Evaluation of the red & blue LED effects on cutaneous refractory wound healing in male Sprague–Dawley rat using 3 different multi‐drug resistant bacteria

Objectives Photobiomodulation (PBM) is widely used in clinical therapy, and is an effective approach to resist the bacterial infection of the cutaneous wound and modulate the wound healing process. Due to the several detriments of lasers, Red & Blue LED light (RBLL) may be a more viable light source. This study is aimed to evaluate and compare the therapeutic effect of RBLL light on different multi‐drug resistant (MDR) bacteria in vitro and male Sprague–Dawley (SD) rat refractory MDR infection wound model in vivo. Materials and Methods Methicillin‐resistant Staphylococcus aureus (MRSA), Extended‐spectrum β‐lactamases ‐producing Escherichia coli (ESBLs‐Eco), and the MDR Pseudomonas aeruginosa (MDR‐Pae) were employed to evaluate the antibacterial effects of the Blue LED light in vitro. Effects of RBLL on in vivo wound healing were evaluated by analyzing time to closure, wound score, semi‐quantitative test for bacterial culture, histopathological examination and Masson staining of skin tissue, immunohistochemical (IHC) staining, and western blot analysis (WB) of wound tissue. Results Blue LED light inhibited MRSA, ESBLs‐Eco, and MDR‐Pae in vitro study. In vivo, RBLL accelerated wound healing, reduced levels of pathogenic bacteria on the wound surface while increasing the blood supply to the wound surface and inhibiting the excessive inflammatory response. Conclusion RBLL showed a great potential gain for the treatment of MDR bacterial infected wounds, suggesting PBM therapy is an inexpensive, convenient, pain‐free, and safe therapeutic intervention for refractory MDR infection wounds.