglobulin G |
| title | Antibody dual-functionalisation enabled through a modular divinylpyrimidine disulfide rebridging strategy |
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