Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS
Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably redu...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2008-09, Vol.10 (5), p.527-537, Article 527 |
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| creator | Li, Hao Miao, Jinlai Cui, Fengxia Li, Guangyou |
| description | Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably reduced sulfhydryl (SH) group content (p < 0.01). Analysis of protein expression profiles by two-dimensional electrophoresis (2-DE) indicated that only 47 protein spots were detected in both control and cupric glutamate treated cells. Three reliable spots were identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as ribulose-bisphosphate carboxylase large subunit precursor, RNA polymerase beta chain, and hypothetical protein, which can be well correlated with cupric glutamate stress. Based on above results, we hypothesize that the extinguishing mechanisms include (1) the cell membrane being damaged by cupric glutamate; (2) cupric glutamate probably induced denaturation and disintegration of intracellular protein, which led to inhibition of cell growth. |
| doi_str_mv | 10.1007/s10126-008-9091-z |
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LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Li, Hao ; Miao, Jinlai ; Cui, Fengxia ; Li, Guangyou</creator><creatorcontrib>Li, Hao ; Miao, Jinlai ; Cui, Fengxia ; Li, Guangyou</creatorcontrib><description>Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably reduced sulfhydryl (SH) group content (p < 0.01). Analysis of protein expression profiles by two-dimensional electrophoresis (2-DE) indicated that only 47 protein spots were detected in both control and cupric glutamate treated cells. Three reliable spots were identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as ribulose-bisphosphate carboxylase large subunit precursor, RNA polymerase beta chain, and hypothetical protein, which can be well correlated with cupric glutamate stress. Based on above results, we hypothesize that the extinguishing mechanisms include (1) the cell membrane being damaged by cupric glutamate; (2) cupric glutamate probably induced denaturation and disintegration of intracellular protein, which led to inhibition of cell growth.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-008-9091-z</identifier><identifier>PMID: 18449603</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Alexandrium ; Alexandrium sp. LC3 ; Algae ; Algicides ; Analytical methods ; Animals ; Biomedical and Life Sciences ; Cell Membrane Permeability - drug effects ; Cell membranes ; Clay ; Copper ; Copper - pharmacology ; Cupric glutamate ; Denaturation ; Dinoflagellida - drug effects ; Dinoflagellida - metabolism ; Disintegration ; DNA-directed RNA polymerase ; Electrophoresis ; Electrophoresis, Gel, Two-Dimensional ; Engineering ; Extinguish ; Extinguishing ; Freshwater & Marine Ecology ; Gene Expression Regulation - drug effects ; Glutamic Acid - pharmacology ; Herbicides ; Ionization ; Ions ; Laboratories ; Lasers ; Life Sciences ; Malondialdehyde ; Malondialdehyde - metabolism ; Mass spectrometry ; Mass spectroscopy ; Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry ; Membrane permeability ; Microbiology ; Organisms ; Original Article ; Permeability ; Pesticides - pharmacology ; Plasma membrane permeability ; Prepolymers ; Protein expression ; Proteins ; Proteomics ; Ribulose-bisphosphate carboxylase ; RNA polymerase ; Scientific imaging ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Studies ; Sulfhydryl Compounds - metabolism ; Two-dimensional electrophoresis ; Water quality ; Zoology</subject><ispartof>Marine biotechnology (New York, N.Y.), 2008-09, Vol.10 (5), p.527-537, Article 527</ispartof><rights>Springer Science+Business Media, LLC 2008</rights><rights>Springer Science+Business Media, LLC 2008.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-54d7384c2365f21a543098da342c2c27a48d16fff37b619eb01fa8f2c8568cf3</citedby><cites>FETCH-LOGICAL-c421t-54d7384c2365f21a543098da342c2c27a48d16fff37b619eb01fa8f2c8568cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-008-9091-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-008-9091-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18449603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Hao</creatorcontrib><creatorcontrib>Miao, Jinlai</creatorcontrib><creatorcontrib>Cui, Fengxia</creatorcontrib><creatorcontrib>Li, Guangyou</creatorcontrib><title>Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably reduced sulfhydryl (SH) group content (p < 0.01). Analysis of protein expression profiles by two-dimensional electrophoresis (2-DE) indicated that only 47 protein spots were detected in both control and cupric glutamate treated cells. Three reliable spots were identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as ribulose-bisphosphate carboxylase large subunit precursor, RNA polymerase beta chain, and hypothetical protein, which can be well correlated with cupric glutamate stress. Based on above results, we hypothesize that the extinguishing mechanisms include (1) the cell membrane being damaged by cupric glutamate; (2) cupric glutamate probably induced denaturation and disintegration of intracellular protein, which led to inhibition of cell growth.</description><subject>Alexandrium</subject><subject>Alexandrium sp. LC3</subject><subject>Algae</subject><subject>Algicides</subject><subject>Analytical methods</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Cell membranes</subject><subject>Clay</subject><subject>Copper</subject><subject>Copper - pharmacology</subject><subject>Cupric glutamate</subject><subject>Denaturation</subject><subject>Dinoflagellida - drug effects</subject><subject>Dinoflagellida - metabolism</subject><subject>Disintegration</subject><subject>DNA-directed RNA polymerase</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Engineering</subject><subject>Extinguish</subject><subject>Extinguishing</subject><subject>Freshwater & Marine Ecology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glutamic Acid - pharmacology</subject><subject>Herbicides</subject><subject>Ionization</subject><subject>Ions</subject><subject>Laboratories</subject><subject>Lasers</subject><subject>Life Sciences</subject><subject>Malondialdehyde</subject><subject>Malondialdehyde - metabolism</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry</subject><subject>Membrane permeability</subject><subject>Microbiology</subject><subject>Organisms</subject><subject>Original Article</subject><subject>Permeability</subject><subject>Pesticides - pharmacology</subject><subject>Plasma membrane permeability</subject><subject>Prepolymers</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Ribulose-bisphosphate carboxylase</subject><subject>RNA polymerase</subject><subject>Scientific imaging</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</subject><subject>Studies</subject><subject>Sulfhydryl Compounds - metabolism</subject><subject>Two-dimensional electrophoresis</subject><subject>Water quality</subject><subject>Zoology</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc1u1DAUhSMEoqXwAGzAgrWL_-I4y1E6_ZFm1EWHteVx7ImrJB5sRy3zBrw1HmXUrkBeXEv3O-de-xTFZ4wuMULVj4gRJhwiJGCNagwPb4pzzCiHhFD-9uVOxFnxIcZHlDUVRe-LMywYqzmi58WfplNB6WSCO6jk_Ai8Bc20D06Dm35KalDJgOVzcuNucrHLBayN7tTo4nBkF715VmMb3DSAuL8Eq4aCJ5c6sHnysHWDGWN2VT1Y9kan4PedDya6CLIIrBerqzu4ub8G64ePxTur-mg-nepFsblebppbuLq_uWsWK6gZwQmWrK2oYDq_sLQEq5JRVItWUUZ0PpViosXcWkurLce12SJslbBEi5ILbelF8X223Qf_azIxyUc_hbxglIRzxnnJCM3Ut39SSNSMUXyE8Azp4GMMxsr8bYMKvyVG8hiQnAOSOSB5DEgesubLyXjaDqZ9VZwSyQCZgZhb486E18n_c_06i6zyUu2Ci_LnA0GYIlQTTvKyfwG3x6Rp</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Li, Hao</creator><creator>Miao, Jinlai</creator><creator>Cui, Fengxia</creator><creator>Li, Guangyou</creator><general>New York : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.F</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope></search><sort><creationdate>20080901</creationdate><title>Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. 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Jinlai</au><au>Cui, Fengxia</au><au>Li, Guangyou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>10</volume><issue>5</issue><spage>527</spage><epage>537</epage><pages>527-537</pages><artnum>527</artnum><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Mechanisms by which cupric glutamate, a novel algicide, extinguishes Alexandrium sp. LC3 are shown in this study. We show that cupric glutamate not only stimulated the production of malonaldehyde (MDA) and dramatically promoted cell plasma membrane permeability (p < 0.01) but also remarkably reduced sulfhydryl (SH) group content (p < 0.01). Analysis of protein expression profiles by two-dimensional electrophoresis (2-DE) indicated that only 47 protein spots were detected in both control and cupric glutamate treated cells. Three reliable spots were identified by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) as ribulose-bisphosphate carboxylase large subunit precursor, RNA polymerase beta chain, and hypothetical protein, which can be well correlated with cupric glutamate stress. Based on above results, we hypothesize that the extinguishing mechanisms include (1) the cell membrane being damaged by cupric glutamate; (2) cupric glutamate probably induced denaturation and disintegration of intracellular protein, which led to inhibition of cell growth.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><pmid>18449603</pmid><doi>10.1007/s10126-008-9091-z</doi><tpages>11</tpages></addata></record> |
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| ispartof | Marine biotechnology (New York, N.Y.), 2008-09, Vol.10 (5), p.527-537, Article 527 |
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| subjects | Alexandrium Alexandrium sp. LC3 Algae Algicides Analytical methods Animals Biomedical and Life Sciences Cell Membrane Permeability - drug effects Cell membranes Clay Copper Copper - pharmacology Cupric glutamate Denaturation Dinoflagellida - drug effects Dinoflagellida - metabolism Disintegration DNA-directed RNA polymerase Electrophoresis Electrophoresis, Gel, Two-Dimensional Engineering Extinguish Extinguishing Freshwater & Marine Ecology Gene Expression Regulation - drug effects Glutamic Acid - pharmacology Herbicides Ionization Ions Laboratories Lasers Life Sciences Malondialdehyde Malondialdehyde - metabolism Mass spectrometry Mass spectroscopy Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry Membrane permeability Microbiology Organisms Original Article Permeability Pesticides - pharmacology Plasma membrane permeability Prepolymers Protein expression Proteins Proteomics Ribulose-bisphosphate carboxylase RNA polymerase Scientific imaging Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Studies Sulfhydryl Compounds - metabolism Two-dimensional electrophoresis Water quality Zoology |
| title | Characterization of Cupric Glutamate Extinguishing Mechanism of Alexandrium sp. LC3 with Two-dimensional Electrophoresis and MALDI-TOF MS |
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