Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein β2 subunit gene and the Epo gene
Abnormal production of erythropoietin (Epo) has been described in several human and murine erythroleukemia. The murine IW32 cell line is derived from an F-MuLV-induced erythroleukemia. An autocrine Epo production due to the rearrangement of one Epo allele has been previously described (Beru et al.,...
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| Veröffentlicht in: | Oncogene 1997-10, Vol.15 (16), p.1995-1999 |
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| container_end_page | 1999 |
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| container_issue | 16 |
| container_start_page | 1995 |
| container_title | Oncogene |
| container_volume | 15 |
| creator | CHRETIEN, S DUPREZ, V MAOUCHE, L GISSELBRECHT, S MAYEUX, P LACOMBE, C |
| description | Abnormal production of erythropoietin (Epo) has been described in several human and murine erythroleukemia. The murine IW32 cell line is derived from an F-MuLV-induced erythroleukemia. An autocrine Epo production due to the rearrangement of one Epo allele has been previously described (Beru et al., 1989). However, the exact mechanism leading to the transcriptional activation of the abnormal Epo gene was unknown. In this study, we show that this deregulated expression results from a deletion within chromosome 5. The Epo gene in the abnormal allele is under the control of the G-protein β2 subunit gene promoter and the expressed mRNA results from the fusion of the non coding exon 1 of the G-protein β2 subunit gene to a truncated Epo exon 1 gene. This resulting abnormal cDNA allows the expression of a normal Epo protein. |
| doi_str_mv | 10.1038/sj.onc.1201364 |
| format | Article |
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The murine IW32 cell line is derived from an F-MuLV-induced erythroleukemia. An autocrine Epo production due to the rearrangement of one Epo allele has been previously described (Beru et al., 1989). However, the exact mechanism leading to the transcriptional activation of the abnormal Epo gene was unknown. In this study, we show that this deregulated expression results from a deletion within chromosome 5. The Epo gene in the abnormal allele is under the control of the G-protein β2 subunit gene promoter and the expressed mRNA results from the fusion of the non coding exon 1 of the G-protein β2 subunit gene to a truncated Epo exon 1 gene. This resulting abnormal cDNA allows the expression of a normal Epo protein.</description><identifier>ISSN: 0950-9232</identifier><identifier>EISSN: 1476-5594</identifier><identifier>DOI: 10.1038/sj.onc.1201364</identifier><language>eng</language><publisher>Basingstoke: Nature Publishing</publisher><subject>Alleles ; Autocrine signalling ; Biological and medical sciences ; Cell physiology ; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes ; Chromosome 5 ; Chromosome deletion ; Erythroleukemia ; Erythropoietin ; Fundamental and applied biological sciences. 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The murine IW32 cell line is derived from an F-MuLV-induced erythroleukemia. An autocrine Epo production due to the rearrangement of one Epo allele has been previously described (Beru et al., 1989). However, the exact mechanism leading to the transcriptional activation of the abnormal Epo gene was unknown. In this study, we show that this deregulated expression results from a deletion within chromosome 5. The Epo gene in the abnormal allele is under the control of the G-protein β2 subunit gene promoter and the expressed mRNA results from the fusion of the non coding exon 1 of the G-protein β2 subunit gene to a truncated Epo exon 1 gene. This resulting abnormal cDNA allows the expression of a normal Epo protein.</description><subject>Alleles</subject><subject>Autocrine signalling</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</subject><subject>Chromosome 5</subject><subject>Chromosome deletion</subject><subject>Erythroleukemia</subject><subject>Erythropoietin</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene deletion</subject><subject>Gene expression</subject><subject>Gene rearrangement</subject><subject>Molecular and cellular biology</subject><subject>Proteins</subject><subject>Transcription activation</subject><issn>0950-9232</issn><issn>1476-5594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNo9Uc1O3DAQtqoidQtce7bUHtpDtuOxnZ8jQrAgrcQFxDFy0glkm9ip7Qh4pt54kD4TXrLqaTQz389oPsa-CFgLkOXPsFs7264FgpC5-sBWQhV5pnWlPrIVVBqyCiV-Yp9D2AFAUQGu2N-zxjo_moGTf4mP3k2up9hb_v1icj_4A1ni9Dx5CqF3lqdFfCQ-zr7fLxbKQPNvGnvDr-8l8paGIfBEmIcYeOfdyE1qjffGPtBINvKG4hPRIrXJJu8iJeF_r8jD3My2j4uvsb_eIemS98EJO-rMEOj0UI_Z3eXF7flVtr3ZXJ-fbbMWVaUyRFFWpUoFsezyXOpGV6glFgBaUaMagpawLEAVUrWqzFGDEbIEQ0ZgKY_Z10U3XfZnphDrnZu9TZY15kqgzAutE2q9oFrvQvDU1ZPvR-NfagH1PpA67OoUSH0IJBG-HWRNaM3QpX-0ffjPQlB67_4GRjyM7A</recordid><startdate>19971016</startdate><enddate>19971016</enddate><creator>CHRETIEN, S</creator><creator>DUPREZ, V</creator><creator>MAOUCHE, L</creator><creator>GISSELBRECHT, S</creator><creator>MAYEUX, P</creator><creator>LACOMBE, C</creator><general>Nature Publishing</general><general>Nature Publishing Group</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19971016</creationdate><title>Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein β2 subunit gene and the Epo gene</title><author>CHRETIEN, S ; DUPREZ, V ; MAOUCHE, L ; GISSELBRECHT, S ; MAYEUX, P ; LACOMBE, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2494-2218984221228f6635b59253270054eb4be0ce28704734c486250a1380aea1283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Alleles</topic><topic>Autocrine signalling</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes</topic><topic>Chromosome 5</topic><topic>Chromosome deletion</topic><topic>Erythroleukemia</topic><topic>Erythropoietin</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene deletion</topic><topic>Gene expression</topic><topic>Gene rearrangement</topic><topic>Molecular and cellular biology</topic><topic>Proteins</topic><topic>Transcription activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHRETIEN, S</creatorcontrib><creatorcontrib>DUPREZ, V</creatorcontrib><creatorcontrib>MAOUCHE, L</creatorcontrib><creatorcontrib>GISSELBRECHT, S</creatorcontrib><creatorcontrib>MAYEUX, P</creatorcontrib><creatorcontrib>LACOMBE, C</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Oncogene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHRETIEN, S</au><au>DUPREZ, V</au><au>MAOUCHE, L</au><au>GISSELBRECHT, S</au><au>MAYEUX, P</au><au>LACOMBE, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein β2 subunit gene and the Epo gene</atitle><jtitle>Oncogene</jtitle><date>1997-10-16</date><risdate>1997</risdate><volume>15</volume><issue>16</issue><spage>1995</spage><epage>1999</epage><pages>1995-1999</pages><issn>0950-9232</issn><eissn>1476-5594</eissn><abstract>Abnormal production of erythropoietin (Epo) has been described in several human and murine erythroleukemia. The murine IW32 cell line is derived from an F-MuLV-induced erythroleukemia. An autocrine Epo production due to the rearrangement of one Epo allele has been previously described (Beru et al., 1989). However, the exact mechanism leading to the transcriptional activation of the abnormal Epo gene was unknown. In this study, we show that this deregulated expression results from a deletion within chromosome 5. The Epo gene in the abnormal allele is under the control of the G-protein β2 subunit gene promoter and the expressed mRNA results from the fusion of the non coding exon 1 of the G-protein β2 subunit gene to a truncated Epo exon 1 gene. This resulting abnormal cDNA allows the expression of a normal Epo protein.</abstract><cop>Basingstoke</cop><pub>Nature Publishing</pub><doi>10.1038/sj.onc.1201364</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Oncogene, 1997-10, Vol.15 (16), p.1995-1999 |
| issn | 0950-9232 1476-5594 |
| language | eng |
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| source | Nature; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SpringerLink Journals - AutoHoldings |
| subjects | Alleles Autocrine signalling Biological and medical sciences Cell physiology Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes Chromosome 5 Chromosome deletion Erythroleukemia Erythropoietin Fundamental and applied biological sciences. Psychology Gene deletion Gene expression Gene rearrangement Molecular and cellular biology Proteins Transcription activation |
| title | Abnormal erythropoietin (Epo) gene expression in the murine erythroleukemia IW32 cells results from a rearrangement between the G-protein β2 subunit gene and the Epo gene |
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