A Novel Continuous Enzyme Coupled Colorimetric Assay for Phospholipase A2 and its Application in the Determination of Catalytic Activity of Oil-Body–Associated Oleosin Protein

The present work reports a novel, continuous, specific, and colorimetric coupled method for the assay of phospholipase A 2 (PLA 2 ), which involves use of acyl-CoA synthetase (ACS) as coupling enzyme and Dilinoleoyl Phosphatidylcholine (DLPC) as substrate. This method is based on the principle that...

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Veröffentlicht in:	Food analytical methods 2022-08, Vol.15 (8), p.2155-2162
Hauptverfasser:	Kumari, Sweta, Gupta, Om Prakash, Kumar, Sandeep, Sasi, Minnu, Arpitha, S. R, Amirtham, D., Mishra, Chandra Bhushan, Thimmegowda, Vinutha, Krishnan, Veda, Sachdev, Archana, Kumar, Rajeev Ranjan, Dahuja, Anil
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical Chemistry Assaying Catalytic activity Chemistry Chemistry and Materials Science Chemistry/Food Science Coenzyme A Colorimetry Fatty acids Food Science Lecithin Microbiology Oleosin Phosphatidylcholine Phospholipase Phospholipase A2 Substrates
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