A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells
A cell-derived decellularized extracellular matrix (dECM) plays a vital role in controlling cell functions because of its similarity to the in vivo microenvironment. In the process of stem cell differentiation, the composition of the dECM is not constant but is dynamically remolded. However, there i...
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| Veröffentlicht in: | Journal of materials chemistry. B, Materials for biology and medicine Materials for biology and medicine, 2022-08, Vol.1 (32), p.6171-618 |
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| creator | Xu, Fei Zheng, Ziran Yao, Mianfeng Zhu, Feiya Shen, Ting Li, Jiang Zhu, Chao Yang, Tianru Shao, Mengying Wan, Zicheng Fang, Changyun |
| description | A cell-derived decellularized extracellular matrix (dECM) plays a vital role in controlling cell functions because of its similarity to the
in vivo
microenvironment. In the process of stem cell differentiation, the composition of the dECM is not constant but is dynamically remolded. However, there is little information regarding the dynamic regulation by the dECM of the osteogenic differentiation of stem cells. Herein, four types of stepwise dECMs (0, 7, 14, and 21 d-ECM) were prepared from bone marrow-derived mesenchymal stem cells (BMSCs) undergoing osteogenic differentiation for 0, 7, 14, and 21 days after decellularization.
In vitro
experiments were designed to study the regulation of BMSC osteogenesis by dECMs. The results showed that all the dECMs could support the activity and proliferation of BMSCs but had different effects on their osteogenic differentiation. The 14d-ECM promoted the osteogenesis of BMSCs significantly compared with the other dECMs. Proteomic analysis demonstrated that the composition of dECMs changed over time. The 14d ECM had higher amounts of collagen type IV alpha 2 chain (COL4A2) than the other dECMs. Furthermore, COL4A2 was obviously enriched in the activated focal adhesion kinase (FAK)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways. Thus, the 14d-ECM could promote the osteogenic differentiation of BMSCs, which might be related to the high content of COL4A2 in the 14d-ECM by activating the FAK/PI3K/AKT signaling pathways.
14d-ECM secreted by BMSCs promotes the osteogenic differentiation of BMSCs. The underlying mechanism may be related to COL4A2 in 14d-ECM
via
activation of the FAK/PI3K/AKT signaling pathway. |
| doi_str_mv | 10.1039/d2tb00721e |
| format | Article |
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in vivo
microenvironment. In the process of stem cell differentiation, the composition of the dECM is not constant but is dynamically remolded. However, there is little information regarding the dynamic regulation by the dECM of the osteogenic differentiation of stem cells. Herein, four types of stepwise dECMs (0, 7, 14, and 21 d-ECM) were prepared from bone marrow-derived mesenchymal stem cells (BMSCs) undergoing osteogenic differentiation for 0, 7, 14, and 21 days after decellularization.
In vitro
experiments were designed to study the regulation of BMSC osteogenesis by dECMs. The results showed that all the dECMs could support the activity and proliferation of BMSCs but had different effects on their osteogenic differentiation. The 14d-ECM promoted the osteogenesis of BMSCs significantly compared with the other dECMs. Proteomic analysis demonstrated that the composition of dECMs changed over time. The 14d ECM had higher amounts of collagen type IV alpha 2 chain (COL4A2) than the other dECMs. Furthermore, COL4A2 was obviously enriched in the activated focal adhesion kinase (FAK)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways. Thus, the 14d-ECM could promote the osteogenic differentiation of BMSCs, which might be related to the high content of COL4A2 in the 14d-ECM by activating the FAK/PI3K/AKT signaling pathways.
14d-ECM secreted by BMSCs promotes the osteogenic differentiation of BMSCs. The underlying mechanism may be related to COL4A2 in 14d-ECM
via
activation of the FAK/PI3K/AKT signaling pathway.</description><identifier>ISSN: 2050-750X</identifier><identifier>EISSN: 2050-7518</identifier><identifier>DOI: 10.1039/d2tb00721e</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>1-Phosphatidylinositol 3-kinase ; AKT protein ; Bone marrow ; Cell differentiation ; Collagen (type IV) ; Composition ; Differentiation (biology) ; Extracellular matrix ; Focal adhesion kinase ; Kinases ; Mesenchymal stem cells ; Microenvironments ; Osteogenesis ; Phosphatidylinositol 4,5-diphosphate ; Proteomics ; Regulatory mechanisms (biology) ; Signal transduction ; Signaling ; Stem cells</subject><ispartof>Journal of materials chemistry. B, Materials for biology and medicine, 2022-08, Vol.1 (32), p.6171-618</ispartof><rights>Copyright Royal Society of Chemistry 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-7f746d8debff0060b71e7fdc8b534ce7cf9cb22625983b5f5fe1b6c47e0627ec3</citedby><cites>FETCH-LOGICAL-c314t-7f746d8debff0060b71e7fdc8b534ce7cf9cb22625983b5f5fe1b6c47e0627ec3</cites><orcidid>0000-0002-2879-6587</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Xu, Fei</creatorcontrib><creatorcontrib>Zheng, Ziran</creatorcontrib><creatorcontrib>Yao, Mianfeng</creatorcontrib><creatorcontrib>Zhu, Feiya</creatorcontrib><creatorcontrib>Shen, Ting</creatorcontrib><creatorcontrib>Li, Jiang</creatorcontrib><creatorcontrib>Zhu, Chao</creatorcontrib><creatorcontrib>Yang, Tianru</creatorcontrib><creatorcontrib>Shao, Mengying</creatorcontrib><creatorcontrib>Wan, Zicheng</creatorcontrib><creatorcontrib>Fang, Changyun</creatorcontrib><title>A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells</title><title>Journal of materials chemistry. B, Materials for biology and medicine</title><description>A cell-derived decellularized extracellular matrix (dECM) plays a vital role in controlling cell functions because of its similarity to the
in vivo
microenvironment. In the process of stem cell differentiation, the composition of the dECM is not constant but is dynamically remolded. However, there is little information regarding the dynamic regulation by the dECM of the osteogenic differentiation of stem cells. Herein, four types of stepwise dECMs (0, 7, 14, and 21 d-ECM) were prepared from bone marrow-derived mesenchymal stem cells (BMSCs) undergoing osteogenic differentiation for 0, 7, 14, and 21 days after decellularization.
In vitro
experiments were designed to study the regulation of BMSC osteogenesis by dECMs. The results showed that all the dECMs could support the activity and proliferation of BMSCs but had different effects on their osteogenic differentiation. The 14d-ECM promoted the osteogenesis of BMSCs significantly compared with the other dECMs. Proteomic analysis demonstrated that the composition of dECMs changed over time. The 14d ECM had higher amounts of collagen type IV alpha 2 chain (COL4A2) than the other dECMs. Furthermore, COL4A2 was obviously enriched in the activated focal adhesion kinase (FAK)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways. Thus, the 14d-ECM could promote the osteogenic differentiation of BMSCs, which might be related to the high content of COL4A2 in the 14d-ECM by activating the FAK/PI3K/AKT signaling pathways.
14d-ECM secreted by BMSCs promotes the osteogenic differentiation of BMSCs. The underlying mechanism may be related to COL4A2 in 14d-ECM
via
activation of the FAK/PI3K/AKT signaling pathway.</description><subject>1-Phosphatidylinositol 3-kinase</subject><subject>AKT protein</subject><subject>Bone marrow</subject><subject>Cell differentiation</subject><subject>Collagen (type IV)</subject><subject>Composition</subject><subject>Differentiation (biology)</subject><subject>Extracellular matrix</subject><subject>Focal adhesion kinase</subject><subject>Kinases</subject><subject>Mesenchymal stem cells</subject><subject>Microenvironments</subject><subject>Osteogenesis</subject><subject>Phosphatidylinositol 4,5-diphosphate</subject><subject>Proteomics</subject><subject>Regulatory mechanisms (biology)</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Stem cells</subject><issn>2050-750X</issn><issn>2050-7518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNpdkUtLxDAUhYsoKOrGvRBwI0I1jzbpLH0rCG4U3JU0vZmJNsmYZNTxT_kXzTg-wGxySb5z7k1OUewQfEgwGx31NHUYC0pgpdiguMalqEmz-lvjh_ViO8ZHnFdDeMOqjeLjGAUYzwaZfJgjC2oinYkWeY0kigmmryYC8rnyY3AQTSytsUY9GTdGPSgYhiwO5h16BG8pyJ8TZGUK5g15h9Lkz8Eo1ButIYBLRiaTr3OrzjvIghD8a9lDMC_ZzUIEpyZzK4fFIBYtnONWsablEGH7e98s7i_O706vypvby-vT45tSMVKlUmhR8b7podMaY447QUDoXjVdzSoFQumR6ijltB41rKt1rYF0XFUCMKcCFNss9pe-0-CfZxBTa01cTCAd-FlsKW-yPH8jy-jeP_TRz4LL07VUYMY4Z4Rn6mBJqeBjDKDbaTD5yfOW4HYRX3tG706-4jvP8O4SDlH9cn_xsk_q_ZyG</recordid><startdate>20220817</startdate><enddate>20220817</enddate><creator>Xu, Fei</creator><creator>Zheng, Ziran</creator><creator>Yao, Mianfeng</creator><creator>Zhu, Feiya</creator><creator>Shen, Ting</creator><creator>Li, Jiang</creator><creator>Zhu, Chao</creator><creator>Yang, Tianru</creator><creator>Shao, Mengying</creator><creator>Wan, Zicheng</creator><creator>Fang, Changyun</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2879-6587</orcidid></search><sort><creationdate>20220817</creationdate><title>A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells</title><author>Xu, Fei ; 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B, Materials for biology and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Fei</au><au>Zheng, Ziran</au><au>Yao, Mianfeng</au><au>Zhu, Feiya</au><au>Shen, Ting</au><au>Li, Jiang</au><au>Zhu, Chao</au><au>Yang, Tianru</au><au>Shao, Mengying</au><au>Wan, Zicheng</au><au>Fang, Changyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells</atitle><jtitle>Journal of materials chemistry. B, Materials for biology and medicine</jtitle><date>2022-08-17</date><risdate>2022</risdate><volume>1</volume><issue>32</issue><spage>6171</spage><epage>618</epage><pages>6171-618</pages><issn>2050-750X</issn><eissn>2050-7518</eissn><abstract>A cell-derived decellularized extracellular matrix (dECM) plays a vital role in controlling cell functions because of its similarity to the
in vivo
microenvironment. In the process of stem cell differentiation, the composition of the dECM is not constant but is dynamically remolded. However, there is little information regarding the dynamic regulation by the dECM of the osteogenic differentiation of stem cells. Herein, four types of stepwise dECMs (0, 7, 14, and 21 d-ECM) were prepared from bone marrow-derived mesenchymal stem cells (BMSCs) undergoing osteogenic differentiation for 0, 7, 14, and 21 days after decellularization.
In vitro
experiments were designed to study the regulation of BMSC osteogenesis by dECMs. The results showed that all the dECMs could support the activity and proliferation of BMSCs but had different effects on their osteogenic differentiation. The 14d-ECM promoted the osteogenesis of BMSCs significantly compared with the other dECMs. Proteomic analysis demonstrated that the composition of dECMs changed over time. The 14d ECM had higher amounts of collagen type IV alpha 2 chain (COL4A2) than the other dECMs. Furthermore, COL4A2 was obviously enriched in the activated focal adhesion kinase (FAK)/phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways. Thus, the 14d-ECM could promote the osteogenic differentiation of BMSCs, which might be related to the high content of COL4A2 in the 14d-ECM by activating the FAK/PI3K/AKT signaling pathways.
14d-ECM secreted by BMSCs promotes the osteogenic differentiation of BMSCs. The underlying mechanism may be related to COL4A2 in 14d-ECM
via
activation of the FAK/PI3K/AKT signaling pathway.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d2tb00721e</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2879-6587</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of materials chemistry. B, Materials for biology and medicine, 2022-08, Vol.1 (32), p.6171-618 |
| issn | 2050-750X 2050-7518 |
| language | eng |
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| source | Royal Society Of Chemistry Journals 2008- |
| subjects | 1-Phosphatidylinositol 3-kinase AKT protein Bone marrow Cell differentiation Collagen (type IV) Composition Differentiation (biology) Extracellular matrix Focal adhesion kinase Kinases Mesenchymal stem cells Microenvironments Osteogenesis Phosphatidylinositol 4,5-diphosphate Proteomics Regulatory mechanisms (biology) Signal transduction Signaling Stem cells |
| title | A regulatory mechanism of a stepwise osteogenesis-mimicking decellularized extracellular matrix on the osteogenic differentiation of bone marrow-derived mesenchymal stem cells |
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