Genetic Polymorphism of BoLA-DRB3.2 Locus in Ukrainian Cattle Breeds
The results of the study of BoLA-DRB3 gene polymorphism in four domestic breeds are presented: Ukrainian black-and-white dairy ( n = 293), Ukrainian red-and-white dairy ( n = 131), Gray Ukrainian ( n = 93), and Ukrainian white-headed ( n = 49). The allele frequencies were determined based on the ana...
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| description | The results of the study of BoLA-DRB3 gene polymorphism in four domestic breeds are presented: Ukrainian black-and-white dairy (
n
= 293), Ukrainian red-and-white dairy (
n
= 131), Gray Ukrainian (
n
= 93), and Ukrainian white-headed (
n
= 49). The allele frequencies were determined based on the analysis of restriction fragment length polymorphism of the products of amplification of BoLA-DRB3 gene exon 2. Genetic profiles of the breeds were formed according to the following indices: allele (
P
a
) and genotype (
G
a
) frequencies, observed (
H
o
) and expected (
H
e
) heterozygosity, Wright fixation index (
F
IS
), total (
N
a
) and effective (
A
e
) number of alleles, and Shannon (
I
) and Pielou (
J
) efficiency indices. The maximal number of alleles were in the experimental sample of Ukrainian black-and-white dairy breed (
N
a
= 37). In Ukrainian red-and-white dairy, Ukrainian white-headed, and Gray Ukrainian breeds, 31, 29, and 28 variants were found, respectively. In two breeds, the alleles with “no established nomenclature” were typed: Gray Ukrainian, jba, *jab, *jbb, *nad and *nda; Ukrainian white-headed, *nab, *mdb, *iab, *gbb, *fbd, *naa; their portion was 8.9 and 7.1%, respectively. The portion of “informative” alleles was: Ukrainian black-and-white dairy, 55.8% (seven alleles); Ukrainian red-and-white dairy, 60.4% (six); Gray Ukrainian, 69.9% (four); Ukrainian white-headed, 65.3% (seven). The expected heterozygosity and Wright index had the following values: Ukrainian black-and-white dairy,
H
e
= 0.942,
F
IS
= 0.022; Ukrainian red-and-white dairy,
H
e
= 0.93,
F
IS
= 0.032; Gray Ukrainian,
H
e
= 0.774,
F
IS
= 0.041; Ukrainian white-headed,
H
e
= 0.927,
F
IS
= –0.035. According to biodiversity indices, the greatest variability is typical for the Ukrainian black-and-white dairy breed (
A
e
= 17.2;
I
= 3.13;
J
= 0.6). The Ukrainian white-headed (
A
e
= 13.7;
I
= 2.93;
J
= 0.61) and Ukrainian red-and-white dairy (
A
e
= 11.5;
I
= 2.87;
J
= 0.558) cattle also had a high level of variability. A low level of biodiversity was found in Gray Ukrainian breed (
A
e
= 4.42;
I
= 2.21;
J
= 0.455). The analysis of BoLA-DRB3 gene exon 2 polymorphism demonstrated that the greatest genetic diversity is typical for the Ukrainian black-and-while dairy breed. According to genetic distances, the domestic breeds are combined in a single cluster with Holsteins, which indicates their genetic relationship. |
| doi_str_mv | 10.3103/S0095452722040089 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2692733286</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2692733286</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-772e13674b538dda505f44b117ab9779e1218d6774f2c5960bb520e58be4ac383</originalsourceid><addsrcrecordid>eNp1kMtOwzAQRS0EEqXwAewssU4Ze_yIl31AQYoEArqOnMSBlDYudrLo35OqSCwQq1ncc-5Il5BrBhNkgLevAEYKyTXnIABSc0JGzKBIjBBwSkaHODnk5-QixjWAVBxxRBZL17quKemz3-y3Puw-mrilvqYzn02TxcsMJ5xmvuwjbVq6-gy2aRvb0rntuo2js-BcFS_JWW030V393DFZ3d-9zR-S7Gn5OJ9mSYlMdYnW3DFUWhQS06qyEmQtRMGYtoXR2jjGWVoprUXNS2kUFIXk4GRaOGFLTHFMbo69u-C_ehe7fO370A4vc64M14g8VQPFjlQZfIzB1fkuNFsb9jmD_DBW_mesweFHJw5s--7Cb_P_0jfkuWf9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2692733286</pqid></control><display><type>article</type><title>Genetic Polymorphism of BoLA-DRB3.2 Locus in Ukrainian Cattle Breeds</title><source>SpringerNature Journals</source><creator>Suprovych, T. M. ; Salyha, Yu. T. ; Suprovych, M. P. ; Fedorovych, E. I. ; Fedorovych, V. V. ; Chornyj, I. O.</creator><creatorcontrib>Suprovych, T. M. ; Salyha, Yu. T. ; Suprovych, M. P. ; Fedorovych, E. I. ; Fedorovych, V. V. ; Chornyj, I. O.</creatorcontrib><description>The results of the study of BoLA-DRB3 gene polymorphism in four domestic breeds are presented: Ukrainian black-and-white dairy (
n
= 293), Ukrainian red-and-white dairy (
n
= 131), Gray Ukrainian (
n
= 93), and Ukrainian white-headed (
n
= 49). The allele frequencies were determined based on the analysis of restriction fragment length polymorphism of the products of amplification of BoLA-DRB3 gene exon 2. Genetic profiles of the breeds were formed according to the following indices: allele (
P
a
) and genotype (
G
a
) frequencies, observed (
H
o
) and expected (
H
e
) heterozygosity, Wright fixation index (
F
IS
), total (
N
a
) and effective (
A
e
) number of alleles, and Shannon (
I
) and Pielou (
J
) efficiency indices. The maximal number of alleles were in the experimental sample of Ukrainian black-and-white dairy breed (
N
a
= 37). In Ukrainian red-and-white dairy, Ukrainian white-headed, and Gray Ukrainian breeds, 31, 29, and 28 variants were found, respectively. In two breeds, the alleles with “no established nomenclature” were typed: Gray Ukrainian, jba, *jab, *jbb, *nad and *nda; Ukrainian white-headed, *nab, *mdb, *iab, *gbb, *fbd, *naa; their portion was 8.9 and 7.1%, respectively. The portion of “informative” alleles was: Ukrainian black-and-white dairy, 55.8% (seven alleles); Ukrainian red-and-white dairy, 60.4% (six); Gray Ukrainian, 69.9% (four); Ukrainian white-headed, 65.3% (seven). The expected heterozygosity and Wright index had the following values: Ukrainian black-and-white dairy,
H
e
= 0.942,
F
IS
= 0.022; Ukrainian red-and-white dairy,
H
e
= 0.93,
F
IS
= 0.032; Gray Ukrainian,
H
e
= 0.774,
F
IS
= 0.041; Ukrainian white-headed,
H
e
= 0.927,
F
IS
= –0.035. According to biodiversity indices, the greatest variability is typical for the Ukrainian black-and-white dairy breed (
A
e
= 17.2;
I
= 3.13;
J
= 0.6). The Ukrainian white-headed (
A
e
= 13.7;
I
= 2.93;
J
= 0.61) and Ukrainian red-and-white dairy (
A
e
= 11.5;
I
= 2.87;
J
= 0.558) cattle also had a high level of variability. A low level of biodiversity was found in Gray Ukrainian breed (
A
e
= 4.42;
I
= 2.21;
J
= 0.455). The analysis of BoLA-DRB3 gene exon 2 polymorphism demonstrated that the greatest genetic diversity is typical for the Ukrainian black-and-while dairy breed. According to genetic distances, the domestic breeds are combined in a single cluster with Holsteins, which indicates their genetic relationship.</description><identifier>ISSN: 0095-4527</identifier><identifier>EISSN: 1934-9440</identifier><identifier>DOI: 10.3103/S0095452722040089</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Alleles ; Biodiversity ; Biomedical and Life Sciences ; Biomedicine ; Gene frequency ; Gene polymorphism ; Genetic distance ; Genetic diversity ; Genetic relationship ; Heterozygosity ; Human Genetics ; Nomenclature ; Polymorphism ; Restriction fragment length polymorphism</subject><ispartof>Cytology and genetics, 2022-08, Vol.56 (4), p.319-330</ispartof><rights>Allerton Press, Inc. 2022. ISSN 0095-4527, Cytology and Genetics, 2022, Vol. 56, No. 4, pp. 319–330. © Allerton Press, Inc., 2022. Ukrainian Text © The Author(s), 2022, published in Tsitologiya i Genetika, 2022, Vol. 56, No. 4, pp. 10–23.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-772e13674b538dda505f44b117ab9779e1218d6774f2c5960bb520e58be4ac383</citedby><cites>FETCH-LOGICAL-c316t-772e13674b538dda505f44b117ab9779e1218d6774f2c5960bb520e58be4ac383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.3103/S0095452722040089$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.3103/S0095452722040089$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Suprovych, T. M.</creatorcontrib><creatorcontrib>Salyha, Yu. T.</creatorcontrib><creatorcontrib>Suprovych, M. P.</creatorcontrib><creatorcontrib>Fedorovych, E. I.</creatorcontrib><creatorcontrib>Fedorovych, V. V.</creatorcontrib><creatorcontrib>Chornyj, I. O.</creatorcontrib><title>Genetic Polymorphism of BoLA-DRB3.2 Locus in Ukrainian Cattle Breeds</title><title>Cytology and genetics</title><addtitle>Cytol. Genet</addtitle><description>The results of the study of BoLA-DRB3 gene polymorphism in four domestic breeds are presented: Ukrainian black-and-white dairy (
n
= 293), Ukrainian red-and-white dairy (
n
= 131), Gray Ukrainian (
n
= 93), and Ukrainian white-headed (
n
= 49). The allele frequencies were determined based on the analysis of restriction fragment length polymorphism of the products of amplification of BoLA-DRB3 gene exon 2. Genetic profiles of the breeds were formed according to the following indices: allele (
P
a
) and genotype (
G
a
) frequencies, observed (
H
o
) and expected (
H
e
) heterozygosity, Wright fixation index (
F
IS
), total (
N
a
) and effective (
A
e
) number of alleles, and Shannon (
I
) and Pielou (
J
) efficiency indices. The maximal number of alleles were in the experimental sample of Ukrainian black-and-white dairy breed (
N
a
= 37). In Ukrainian red-and-white dairy, Ukrainian white-headed, and Gray Ukrainian breeds, 31, 29, and 28 variants were found, respectively. In two breeds, the alleles with “no established nomenclature” were typed: Gray Ukrainian, jba, *jab, *jbb, *nad and *nda; Ukrainian white-headed, *nab, *mdb, *iab, *gbb, *fbd, *naa; their portion was 8.9 and 7.1%, respectively. The portion of “informative” alleles was: Ukrainian black-and-white dairy, 55.8% (seven alleles); Ukrainian red-and-white dairy, 60.4% (six); Gray Ukrainian, 69.9% (four); Ukrainian white-headed, 65.3% (seven). The expected heterozygosity and Wright index had the following values: Ukrainian black-and-white dairy,
H
e
= 0.942,
F
IS
= 0.022; Ukrainian red-and-white dairy,
H
e
= 0.93,
F
IS
= 0.032; Gray Ukrainian,
H
e
= 0.774,
F
IS
= 0.041; Ukrainian white-headed,
H
e
= 0.927,
F
IS
= –0.035. According to biodiversity indices, the greatest variability is typical for the Ukrainian black-and-white dairy breed (
A
e
= 17.2;
I
= 3.13;
J
= 0.6). The Ukrainian white-headed (
A
e
= 13.7;
I
= 2.93;
J
= 0.61) and Ukrainian red-and-white dairy (
A
e
= 11.5;
I
= 2.87;
J
= 0.558) cattle also had a high level of variability. A low level of biodiversity was found in Gray Ukrainian breed (
A
e
= 4.42;
I
= 2.21;
J
= 0.455). The analysis of BoLA-DRB3 gene exon 2 polymorphism demonstrated that the greatest genetic diversity is typical for the Ukrainian black-and-while dairy breed. According to genetic distances, the domestic breeds are combined in a single cluster with Holsteins, which indicates their genetic relationship.</description><subject>Alleles</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Gene frequency</subject><subject>Gene polymorphism</subject><subject>Genetic distance</subject><subject>Genetic diversity</subject><subject>Genetic relationship</subject><subject>Heterozygosity</subject><subject>Human Genetics</subject><subject>Nomenclature</subject><subject>Polymorphism</subject><subject>Restriction fragment length polymorphism</subject><issn>0095-4527</issn><issn>1934-9440</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOwzAQRS0EEqXwAewssU4Ze_yIl31AQYoEArqOnMSBlDYudrLo35OqSCwQq1ncc-5Il5BrBhNkgLevAEYKyTXnIABSc0JGzKBIjBBwSkaHODnk5-QixjWAVBxxRBZL17quKemz3-y3Puw-mrilvqYzn02TxcsMJ5xmvuwjbVq6-gy2aRvb0rntuo2js-BcFS_JWW030V393DFZ3d-9zR-S7Gn5OJ9mSYlMdYnW3DFUWhQS06qyEmQtRMGYtoXR2jjGWVoprUXNS2kUFIXk4GRaOGFLTHFMbo69u-C_ehe7fO370A4vc64M14g8VQPFjlQZfIzB1fkuNFsb9jmD_DBW_mesweFHJw5s--7Cb_P_0jfkuWf9</recordid><startdate>20220801</startdate><enddate>20220801</enddate><creator>Suprovych, T. M.</creator><creator>Salyha, Yu. T.</creator><creator>Suprovych, M. P.</creator><creator>Fedorovych, E. I.</creator><creator>Fedorovych, V. V.</creator><creator>Chornyj, I. O.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20220801</creationdate><title>Genetic Polymorphism of BoLA-DRB3.2 Locus in Ukrainian Cattle Breeds</title><author>Suprovych, T. M. ; Salyha, Yu. T. ; Suprovych, M. P. ; Fedorovych, E. I. ; Fedorovych, V. V. ; Chornyj, I. O.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-772e13674b538dda505f44b117ab9779e1218d6774f2c5960bb520e58be4ac383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Alleles</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Gene frequency</topic><topic>Gene polymorphism</topic><topic>Genetic distance</topic><topic>Genetic diversity</topic><topic>Genetic relationship</topic><topic>Heterozygosity</topic><topic>Human Genetics</topic><topic>Nomenclature</topic><topic>Polymorphism</topic><topic>Restriction fragment length polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suprovych, T. M.</creatorcontrib><creatorcontrib>Salyha, Yu. T.</creatorcontrib><creatorcontrib>Suprovych, M. P.</creatorcontrib><creatorcontrib>Fedorovych, E. I.</creatorcontrib><creatorcontrib>Fedorovych, V. V.</creatorcontrib><creatorcontrib>Chornyj, I. O.</creatorcontrib><collection>CrossRef</collection><jtitle>Cytology and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suprovych, T. M.</au><au>Salyha, Yu. T.</au><au>Suprovych, M. P.</au><au>Fedorovych, E. I.</au><au>Fedorovych, V. V.</au><au>Chornyj, I. O.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Polymorphism of BoLA-DRB3.2 Locus in Ukrainian Cattle Breeds</atitle><jtitle>Cytology and genetics</jtitle><stitle>Cytol. Genet</stitle><date>2022-08-01</date><risdate>2022</risdate><volume>56</volume><issue>4</issue><spage>319</spage><epage>330</epage><pages>319-330</pages><issn>0095-4527</issn><eissn>1934-9440</eissn><abstract>The results of the study of BoLA-DRB3 gene polymorphism in four domestic breeds are presented: Ukrainian black-and-white dairy (
n
= 293), Ukrainian red-and-white dairy (
n
= 131), Gray Ukrainian (
n
= 93), and Ukrainian white-headed (
n
= 49). The allele frequencies were determined based on the analysis of restriction fragment length polymorphism of the products of amplification of BoLA-DRB3 gene exon 2. Genetic profiles of the breeds were formed according to the following indices: allele (
P
a
) and genotype (
G
a
) frequencies, observed (
H
o
) and expected (
H
e
) heterozygosity, Wright fixation index (
F
IS
), total (
N
a
) and effective (
A
e
) number of alleles, and Shannon (
I
) and Pielou (
J
) efficiency indices. The maximal number of alleles were in the experimental sample of Ukrainian black-and-white dairy breed (
N
a
= 37). In Ukrainian red-and-white dairy, Ukrainian white-headed, and Gray Ukrainian breeds, 31, 29, and 28 variants were found, respectively. In two breeds, the alleles with “no established nomenclature” were typed: Gray Ukrainian, jba, *jab, *jbb, *nad and *nda; Ukrainian white-headed, *nab, *mdb, *iab, *gbb, *fbd, *naa; their portion was 8.9 and 7.1%, respectively. The portion of “informative” alleles was: Ukrainian black-and-white dairy, 55.8% (seven alleles); Ukrainian red-and-white dairy, 60.4% (six); Gray Ukrainian, 69.9% (four); Ukrainian white-headed, 65.3% (seven). The expected heterozygosity and Wright index had the following values: Ukrainian black-and-white dairy,
H
e
= 0.942,
F
IS
= 0.022; Ukrainian red-and-white dairy,
H
e
= 0.93,
F
IS
= 0.032; Gray Ukrainian,
H
e
= 0.774,
F
IS
= 0.041; Ukrainian white-headed,
H
e
= 0.927,
F
IS
= –0.035. According to biodiversity indices, the greatest variability is typical for the Ukrainian black-and-white dairy breed (
A
e
= 17.2;
I
= 3.13;
J
= 0.6). The Ukrainian white-headed (
A
e
= 13.7;
I
= 2.93;
J
= 0.61) and Ukrainian red-and-white dairy (
A
e
= 11.5;
I
= 2.87;
J
= 0.558) cattle also had a high level of variability. A low level of biodiversity was found in Gray Ukrainian breed (
A
e
= 4.42;
I
= 2.21;
J
= 0.455). The analysis of BoLA-DRB3 gene exon 2 polymorphism demonstrated that the greatest genetic diversity is typical for the Ukrainian black-and-while dairy breed. According to genetic distances, the domestic breeds are combined in a single cluster with Holsteins, which indicates their genetic relationship.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.3103/S0095452722040089</doi><tpages>12</tpages></addata></record> |
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| source | SpringerNature Journals |
| subjects | Alleles Biodiversity Biomedical and Life Sciences Biomedicine Gene frequency Gene polymorphism Genetic distance Genetic diversity Genetic relationship Heterozygosity Human Genetics Nomenclature Polymorphism Restriction fragment length polymorphism |
| title | Genetic Polymorphism of BoLA-DRB3.2 Locus in Ukrainian Cattle Breeds |
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