Molecular characterization and SNPs association with growth‐related traits of myosin heavy chains from the pearl oyster Pinctada fucata martensii
Myosin heavy chains (MYHs) are important proteins in muscle growth and contraction, which may potentially enhance shellfish growth. In this study, the MYH gene from Pinctada fucata martensii (PmMYH) was studied. Sequence analysis revealed that it has an open reading frame of 5823 bp that encode 1940...
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| Veröffentlicht in: | Aquaculture research 2022-05, Vol.53 (7), p.2874-2885 |
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| creator | Chen, Qingyue Yu, Wanmei Hao, Ruijuan Yang, Jingmiao Yang, Chuangye Deng, Yuewen Liao, Yongshan |
| description | Myosin heavy chains (MYHs) are important proteins in muscle growth and contraction, which may potentially enhance shellfish growth. In this study, the MYH gene from Pinctada fucata martensii (PmMYH) was studied. Sequence analysis revealed that it has an open reading frame of 5823 bp that encode 1940 polypeptide residues. Analysis of PmMYH showed four conserved domains, namely Myosin_N, MYSc, IQ and Myosin_tail_1. Blast analysis confirmed that PmMYH has a high identity with MYH protein sequences from Crassostrea virginica (87.25%), C. gigas (86.15%), Biomphalaria glabrata (78.8%), Pecten maximus (77.15%), Mizuhopecten yessoensis (75.78%) and Argopecten irradians (77.30%). Expression pattern analysis of PmMYH demonstrated that it is widely expressed in tissues and developmental stages, especially in the adductor muscles and late development stage. Furthermore, PmMYH also expressed significantly higher in individuals selected for fast growth compared with those selected for slow growth, thereby suggesting its role on growth‐related traits. Ninety‐five single nucleotide polymorphisms (SNPs) were identified in the exon region of PmMYH, and 18 of which were significantly associated with growth (p |
| doi_str_mv | 10.1111/are.15802 |
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In this study, the MYH gene from Pinctada fucata martensii (PmMYH) was studied. Sequence analysis revealed that it has an open reading frame of 5823 bp that encode 1940 polypeptide residues. Analysis of PmMYH showed four conserved domains, namely Myosin_N, MYSc, IQ and Myosin_tail_1. Blast analysis confirmed that PmMYH has a high identity with MYH protein sequences from Crassostrea virginica (87.25%), C. gigas (86.15%), Biomphalaria glabrata (78.8%), Pecten maximus (77.15%), Mizuhopecten yessoensis (75.78%) and Argopecten irradians (77.30%). Expression pattern analysis of PmMYH demonstrated that it is widely expressed in tissues and developmental stages, especially in the adductor muscles and late development stage. Furthermore, PmMYH also expressed significantly higher in individuals selected for fast growth compared with those selected for slow growth, thereby suggesting its role on growth‐related traits. Ninety‐five single nucleotide polymorphisms (SNPs) were identified in the exon region of PmMYH, and 18 of which were significantly associated with growth (p < 0.05). Of the 18 SNPs, 2 were also verified in two independent populations. Therefore, PmMYH and its SNPs could be used as for the gene‐assisted selection of P. f. martensii.</description><identifier>ISSN: 1355-557X</identifier><identifier>EISSN: 1365-2109</identifier><identifier>DOI: 10.1111/are.15802</identifier><language>eng</language><publisher>Oxford: Hindawi Limited</publisher><subject>Analysis ; Chains ; Developmental stages ; Growth ; Marine molluscs ; Muscle contraction ; Muscles ; MYH protein ; Myosin ; myosin heavy chain ; Nucleotide sequence ; Nucleotides ; Oysters ; Pattern analysis ; Pearl oysters ; Pinctada fucata martensii ; Polypeptides ; Proteins ; Sequence analysis ; Shellfish ; Single-nucleotide polymorphism ; SNP</subject><ispartof>Aquaculture research, 2022-05, Vol.53 (7), p.2874-2885</ispartof><rights>2022 John Wiley & Sons Ltd</rights><rights>Copyright © 2022 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2972-5e2226219625835c2ff1339d5e88c6f39e185acd70b44398ef8fb498962b224e3</citedby><cites>FETCH-LOGICAL-c2972-5e2226219625835c2ff1339d5e88c6f39e185acd70b44398ef8fb498962b224e3</cites><orcidid>0000-0003-1042-6905 ; 0000-0001-6648-5424 ; 0000-0002-4060-2220</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fare.15802$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fare.15802$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Chen, Qingyue</creatorcontrib><creatorcontrib>Yu, Wanmei</creatorcontrib><creatorcontrib>Hao, Ruijuan</creatorcontrib><creatorcontrib>Yang, Jingmiao</creatorcontrib><creatorcontrib>Yang, Chuangye</creatorcontrib><creatorcontrib>Deng, Yuewen</creatorcontrib><creatorcontrib>Liao, Yongshan</creatorcontrib><title>Molecular characterization and SNPs association with growth‐related traits of myosin heavy chains from the pearl oyster Pinctada fucata martensii</title><title>Aquaculture research</title><description>Myosin heavy chains (MYHs) are important proteins in muscle growth and contraction, which may potentially enhance shellfish growth. In this study, the MYH gene from Pinctada fucata martensii (PmMYH) was studied. Sequence analysis revealed that it has an open reading frame of 5823 bp that encode 1940 polypeptide residues. Analysis of PmMYH showed four conserved domains, namely Myosin_N, MYSc, IQ and Myosin_tail_1. Blast analysis confirmed that PmMYH has a high identity with MYH protein sequences from Crassostrea virginica (87.25%), C. gigas (86.15%), Biomphalaria glabrata (78.8%), Pecten maximus (77.15%), Mizuhopecten yessoensis (75.78%) and Argopecten irradians (77.30%). Expression pattern analysis of PmMYH demonstrated that it is widely expressed in tissues and developmental stages, especially in the adductor muscles and late development stage. Furthermore, PmMYH also expressed significantly higher in individuals selected for fast growth compared with those selected for slow growth, thereby suggesting its role on growth‐related traits. Ninety‐five single nucleotide polymorphisms (SNPs) were identified in the exon region of PmMYH, and 18 of which were significantly associated with growth (p < 0.05). Of the 18 SNPs, 2 were also verified in two independent populations. Therefore, PmMYH and its SNPs could be used as for the gene‐assisted selection of P. f. martensii.</description><subject>Analysis</subject><subject>Chains</subject><subject>Developmental stages</subject><subject>Growth</subject><subject>Marine molluscs</subject><subject>Muscle contraction</subject><subject>Muscles</subject><subject>MYH protein</subject><subject>Myosin</subject><subject>myosin heavy chain</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Oysters</subject><subject>Pattern analysis</subject><subject>Pearl oysters</subject><subject>Pinctada fucata martensii</subject><subject>Polypeptides</subject><subject>Proteins</subject><subject>Sequence analysis</subject><subject>Shellfish</subject><subject>Single-nucleotide polymorphism</subject><subject>SNP</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOwzAQRSMEEqWw4A8ssWKREttx6iwrxEviUfGQ2EVTZ0xcpXGxXaqw4hOQ-EO-hJSwZTYzGp25d3Sj6JAmI9rVCTgcUSETthUNKM9EzGiSb29mIWIhxs-70Z738yShacLpIPq6sTWqVQ2OqAocqIDOvEMwtiHQlOThduoJeG-V6ZdrEyry4uw6VN8fnw5rCFiS4MAET6wmi9Z605AK4a3dSJrGE-3sgoQKyRLB1cS2vnMhU9OoACUQvVIQgCzABWy8MfvRjoba48FfH0ZP52ePp5fx9d3F1enkOlYsH7NYIGMsYzTPmJBcKKY15TwvBUqpMs1zpFKAKsfJLE15LlFLPUtz2fEzxlLkw-io1106-7pCH4q5XbmmsyxYlkrKU0plRx33lHLWe4e6WDrT_doWNCk2mRdd5sVv5h170rNrU2P7P1hM7s_6ix9ERIZa</recordid><startdate>202205</startdate><enddate>202205</enddate><creator>Chen, Qingyue</creator><creator>Yu, Wanmei</creator><creator>Hao, Ruijuan</creator><creator>Yang, Jingmiao</creator><creator>Yang, Chuangye</creator><creator>Deng, Yuewen</creator><creator>Liao, Yongshan</creator><general>Hindawi Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-1042-6905</orcidid><orcidid>https://orcid.org/0000-0001-6648-5424</orcidid><orcidid>https://orcid.org/0000-0002-4060-2220</orcidid></search><sort><creationdate>202205</creationdate><title>Molecular characterization and SNPs association with growth‐related traits of myosin heavy chains from the pearl oyster Pinctada fucata martensii</title><author>Chen, Qingyue ; Yu, Wanmei ; Hao, Ruijuan ; Yang, Jingmiao ; Yang, Chuangye ; Deng, Yuewen ; Liao, Yongshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2972-5e2226219625835c2ff1339d5e88c6f39e185acd70b44398ef8fb498962b224e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Analysis</topic><topic>Chains</topic><topic>Developmental stages</topic><topic>Growth</topic><topic>Marine molluscs</topic><topic>Muscle contraction</topic><topic>Muscles</topic><topic>MYH protein</topic><topic>Myosin</topic><topic>myosin heavy chain</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Oysters</topic><topic>Pattern analysis</topic><topic>Pearl oysters</topic><topic>Pinctada fucata martensii</topic><topic>Polypeptides</topic><topic>Proteins</topic><topic>Sequence analysis</topic><topic>Shellfish</topic><topic>Single-nucleotide polymorphism</topic><topic>SNP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Qingyue</creatorcontrib><creatorcontrib>Yu, Wanmei</creatorcontrib><creatorcontrib>Hao, Ruijuan</creatorcontrib><creatorcontrib>Yang, Jingmiao</creatorcontrib><creatorcontrib>Yang, Chuangye</creatorcontrib><creatorcontrib>Deng, Yuewen</creatorcontrib><creatorcontrib>Liao, Yongshan</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Aquaculture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Qingyue</au><au>Yu, Wanmei</au><au>Hao, Ruijuan</au><au>Yang, Jingmiao</au><au>Yang, Chuangye</au><au>Deng, Yuewen</au><au>Liao, Yongshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization and SNPs association with growth‐related traits of myosin heavy chains from the pearl oyster Pinctada fucata martensii</atitle><jtitle>Aquaculture research</jtitle><date>2022-05</date><risdate>2022</risdate><volume>53</volume><issue>7</issue><spage>2874</spage><epage>2885</epage><pages>2874-2885</pages><issn>1355-557X</issn><eissn>1365-2109</eissn><abstract>Myosin heavy chains (MYHs) are important proteins in muscle growth and contraction, which may potentially enhance shellfish growth. In this study, the MYH gene from Pinctada fucata martensii (PmMYH) was studied. Sequence analysis revealed that it has an open reading frame of 5823 bp that encode 1940 polypeptide residues. Analysis of PmMYH showed four conserved domains, namely Myosin_N, MYSc, IQ and Myosin_tail_1. Blast analysis confirmed that PmMYH has a high identity with MYH protein sequences from Crassostrea virginica (87.25%), C. gigas (86.15%), Biomphalaria glabrata (78.8%), Pecten maximus (77.15%), Mizuhopecten yessoensis (75.78%) and Argopecten irradians (77.30%). Expression pattern analysis of PmMYH demonstrated that it is widely expressed in tissues and developmental stages, especially in the adductor muscles and late development stage. Furthermore, PmMYH also expressed significantly higher in individuals selected for fast growth compared with those selected for slow growth, thereby suggesting its role on growth‐related traits. Ninety‐five single nucleotide polymorphisms (SNPs) were identified in the exon region of PmMYH, and 18 of which were significantly associated with growth (p < 0.05). Of the 18 SNPs, 2 were also verified in two independent populations. Therefore, PmMYH and its SNPs could be used as for the gene‐assisted selection of P. f. martensii.</abstract><cop>Oxford</cop><pub>Hindawi Limited</pub><doi>10.1111/are.15802</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-1042-6905</orcidid><orcidid>https://orcid.org/0000-0001-6648-5424</orcidid><orcidid>https://orcid.org/0000-0002-4060-2220</orcidid></addata></record> |
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| subjects | Analysis Chains Developmental stages Growth Marine molluscs Muscle contraction Muscles MYH protein Myosin myosin heavy chain Nucleotide sequence Nucleotides Oysters Pattern analysis Pearl oysters Pinctada fucata martensii Polypeptides Proteins Sequence analysis Shellfish Single-nucleotide polymorphism SNP |
| title | Molecular characterization and SNPs association with growth‐related traits of myosin heavy chains from the pearl oyster Pinctada fucata martensii |
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