Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency
Purpose The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization. Methods According to the main components...
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| Veröffentlicht in: | Annals of microbiology 2020-10, Vol.70 (1), Article 62 |
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| creator | Gong, Xiujie Zou, Hongtao Qian, Chunrong Yu, Yang Hao, Yubo Li, Liang Wang, Qiuju Jiang, Yubo Ma, Juntao |
| description | Purpose
The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization.
Methods
According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Result
The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain
Streptomyces
sp. G1
T
has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain
Streptomyces
sp. G2
T
has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain
Streptomyces
sp. G3
T
to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 °C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Conclusion
Streptomyces
sp. G1
T
,
Streptomyces
sp. G2
T
, and
Streptomyces
sp. G3
T
screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources. |
| doi_str_mv | 10.1186/s13213-020-01601-9 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_journals_2670510821</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A788346285</galeid><sourcerecordid>A788346285</sourcerecordid><originalsourceid>FETCH-LOGICAL-c464t-20eb9fc12d2523fb5c76eee2321b2e7608a329cb0f0302376a2946d818f181703</originalsourceid><addsrcrecordid>eNp9kU1r3DAQhk1JoUnaP9CToacevB19WJaOYUnaQKDQj7OQ5dGisLFSSSbZf9_JulD2EoSQmHle8Y7epvnIYMOYVl8KE5yJDjh0wBSwzrxpzqlhOg5SntG9N9BJrti75qKUewBlpJHnDW7TXGpefI1pblNo49yWWJd2wl12kzuWR-cr5uhe-j5lImp2T62bJ9pufyixHKW1nMgwhOgjzv7wvnkb3L7gh3_nZfP75vrX9lt39_3r7fbqrvNSyUpecTTBMz7xnosw9n5QiMhptpHjoEA7wY0fIYAALgbluJFq0kwHptkA4rL5tL77mNOfBUu192nJZLFYrgboGWj6ptcoOYjBgDSKqM1K7dwebZxDoqE9rQkfok8zhkj1q0FrIRXXPQk-nwiIqfhcd24pxd7-_HHK8pX1OZWSMdjHHB9cPlgG9iVRuyZqKVF7TNQaEolVVAied5j_-35F9RfDaaF7</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2670510821</pqid></control><display><type>article</type><title>Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency</title><source>DOAJ Directory of Open Access Journals</source><source>Springer Nature OA Free Journals</source><source>Springer Nature - Complete Springer Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Gong, Xiujie ; Zou, Hongtao ; Qian, Chunrong ; Yu, Yang ; Hao, Yubo ; Li, Liang ; Wang, Qiuju ; Jiang, Yubo ; Ma, Juntao</creator><creatorcontrib>Gong, Xiujie ; Zou, Hongtao ; Qian, Chunrong ; Yu, Yang ; Hao, Yubo ; Li, Liang ; Wang, Qiuju ; Jiang, Yubo ; Ma, Juntao</creatorcontrib><description>Purpose
The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization.
Methods
According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Result
The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain
Streptomyces
sp. G1
T
has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain
Streptomyces
sp. G2
T
has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain
Streptomyces
sp. G3
T
to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 °C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Conclusion
Streptomyces
sp. G1
T
,
Streptomyces
sp. G2
T
, and
Streptomyces
sp. G3
T
screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources.</description><identifier>ISSN: 1590-4261</identifier><identifier>EISSN: 1869-2044</identifier><identifier>DOI: 10.1186/s13213-020-01601-9</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Analysis ; Applied Microbiology ; Bacteria ; Biodegradation ; Biomedical and Life Sciences ; Biotechnology ; Cellulose ; Corn ; Decomposition ; Efficiency ; Enzymatic activity ; Enzymes ; Fourier analysis ; Hemicellulose ; Humus ; Inoculation ; Life Sciences ; Lignin ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mycology ; Original Article ; Scanning electron microscopy ; Strains (organisms) ; Straw ; Streptomyces</subject><ispartof>Annals of microbiology, 2020-10, Vol.70 (1), Article 62</ispartof><rights>The Author(s) 2020</rights><rights>COPYRIGHT 2020 Springer</rights><rights>The Author(s) 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-20eb9fc12d2523fb5c76eee2321b2e7608a329cb0f0302376a2946d818f181703</citedby><cites>FETCH-LOGICAL-c464t-20eb9fc12d2523fb5c76eee2321b2e7608a329cb0f0302376a2946d818f181703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1186/s13213-020-01601-9$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://doi.org/10.1186/s13213-020-01601-9$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,864,27924,27925,41120,41488,42189,42557,51319,51576</link.rule.ids></links><search><creatorcontrib>Gong, Xiujie</creatorcontrib><creatorcontrib>Zou, Hongtao</creatorcontrib><creatorcontrib>Qian, Chunrong</creatorcontrib><creatorcontrib>Yu, Yang</creatorcontrib><creatorcontrib>Hao, Yubo</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Wang, Qiuju</creatorcontrib><creatorcontrib>Jiang, Yubo</creatorcontrib><creatorcontrib>Ma, Juntao</creatorcontrib><title>Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency</title><title>Annals of microbiology</title><addtitle>Ann Microbiol</addtitle><description>Purpose
The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization.
Methods
According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Result
The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain
Streptomyces
sp. G1
T
has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain
Streptomyces
sp. G2
T
has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain
Streptomyces
sp. G3
T
to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 °C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Conclusion
Streptomyces
sp. G1
T
,
Streptomyces
sp. G2
T
, and
Streptomyces
sp. G3
T
screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources.</description><subject>Analysis</subject><subject>Applied Microbiology</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cellulose</subject><subject>Corn</subject><subject>Decomposition</subject><subject>Efficiency</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Fourier analysis</subject><subject>Hemicellulose</subject><subject>Humus</subject><subject>Inoculation</subject><subject>Life Sciences</subject><subject>Lignin</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Original Article</subject><subject>Scanning electron microscopy</subject><subject>Strains (organisms)</subject><subject>Straw</subject><subject>Streptomyces</subject><issn>1590-4261</issn><issn>1869-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kU1r3DAQhk1JoUnaP9CToacevB19WJaOYUnaQKDQj7OQ5dGisLFSSSbZf9_JulD2EoSQmHle8Y7epvnIYMOYVl8KE5yJDjh0wBSwzrxpzqlhOg5SntG9N9BJrti75qKUewBlpJHnDW7TXGpefI1pblNo49yWWJd2wl12kzuWR-cr5uhe-j5lImp2T62bJ9pufyixHKW1nMgwhOgjzv7wvnkb3L7gh3_nZfP75vrX9lt39_3r7fbqrvNSyUpecTTBMz7xnosw9n5QiMhptpHjoEA7wY0fIYAALgbluJFq0kwHptkA4rL5tL77mNOfBUu192nJZLFYrgboGWj6ptcoOYjBgDSKqM1K7dwebZxDoqE9rQkfok8zhkj1q0FrIRXXPQk-nwiIqfhcd24pxd7-_HHK8pX1OZWSMdjHHB9cPlgG9iVRuyZqKVF7TNQaEolVVAied5j_-35F9RfDaaF7</recordid><startdate>20201031</startdate><enddate>20201031</enddate><creator>Gong, Xiujie</creator><creator>Zou, Hongtao</creator><creator>Qian, Chunrong</creator><creator>Yu, Yang</creator><creator>Hao, Yubo</creator><creator>Li, Liang</creator><creator>Wang, Qiuju</creator><creator>Jiang, Yubo</creator><creator>Ma, Juntao</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20201031</creationdate><title>Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency</title><author>Gong, Xiujie ; Zou, Hongtao ; Qian, Chunrong ; Yu, Yang ; Hao, Yubo ; Li, Liang ; Wang, Qiuju ; Jiang, Yubo ; Ma, Juntao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-20eb9fc12d2523fb5c76eee2321b2e7608a329cb0f0302376a2946d818f181703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analysis</topic><topic>Applied Microbiology</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cellulose</topic><topic>Corn</topic><topic>Decomposition</topic><topic>Efficiency</topic><topic>Enzymatic activity</topic><topic>Enzymes</topic><topic>Fourier analysis</topic><topic>Hemicellulose</topic><topic>Humus</topic><topic>Inoculation</topic><topic>Life Sciences</topic><topic>Lignin</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Original Article</topic><topic>Scanning electron microscopy</topic><topic>Strains (organisms)</topic><topic>Straw</topic><topic>Streptomyces</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gong, Xiujie</creatorcontrib><creatorcontrib>Zou, Hongtao</creatorcontrib><creatorcontrib>Qian, Chunrong</creatorcontrib><creatorcontrib>Yu, Yang</creatorcontrib><creatorcontrib>Hao, Yubo</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Wang, Qiuju</creatorcontrib><creatorcontrib>Jiang, Yubo</creatorcontrib><creatorcontrib>Ma, Juntao</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Annals of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gong, Xiujie</au><au>Zou, Hongtao</au><au>Qian, Chunrong</au><au>Yu, Yang</au><au>Hao, Yubo</au><au>Li, Liang</au><au>Wang, Qiuju</au><au>Jiang, Yubo</au><au>Ma, Juntao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency</atitle><jtitle>Annals of microbiology</jtitle><stitle>Ann Microbiol</stitle><date>2020-10-31</date><risdate>2020</risdate><volume>70</volume><issue>1</issue><artnum>62</artnum><issn>1590-4261</issn><eissn>1869-2044</eissn><abstract>Purpose
The highly efficient degradation bacteria were selected from the humus from the very cold straw in China for many years to construct the in situ degradation bacteria, and the degradation efficiency of corn straw was determined by process optimization.
Methods
According to the main components of corn straw, through morphological, physiological, and biochemical screening, three highly efficient complementary degradation strains were selected to construct the compound flora, and the degradation efficiency was analyzed by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Result
The corn straw selected in this paper is mainly composed of cellulose (31.99%), hemicellulose (25.33%), and lignin (14.67%). Through the determination of enzyme activity, strain
Streptomyces
sp. G1
T
has high decomposition ability to cellulose and hemicellulose but weak utilization ability to lignin; strain
Streptomyces
sp. G2
T
has the strongest decomposition ability to cellulose and hemicellulose among the three strains. The decomposition ability of strain
Streptomyces
sp. G3
T
to lignin was the strongest among the three strains. Therefore, by compounding the three strains, the decomposition ability has been greatly improved. The optimal process conditions obtained by single factor and response surface method are as follows: pH is 7, temperature is 30 °C, inoculation amount is 5%, rotational speed is 210 rpm, and the weight loss rate of straw is 60.55% after decomposing for 7 days. A large amount of degradation of corn straw can be seen by Fourier transform infrared spectrometer, field emission scanning electron microscope, and X-ray diffractometer.
Conclusion
Streptomyces
sp. G1
T
,
Streptomyces
sp. G2
T
, and
Streptomyces
sp. G3
T
screened from straw humus in very cold areas were used to construct in situ degradation bacteria, which had good straw degradation activity and had the potential to be used for straw treatment in cold areas after harvest. This characteristic makes the complex bacteria become a strong competitive candidate for industrial production, and it is also an effective biotechnology in line with the current recycling of resources.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1186/s13213-020-01601-9</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | Annals of microbiology, 2020-10, Vol.70 (1), Article 62 |
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| language | eng |
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| source | DOAJ Directory of Open Access Journals; Springer Nature OA Free Journals; Springer Nature - Complete Springer Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analysis Applied Microbiology Bacteria Biodegradation Biomedical and Life Sciences Biotechnology Cellulose Corn Decomposition Efficiency Enzymatic activity Enzymes Fourier analysis Hemicellulose Humus Inoculation Life Sciences Lignin Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Mycology Original Article Scanning electron microscopy Strains (organisms) Straw Streptomyces |
| title | Construction of in situ degradation bacteria of corn straw and analysis of its degradation efficiency |
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