Effects of Pulsed‐Wave Photobiomodulation Therapy on Human Spermatozoa
Background and Objectives Previous studies reported that photobiomodulation (PBM) positively affects the mitochondrial respiratory chain in sperm, resulting in improved motility and velocity. As laser settings are not yet fully established, the present study aimed at optimizing PBM on human sperm. I...
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| Veröffentlicht in: | Lasers in surgery and medicine 2022-04, Vol.54 (4), p.540-553 |
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| creator | Espey, Burkhard T. Kielwein, Karin Ven, Hans Steger, Klaus Allam, Jean‐Pierre Paradowska‐Dogan, Agnieszka Ven, Katrin |
| description | Background and Objectives
Previous studies reported that photobiomodulation (PBM) positively affects the mitochondrial respiratory chain in sperm, resulting in improved motility and velocity. As laser settings are not yet fully established, the present study aimed at optimizing PBM on human sperm. In addition, possible side‐effects of PBM on sperm DNA fragmentation level and acrosomal integrity have been analyzed.
Study Design/Materials and Methods
A pulsed laser‐probe (wavelength 655 nm, output power 25 mW/cm², impulse duration 200 nanoseconds) was used. Native fresh liquefied semen samples underwent radiation with energy doses of 0 (control), 4, 6, and 10 J/cm². Sperm parameters were assessed at 0, 30, 60, 90, and 120 minutes after radiation using a computer‐assisted sperm analysis system. Motility and velocity of sperm from asthenozoospermic patients (n = 42) and normozoospermic controls (n = 22) were measured. The amount of DNA strand breaks was analyzed using ligation‐mediated quantitative polymerase chain reaction in patients with asthenozoospermia (n = 18) and normozoospermia (n = 13). Post‐irradiance acrosomal integrity was investigated using flow cytometry based on CD46 protein expression (n = 7).
Results
Exposure to laser energy‐doses of 4 and 6 J/cm² improved sperm motility and velocity in asthenozoospermic patients. PBM exhibited no significant effect on DNA fragmentation level and expression of CD46 serving as a biomarker for acrosome integrity.
Conclusion
PBM improves sperm motility parameters by maintaining DNA and acrosome integrity and, therefore, represents a promising new tool for assisted reproductive therapy. In particular, improving sperm motility in asthenozoospermic patients by PBM in future may contribute to increasing the chance for successful intrauterine insemination. The present trial has no clinical registration number, as only in vitro studies were performed. The study was approved by the local ethics committee and performed according to the Declaration of Helsinki. Lasers Surg. Med. © 2021 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC. |
| doi_str_mv | 10.1002/lsm.23399 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2649002437</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2649002437</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3209-415b7afaac7919e4fda68d056377696652d0cc971b36d474670d48eb3fa9aead3</originalsourceid><addsrcrecordid>eNp1kLFOwzAURS0EoqUw8AMoEhND2uc4tesRVYUiFVGpRYyWE9tqqqQudgIqE5_AN_IlGFLYmN4dzrtXOgidY-hjgGRQ-qqfEML5Aepi4DTmGPAh6gIOeQQ86aAT79cAQBJgx6hDCOMJJ6SLphNjdF77yJpo3pReq8_3jyf5oqP5ytY2K2xlVVPKurCbaLnSTm53UYjTppKbaLHVrpK1fbPyFB0ZGf7P9reHHm8my_E0nj3c3o2vZ3Eetnmc4mHGpJEyZxxznRol6UjBkBLGKKd0mCjIc85wRqhKWUoZqHSkM2Ikl1oq0kOXbe_W2edG-1qsbeM2YVIkNOVBR0pYoK5aKnfWe6eN2Lqikm4nMIhvZyI4Ez_OAnuxb2yySqs_8ldSAAYt8FqUevd_k5gt7tvKL_Lxdos</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2649002437</pqid></control><display><type>article</type><title>Effects of Pulsed‐Wave Photobiomodulation Therapy on Human Spermatozoa</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Espey, Burkhard T. ; Kielwein, Karin ; Ven, Hans ; Steger, Klaus ; Allam, Jean‐Pierre ; Paradowska‐Dogan, Agnieszka ; Ven, Katrin</creator><creatorcontrib>Espey, Burkhard T. ; Kielwein, Karin ; Ven, Hans ; Steger, Klaus ; Allam, Jean‐Pierre ; Paradowska‐Dogan, Agnieszka ; Ven, Katrin</creatorcontrib><description>Background and Objectives
Previous studies reported that photobiomodulation (PBM) positively affects the mitochondrial respiratory chain in sperm, resulting in improved motility and velocity. As laser settings are not yet fully established, the present study aimed at optimizing PBM on human sperm. In addition, possible side‐effects of PBM on sperm DNA fragmentation level and acrosomal integrity have been analyzed.
Study Design/Materials and Methods
A pulsed laser‐probe (wavelength 655 nm, output power 25 mW/cm², impulse duration 200 nanoseconds) was used. Native fresh liquefied semen samples underwent radiation with energy doses of 0 (control), 4, 6, and 10 J/cm². Sperm parameters were assessed at 0, 30, 60, 90, and 120 minutes after radiation using a computer‐assisted sperm analysis system. Motility and velocity of sperm from asthenozoospermic patients (n = 42) and normozoospermic controls (n = 22) were measured. The amount of DNA strand breaks was analyzed using ligation‐mediated quantitative polymerase chain reaction in patients with asthenozoospermia (n = 18) and normozoospermia (n = 13). Post‐irradiance acrosomal integrity was investigated using flow cytometry based on CD46 protein expression (n = 7).
Results
Exposure to laser energy‐doses of 4 and 6 J/cm² improved sperm motility and velocity in asthenozoospermic patients. PBM exhibited no significant effect on DNA fragmentation level and expression of CD46 serving as a biomarker for acrosome integrity.
Conclusion
PBM improves sperm motility parameters by maintaining DNA and acrosome integrity and, therefore, represents a promising new tool for assisted reproductive therapy. In particular, improving sperm motility in asthenozoospermic patients by PBM in future may contribute to increasing the chance for successful intrauterine insemination. The present trial has no clinical registration number, as only in vitro studies were performed. The study was approved by the local ethics committee and performed according to the Declaration of Helsinki. Lasers Surg. Med. © 2021 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.</description><identifier>ISSN: 0196-8092</identifier><identifier>EISSN: 1096-9101</identifier><identifier>DOI: 10.1002/lsm.23399</identifier><identifier>PMID: 33792933</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>acrosome ; Asthenozoospermia - genetics ; Asthenozoospermia - radiotherapy ; Biomarkers ; CASA ; CD46 ; CD46 antigen ; Deoxyribonucleic acid ; DNA ; DNA damage ; DNA fragmentation ; Electron transport ; Ethical standards ; Flow Cytometry ; Fragmentation ; Gene expression ; Humans ; Integrity ; Irradiance ; Lasers ; Light therapy ; LMqPCR ; Low-Level Light Therapy ; Male ; male fertility ; Mitochondria ; Motility ; Parameters ; Patients ; photobiomodulation ; Polymerase chain reaction ; Pulsed lasers ; Radiation ; Reproduction ; Semen ; Sperm ; sperm motility ; Sperm Motility - radiation effects ; sperm velocity ; Spermatozoa ; Spermatozoa - metabolism ; Velocity</subject><ispartof>Lasers in surgery and medicine, 2022-04, Vol.54 (4), p.540-553</ispartof><rights>2021 The Authors. published by Wiley Periodicals LLC.</rights><rights>2021 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.</rights><rights>2021. This article is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3209-415b7afaac7919e4fda68d056377696652d0cc971b36d474670d48eb3fa9aead3</citedby><cites>FETCH-LOGICAL-c3209-415b7afaac7919e4fda68d056377696652d0cc971b36d474670d48eb3fa9aead3</cites><orcidid>0000-0002-4511-0065</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flsm.23399$$EPDF$$P50$$Gwiley$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flsm.23399$$EHTML$$P50$$Gwiley$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33792933$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Espey, Burkhard T.</creatorcontrib><creatorcontrib>Kielwein, Karin</creatorcontrib><creatorcontrib>Ven, Hans</creatorcontrib><creatorcontrib>Steger, Klaus</creatorcontrib><creatorcontrib>Allam, Jean‐Pierre</creatorcontrib><creatorcontrib>Paradowska‐Dogan, Agnieszka</creatorcontrib><creatorcontrib>Ven, Katrin</creatorcontrib><title>Effects of Pulsed‐Wave Photobiomodulation Therapy on Human Spermatozoa</title><title>Lasers in surgery and medicine</title><addtitle>Lasers Surg Med</addtitle><description>Background and Objectives
Previous studies reported that photobiomodulation (PBM) positively affects the mitochondrial respiratory chain in sperm, resulting in improved motility and velocity. As laser settings are not yet fully established, the present study aimed at optimizing PBM on human sperm. In addition, possible side‐effects of PBM on sperm DNA fragmentation level and acrosomal integrity have been analyzed.
Study Design/Materials and Methods
A pulsed laser‐probe (wavelength 655 nm, output power 25 mW/cm², impulse duration 200 nanoseconds) was used. Native fresh liquefied semen samples underwent radiation with energy doses of 0 (control), 4, 6, and 10 J/cm². Sperm parameters were assessed at 0, 30, 60, 90, and 120 minutes after radiation using a computer‐assisted sperm analysis system. Motility and velocity of sperm from asthenozoospermic patients (n = 42) and normozoospermic controls (n = 22) were measured. The amount of DNA strand breaks was analyzed using ligation‐mediated quantitative polymerase chain reaction in patients with asthenozoospermia (n = 18) and normozoospermia (n = 13). Post‐irradiance acrosomal integrity was investigated using flow cytometry based on CD46 protein expression (n = 7).
Results
Exposure to laser energy‐doses of 4 and 6 J/cm² improved sperm motility and velocity in asthenozoospermic patients. PBM exhibited no significant effect on DNA fragmentation level and expression of CD46 serving as a biomarker for acrosome integrity.
Conclusion
PBM improves sperm motility parameters by maintaining DNA and acrosome integrity and, therefore, represents a promising new tool for assisted reproductive therapy. In particular, improving sperm motility in asthenozoospermic patients by PBM in future may contribute to increasing the chance for successful intrauterine insemination. The present trial has no clinical registration number, as only in vitro studies were performed. The study was approved by the local ethics committee and performed according to the Declaration of Helsinki. Lasers Surg. Med. © 2021 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.</description><subject>acrosome</subject><subject>Asthenozoospermia - genetics</subject><subject>Asthenozoospermia - radiotherapy</subject><subject>Biomarkers</subject><subject>CASA</subject><subject>CD46</subject><subject>CD46 antigen</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>DNA fragmentation</subject><subject>Electron transport</subject><subject>Ethical standards</subject><subject>Flow Cytometry</subject><subject>Fragmentation</subject><subject>Gene expression</subject><subject>Humans</subject><subject>Integrity</subject><subject>Irradiance</subject><subject>Lasers</subject><subject>Light therapy</subject><subject>LMqPCR</subject><subject>Low-Level Light Therapy</subject><subject>Male</subject><subject>male fertility</subject><subject>Mitochondria</subject><subject>Motility</subject><subject>Parameters</subject><subject>Patients</subject><subject>photobiomodulation</subject><subject>Polymerase chain reaction</subject><subject>Pulsed lasers</subject><subject>Radiation</subject><subject>Reproduction</subject><subject>Semen</subject><subject>Sperm</subject><subject>sperm motility</subject><subject>Sperm Motility - radiation effects</subject><subject>sperm velocity</subject><subject>Spermatozoa</subject><subject>Spermatozoa - metabolism</subject><subject>Velocity</subject><issn>0196-8092</issn><issn>1096-9101</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><sourceid>EIF</sourceid><recordid>eNp1kLFOwzAURS0EoqUw8AMoEhND2uc4tesRVYUiFVGpRYyWE9tqqqQudgIqE5_AN_IlGFLYmN4dzrtXOgidY-hjgGRQ-qqfEML5Aepi4DTmGPAh6gIOeQQ86aAT79cAQBJgx6hDCOMJJ6SLphNjdF77yJpo3pReq8_3jyf5oqP5ytY2K2xlVVPKurCbaLnSTm53UYjTppKbaLHVrpK1fbPyFB0ZGf7P9reHHm8my_E0nj3c3o2vZ3Eetnmc4mHGpJEyZxxznRol6UjBkBLGKKd0mCjIc85wRqhKWUoZqHSkM2Ikl1oq0kOXbe_W2edG-1qsbeM2YVIkNOVBR0pYoK5aKnfWe6eN2Lqikm4nMIhvZyI4Ez_OAnuxb2yySqs_8ldSAAYt8FqUevd_k5gt7tvKL_Lxdos</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Espey, Burkhard T.</creator><creator>Kielwein, Karin</creator><creator>Ven, Hans</creator><creator>Steger, Klaus</creator><creator>Allam, Jean‐Pierre</creator><creator>Paradowska‐Dogan, Agnieszka</creator><creator>Ven, Katrin</creator><general>Wiley Subscription Services, Inc</general><scope>24P</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>M7Z</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-4511-0065</orcidid></search><sort><creationdate>202204</creationdate><title>Effects of Pulsed‐Wave Photobiomodulation Therapy on Human Spermatozoa</title><author>Espey, Burkhard T. ; Kielwein, Karin ; Ven, Hans ; Steger, Klaus ; Allam, Jean‐Pierre ; Paradowska‐Dogan, Agnieszka ; Ven, Katrin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3209-415b7afaac7919e4fda68d056377696652d0cc971b36d474670d48eb3fa9aead3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>acrosome</topic><topic>Asthenozoospermia - genetics</topic><topic>Asthenozoospermia - radiotherapy</topic><topic>Biomarkers</topic><topic>CASA</topic><topic>CD46</topic><topic>CD46 antigen</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>DNA fragmentation</topic><topic>Electron transport</topic><topic>Ethical standards</topic><topic>Flow Cytometry</topic><topic>Fragmentation</topic><topic>Gene expression</topic><topic>Humans</topic><topic>Integrity</topic><topic>Irradiance</topic><topic>Lasers</topic><topic>Light therapy</topic><topic>LMqPCR</topic><topic>Low-Level Light Therapy</topic><topic>Male</topic><topic>male fertility</topic><topic>Mitochondria</topic><topic>Motility</topic><topic>Parameters</topic><topic>Patients</topic><topic>photobiomodulation</topic><topic>Polymerase chain reaction</topic><topic>Pulsed lasers</topic><topic>Radiation</topic><topic>Reproduction</topic><topic>Semen</topic><topic>Sperm</topic><topic>sperm motility</topic><topic>Sperm Motility - radiation effects</topic><topic>sperm velocity</topic><topic>Spermatozoa</topic><topic>Spermatozoa - metabolism</topic><topic>Velocity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Espey, Burkhard T.</creatorcontrib><creatorcontrib>Kielwein, Karin</creatorcontrib><creatorcontrib>Ven, Hans</creatorcontrib><creatorcontrib>Steger, Klaus</creatorcontrib><creatorcontrib>Allam, Jean‐Pierre</creatorcontrib><creatorcontrib>Paradowska‐Dogan, Agnieszka</creatorcontrib><creatorcontrib>Ven, Katrin</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Lasers in surgery and medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Espey, Burkhard T.</au><au>Kielwein, Karin</au><au>Ven, Hans</au><au>Steger, Klaus</au><au>Allam, Jean‐Pierre</au><au>Paradowska‐Dogan, Agnieszka</au><au>Ven, Katrin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Pulsed‐Wave Photobiomodulation Therapy on Human Spermatozoa</atitle><jtitle>Lasers in surgery and medicine</jtitle><addtitle>Lasers Surg Med</addtitle><date>2022-04</date><risdate>2022</risdate><volume>54</volume><issue>4</issue><spage>540</spage><epage>553</epage><pages>540-553</pages><issn>0196-8092</issn><eissn>1096-9101</eissn><abstract>Background and Objectives
Previous studies reported that photobiomodulation (PBM) positively affects the mitochondrial respiratory chain in sperm, resulting in improved motility and velocity. As laser settings are not yet fully established, the present study aimed at optimizing PBM on human sperm. In addition, possible side‐effects of PBM on sperm DNA fragmentation level and acrosomal integrity have been analyzed.
Study Design/Materials and Methods
A pulsed laser‐probe (wavelength 655 nm, output power 25 mW/cm², impulse duration 200 nanoseconds) was used. Native fresh liquefied semen samples underwent radiation with energy doses of 0 (control), 4, 6, and 10 J/cm². Sperm parameters were assessed at 0, 30, 60, 90, and 120 minutes after radiation using a computer‐assisted sperm analysis system. Motility and velocity of sperm from asthenozoospermic patients (n = 42) and normozoospermic controls (n = 22) were measured. The amount of DNA strand breaks was analyzed using ligation‐mediated quantitative polymerase chain reaction in patients with asthenozoospermia (n = 18) and normozoospermia (n = 13). Post‐irradiance acrosomal integrity was investigated using flow cytometry based on CD46 protein expression (n = 7).
Results
Exposure to laser energy‐doses of 4 and 6 J/cm² improved sperm motility and velocity in asthenozoospermic patients. PBM exhibited no significant effect on DNA fragmentation level and expression of CD46 serving as a biomarker for acrosome integrity.
Conclusion
PBM improves sperm motility parameters by maintaining DNA and acrosome integrity and, therefore, represents a promising new tool for assisted reproductive therapy. In particular, improving sperm motility in asthenozoospermic patients by PBM in future may contribute to increasing the chance for successful intrauterine insemination. The present trial has no clinical registration number, as only in vitro studies were performed. The study was approved by the local ethics committee and performed according to the Declaration of Helsinki. Lasers Surg. Med. © 2021 The Authors. Lasers in Surgery and Medicine published by Wiley Periodicals LLC.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>33792933</pmid><doi>10.1002/lsm.23399</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0002-4511-0065</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | acrosome Asthenozoospermia - genetics Asthenozoospermia - radiotherapy Biomarkers CASA CD46 CD46 antigen Deoxyribonucleic acid DNA DNA damage DNA fragmentation Electron transport Ethical standards Flow Cytometry Fragmentation Gene expression Humans Integrity Irradiance Lasers Light therapy LMqPCR Low-Level Light Therapy Male male fertility Mitochondria Motility Parameters Patients photobiomodulation Polymerase chain reaction Pulsed lasers Radiation Reproduction Semen Sperm sperm motility Sperm Motility - radiation effects sperm velocity Spermatozoa Spermatozoa - metabolism Velocity |
| title | Effects of Pulsed‐Wave Photobiomodulation Therapy on Human Spermatozoa |
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