Genotoxicity Evaluation of Pectin-Mediated Gold Nanoparticles on Zebrafish Embryos (Danio rerio)
Pectin is a polymer commonly found in the fruit peels. Pectin-mediated gold nanoparticles (AuNPs) were fabricated and characterized by spectroscopic and microscopic analysis. The transmission electron microscopy revealed the presence of polymorphic and polydisperse nanoparticles in the size range of...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2022-04, Vol.58 (2), p.186-194 |
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| creator | Sowmiya, P. Dhas, T. S. Sai, D. N. Jyotsna Anandakumar, N. Kumar, V. G. Ravi, M. Nalini, S. Borgio, J. F. AbdulAzeez, S. Karthick, V. |
| description | Pectin is a polymer commonly found in the fruit peels. Pectin-mediated gold nanoparticles (AuNPs) were fabricated and characterized by spectroscopic and microscopic analysis. The transmission electron microscopy revealed the presence of polymorphic and polydisperse nanoparticles in the size range of 4–18 nm. Cytotoxicity studies using 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl tetrazolium bromide on
Gambusia affinis
skin cell line demonstrated that there is no significant toxicity at lower concentrations. Zebrafish (
Danio rerio
) embryos were used to study the effect and distribution of AuNPs
in vivo
and the toxicological parameters (mortality, heart beats per min and hatching rate) were recorded. A concentration-dependent rise in mortality was also observed in AuNPs-treated embryos. Furthermore, the acridine orange staining was used to assess the genotoxicity of pectin-mediated AuNPs on the zebrafish embryos. The results indicated that these particles did not express toxicity to the zebrafish embryonic development at lower concentrations. |
| doi_str_mv | 10.1134/S0003683822020156 |
| format | Article |
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Gambusia affinis
skin cell line demonstrated that there is no significant toxicity at lower concentrations. Zebrafish (
Danio rerio
) embryos were used to study the effect and distribution of AuNPs
in vivo
and the toxicological parameters (mortality, heart beats per min and hatching rate) were recorded. A concentration-dependent rise in mortality was also observed in AuNPs-treated embryos. Furthermore, the acridine orange staining was used to assess the genotoxicity of pectin-mediated AuNPs on the zebrafish embryos. The results indicated that these particles did not express toxicity to the zebrafish embryonic development at lower concentrations.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683822020156</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biocompatibility ; Biomedical and Life Sciences ; Cytotoxicity ; Danio rerio ; Embryogenesis ; Embryonic growth stage ; Embryos ; Genotoxicity ; Gold ; Hatching ; In vivo methods and tests ; Life Sciences ; Medical Microbiology ; Microbiology ; Microscopic analysis ; Mortality ; Nanoparticles ; Pectin ; Polymers ; Toxicity ; Transmission electron microscopy ; Zebrafish</subject><ispartof>Applied biochemistry and microbiology, 2022-04, Vol.58 (2), p.186-194</ispartof><rights>Pleiades Publishing, Inc. 2022. ISSN 0003-6838, Applied Biochemistry and Microbiology, 2022, Vol. 58, No. 2, pp. 186–194. © Pleiades Publishing, Inc., 2022.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-800dc9b6b2f4da953ddd3dbe4f1dce170024e4df4ace8465b3331f49e5d47bc23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683822020156$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683822020156$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Sowmiya, P.</creatorcontrib><creatorcontrib>Dhas, T. S.</creatorcontrib><creatorcontrib>Sai, D. N.</creatorcontrib><creatorcontrib>Jyotsna</creatorcontrib><creatorcontrib>Anandakumar, N.</creatorcontrib><creatorcontrib>Kumar, V. G.</creatorcontrib><creatorcontrib>Ravi, M.</creatorcontrib><creatorcontrib>Nalini, S.</creatorcontrib><creatorcontrib>Borgio, J. F.</creatorcontrib><creatorcontrib>AbdulAzeez, S.</creatorcontrib><creatorcontrib>Karthick, V.</creatorcontrib><title>Genotoxicity Evaluation of Pectin-Mediated Gold Nanoparticles on Zebrafish Embryos (Danio rerio)</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Pectin is a polymer commonly found in the fruit peels. Pectin-mediated gold nanoparticles (AuNPs) were fabricated and characterized by spectroscopic and microscopic analysis. The transmission electron microscopy revealed the presence of polymorphic and polydisperse nanoparticles in the size range of 4–18 nm. Cytotoxicity studies using 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl tetrazolium bromide on
Gambusia affinis
skin cell line demonstrated that there is no significant toxicity at lower concentrations. Zebrafish (
Danio rerio
) embryos were used to study the effect and distribution of AuNPs
in vivo
and the toxicological parameters (mortality, heart beats per min and hatching rate) were recorded. A concentration-dependent rise in mortality was also observed in AuNPs-treated embryos. Furthermore, the acridine orange staining was used to assess the genotoxicity of pectin-mediated AuNPs on the zebrafish embryos. The results indicated that these particles did not express toxicity to the zebrafish embryonic development at lower concentrations.</description><subject>Biochemistry</subject><subject>Biocompatibility</subject><subject>Biomedical and Life Sciences</subject><subject>Cytotoxicity</subject><subject>Danio rerio</subject><subject>Embryogenesis</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Genotoxicity</subject><subject>Gold</subject><subject>Hatching</subject><subject>In vivo methods and tests</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Microscopic analysis</subject><subject>Mortality</subject><subject>Nanoparticles</subject><subject>Pectin</subject><subject>Polymers</subject><subject>Toxicity</subject><subject>Transmission electron microscopy</subject><subject>Zebrafish</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLAzEUhYMoWKs_wF3AjS5G85o0s5Raq-AL1I2bMZPcaMp0UpNU7L93SgUX4upyOd85l3sQOqTklFIuzh4JIVwqrhgjjNBSbqEBlUQVnDCxjQZruVjru2gvpVm_VlJVA_Q6hS7k8OWNzys8-dTtUmcfOhwcfgCTfVfcgvU6g8XT0Fp8p7uw0DF700LCPfgCTdTOp3c8mTdxFRI-vtCdDzhC9OFkH-043SY4-JlD9Hw5eRpfFTf30-vx-U1hmFS5UIRYUzWyYU5YXZXcWsttA8JRa4COSP8GCOuENqCELBvOOXWigtKKUWMYH6KjTe4iho8lpFzPwjJ2_cmaScEqOipV2VN0Q5kYUorg6kX0cx1XNSX1usj6T5G9h208qWe7N4i_yf-bvgF11nVj</recordid><startdate>20220401</startdate><enddate>20220401</enddate><creator>Sowmiya, P.</creator><creator>Dhas, T. 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F.</creatorcontrib><creatorcontrib>AbdulAzeez, S.</creatorcontrib><creatorcontrib>Karthick, V.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sowmiya, P.</au><au>Dhas, T. S.</au><au>Sai, D. N.</au><au>Jyotsna</au><au>Anandakumar, N.</au><au>Kumar, V. G.</au><au>Ravi, M.</au><au>Nalini, S.</au><au>Borgio, J. F.</au><au>AbdulAzeez, S.</au><au>Karthick, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genotoxicity Evaluation of Pectin-Mediated Gold Nanoparticles on Zebrafish Embryos (Danio rerio)</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2022-04-01</date><risdate>2022</risdate><volume>58</volume><issue>2</issue><spage>186</spage><epage>194</epage><pages>186-194</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Pectin is a polymer commonly found in the fruit peels. Pectin-mediated gold nanoparticles (AuNPs) were fabricated and characterized by spectroscopic and microscopic analysis. The transmission electron microscopy revealed the presence of polymorphic and polydisperse nanoparticles in the size range of 4–18 nm. Cytotoxicity studies using 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl tetrazolium bromide on
Gambusia affinis
skin cell line demonstrated that there is no significant toxicity at lower concentrations. Zebrafish (
Danio rerio
) embryos were used to study the effect and distribution of AuNPs
in vivo
and the toxicological parameters (mortality, heart beats per min and hatching rate) were recorded. A concentration-dependent rise in mortality was also observed in AuNPs-treated embryos. Furthermore, the acridine orange staining was used to assess the genotoxicity of pectin-mediated AuNPs on the zebrafish embryos. The results indicated that these particles did not express toxicity to the zebrafish embryonic development at lower concentrations.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683822020156</doi><tpages>9</tpages></addata></record> |
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| subjects | Biochemistry Biocompatibility Biomedical and Life Sciences Cytotoxicity Danio rerio Embryogenesis Embryonic growth stage Embryos Genotoxicity Gold Hatching In vivo methods and tests Life Sciences Medical Microbiology Microbiology Microscopic analysis Mortality Nanoparticles Pectin Polymers Toxicity Transmission electron microscopy Zebrafish |
| title | Genotoxicity Evaluation of Pectin-Mediated Gold Nanoparticles on Zebrafish Embryos (Danio rerio) |
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