Heterologous expression, on-column refolding and characterization of gamma-glutamyl transpeptidase gene from Bacillus altitudinis IHB B1644: A microbial bioresource from Western Himalayas

Several γ-glutamyl compounds produced from gamma-glutamyltranspeptidase (GGT) have alluring features for food, pharmaceutical, and biotechnology applications. GGT from Bacillus altitudinis IHB B1644 was cloned, followed by an expression in pET-47b(+) Escherichia coli BL21(DE3). Recombinant GGT (BaGG...

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Veröffentlicht in:	Process biochemistry (1991) 2022-05, Vol.116, p.126-135
Hauptverfasser:	Sharma, Eshita, Lal, Milan Kumar, Gulati, Arvind, Gulati, Ashu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Bacillus altitudinis Bacillus altitudinis IHB B1644 Biosynthesis Biotechnology Characterization Cloning Dialysis Dilution E coli Ectopic expression Expression Gamma-glutamyltranspeptidase Inclusion bodies Industrial applications Low temperature Microorganisms Molecular weight On-column refolding Proteins γ-Glutamyltransferase
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