Rapid and low-cost diagnosis of Japanese yam mosaic virus infection in Chinese yam (Dioscorea polystachya) leaves by a print-capture RT-PCR
A rapid diagnostic method for Japanese yam mosaic virus (JYMV) infection in Chinese yam ( Dioscorea polystachya ) by a print-capture RT-PCR was developed. The cut surface of a rolled leaf of a JYMV-infected Chinese yam was stamped onto a nitrocellulose membrane. The clipped membrane was boiled in el...
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| Veröffentlicht in: | Journal of general plant pathology : JGPP 2017-07, Vol.83 (4), p.189-196 |
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| creator | Mochizuki, Tomofumi Iwamoto, Itsuki Atarashi, Aya Ohki, Satoshi T. Kikukawa, Hiroyuki |
| description | A rapid diagnostic method for
Japanese yam mosaic virus
(JYMV) infection in Chinese yam (
Dioscorea polystachya
) by a print-capture RT-PCR was developed. The cut surface of a rolled leaf of a JYMV-infected Chinese yam was stamped onto a nitrocellulose membrane. The clipped membrane was boiled in eluting solution to elute the RNA for use as the template in RT-nested PCR. Although JYMV RNA was detectable in most infected plants in the first PCR of the print-capture RT-PCR, it was detected from all samples in the nested PCR. The tissue-printed membranes could be preserved for at least 3 months at 4 °C for JYMV detection. |
| doi_str_mv | 10.1007/s10327-017-0719-6 |
| format | Article |
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Japanese yam mosaic virus
(JYMV) infection in Chinese yam (
Dioscorea polystachya
) by a print-capture RT-PCR was developed. The cut surface of a rolled leaf of a JYMV-infected Chinese yam was stamped onto a nitrocellulose membrane. The clipped membrane was boiled in eluting solution to elute the RNA for use as the template in RT-nested PCR. Although JYMV RNA was detectable in most infected plants in the first PCR of the print-capture RT-PCR, it was detected from all samples in the nested PCR. The tissue-printed membranes could be preserved for at least 3 months at 4 °C for JYMV detection.</description><identifier>ISSN: 1345-2630</identifier><identifier>EISSN: 1610-739X</identifier><identifier>DOI: 10.1007/s10327-017-0719-6</identifier><language>eng</language><publisher>Tokyo: Springer Japan</publisher><subject>Agriculture ; Biomedical and Life Sciences ; Cellulose esters ; Cellulose nitrate ; Diagnosis ; Diagnostic systems ; Dioscorea japonica ; Dioscorea polystachya ; Leaves ; Life Sciences ; Membranes ; Microbiology ; Pathogens ; Plant diseases ; Plant Pathology ; Plant virus diseases ; Polymerase chain reaction ; Ribonucleic acid ; RNA ; Technique ; Vegetables ; Viruses</subject><ispartof>Journal of general plant pathology : JGPP, 2017-07, Vol.83 (4), p.189-196</ispartof><rights>The Phytopathological Society of Japan and Springer Japan 2017</rights><rights>Journal of General Plant Pathology is a copyright of Springer, 2017.</rights><rights>The Phytopathological Society of Japan and Springer Japan 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c344t-d555483d61b7a05b5fa073c2790b610a66b8f738c9fc0baea0baba6b3a0d7a773</citedby><cites>FETCH-LOGICAL-c344t-d555483d61b7a05b5fa073c2790b610a66b8f738c9fc0baea0baba6b3a0d7a773</cites><orcidid>0000-0002-9129-4555</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10327-017-0719-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10327-017-0719-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids></links><search><creatorcontrib>Mochizuki, Tomofumi</creatorcontrib><creatorcontrib>Iwamoto, Itsuki</creatorcontrib><creatorcontrib>Atarashi, Aya</creatorcontrib><creatorcontrib>Ohki, Satoshi T.</creatorcontrib><creatorcontrib>Kikukawa, Hiroyuki</creatorcontrib><title>Rapid and low-cost diagnosis of Japanese yam mosaic virus infection in Chinese yam (Dioscorea polystachya) leaves by a print-capture RT-PCR</title><title>Journal of general plant pathology : JGPP</title><addtitle>J Gen Plant Pathol</addtitle><description>A rapid diagnostic method for
Japanese yam mosaic virus
(JYMV) infection in Chinese yam (
Dioscorea polystachya
) by a print-capture RT-PCR was developed. The cut surface of a rolled leaf of a JYMV-infected Chinese yam was stamped onto a nitrocellulose membrane. The clipped membrane was boiled in eluting solution to elute the RNA for use as the template in RT-nested PCR. Although JYMV RNA was detectable in most infected plants in the first PCR of the print-capture RT-PCR, it was detected from all samples in the nested PCR. The tissue-printed membranes could be preserved for at least 3 months at 4 °C for JYMV detection.</description><subject>Agriculture</subject><subject>Biomedical and Life Sciences</subject><subject>Cellulose esters</subject><subject>Cellulose nitrate</subject><subject>Diagnosis</subject><subject>Diagnostic systems</subject><subject>Dioscorea japonica</subject><subject>Dioscorea polystachya</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Membranes</subject><subject>Microbiology</subject><subject>Pathogens</subject><subject>Plant diseases</subject><subject>Plant Pathology</subject><subject>Plant virus diseases</subject><subject>Polymerase chain reaction</subject><subject>Ribonucleic 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and low-cost diagnosis of Japanese yam mosaic virus infection in Chinese yam (Dioscorea polystachya) leaves by a print-capture RT-PCR</title><author>Mochizuki, Tomofumi ; Iwamoto, Itsuki ; Atarashi, Aya ; Ohki, Satoshi T. ; Kikukawa, Hiroyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c344t-d555483d61b7a05b5fa073c2790b610a66b8f738c9fc0baea0baba6b3a0d7a773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Agriculture</topic><topic>Biomedical and Life Sciences</topic><topic>Cellulose esters</topic><topic>Cellulose nitrate</topic><topic>Diagnosis</topic><topic>Diagnostic systems</topic><topic>Dioscorea japonica</topic><topic>Dioscorea polystachya</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Membranes</topic><topic>Microbiology</topic><topic>Pathogens</topic><topic>Plant diseases</topic><topic>Plant Pathology</topic><topic>Plant virus 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pathology : JGPP</jtitle><stitle>J Gen Plant Pathol</stitle><date>2017-07-01</date><risdate>2017</risdate><volume>83</volume><issue>4</issue><spage>189</spage><epage>196</epage><pages>189-196</pages><issn>1345-2630</issn><eissn>1610-739X</eissn><abstract>A rapid diagnostic method for
Japanese yam mosaic virus
(JYMV) infection in Chinese yam (
Dioscorea polystachya
) by a print-capture RT-PCR was developed. The cut surface of a rolled leaf of a JYMV-infected Chinese yam was stamped onto a nitrocellulose membrane. The clipped membrane was boiled in eluting solution to elute the RNA for use as the template in RT-nested PCR. Although JYMV RNA was detectable in most infected plants in the first PCR of the print-capture RT-PCR, it was detected from all samples in the nested PCR. The tissue-printed membranes could be preserved for at least 3 months at 4 °C for JYMV detection.</abstract><cop>Tokyo</cop><pub>Springer Japan</pub><doi>10.1007/s10327-017-0719-6</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-9129-4555</orcidid></addata></record> |
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| ispartof | Journal of general plant pathology : JGPP, 2017-07, Vol.83 (4), p.189-196 |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Agriculture Biomedical and Life Sciences Cellulose esters Cellulose nitrate Diagnosis Diagnostic systems Dioscorea japonica Dioscorea polystachya Leaves Life Sciences Membranes Microbiology Pathogens Plant diseases Plant Pathology Plant virus diseases Polymerase chain reaction Ribonucleic acid RNA Technique Vegetables Viruses |
| title | Rapid and low-cost diagnosis of Japanese yam mosaic virus infection in Chinese yam (Dioscorea polystachya) leaves by a print-capture RT-PCR |
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