Immobilized penicillin G acylase with enhanced activity and stability using glutaraldehyde‐modified polydopamine‐coated Fe3O4 nanoparticles

In this work, Fe3O4 nanoparticles (NPs) were coated with polydopamine (PDA) to structure Fe3O4@PDA NPs by the spontaneous oxygen‐mediated self‐polymerization of dopamine (DA) in an aqueous solution of pH = 8.5. The fabricated Fe3O4@PDA NPs were grafted by glutaraldehyde to realize the immobilization...

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Veröffentlicht in:	Biotechnology and applied biochemistry 2022-04, Vol.69 (2), p.629-641
Hauptverfasser:	Zhang, Boyuan, Zhou, Yongshan, Liu, Chunli, Abdelrahman Mohammed, Monier Alhadi, Chen, Zhangjun, Chen, Zhenbin
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics Aqueous solutions Catalysis Catalytic activity Dopamine Enzymatic activity Enzyme activity Enzymes Fe3O4 nanoparticles Fourier transforms Glutaraldehyde Immobilization Iron oxides Magnetic measurement Nanoparticles Penicillin Penicillin G acylase pH effects polydopamine Recovery Stability Transmission electron microscopy
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