The Influence of Yarrowia lipolytica Glycosylation on the Biochemical Properties and Oligomerization of Heterologous Invertase

Invertases are important enzymes used in the food industry. Despite many studies on the invertase-encoding SUC2 gene expression in the industrial yeast Yarrowia lipolytica, no biochemical characteristics of this enzyme expressed as heterologous protein have been provided. Here, two isoforms of extra...

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Veröffentlicht in:	Sustainability 2022-07, Vol.14 (13), p.7926
Hauptverfasser:	Szymański, Kacper, Hapeta, Piotr, Moroz, Paweł, Wąsik, Bartosz, Robak, Małgorzata, Lazar, Zbigniew
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemical characteristics Biochemistry Biosynthesis Carbon Chromatography Drinking water Enzymes Food industry Fructooligosaccharides Galactose Gene expression Glucose Glycan Glycerol Glycosylation Hexamers High performance liquid chromatography Invertase Ion exchange Ion-exchange chromatography Isoforms Liquid chromatography Mannose Metal ions Microorganisms Molecular weight N-glycans Oligomerization Polysaccharides Proteins Saccharomyces cerevisiae Substrate inhibition Substrate specificity SUC2 gene Sucrose Sustainability Yarrowia lipolytica Yeast Yeasts
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