Association of Fok 1 (Rs10735810) Gene Polymorphism with Dental Caries in Pakistani Adolescents
Objective: To determine the association of single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) with dental caries in Pakistani adolescents. Study Design: Case-control study. Place and Duration of Study: Biochemistry Department, Islamic International Medical College, Rawalpin...
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| Veröffentlicht in: | Pakistan Armed Forces medical journal 2022-02, Vol.72 (1), p.231-34 |
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| creator | Karim, Bilal Rahim, Amena Afzal, Muhammad Ashfaq, Sidra Javed, Muhammad Fahad |
| description | Objective: To determine the association of single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) with dental caries in Pakistani adolescents.
Study Design: Case-control study.
Place and Duration of Study: Biochemistry Department, Islamic International Medical College, Rawalpindi in cooperation with Pathology Department, Railway General Hospital, Rawalpindi Pakistan, from Sep 2019 to Aug 2020.
Methodology: A total of 304 participants were included in the study. There were 152 dental caries cases and 152 normal agematched controls. After taking written informed consent, blood samples were collected from each participant. DNA extraction was done by Chelex Method. PCR was carried out to determine the respective allelic frequencies of Fok 1 (rs10735810) genotype using specific primers.
Results: Out of 304 study participants, there were 26 males (17.1%) and 126 females (82.9%) in case-group; and 37 males (24.3%) and 115 females (75.7%) in control-group. No significant association of Fok 1 (rs10735810) genotype with DMFT score was found in dental caries case-group. Regarding genotypic and allelic frequencies, there was no significant association of Fok 1 (rs10735810) genotype with dental caries case-group.
Conclusion: Single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) is not associated with dental caries in Pakistani adolescents. |
| doi_str_mv | 10.51253/pafmj.v72i1.6181 |
| format | Article |
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Study Design: Case-control study.
Place and Duration of Study: Biochemistry Department, Islamic International Medical College, Rawalpindi in cooperation with Pathology Department, Railway General Hospital, Rawalpindi Pakistan, from Sep 2019 to Aug 2020.
Methodology: A total of 304 participants were included in the study. There were 152 dental caries cases and 152 normal agematched controls. After taking written informed consent, blood samples were collected from each participant. DNA extraction was done by Chelex Method. PCR was carried out to determine the respective allelic frequencies of Fok 1 (rs10735810) genotype using specific primers.
Results: Out of 304 study participants, there were 26 males (17.1%) and 126 females (82.9%) in case-group; and 37 males (24.3%) and 115 females (75.7%) in control-group. No significant association of Fok 1 (rs10735810) genotype with DMFT score was found in dental caries case-group. Regarding genotypic and allelic frequencies, there was no significant association of Fok 1 (rs10735810) genotype with dental caries case-group.
Conclusion: Single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) is not associated with dental caries in Pakistani adolescents.</description><identifier>ISSN: 0030-9648</identifier><identifier>EISSN: 2411-8842</identifier><identifier>DOI: 10.51253/pafmj.v72i1.6181</identifier><language>eng</language><publisher>Rawalpindi: Knowledge Bylanes</publisher><subject>Age groups ; Alfacalcidol ; Amino acids ; Calcifediol ; Dental caries ; Enamel ; Ethylenediaminetetraacetic acid ; Fluoridation ; Genes ; Genetic aspects ; Genotype & phenotype ; Polymorphism ; Sample size ; Single nucleotide polymorphisms ; Teenagers ; Teeth ; Toothbrushing ; Vitamin D ; Vitamin deficiency ; Youth</subject><ispartof>Pakistan Armed Forces medical journal, 2022-02, Vol.72 (1), p.231-34</ispartof><rights>COPYRIGHT 2022 Knowledge Bylanes</rights><rights>(c)2022 Pakistan Armed Forces Medical Journal</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Karim, Bilal</creatorcontrib><creatorcontrib>Rahim, Amena</creatorcontrib><creatorcontrib>Afzal, Muhammad</creatorcontrib><creatorcontrib>Ashfaq, Sidra</creatorcontrib><creatorcontrib>Javed, Muhammad Fahad</creatorcontrib><title>Association of Fok 1 (Rs10735810) Gene Polymorphism with Dental Caries in Pakistani Adolescents</title><title>Pakistan Armed Forces medical journal</title><description>Objective: To determine the association of single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) with dental caries in Pakistani adolescents.
Study Design: Case-control study.
Place and Duration of Study: Biochemistry Department, Islamic International Medical College, Rawalpindi in cooperation with Pathology Department, Railway General Hospital, Rawalpindi Pakistan, from Sep 2019 to Aug 2020.
Methodology: A total of 304 participants were included in the study. There were 152 dental caries cases and 152 normal agematched controls. After taking written informed consent, blood samples were collected from each participant. DNA extraction was done by Chelex Method. PCR was carried out to determine the respective allelic frequencies of Fok 1 (rs10735810) genotype using specific primers.
Results: Out of 304 study participants, there were 26 males (17.1%) and 126 females (82.9%) in case-group; and 37 males (24.3%) and 115 females (75.7%) in control-group. No significant association of Fok 1 (rs10735810) genotype with DMFT score was found in dental caries case-group. Regarding genotypic and allelic frequencies, there was no significant association of Fok 1 (rs10735810) genotype with dental caries case-group.
Conclusion: Single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) is not associated with dental caries in Pakistani adolescents.</description><subject>Age groups</subject><subject>Alfacalcidol</subject><subject>Amino acids</subject><subject>Calcifediol</subject><subject>Dental caries</subject><subject>Enamel</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Fluoridation</subject><subject>Genes</subject><subject>Genetic aspects</subject><subject>Genotype & phenotype</subject><subject>Polymorphism</subject><subject>Sample size</subject><subject>Single nucleotide polymorphisms</subject><subject>Teenagers</subject><subject>Teeth</subject><subject>Toothbrushing</subject><subject>Vitamin D</subject><subject>Vitamin deficiency</subject><subject>Youth</subject><issn>0030-9648</issn><issn>2411-8842</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNptkU1LAzEQhoMoWKo_wFvAix62ZpLNJj2W-gmCInoOaTrR1N1NTVbFf2-sggjOHAaGZz5fQg6ATSRwKU7W1neryZviASYNaNgiI14DVFrXfJuMGBOsmja13iX7Oa9YMSl4zeSImFnO0QU7hNjT6Ol5fKZAj-4yMCWkBnZML7BHehvbjy6m9VPIHX0PwxM9xX6wLZ3bFDDT0NNb-xzyYPtAZ8vYYnYFyHtkx9s24_5PHJOH87P7-WV1fXNxNZ9dV44zNVQLcByXXguPAJpDXePCCiabsqTySpRDpuCmCjy6WgFOhVs0ykopbaMXSxRjcvjdd53iyyvmwazia-rLSMObgjENSvxSj7ZFE3ofh2RdF7IzM8WgYYKXX43J5B-q-BK74GKPPpT8nwL4LnAp5pzQm3UKnU0fBpjZKGQ2CpmNQuZLIfEJBpyBZw</recordid><startdate>20220228</startdate><enddate>20220228</enddate><creator>Karim, Bilal</creator><creator>Rahim, Amena</creator><creator>Afzal, Muhammad</creator><creator>Ashfaq, Sidra</creator><creator>Javed, Muhammad Fahad</creator><general>Knowledge Bylanes</general><general>AsiaNet Pakistan (Pvt) Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20220228</creationdate><title>Association of Fok 1 (Rs10735810) Gene Polymorphism with Dental Caries in Pakistani Adolescents</title><author>Karim, Bilal ; Rahim, Amena ; Afzal, Muhammad ; Ashfaq, Sidra ; Javed, Muhammad Fahad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c207t-b1c2edf83fe1182144eba30564057f7388491c971fec471e93cb67a555a68bde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Age groups</topic><topic>Alfacalcidol</topic><topic>Amino acids</topic><topic>Calcifediol</topic><topic>Dental caries</topic><topic>Enamel</topic><topic>Ethylenediaminetetraacetic acid</topic><topic>Fluoridation</topic><topic>Genes</topic><topic>Genetic aspects</topic><topic>Genotype & phenotype</topic><topic>Polymorphism</topic><topic>Sample size</topic><topic>Single nucleotide polymorphisms</topic><topic>Teenagers</topic><topic>Teeth</topic><topic>Toothbrushing</topic><topic>Vitamin D</topic><topic>Vitamin deficiency</topic><topic>Youth</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karim, Bilal</creatorcontrib><creatorcontrib>Rahim, Amena</creatorcontrib><creatorcontrib>Afzal, Muhammad</creatorcontrib><creatorcontrib>Ashfaq, Sidra</creatorcontrib><creatorcontrib>Javed, Muhammad Fahad</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Pakistan Armed Forces medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karim, Bilal</au><au>Rahim, Amena</au><au>Afzal, Muhammad</au><au>Ashfaq, Sidra</au><au>Javed, Muhammad Fahad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of Fok 1 (Rs10735810) Gene Polymorphism with Dental Caries in Pakistani Adolescents</atitle><jtitle>Pakistan Armed Forces medical journal</jtitle><date>2022-02-28</date><risdate>2022</risdate><volume>72</volume><issue>1</issue><spage>231</spage><epage>34</epage><pages>231-34</pages><issn>0030-9648</issn><eissn>2411-8842</eissn><abstract>Objective: To determine the association of single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) with dental caries in Pakistani adolescents.
Study Design: Case-control study.
Place and Duration of Study: Biochemistry Department, Islamic International Medical College, Rawalpindi in cooperation with Pathology Department, Railway General Hospital, Rawalpindi Pakistan, from Sep 2019 to Aug 2020.
Methodology: A total of 304 participants were included in the study. There were 152 dental caries cases and 152 normal agematched controls. After taking written informed consent, blood samples were collected from each participant. DNA extraction was done by Chelex Method. PCR was carried out to determine the respective allelic frequencies of Fok 1 (rs10735810) genotype using specific primers.
Results: Out of 304 study participants, there were 26 males (17.1%) and 126 females (82.9%) in case-group; and 37 males (24.3%) and 115 females (75.7%) in control-group. No significant association of Fok 1 (rs10735810) genotype with DMFT score was found in dental caries case-group. Regarding genotypic and allelic frequencies, there was no significant association of Fok 1 (rs10735810) genotype with dental caries case-group.
Conclusion: Single nucleotide polymorphism of vitamin D receptor gene Fok 1 (rs10735810) is not associated with dental caries in Pakistani adolescents.</abstract><cop>Rawalpindi</cop><pub>Knowledge Bylanes</pub><doi>10.51253/pafmj.v72i1.6181</doi><tpages>-196</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Age groups Alfacalcidol Amino acids Calcifediol Dental caries Enamel Ethylenediaminetetraacetic acid Fluoridation Genes Genetic aspects Genotype & phenotype Polymorphism Sample size Single nucleotide polymorphisms Teenagers Teeth Toothbrushing Vitamin D Vitamin deficiency Youth |
| title | Association of Fok 1 (Rs10735810) Gene Polymorphism with Dental Caries in Pakistani Adolescents |
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