Pathology and Molecular Characterization of Eimeria tenella Isolated from Clinically Infected Broiler Chickens in District Lahore, Pakistan
ABSTRACT Coccidiosis caused by Eimeria tenella is a parasitic disease affecting chickens. In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken fol...
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| Veröffentlicht in: | Pakistan journal of zoology 2022-02, Vol.54 (1), p.47 |
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| creator | Sultan, Rizwana Aslam, Asim Tipu, Muhammad Yasin Ur Rehman, Habib Usman, Saba Anjum, Ahsan Imran, Muhammad Saeed Usman, Muhammad Iqbal, Muhammad Zahid |
| description | ABSTRACT Coccidiosis caused by Eimeria tenella is a parasitic disease affecting chickens. In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. haematological examination of the blood demonstrated a decrease in total erythrocytes, packed cell volume, haemoglobin concentration, and red blood cell indices. Differential leukocyte analysis revealed leukocytosis, heterophilia, eosinophilia, monocytosis, and lymphocytosis. Serum biochemistry showed marked elevation in aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. histopathological examination demonstrated degenerative changes, necrosis haemorrhages, and sloughing off epithelial cells of broad folds of caeca, mild lymphoplasmacytic infiltration in the periportal area of the liver and mild depletion of lymphocyte in the bursa of Fabricius. The seven clades of avian Eimeria species strongly support that E. necatrix and E. tenella were closely associated and placed in the same sister clade with high bootstrap support (98%). Our two isolates RSI and RSII showed a homology index of 99.82% (nucleotide level) and 99.47% (amino acid level) with each other. The maximum similarity percentage indicated that RSI and RSII isolates were closely related to strains reported from India and China. This study is the first report on molecular characterization of E. tenella in Pakistan highlighting the pathological potential and distribution of E. tenella. |
| doi_str_mv | 10.17582/journal.pjz/20200622030642 |
| format | Article |
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In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. haematological examination of the blood demonstrated a decrease in total erythrocytes, packed cell volume, haemoglobin concentration, and red blood cell indices. Differential leukocyte analysis revealed leukocytosis, heterophilia, eosinophilia, monocytosis, and lymphocytosis. Serum biochemistry showed marked elevation in aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. histopathological examination demonstrated degenerative changes, necrosis haemorrhages, and sloughing off epithelial cells of broad folds of caeca, mild lymphoplasmacytic infiltration in the periportal area of the liver and mild depletion of lymphocyte in the bursa of Fabricius. The seven clades of avian Eimeria species strongly support that E. necatrix and E. tenella were closely associated and placed in the same sister clade with high bootstrap support (98%). Our two isolates RSI and RSII showed a homology index of 99.82% (nucleotide level) and 99.47% (amino acid level) with each other. The maximum similarity percentage indicated that RSI and RSII isolates were closely related to strains reported from India and China. This study is the first report on molecular characterization of E. tenella in Pakistan highlighting the pathological potential and distribution of E. tenella.</description><identifier>ISSN: 0030-9923</identifier><identifier>DOI: 10.17582/journal.pjz/20200622030642</identifier><language>eng</language><publisher>Lahore: Knowledge Bylanes</publisher><subject>Alanine ; Alanine transaminase ; Albumins ; Alkaline phosphatase ; Amino acids ; Aspartate transaminase ; Biochemistry ; Birds ; Blood ; Blood levels ; Broilers (Poultry) ; Bursa of Fabricius ; Causes of ; Cell size ; Chickens ; Cholesterol ; Coccidiosis ; Creatinine ; Depletion ; Eimeria tenella ; Eosinophilia ; Epithelial cells ; Epithelium ; Erythrocytes ; Gene amplification ; Genetic aspects ; Globulins ; Health aspects ; Hematology ; Hemoglobin ; Hemorrhage ; Histopathology ; Homology ; Identification and classification ; Infections ; Leukocytes ; Leukocytosis ; Lymphocytes ; Lymphocytosis ; Monocytosis ; Mortality ; Necrosis ; Nucleotides ; Parasites ; Parasitic diseases ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Poultry ; Ribosomal DNA ; Sporozoa ; Triglycerides</subject><ispartof>Pakistan journal of zoology, 2022-02, Vol.54 (1), p.47</ispartof><rights>COPYRIGHT 2022 Knowledge Bylanes</rights><rights>(c)2022 Pakistan Journal of Zoology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-da3660273d957fdd88b645ce495081ce5c7170451db1c109c745cf759d2ccc503</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids></links><search><creatorcontrib>Sultan, Rizwana</creatorcontrib><creatorcontrib>Aslam, Asim</creatorcontrib><creatorcontrib>Tipu, Muhammad Yasin</creatorcontrib><creatorcontrib>Ur Rehman, Habib</creatorcontrib><creatorcontrib>Usman, Saba</creatorcontrib><creatorcontrib>Anjum, Ahsan</creatorcontrib><creatorcontrib>Imran, Muhammad Saeed</creatorcontrib><creatorcontrib>Usman, Muhammad</creatorcontrib><creatorcontrib>Iqbal, Muhammad Zahid</creatorcontrib><title>Pathology and Molecular Characterization of Eimeria tenella Isolated from Clinically Infected Broiler Chickens in District Lahore, Pakistan</title><title>Pakistan journal of zoology</title><description>ABSTRACT Coccidiosis caused by Eimeria tenella is a parasitic disease affecting chickens. In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. haematological examination of the blood demonstrated a decrease in total erythrocytes, packed cell volume, haemoglobin concentration, and red blood cell indices. Differential leukocyte analysis revealed leukocytosis, heterophilia, eosinophilia, monocytosis, and lymphocytosis. Serum biochemistry showed marked elevation in aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. histopathological examination demonstrated degenerative changes, necrosis haemorrhages, and sloughing off epithelial cells of broad folds of caeca, mild lymphoplasmacytic infiltration in the periportal area of the liver and mild depletion of lymphocyte in the bursa of Fabricius. The seven clades of avian Eimeria species strongly support that E. necatrix and E. tenella were closely associated and placed in the same sister clade with high bootstrap support (98%). Our two isolates RSI and RSII showed a homology index of 99.82% (nucleotide level) and 99.47% (amino acid level) with each other. The maximum similarity percentage indicated that RSI and RSII isolates were closely related to strains reported from India and China. This study is the first report on molecular characterization of E. tenella in Pakistan highlighting the pathological potential and distribution of E. tenella.</description><subject>Alanine</subject><subject>Alanine transaminase</subject><subject>Albumins</subject><subject>Alkaline phosphatase</subject><subject>Amino acids</subject><subject>Aspartate transaminase</subject><subject>Biochemistry</subject><subject>Birds</subject><subject>Blood</subject><subject>Blood levels</subject><subject>Broilers (Poultry)</subject><subject>Bursa of Fabricius</subject><subject>Causes of</subject><subject>Cell size</subject><subject>Chickens</subject><subject>Cholesterol</subject><subject>Coccidiosis</subject><subject>Creatinine</subject><subject>Depletion</subject><subject>Eimeria tenella</subject><subject>Eosinophilia</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Erythrocytes</subject><subject>Gene amplification</subject><subject>Genetic aspects</subject><subject>Globulins</subject><subject>Health aspects</subject><subject>Hematology</subject><subject>Hemoglobin</subject><subject>Hemorrhage</subject><subject>Histopathology</subject><subject>Homology</subject><subject>Identification and classification</subject><subject>Infections</subject><subject>Leukocytes</subject><subject>Leukocytosis</subject><subject>Lymphocytes</subject><subject>Lymphocytosis</subject><subject>Monocytosis</subject><subject>Mortality</subject><subject>Necrosis</subject><subject>Nucleotides</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase chain reaction</subject><subject>Poultry</subject><subject>Ribosomal DNA</subject><subject>Sporozoa</subject><subject>Triglycerides</subject><issn>0030-9923</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNptUc1uEzEQ3gNIVKXvYKlXktretb0rTiUUiJSKHuBsTcd249RrB9s5pK_AS-P-IFSJmcP8fTOama_rzhldMiVGfrFLhxwhLPe7hwtOOaWSc9pTOfA33Qlt3mKaeP-uOytlR5sMUnA-nnS_b6BuU0h3RwLRkOsULB4CZLLaQgasNvsHqD5Fkhy58nOLgVQbbQhA1iUFqNYQl9NMVsFHjxDCkayjs_hY-JSTD_Zxmsd7GwvxkXz2pWaPlWxgm7L9QG7gvqUgvu_eOgjFnr3Y0-7nl6sfq2-Lzfev69XlZoED6-vCQC8l5ao3k1DOmHG8lYNAO0yCjgytQMUUHQQztwwZnVC1qlNiMhwRBe1Pu_Pnufucfh1sqfrle0VzKdnEGFP8H-oOgtU-ulTbQ2ZfUF8q2nMm5TA01PI_qKbGzh5TtK7d_7rh43MD5lRKtk7vs58hHzWj-onLv9voxqV-zWX_BwQRl-Q</recordid><startdate>20220228</startdate><enddate>20220228</enddate><creator>Sultan, Rizwana</creator><creator>Aslam, Asim</creator><creator>Tipu, Muhammad Yasin</creator><creator>Ur Rehman, Habib</creator><creator>Usman, Saba</creator><creator>Anjum, Ahsan</creator><creator>Imran, Muhammad Saeed</creator><creator>Usman, Muhammad</creator><creator>Iqbal, Muhammad Zahid</creator><general>Knowledge Bylanes</general><general>AsiaNet Pakistan (Pvt) Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SS</scope><scope>7XB</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope></search><sort><creationdate>20220228</creationdate><title>Pathology and Molecular Characterization of Eimeria tenella Isolated from Clinically Infected Broiler Chickens in District Lahore, Pakistan</title><author>Sultan, Rizwana ; 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In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. haematological examination of the blood demonstrated a decrease in total erythrocytes, packed cell volume, haemoglobin concentration, and red blood cell indices. Differential leukocyte analysis revealed leukocytosis, heterophilia, eosinophilia, monocytosis, and lymphocytosis. Serum biochemistry showed marked elevation in aspartate transaminase, alkaline phosphatase and creatinine and a significant decline in alanine transaminase, total protein, total albumin, globulin, triglycerides and cholesterol values. histopathological examination demonstrated degenerative changes, necrosis haemorrhages, and sloughing off epithelial cells of broad folds of caeca, mild lymphoplasmacytic infiltration in the periportal area of the liver and mild depletion of lymphocyte in the bursa of Fabricius. The seven clades of avian Eimeria species strongly support that E. necatrix and E. tenella were closely associated and placed in the same sister clade with high bootstrap support (98%). Our two isolates RSI and RSII showed a homology index of 99.82% (nucleotide level) and 99.47% (amino acid level) with each other. The maximum similarity percentage indicated that RSI and RSII isolates were closely related to strains reported from India and China. This study is the first report on molecular characterization of E. tenella in Pakistan highlighting the pathological potential and distribution of E. tenella.</abstract><cop>Lahore</cop><pub>Knowledge Bylanes</pub><doi>10.17582/journal.pjz/20200622030642</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Alanine Alanine transaminase Albumins Alkaline phosphatase Amino acids Aspartate transaminase Biochemistry Birds Blood Blood levels Broilers (Poultry) Bursa of Fabricius Causes of Cell size Chickens Cholesterol Coccidiosis Creatinine Depletion Eimeria tenella Eosinophilia Epithelial cells Epithelium Erythrocytes Gene amplification Genetic aspects Globulins Health aspects Hematology Hemoglobin Hemorrhage Histopathology Homology Identification and classification Infections Leukocytes Leukocytosis Lymphocytes Lymphocytosis Monocytosis Mortality Necrosis Nucleotides Parasites Parasitic diseases Phylogenetics Phylogeny Polymerase chain reaction Poultry Ribosomal DNA Sporozoa Triglycerides |
| title | Pathology and Molecular Characterization of Eimeria tenella Isolated from Clinically Infected Broiler Chickens in District Lahore, Pakistan |
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