In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb
Objective: To investigate the in vitro antidiabetic effect on the leaf extracts of Jatropha glandulifera Roxb. Methods: The Preliminary phytochemical screening was investigated on extracts using standard procedure. The in vitro α-amylase and α-glucosidase inhibition assay was carried out at differen...
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| Veröffentlicht in: | Research journal of pharmacy and technology 2022-06, Vol.15 (6), p.2493-2497 |
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| creator | M, Sathish V, Nandhini R, Suresh |
| description | Objective: To investigate the in vitro antidiabetic effect on the leaf extracts of Jatropha glandulifera Roxb. Methods: The Preliminary phytochemical screening was investigated on extracts using standard procedure. The in vitro α-amylase and α-glucosidase inhibition assay was carried out at different concentration such as 10, 20, 40, 80, 160, and 320µg/ml of extracts. The acarbose and voglibose were used as standards respectively. The absorbance was recorded by spectrophotometer. The percentage inhibition and IC50 value were calculated for both the assays. Results: The ethyl acetate extract of Jatropha glandulifera Roxb has shown good inhibition against α-amylase and α-glucosidase with an IC50 value of 21.6137 and 25.8257µg/ml respectively. The phytochemical investigation revealed that the ethyl acetate extract have various phytochemical constituents compared to other extracts like alkaloids, carbohydrates, saponins, Flavonoids and glycosides. These phytoconstitients may be responsible for observed anti diabetic activity. Conclusion: This study concludes that the ethyl acetate extract of Jatropha glandulifera Roxb exhibits better inhibitory activity against both α-amylase and α-glucosidase. |
| doi_str_mv | 10.52711/0974-360X.2022.00416 |
| format | Article |
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Methods: The Preliminary phytochemical screening was investigated on extracts using standard procedure. The in vitro α-amylase and α-glucosidase inhibition assay was carried out at different concentration such as 10, 20, 40, 80, 160, and 320µg/ml of extracts. The acarbose and voglibose were used as standards respectively. The absorbance was recorded by spectrophotometer. The percentage inhibition and IC50 value were calculated for both the assays. Results: The ethyl acetate extract of Jatropha glandulifera Roxb has shown good inhibition against α-amylase and α-glucosidase with an IC50 value of 21.6137 and 25.8257µg/ml respectively. The phytochemical investigation revealed that the ethyl acetate extract have various phytochemical constituents compared to other extracts like alkaloids, carbohydrates, saponins, Flavonoids and glycosides. These phytoconstitients may be responsible for observed anti diabetic activity. Conclusion: This study concludes that the ethyl acetate extract of Jatropha glandulifera Roxb exhibits better inhibitory activity against both α-amylase and α-glucosidase.</description><identifier>ISSN: 0974-3618</identifier><identifier>EISSN: 0974-360X</identifier><identifier>EISSN: 0974-306X</identifier><identifier>DOI: 10.52711/0974-360X.2022.00416</identifier><language>eng</language><publisher>Raipur: A&V Publications</publisher><subject>Antidiabetics ; Ayurvedic medicine ; Cancer ; Carbohydrates ; Diabetes ; Enzymes ; Ethanol ; Flavonoids ; Hyperglycemia ; Insulin ; Investigations ; Kinases ; Oils & fats ; Phytochemicals</subject><ispartof>Research journal of pharmacy and technology, 2022-06, Vol.15 (6), p.2493-2497</ispartof><rights>Copyright A&V Publications Jun 2022</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c144t-5bb76481c4e5c3b288fee6eed6bdd72ab845f94b21419950e2dd3b0ef184746e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>M, Sathish</creatorcontrib><creatorcontrib>V, Nandhini</creatorcontrib><creatorcontrib>R, Suresh</creatorcontrib><title>In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb</title><title>Research journal of pharmacy and technology</title><description>Objective: To investigate the in vitro antidiabetic effect on the leaf extracts of Jatropha glandulifera Roxb. Methods: The Preliminary phytochemical screening was investigated on extracts using standard procedure. The in vitro α-amylase and α-glucosidase inhibition assay was carried out at different concentration such as 10, 20, 40, 80, 160, and 320µg/ml of extracts. The acarbose and voglibose were used as standards respectively. The absorbance was recorded by spectrophotometer. The percentage inhibition and IC50 value were calculated for both the assays. Results: The ethyl acetate extract of Jatropha glandulifera Roxb has shown good inhibition against α-amylase and α-glucosidase with an IC50 value of 21.6137 and 25.8257µg/ml respectively. The phytochemical investigation revealed that the ethyl acetate extract have various phytochemical constituents compared to other extracts like alkaloids, carbohydrates, saponins, Flavonoids and glycosides. These phytoconstitients may be responsible for observed anti diabetic activity. Conclusion: This study concludes that the ethyl acetate extract of Jatropha glandulifera Roxb exhibits better inhibitory activity against both α-amylase and α-glucosidase.</description><subject>Antidiabetics</subject><subject>Ayurvedic medicine</subject><subject>Cancer</subject><subject>Carbohydrates</subject><subject>Diabetes</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>Flavonoids</subject><subject>Hyperglycemia</subject><subject>Insulin</subject><subject>Investigations</subject><subject>Kinases</subject><subject>Oils & fats</subject><subject>Phytochemicals</subject><issn>0974-3618</issn><issn>0974-360X</issn><issn>0974-306X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNo9kF9LwzAUxYMoOHQfQQj43JmkSdo-juGfyUAQBd9C0t64jLaZSSubn952G7sv93I491zuD6E7SmaCZZQ-kCLjSSrJ14wRxmaEcCov0OQsX55nml-jaYwbMpTMBeP5BDXLFv-6Lng8r7drjXWzr3UErNvqpHzXfemjq0YV2r99A9i1a2dc53yLvcU1aIth1wVddnEUXvWQd1wdYvraWQgav_uduUVXVtcRpqd-gz6fHj8WL8nq7Xm5mK-SknLeJcKYTPKclhxEmRqW5xZAAlTSVFXGtMm5sAU3jHJaFIIAq6rUELA05xmXkN6g-2PuNvifHmKnNr4P7XBSMVlkVAzvZ4NLHF1l8DEGsGobXKPDXlGiDnDVSE6NFNUIVx3gpv-cbm31</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>M, Sathish</creator><creator>V, Nandhini</creator><creator>R, Suresh</creator><general>A&V Publications</general><scope>AAYXX</scope><scope>CITATION</scope><scope>04Q</scope><scope>04S</scope><scope>04W</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20220601</creationdate><title>In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb</title><author>M, Sathish ; V, Nandhini ; R, Suresh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c144t-5bb76481c4e5c3b288fee6eed6bdd72ab845f94b21419950e2dd3b0ef184746e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antidiabetics</topic><topic>Ayurvedic medicine</topic><topic>Cancer</topic><topic>Carbohydrates</topic><topic>Diabetes</topic><topic>Enzymes</topic><topic>Ethanol</topic><topic>Flavonoids</topic><topic>Hyperglycemia</topic><topic>Insulin</topic><topic>Investigations</topic><topic>Kinases</topic><topic>Oils & fats</topic><topic>Phytochemicals</topic><toplevel>online_resources</toplevel><creatorcontrib>M, Sathish</creatorcontrib><creatorcontrib>V, Nandhini</creatorcontrib><creatorcontrib>R, Suresh</creatorcontrib><collection>CrossRef</collection><collection>India Database</collection><collection>India Database: Business</collection><collection>India Database: Science & Technology</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Research journal of pharmacy and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>M, Sathish</au><au>V, Nandhini</au><au>R, Suresh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb</atitle><jtitle>Research journal of pharmacy and technology</jtitle><date>2022-06-01</date><risdate>2022</risdate><volume>15</volume><issue>6</issue><spage>2493</spage><epage>2497</epage><pages>2493-2497</pages><issn>0974-3618</issn><eissn>0974-360X</eissn><eissn>0974-306X</eissn><abstract>Objective: To investigate the in vitro antidiabetic effect on the leaf extracts of Jatropha glandulifera Roxb. Methods: The Preliminary phytochemical screening was investigated on extracts using standard procedure. The in vitro α-amylase and α-glucosidase inhibition assay was carried out at different concentration such as 10, 20, 40, 80, 160, and 320µg/ml of extracts. The acarbose and voglibose were used as standards respectively. The absorbance was recorded by spectrophotometer. The percentage inhibition and IC50 value were calculated for both the assays. Results: The ethyl acetate extract of Jatropha glandulifera Roxb has shown good inhibition against α-amylase and α-glucosidase with an IC50 value of 21.6137 and 25.8257µg/ml respectively. The phytochemical investigation revealed that the ethyl acetate extract have various phytochemical constituents compared to other extracts like alkaloids, carbohydrates, saponins, Flavonoids and glycosides. These phytoconstitients may be responsible for observed anti diabetic activity. Conclusion: This study concludes that the ethyl acetate extract of Jatropha glandulifera Roxb exhibits better inhibitory activity against both α-amylase and α-glucosidase.</abstract><cop>Raipur</cop><pub>A&V Publications</pub><doi>10.52711/0974-360X.2022.00416</doi><tpages>5</tpages></addata></record> |
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| subjects | Antidiabetics Ayurvedic medicine Cancer Carbohydrates Diabetes Enzymes Ethanol Flavonoids Hyperglycemia Insulin Investigations Kinases Oils & fats Phytochemicals |
| title | In vitro Alpha amylase and Alpha glucosidase enzyme inhibition of leaf extracts of Jatropha glandulifera Roxb |
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