Integrated transcriptomic and metabolomic analyses of Caucasian clover (Trifolium ambiguum Bieb.) in response to freezing stress
Caucasian clover ( Trifolium ambiguum Bieb.) is a perennial leguminous herbage with unique properties, such as resistance to cold, drought, and grazing. However, the response of Caucasian clover to freezing stress based on gene expression level and metabolite accumulation is unknown. This study perf...
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| Veröffentlicht in: | Brazilian Journal of Botany 2022-06, Vol.45 (2), p.573-585 |
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| creator | Mou, Dan Li, Zongren Liu, Wenhui Liang, Guoling Jia, Zhifeng Yu, Hongyan Wang, Jiuluan Ou, Weiyou Liu, Kai Yao, Xixi Zhan, Yu Ma, Geliang Kazhuo, Cairang Li, Xilai Xie, Jiuxiang |
| description | Caucasian clover (
Trifolium ambiguum
Bieb.) is a perennial leguminous herbage with unique properties, such as resistance to cold, drought, and grazing. However, the response of Caucasian clover to freezing stress based on gene expression level and metabolite accumulation is unknown. This study performed transcriptomic and metabolomic analyses to investigate the response mechanism of Caucasian clover under freezing stress. Transcriptome analysis detected 6792 differentially expressed genes (DEGs) in Caucasian clover under freezing stress. These DEGs were significantly enriched in carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of secondary metabolites. Meanwhile, 139 differentially accumulated metabolites (DAMs) were identified in Caucasian clover under freezing stress. These DAMs were mainly enriched in amino acid metabolism and biosynthesis of secondary metabolites. Furthermore, the gene-to-metabolite network was used to determine the genes and metabolites involved in polyphenol biosynthesis and osmoprotectants metabolism. Therefore, this study provides insights into the complex regulatory strategy of Caucasian clover response to freezing stress. |
| doi_str_mv | 10.1007/s40415-022-00786-4 |
| format | Article |
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Trifolium ambiguum
Bieb.) is a perennial leguminous herbage with unique properties, such as resistance to cold, drought, and grazing. However, the response of Caucasian clover to freezing stress based on gene expression level and metabolite accumulation is unknown. This study performed transcriptomic and metabolomic analyses to investigate the response mechanism of Caucasian clover under freezing stress. Transcriptome analysis detected 6792 differentially expressed genes (DEGs) in Caucasian clover under freezing stress. These DEGs were significantly enriched in carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of secondary metabolites. Meanwhile, 139 differentially accumulated metabolites (DAMs) were identified in Caucasian clover under freezing stress. These DAMs were mainly enriched in amino acid metabolism and biosynthesis of secondary metabolites. Furthermore, the gene-to-metabolite network was used to determine the genes and metabolites involved in polyphenol biosynthesis and osmoprotectants metabolism. Therefore, this study provides insights into the complex regulatory strategy of Caucasian clover response to freezing stress.</description><identifier>ISSN: 1806-9959</identifier><identifier>ISSN: 0100-8404</identifier><identifier>EISSN: 1806-9959</identifier><identifier>DOI: 10.1007/s40415-022-00786-4</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Amino acids ; Analysis ; Beans ; Biochemistry & Physiology - Original Article ; Biomedical and Life Sciences ; Biosynthesis ; Botany ; Carbohydrate metabolism ; Carbohydrates ; Drought ; Energy metabolism ; Freezing ; Gene expression ; Genes ; Legumes ; Life Sciences ; Lipid metabolism ; Lipids ; Low temperature resistance ; Metabolism ; Metabolites ; Metabolomics ; Mimosaceae ; Osmoprotectants ; Plant metabolites ; Plant Systematics/Taxonomy/Biogeography ; Secondary metabolites ; Transcriptomes ; Transcriptomics ; Trifolium ambiguum ; White people</subject><ispartof>Brazilian Journal of Botany, 2022-06, Vol.45 (2), p.573-585</ispartof><rights>The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022</rights><rights>COPYRIGHT 2022 Springer</rights><rights>The Author(s), under exclusive licence to Botanical Society of Sao Paulo 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-65151149c3692b93f33a11eb55ca60653b531aa2ede5a2822ce9b761760920ed3</citedby><cites>FETCH-LOGICAL-c358t-65151149c3692b93f33a11eb55ca60653b531aa2ede5a2822ce9b761760920ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s40415-022-00786-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s40415-022-00786-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Mou, Dan</creatorcontrib><creatorcontrib>Li, Zongren</creatorcontrib><creatorcontrib>Liu, Wenhui</creatorcontrib><creatorcontrib>Liang, Guoling</creatorcontrib><creatorcontrib>Jia, Zhifeng</creatorcontrib><creatorcontrib>Yu, Hongyan</creatorcontrib><creatorcontrib>Wang, Jiuluan</creatorcontrib><creatorcontrib>Ou, Weiyou</creatorcontrib><creatorcontrib>Liu, Kai</creatorcontrib><creatorcontrib>Yao, Xixi</creatorcontrib><creatorcontrib>Zhan, Yu</creatorcontrib><creatorcontrib>Ma, Geliang</creatorcontrib><creatorcontrib>Kazhuo, Cairang</creatorcontrib><creatorcontrib>Li, Xilai</creatorcontrib><creatorcontrib>Xie, Jiuxiang</creatorcontrib><title>Integrated transcriptomic and metabolomic analyses of Caucasian clover (Trifolium ambiguum Bieb.) in response to freezing stress</title><title>Brazilian Journal of Botany</title><addtitle>Braz. J. Bot</addtitle><description>Caucasian clover (
Trifolium ambiguum
Bieb.) is a perennial leguminous herbage with unique properties, such as resistance to cold, drought, and grazing. However, the response of Caucasian clover to freezing stress based on gene expression level and metabolite accumulation is unknown. This study performed transcriptomic and metabolomic analyses to investigate the response mechanism of Caucasian clover under freezing stress. Transcriptome analysis detected 6792 differentially expressed genes (DEGs) in Caucasian clover under freezing stress. These DEGs were significantly enriched in carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of secondary metabolites. Meanwhile, 139 differentially accumulated metabolites (DAMs) were identified in Caucasian clover under freezing stress. These DAMs were mainly enriched in amino acid metabolism and biosynthesis of secondary metabolites. Furthermore, the gene-to-metabolite network was used to determine the genes and metabolites involved in polyphenol biosynthesis and osmoprotectants metabolism. Therefore, this study provides insights into the complex regulatory strategy of Caucasian clover response to freezing stress.</description><subject>Amino acids</subject><subject>Analysis</subject><subject>Beans</subject><subject>Biochemistry & Physiology - Original Article</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Botany</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Drought</subject><subject>Energy metabolism</subject><subject>Freezing</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Low temperature resistance</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Mimosaceae</subject><subject>Osmoprotectants</subject><subject>Plant metabolites</subject><subject>Plant Systematics/Taxonomy/Biogeography</subject><subject>Secondary metabolites</subject><subject>Transcriptomes</subject><subject>Transcriptomics</subject><subject>Trifolium ambiguum</subject><subject>White people</subject><issn>1806-9959</issn><issn>0100-8404</issn><issn>1806-9959</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9UU2L1jAQDqLg-uof8BTwooe-5qNJm-P6ou7Cgpf1HKbptGRpk9ckFdaTP92sXdDTMgPzwfMMM_MQ8pazI2es-5hb1nLVMCGaWva6aZ-RC94z3RijzPP_8pfkVc53jIlOduaC_L4OBecEBUdaEoTskj-XuHpHIYx0xQJDXB5rWO4zZhoneoLNQfYQqFviT0z0_W3yU1z8tlJYBz9vNfnkcTh-oD7QhPkcQ0ZaIp0S4i8fZppLbefX5MUES8Y3j_FAvn_5fHu6am6-fb0-Xd40Tqq-NFpxxXlrnNRGDEZOUgLnOCjlQDOt5KAkBxA4ogLRC-HQDJ3mnWZGMBzlgbzb555T_LFhLvYubqmelK3QupdCdH1XUccdNcOC1ocp1qe4aiPWF8SAk6_9y45p2csHPxCxE1yKOSec7Dn5FdK95cw-SGN3aWyVxv6VxraVJHdSruAwY_q3yxOsP3LCklU</recordid><startdate>20220601</startdate><enddate>20220601</enddate><creator>Mou, Dan</creator><creator>Li, Zongren</creator><creator>Liu, Wenhui</creator><creator>Liang, Guoling</creator><creator>Jia, Zhifeng</creator><creator>Yu, Hongyan</creator><creator>Wang, Jiuluan</creator><creator>Ou, Weiyou</creator><creator>Liu, Kai</creator><creator>Yao, Xixi</creator><creator>Zhan, Yu</creator><creator>Ma, Geliang</creator><creator>Kazhuo, Cairang</creator><creator>Li, Xilai</creator><creator>Xie, Jiuxiang</creator><general>Springer International Publishing</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>IAO</scope></search><sort><creationdate>20220601</creationdate><title>Integrated transcriptomic and metabolomic analyses of Caucasian clover (Trifolium ambiguum Bieb.) in response to freezing stress</title><author>Mou, Dan ; Li, Zongren ; Liu, Wenhui ; Liang, Guoling ; Jia, Zhifeng ; Yu, Hongyan ; Wang, Jiuluan ; Ou, Weiyou ; Liu, Kai ; Yao, Xixi ; Zhan, Yu ; Ma, Geliang ; Kazhuo, Cairang ; Li, Xilai ; Xie, Jiuxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-65151149c3692b93f33a11eb55ca60653b531aa2ede5a2822ce9b761760920ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Amino acids</topic><topic>Analysis</topic><topic>Beans</topic><topic>Biochemistry & Physiology - Original Article</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Botany</topic><topic>Carbohydrate metabolism</topic><topic>Carbohydrates</topic><topic>Drought</topic><topic>Energy metabolism</topic><topic>Freezing</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Low temperature resistance</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Mimosaceae</topic><topic>Osmoprotectants</topic><topic>Plant metabolites</topic><topic>Plant Systematics/Taxonomy/Biogeography</topic><topic>Secondary metabolites</topic><topic>Transcriptomes</topic><topic>Transcriptomics</topic><topic>Trifolium ambiguum</topic><topic>White people</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mou, Dan</creatorcontrib><creatorcontrib>Li, Zongren</creatorcontrib><creatorcontrib>Liu, Wenhui</creatorcontrib><creatorcontrib>Liang, Guoling</creatorcontrib><creatorcontrib>Jia, Zhifeng</creatorcontrib><creatorcontrib>Yu, Hongyan</creatorcontrib><creatorcontrib>Wang, Jiuluan</creatorcontrib><creatorcontrib>Ou, Weiyou</creatorcontrib><creatorcontrib>Liu, Kai</creatorcontrib><creatorcontrib>Yao, Xixi</creatorcontrib><creatorcontrib>Zhan, Yu</creatorcontrib><creatorcontrib>Ma, Geliang</creatorcontrib><creatorcontrib>Kazhuo, Cairang</creatorcontrib><creatorcontrib>Li, Xilai</creatorcontrib><creatorcontrib>Xie, Jiuxiang</creatorcontrib><collection>CrossRef</collection><collection>Gale Academic OneFile Select</collection><jtitle>Brazilian Journal of Botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mou, Dan</au><au>Li, Zongren</au><au>Liu, Wenhui</au><au>Liang, Guoling</au><au>Jia, Zhifeng</au><au>Yu, Hongyan</au><au>Wang, Jiuluan</au><au>Ou, Weiyou</au><au>Liu, Kai</au><au>Yao, Xixi</au><au>Zhan, Yu</au><au>Ma, Geliang</au><au>Kazhuo, Cairang</au><au>Li, Xilai</au><au>Xie, Jiuxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrated transcriptomic and metabolomic analyses of Caucasian clover (Trifolium ambiguum Bieb.) in response to freezing stress</atitle><jtitle>Brazilian Journal of Botany</jtitle><stitle>Braz. J. Bot</stitle><date>2022-06-01</date><risdate>2022</risdate><volume>45</volume><issue>2</issue><spage>573</spage><epage>585</epage><pages>573-585</pages><issn>1806-9959</issn><issn>0100-8404</issn><eissn>1806-9959</eissn><abstract>Caucasian clover (
Trifolium ambiguum
Bieb.) is a perennial leguminous herbage with unique properties, such as resistance to cold, drought, and grazing. However, the response of Caucasian clover to freezing stress based on gene expression level and metabolite accumulation is unknown. This study performed transcriptomic and metabolomic analyses to investigate the response mechanism of Caucasian clover under freezing stress. Transcriptome analysis detected 6792 differentially expressed genes (DEGs) in Caucasian clover under freezing stress. These DEGs were significantly enriched in carbohydrate and energy metabolism, lipid metabolism, and biosynthesis of secondary metabolites. Meanwhile, 139 differentially accumulated metabolites (DAMs) were identified in Caucasian clover under freezing stress. These DAMs were mainly enriched in amino acid metabolism and biosynthesis of secondary metabolites. Furthermore, the gene-to-metabolite network was used to determine the genes and metabolites involved in polyphenol biosynthesis and osmoprotectants metabolism. Therefore, this study provides insights into the complex regulatory strategy of Caucasian clover response to freezing stress.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><doi>10.1007/s40415-022-00786-4</doi><tpages>13</tpages></addata></record> |
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| subjects | Amino acids Analysis Beans Biochemistry & Physiology - Original Article Biomedical and Life Sciences Biosynthesis Botany Carbohydrate metabolism Carbohydrates Drought Energy metabolism Freezing Gene expression Genes Legumes Life Sciences Lipid metabolism Lipids Low temperature resistance Metabolism Metabolites Metabolomics Mimosaceae Osmoprotectants Plant metabolites Plant Systematics/Taxonomy/Biogeography Secondary metabolites Transcriptomes Transcriptomics Trifolium ambiguum White people |
| title | Integrated transcriptomic and metabolomic analyses of Caucasian clover (Trifolium ambiguum Bieb.) in response to freezing stress |
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