Production and characterization of hybrid nanofiber wound dressing containing Centella asiatica coated silver nanoparticles by mutual electrospinning method

[Display omitted] •Centella asiatica (CA) coated silver nanoparticles (CA-AgNPs) were synthesized with using CA extract as reducing and stabilizing agent.•Poly caprolactone (PCL) and polyethylene oxide (PEO) nanofibers composition containing CA-AgNPs was synthesized by mutual electrospinning method.•The wound healing effect of CA, the antibacterial effect of AgNPs and the mechanical properties of PCL:PEO were used to obtain a new wound dressing material.•The morphological, chemical, thermal, mechanical, surface, antibacterial and cytotoxicity properties of the obtained nanofiber membranes were investigated. The aim of this work is to produce a therapeutic and antimicrobial nanofiber wound dressing material with suitable air permeability, water vapor transmission, water absorption, porosity, thermal and mechanical strength. For this purpose, first of all, Centella asiatica (CA) coated silver nanoparticles (CA-AgNPs) are synthesized with using CA extract as reducing and stabilizing agent. The green synthesized CA-AgNPs are characterized by UV–Vis spectroscopy, transmission electron microscope (TEM), zeta potential and fourier transform infrared (FTIR) spectroscopy measurements. The obtained CA-AgNPs give a single peak in the 420–430 nm range between 200 and 700 nm due to surface plasmon resonance (SPR). The average zeta potential and size of CA-AgNPs is found to be −30.4 mV and 14.8 ± 7.3 nm, respectively. Then, poly caprolactone (PCL) and polyethylene oxide (PEO) nanofibers composition containing CA-AgNPs is synthesized by mutual electrospinning method. Before electrospinning, the PCL solution (12% w/v in chloroform/methanol (70%/30%, v/v)), PEO solution (3.5%, w/v in water) and PEO solutions containing 1%, 5% and 10% (v:v) of CA-AgNPs are prepared. In electrospinning experiments, to synthesis the PCL/PEO nanofibers containing CA-AgNPs, an electrospinning set-up consisting of two high voltage sources, an aluminum rotary roller collector and two syringe pumps (one with a double syringe and the other with a single syringe) is used. To produce the wound dressing materials, PEO solutions containing CA-AgNPs are placed on the double syringe pump and PCL solution is placed on the single syringe pump. The surface and physico-chemical properties of the produced hybrid nanofibers are characterized by field emission scanning electron microscopy (FESEM), energy dispersion spectrometry (EDS), FTIR, X-ray diffractometer (XRD), Brunauer-Emmett-Teller (BET) surface area a