CHOLANGIOCARCINOMA: TUMOR SUPPRESSOR MIR-101-3P AS POTENTIAL DIAGNOSIS AND PROGNOSTIC BIOMARKER

Patients with cholangiocarcinoma (CCA) tumor located in the biliary tract, have low survival due to difficulties in diagnosis in its early stage. In this context, microRNAs with inhibitory potential on tumor proliferation, such as miR-101-3p, are a potential molecular marker for diagnosis and prognosis of CCA. The aim of this study was to evaluate the expression of miR-101-3p in patients with CCA and its relationship with survival, lifestyle habits and comorbidities. 41 patients (SG) (median age: 56 y; 51.2% female) and 21 individuals from the control group (CG) (median age: 39 y; 80.9% female) who underwent cholecystectomy were studied. RNA was extracted from tumor tissue embedded in paraffin in SG and fresh tissue in CG. Expression analysis of miR-101-3p was performed by real-time reverse transcription polymerase chain reaction with significance level assumed for a value of p