GC–MS-Based Metabolites Profiling, In Vitro Antioxidant, Anticancer, and Antimicrobial Properties of Different Solvent Extracts from the Botanical Parts of Micromeria fruticosa (Lamiaceae)
The present study assessed the metabolites and bioactivities of Micromeria fruticosa plant parts extracted with various solvents (ethanol, n-hexane, and water) through the steeping extraction method. Thereafter, the extracts were analyzed using GC-MS. Moreover, the extracts were tested for their ant...
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| Veröffentlicht in: | Processes 2022-05, Vol.10 (5), p.1016 |
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| creator | Al-Nuri, Mohammad Abu-Reidah, Ibrahim M. Alhajeh, Anwar A. Omar, Ghadeer Adwan, Ghaleb Warad, Ismail |
| description | The present study assessed the metabolites and bioactivities of Micromeria fruticosa plant parts extracted with various solvents (ethanol, n-hexane, and water) through the steeping extraction method. Thereafter, the extracts were analyzed using GC-MS. Moreover, the extracts were tested for their antioxidant, antimicrobial, and antitumor activities. The quali-quantitative analysis of Micromeria fruticosa crude extracts revealed the occurrence of 27 secondary metabolites. Some major bioactives identified were menthone, oleamide, pulegone, and menthol. Numerous antioxidant minerals, viz., Fe, Zn, and Mn, were present. The water extract of leaves exhibited the highest DPPH scavenging activity (89.73%), followed by the water extract of flowers (80.07%) at 100 μg/mL. The stems’ water extract demonstrated greater antimicrobial activity against all the bacteria species tested. The ethanolic leaf and aqueous stem extracts exhibited strong activity against C. albicans and E. coli. Flowers’ aqueous extract demonstrated the highest cytostatic effect on the colon cell line by reducing viability, followed by the leaves’ ethanol extract. The extraction solvents influenced the recovery of phytocompounds, and the highest pharmacological activities of the different extracts could be correlated to the presence of additional bioactives. Our results suggest that the Micromeria fruticosa plant is a favorable source of natural products with promising properties for potential nutraceutical and functional food applications. |
| doi_str_mv | 10.3390/pr10051016 |
| format | Article |
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Thereafter, the extracts were analyzed using GC-MS. Moreover, the extracts were tested for their antioxidant, antimicrobial, and antitumor activities. The quali-quantitative analysis of Micromeria fruticosa crude extracts revealed the occurrence of 27 secondary metabolites. Some major bioactives identified were menthone, oleamide, pulegone, and menthol. Numerous antioxidant minerals, viz., Fe, Zn, and Mn, were present. The water extract of leaves exhibited the highest DPPH scavenging activity (89.73%), followed by the water extract of flowers (80.07%) at 100 μg/mL. The stems’ water extract demonstrated greater antimicrobial activity against all the bacteria species tested. The ethanolic leaf and aqueous stem extracts exhibited strong activity against C. albicans and E. coli. Flowers’ aqueous extract demonstrated the highest cytostatic effect on the colon cell line by reducing viability, followed by the leaves’ ethanol extract. The extraction solvents influenced the recovery of phytocompounds, and the highest pharmacological activities of the different extracts could be correlated to the presence of additional bioactives. Our results suggest that the Micromeria fruticosa plant is a favorable source of natural products with promising properties for potential nutraceutical and functional food applications.</description><identifier>ISSN: 2227-9717</identifier><identifier>EISSN: 2227-9717</identifier><identifier>DOI: 10.3390/pr10051016</identifier><language>eng</language><publisher>Basel: MDPI AG</publisher><subject>Anticancer properties ; Antiinfectives and antibacterials ; Antimicrobial activity ; Antioxidants ; Bacteria ; Biological activity ; Colon ; Diabetes ; E coli ; Ethanol ; Experiments ; Flowers ; Flowers & plants ; Folk medicine ; Functional foods & nutraceuticals ; Herbal medicine ; Hexanes ; Inflammation ; Leaves ; Manganese ; Medicine ; Menthol ; Menthone ; Metabolites ; Micromeria ; Microorganisms ; Minerals ; n-Hexane ; Natural products ; Oleamide ; Plant extracts ; Pulegone ; Respiratory system ; Scavenging ; Secondary metabolites ; Solvent extraction ; Solvents ; Staphylococcus infections ; Stems</subject><ispartof>Processes, 2022-05, Vol.10 (5), p.1016</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c225t-d886fdd62a9a244c4b879d35815b6d9394af26d300c2f0e31a02c61b8219301d3</citedby><cites>FETCH-LOGICAL-c225t-d886fdd62a9a244c4b879d35815b6d9394af26d300c2f0e31a02c61b8219301d3</cites><orcidid>0000-0002-7477-7854 ; 0000-0002-0335-0140 ; 0000-0001-8853-8961</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Al-Nuri, Mohammad</creatorcontrib><creatorcontrib>Abu-Reidah, Ibrahim M.</creatorcontrib><creatorcontrib>Alhajeh, Anwar A.</creatorcontrib><creatorcontrib>Omar, Ghadeer</creatorcontrib><creatorcontrib>Adwan, Ghaleb</creatorcontrib><creatorcontrib>Warad, Ismail</creatorcontrib><title>GC–MS-Based Metabolites Profiling, In Vitro Antioxidant, Anticancer, and Antimicrobial Properties of Different Solvent Extracts from the Botanical Parts of Micromeria fruticosa (Lamiaceae)</title><title>Processes</title><description>The present study assessed the metabolites and bioactivities of Micromeria fruticosa plant parts extracted with various solvents (ethanol, n-hexane, and water) through the steeping extraction method. 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Our results suggest that the Micromeria fruticosa plant is a favorable source of natural products with promising properties for potential nutraceutical and functional food applications.</description><subject>Anticancer properties</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Antioxidants</subject><subject>Bacteria</subject><subject>Biological activity</subject><subject>Colon</subject><subject>Diabetes</subject><subject>E coli</subject><subject>Ethanol</subject><subject>Experiments</subject><subject>Flowers</subject><subject>Flowers & plants</subject><subject>Folk medicine</subject><subject>Functional foods & nutraceuticals</subject><subject>Herbal medicine</subject><subject>Hexanes</subject><subject>Inflammation</subject><subject>Leaves</subject><subject>Manganese</subject><subject>Medicine</subject><subject>Menthol</subject><subject>Menthone</subject><subject>Metabolites</subject><subject>Micromeria</subject><subject>Microorganisms</subject><subject>Minerals</subject><subject>n-Hexane</subject><subject>Natural products</subject><subject>Oleamide</subject><subject>Plant extracts</subject><subject>Pulegone</subject><subject>Respiratory system</subject><subject>Scavenging</subject><subject>Secondary metabolites</subject><subject>Solvent extraction</subject><subject>Solvents</subject><subject>Staphylococcus infections</subject><subject>Stems</subject><issn>2227-9717</issn><issn>2227-9717</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpNUUlKBDEULURBUTeeIOBGpUszdKWqltq2A3Sj4LAtfmXQSFXSJmnRnXfwPh7Gk5huBf2b9z-84cPLsh2CDxmr8dHME4wLgglfyTYopWVel6Rc_bevZ9shPOE0NWFVwTeyz_PR1_vH9CY_gaAkmqoIretMVAFde6dNZ-zDAF1adG-id-jYRuNejQQbB8tDgBXKDxBYubx7I7xrDXQL-Uz5aJKT0-jUaK28shHduO5lgePX6EHEgLR3PYqPCp24CDY5Ji34uJRNF3a98gYSbZ7iXAC0N4HegFCg9reyNQ1dUNu_uJndnY1vRxf55Or8cnQ8yQWlRcxlVXEtJadQAx0OxbCtylqyoiJFy2XN6iFoyiXDWFCNFSOAqeCkrSipGSaSbWa7P74z757nKsTmyc29TZEN5SVmBeeUJdbBDys9HYJXupl504N_awhuFhU1fxWxb--7hjY</recordid><startdate>20220519</startdate><enddate>20220519</enddate><creator>Al-Nuri, Mohammad</creator><creator>Abu-Reidah, Ibrahim M.</creator><creator>Alhajeh, Anwar A.</creator><creator>Omar, Ghadeer</creator><creator>Adwan, Ghaleb</creator><creator>Warad, Ismail</creator><general>MDPI AG</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>LK8</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0002-7477-7854</orcidid><orcidid>https://orcid.org/0000-0002-0335-0140</orcidid><orcidid>https://orcid.org/0000-0001-8853-8961</orcidid></search><sort><creationdate>20220519</creationdate><title>GC–MS-Based Metabolites Profiling, In Vitro Antioxidant, Anticancer, and Antimicrobial Properties of Different Solvent Extracts from the Botanical Parts of Micromeria fruticosa (Lamiaceae)</title><author>Al-Nuri, Mohammad ; 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Thereafter, the extracts were analyzed using GC-MS. Moreover, the extracts were tested for their antioxidant, antimicrobial, and antitumor activities. The quali-quantitative analysis of Micromeria fruticosa crude extracts revealed the occurrence of 27 secondary metabolites. Some major bioactives identified were menthone, oleamide, pulegone, and menthol. Numerous antioxidant minerals, viz., Fe, Zn, and Mn, were present. The water extract of leaves exhibited the highest DPPH scavenging activity (89.73%), followed by the water extract of flowers (80.07%) at 100 μg/mL. The stems’ water extract demonstrated greater antimicrobial activity against all the bacteria species tested. The ethanolic leaf and aqueous stem extracts exhibited strong activity against C. albicans and E. coli. Flowers’ aqueous extract demonstrated the highest cytostatic effect on the colon cell line by reducing viability, followed by the leaves’ ethanol extract. The extraction solvents influenced the recovery of phytocompounds, and the highest pharmacological activities of the different extracts could be correlated to the presence of additional bioactives. Our results suggest that the Micromeria fruticosa plant is a favorable source of natural products with promising properties for potential nutraceutical and functional food applications.</abstract><cop>Basel</cop><pub>MDPI AG</pub><doi>10.3390/pr10051016</doi><orcidid>https://orcid.org/0000-0002-7477-7854</orcidid><orcidid>https://orcid.org/0000-0002-0335-0140</orcidid><orcidid>https://orcid.org/0000-0001-8853-8961</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Anticancer properties Antiinfectives and antibacterials Antimicrobial activity Antioxidants Bacteria Biological activity Colon Diabetes E coli Ethanol Experiments Flowers Flowers & plants Folk medicine Functional foods & nutraceuticals Herbal medicine Hexanes Inflammation Leaves Manganese Medicine Menthol Menthone Metabolites Micromeria Microorganisms Minerals n-Hexane Natural products Oleamide Plant extracts Pulegone Respiratory system Scavenging Secondary metabolites Solvent extraction Solvents Staphylococcus infections Stems |
| title | GC–MS-Based Metabolites Profiling, In Vitro Antioxidant, Anticancer, and Antimicrobial Properties of Different Solvent Extracts from the Botanical Parts of Micromeria fruticosa (Lamiaceae) |
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