Improved induction of meiotic gynogenesis in Coruh trout, Salmo coruhensis
In this study, water and spermatozoa parameters were evaluated during the reproduction season, as well as the optimal conditions for the retention of the second polar body in Salmo coruhensis eggs obtained with heat‐shock timing, intensity, duration and UV irradiation for gynogenetic production. Spe...
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| Veröffentlicht in: | Aquaculture research 2022-08, Vol.53 (12), p.4327-4337 |
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| creator | Özdemir, Rahmi Can Ekici, Aygül |
| description | In this study, water and spermatozoa parameters were evaluated during the reproduction season, as well as the optimal conditions for the retention of the second polar body in Salmo coruhensis eggs obtained with heat‐shock timing, intensity, duration and UV irradiation for gynogenetic production. Sperm was diluted 1:20 with lightly modified Hank's Balanced Salt Solution, and sperm manifesting 60% motility was obtained with a UV dose of 3600 kJ/cm2. The highest ratios of eyed‐stage, hatching and larval survival were 89.59 ± 2.18%, 82.86 ± 3.11% and 79.61 ± 1.25% in the experimental group exposed to 28.5 °C heat‐shock respectively. As the shocking temperature increased, the rates of eyed‐stage, hatching and larval survival decreased (p |
| doi_str_mv | 10.1111/are.15930 |
| format | Article |
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Sperm was diluted 1:20 with lightly modified Hank's Balanced Salt Solution, and sperm manifesting 60% motility was obtained with a UV dose of 3600 kJ/cm2. The highest ratios of eyed‐stage, hatching and larval survival were 89.59 ± 2.18%, 82.86 ± 3.11% and 79.61 ± 1.25% in the experimental group exposed to 28.5 °C heat‐shock respectively. As the shocking temperature increased, the rates of eyed‐stage, hatching and larval survival decreased (p < 0.05). After 3–7 days of fertilization, all haploid individuals died in all experimental groups. As a result of the morphological examination, no difference was found between the gynogen and control groups at larval and juvenile stages. In karyotype analysis, chromosome numbers of control (C), diploid gynogen (G) and triploid (T) group were 2n = 80 and 3n ≈ 120 respectively. Triploid groups showed 1.5 times higher mean erythrocyte diameter and relative DNA content than G and C groups. Moreover, it was determined that sdY, the salmonid male sex‐determining gene, was not found in all groups that were assumed to be gynogens, and all of them were female (100%). These results indicated that all individuals in the gynogen groups were gynogenetic. The protocol developed in this study can contribute to the production of gynogenetic Coruh trout, the production of mono‐sex species, as well as the genetic performance enhancement studies.</description><identifier>ISSN: 1355-557X</identifier><identifier>EISSN: 1365-2109</identifier><identifier>DOI: 10.1111/are.15930</identifier><language>eng</language><publisher>Oxford: Hindawi Limited</publisher><subject>Biological fertilization ; Chromosomes ; Coruh trout ; Deoxyribonucleic acid ; Diploids ; Diploidy ; DNA ; erythrocyte ; Erythrocytes ; Fertilization ; flow cytometry ; Freshwater fishes ; Gynogenesis ; Haploidy ; Hatching ; Irradiation ; Juveniles ; Karyotypes ; Larvae ; Meiosis ; Saline solutions ; Salmo coruhensis ; sdY gene ; Sex ; Sperm ; Spermatozoa ; Survival ; Trout ; Ultraviolet radiation</subject><ispartof>Aquaculture research, 2022-08, Vol.53 (12), p.4327-4337</ispartof><rights>2022 John Wiley & Sons Ltd.</rights><rights>Copyright © 2022 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2920-b9a1ce4ebaf5495f3060c637ae77e47c19fd3434794d814566d1f6bce7e421a83</cites><orcidid>0000-0003-1285-0304 ; 0000-0001-9986-0868</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fare.15930$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fare.15930$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids></links><search><creatorcontrib>Özdemir, Rahmi Can</creatorcontrib><creatorcontrib>Ekici, Aygül</creatorcontrib><title>Improved induction of meiotic gynogenesis in Coruh trout, Salmo coruhensis</title><title>Aquaculture research</title><description>In this study, water and spermatozoa parameters were evaluated during the reproduction season, as well as the optimal conditions for the retention of the second polar body in Salmo coruhensis eggs obtained with heat‐shock timing, intensity, duration and UV irradiation for gynogenetic production. Sperm was diluted 1:20 with lightly modified Hank's Balanced Salt Solution, and sperm manifesting 60% motility was obtained with a UV dose of 3600 kJ/cm2. The highest ratios of eyed‐stage, hatching and larval survival were 89.59 ± 2.18%, 82.86 ± 3.11% and 79.61 ± 1.25% in the experimental group exposed to 28.5 °C heat‐shock respectively. As the shocking temperature increased, the rates of eyed‐stage, hatching and larval survival decreased (p < 0.05). After 3–7 days of fertilization, all haploid individuals died in all experimental groups. As a result of the morphological examination, no difference was found between the gynogen and control groups at larval and juvenile stages. In karyotype analysis, chromosome numbers of control (C), diploid gynogen (G) and triploid (T) group were 2n = 80 and 3n ≈ 120 respectively. Triploid groups showed 1.5 times higher mean erythrocyte diameter and relative DNA content than G and C groups. Moreover, it was determined that sdY, the salmonid male sex‐determining gene, was not found in all groups that were assumed to be gynogens, and all of them were female (100%). These results indicated that all individuals in the gynogen groups were gynogenetic. The protocol developed in this study can contribute to the production of gynogenetic Coruh trout, the production of mono‐sex species, as well as the genetic performance enhancement studies.</description><subject>Biological fertilization</subject><subject>Chromosomes</subject><subject>Coruh trout</subject><subject>Deoxyribonucleic acid</subject><subject>Diploids</subject><subject>Diploidy</subject><subject>DNA</subject><subject>erythrocyte</subject><subject>Erythrocytes</subject><subject>Fertilization</subject><subject>flow cytometry</subject><subject>Freshwater fishes</subject><subject>Gynogenesis</subject><subject>Haploidy</subject><subject>Hatching</subject><subject>Irradiation</subject><subject>Juveniles</subject><subject>Karyotypes</subject><subject>Larvae</subject><subject>Meiosis</subject><subject>Saline solutions</subject><subject>Salmo coruhensis</subject><subject>sdY gene</subject><subject>Sex</subject><subject>Sperm</subject><subject>Spermatozoa</subject><subject>Survival</subject><subject>Trout</subject><subject>Ultraviolet radiation</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LAzEQhoMoWKsH_0HAk-C2yeZjm2MprVYKgh_gLaTZSd3S3dRkV-m_N3W9OpcZZp53ZngRuqZkRFOMTYARFYqREzSgTIosp0SdHmshMiGK93N0EeOWEMoJowP0uKz3wX9Biaum7Gxb-QZ7h2uofFtZvDk0fgMNxComAM986D5wG3zX3uEXs6s9tscWNAm4RGfO7CJc_eUhelvMX2cP2erpfjmbrjKbq5xka2WoBQ5r4wRXwjEiiZWsMFAUwAtLlSsZZ7xQvJxQLqQsqZNrC2maUzNhQ3TT702Pf3YQW731XWjSSZ1LRRRTSZyo256ywccYwOl9qGoTDpoSfbRKJ6v0r1WJHffsd7WDw_-gnj7Pe8UPztdqfQ</recordid><startdate>202208</startdate><enddate>202208</enddate><creator>Özdemir, Rahmi Can</creator><creator>Ekici, Aygül</creator><general>Hindawi Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-1285-0304</orcidid><orcidid>https://orcid.org/0000-0001-9986-0868</orcidid></search><sort><creationdate>202208</creationdate><title>Improved induction of meiotic gynogenesis in Coruh trout, Salmo coruhensis</title><author>Özdemir, Rahmi Can ; Ekici, Aygül</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2920-b9a1ce4ebaf5495f3060c637ae77e47c19fd3434794d814566d1f6bce7e421a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Biological fertilization</topic><topic>Chromosomes</topic><topic>Coruh trout</topic><topic>Deoxyribonucleic acid</topic><topic>Diploids</topic><topic>Diploidy</topic><topic>DNA</topic><topic>erythrocyte</topic><topic>Erythrocytes</topic><topic>Fertilization</topic><topic>flow cytometry</topic><topic>Freshwater fishes</topic><topic>Gynogenesis</topic><topic>Haploidy</topic><topic>Hatching</topic><topic>Irradiation</topic><topic>Juveniles</topic><topic>Karyotypes</topic><topic>Larvae</topic><topic>Meiosis</topic><topic>Saline solutions</topic><topic>Salmo coruhensis</topic><topic>sdY gene</topic><topic>Sex</topic><topic>Sperm</topic><topic>Spermatozoa</topic><topic>Survival</topic><topic>Trout</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Özdemir, Rahmi Can</creatorcontrib><creatorcontrib>Ekici, Aygül</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Aquaculture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Özdemir, Rahmi Can</au><au>Ekici, Aygül</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved induction of meiotic gynogenesis in Coruh trout, Salmo coruhensis</atitle><jtitle>Aquaculture research</jtitle><date>2022-08</date><risdate>2022</risdate><volume>53</volume><issue>12</issue><spage>4327</spage><epage>4337</epage><pages>4327-4337</pages><issn>1355-557X</issn><eissn>1365-2109</eissn><abstract>In this study, water and spermatozoa parameters were evaluated during the reproduction season, as well as the optimal conditions for the retention of the second polar body in Salmo coruhensis eggs obtained with heat‐shock timing, intensity, duration and UV irradiation for gynogenetic production. Sperm was diluted 1:20 with lightly modified Hank's Balanced Salt Solution, and sperm manifesting 60% motility was obtained with a UV dose of 3600 kJ/cm2. The highest ratios of eyed‐stage, hatching and larval survival were 89.59 ± 2.18%, 82.86 ± 3.11% and 79.61 ± 1.25% in the experimental group exposed to 28.5 °C heat‐shock respectively. As the shocking temperature increased, the rates of eyed‐stage, hatching and larval survival decreased (p < 0.05). After 3–7 days of fertilization, all haploid individuals died in all experimental groups. As a result of the morphological examination, no difference was found between the gynogen and control groups at larval and juvenile stages. In karyotype analysis, chromosome numbers of control (C), diploid gynogen (G) and triploid (T) group were 2n = 80 and 3n ≈ 120 respectively. Triploid groups showed 1.5 times higher mean erythrocyte diameter and relative DNA content than G and C groups. Moreover, it was determined that sdY, the salmonid male sex‐determining gene, was not found in all groups that were assumed to be gynogens, and all of them were female (100%). These results indicated that all individuals in the gynogen groups were gynogenetic. The protocol developed in this study can contribute to the production of gynogenetic Coruh trout, the production of mono‐sex species, as well as the genetic performance enhancement studies.</abstract><cop>Oxford</cop><pub>Hindawi Limited</pub><doi>10.1111/are.15930</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-1285-0304</orcidid><orcidid>https://orcid.org/0000-0001-9986-0868</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals |
| subjects | Biological fertilization Chromosomes Coruh trout Deoxyribonucleic acid Diploids Diploidy DNA erythrocyte Erythrocytes Fertilization flow cytometry Freshwater fishes Gynogenesis Haploidy Hatching Irradiation Juveniles Karyotypes Larvae Meiosis Saline solutions Salmo coruhensis sdY gene Sex Sperm Spermatozoa Survival Trout Ultraviolet radiation |
| title | Improved induction of meiotic gynogenesis in Coruh trout, Salmo coruhensis |
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