Structural characterization of the carbohydrate and protein part of arabinogalactan protein from Basella alba stem and antiadhesive activity of polysaccharides from B. alba against Helicobacter pylori
Increasing drug resistance of Helicobacter pylori has highlighted the search for natural compounds with antiadhesive properties, interrupting the adhesion of H. pylori to stomach epithelia. Basella alba, a plant widely used in Asian traditional medicine, was investigated for its antiadhesive activit...
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| Veröffentlicht in: | Fitoterapia 2022-03, Vol.157 (NA), p.105132, Article 105132 |
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| creator | Nguyen, H.T. Herrmann, F. König, S. Goycoolea, F.M. Hensel, A. |
| description | Increasing drug resistance of Helicobacter pylori has highlighted the search for natural compounds with antiadhesive properties, interrupting the adhesion of H. pylori to stomach epithelia. Basella alba, a plant widely used in Asian traditional medicine, was investigated for its antiadhesive activity against H. pylori.
B. alba extract FE was prepared by aqueous extraction. Polysaccharides were isolated from FE by ethanol precipitation and arabinogalactan-protein (AGP) was isolated with Yariv reagent. Carbohydrate analyses was performed by standard methods and sequence analysis of the protein part of AGP by LC-MS. In vitro adhesion assay of fluorescent-labelled H. pylori J99 to human AGS cells was performed by flow cytometric analysis.
Raw polysaccharides (BA1) were isolated and 9% of BA1 were identified as AGP (53.1% neutral carbohydrates L-arabinose, D-galactose, rhamnose, 5.4% galacturonic acid, 41.5% protein). After deglycosylation of AGP, the protein part (two bands at 15 and 25 kDa in tricine SDS-PAGE) was shown to contain peptides like ribulose-bisphosphate-carboxylase-large-chain. Histological localization within the stem tissue of B. alba revealed that AGP was mainly located at the procambium ring. Functional assays indicated that neither FE nor BA1 had significant influence on viability of AGS cells or on H. pylori. FE inhibited the bacterial adhesion of H. pylori to AGS cells in a dose dependent manner. Best anti-adhesive effect of ~67% was observed with BA1 at 2 mg/mL.
The data obtained from this study characterize in part the mucilage and isolated polysaccharides of B. alba. As the polysaccharides interact with the bacterial adhesion, a potential uses a supplemental antiadhesive entity against the recurrence of H. pylori after eradication therapy may be discussed.
[Display omitted] |
| doi_str_mv | 10.1016/j.fitote.2022.105132 |
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B. alba extract FE was prepared by aqueous extraction. Polysaccharides were isolated from FE by ethanol precipitation and arabinogalactan-protein (AGP) was isolated with Yariv reagent. Carbohydrate analyses was performed by standard methods and sequence analysis of the protein part of AGP by LC-MS. In vitro adhesion assay of fluorescent-labelled H. pylori J99 to human AGS cells was performed by flow cytometric analysis.
Raw polysaccharides (BA1) were isolated and 9% of BA1 were identified as AGP (53.1% neutral carbohydrates L-arabinose, D-galactose, rhamnose, 5.4% galacturonic acid, 41.5% protein). After deglycosylation of AGP, the protein part (two bands at 15 and 25 kDa in tricine SDS-PAGE) was shown to contain peptides like ribulose-bisphosphate-carboxylase-large-chain. Histological localization within the stem tissue of B. alba revealed that AGP was mainly located at the procambium ring. Functional assays indicated that neither FE nor BA1 had significant influence on viability of AGS cells or on H. pylori. FE inhibited the bacterial adhesion of H. pylori to AGS cells in a dose dependent manner. Best anti-adhesive effect of ~67% was observed with BA1 at 2 mg/mL.
The data obtained from this study characterize in part the mucilage and isolated polysaccharides of B. alba. As the polysaccharides interact with the bacterial adhesion, a potential uses a supplemental antiadhesive entity against the recurrence of H. pylori after eradication therapy may be discussed.
[Display omitted]</description><identifier>ISSN: 0367-326X</identifier><identifier>EISSN: 1873-6971</identifier><identifier>DOI: 10.1016/j.fitote.2022.105132</identifier><identifier>PMID: 35074542</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adhesion ; Amino acid sequence ; Antiadhesion ; Arabinogalactan ; Arabinogalactan protein ; Arabinose ; Bacterial Adhesion - drug effects ; Basella alba ; Carbohydrate analysis ; Carbohydrates ; Caryophyllales - chemistry ; Chromatography, Thin Layer ; D-Galactose ; Deglycosylation ; Drug resistance ; Electrophoresis, Polyacrylamide Gel ; Ethanol ; Flow cytometry ; Fluorescence ; Galactans - chemistry ; Galactose ; Gel electrophoresis ; Helicobacter pylori ; Helicobacter pylori - drug effects ; Human performance ; Humans ; Immunodiffusion ; L-Arabinose ; Localization ; Medicinal plants ; Mucilage ; Peptides ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Stems - chemistry ; Polysaccharides ; Polysaccharides - isolation & purification ; Polysaccharides - pharmacology ; Proteins ; Reagents ; Rhamnose ; Ribulose bisphosphate carboxylase ; Ribulose-Bisphosphate Carboxylase - chemistry ; Saccharides ; Sequence analysis ; Sodium lauryl sulfate ; Stems ; Structural analysis ; Tandem Mass Spectrometry ; Tumor Cells, Cultured</subject><ispartof>Fitoterapia, 2022-03, Vol.157 (NA), p.105132, Article 105132</ispartof><rights>2022 Elsevier B.V.</rights><rights>Copyright © 2022 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Mar 2022</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-5d18a92660f1956cbd4d314675716bce4920c4847fe877b9bf5c41aa2bee4283</citedby><cites>FETCH-LOGICAL-c469t-5d18a92660f1956cbd4d314675716bce4920c4847fe877b9bf5c41aa2bee4283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0367326X22000107$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35074542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, H.T.</creatorcontrib><creatorcontrib>Herrmann, F.</creatorcontrib><creatorcontrib>König, S.</creatorcontrib><creatorcontrib>Goycoolea, F.M.</creatorcontrib><creatorcontrib>Hensel, A.</creatorcontrib><title>Structural characterization of the carbohydrate and protein part of arabinogalactan protein from Basella alba stem and antiadhesive activity of polysaccharides from B. alba against Helicobacter pylori</title><title>Fitoterapia</title><addtitle>Fitoterapia</addtitle><description>Increasing drug resistance of Helicobacter pylori has highlighted the search for natural compounds with antiadhesive properties, interrupting the adhesion of H. pylori to stomach epithelia. Basella alba, a plant widely used in Asian traditional medicine, was investigated for its antiadhesive activity against H. pylori.
B. alba extract FE was prepared by aqueous extraction. Polysaccharides were isolated from FE by ethanol precipitation and arabinogalactan-protein (AGP) was isolated with Yariv reagent. Carbohydrate analyses was performed by standard methods and sequence analysis of the protein part of AGP by LC-MS. In vitro adhesion assay of fluorescent-labelled H. pylori J99 to human AGS cells was performed by flow cytometric analysis.
Raw polysaccharides (BA1) were isolated and 9% of BA1 were identified as AGP (53.1% neutral carbohydrates L-arabinose, D-galactose, rhamnose, 5.4% galacturonic acid, 41.5% protein). After deglycosylation of AGP, the protein part (two bands at 15 and 25 kDa in tricine SDS-PAGE) was shown to contain peptides like ribulose-bisphosphate-carboxylase-large-chain. Histological localization within the stem tissue of B. alba revealed that AGP was mainly located at the procambium ring. Functional assays indicated that neither FE nor BA1 had significant influence on viability of AGS cells or on H. pylori. FE inhibited the bacterial adhesion of H. pylori to AGS cells in a dose dependent manner. Best anti-adhesive effect of ~67% was observed with BA1 at 2 mg/mL.
The data obtained from this study characterize in part the mucilage and isolated polysaccharides of B. alba. As the polysaccharides interact with the bacterial adhesion, a potential uses a supplemental antiadhesive entity against the recurrence of H. pylori after eradication therapy may be discussed.
[Display omitted]</description><subject>Adhesion</subject><subject>Amino acid sequence</subject><subject>Antiadhesion</subject><subject>Arabinogalactan</subject><subject>Arabinogalactan protein</subject><subject>Arabinose</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Basella alba</subject><subject>Carbohydrate analysis</subject><subject>Carbohydrates</subject><subject>Caryophyllales - chemistry</subject><subject>Chromatography, Thin Layer</subject><subject>D-Galactose</subject><subject>Deglycosylation</subject><subject>Drug resistance</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Ethanol</subject><subject>Flow cytometry</subject><subject>Fluorescence</subject><subject>Galactans - chemistry</subject><subject>Galactose</subject><subject>Gel electrophoresis</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - drug effects</subject><subject>Human performance</subject><subject>Humans</subject><subject>Immunodiffusion</subject><subject>L-Arabinose</subject><subject>Localization</subject><subject>Medicinal plants</subject><subject>Mucilage</subject><subject>Peptides</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Stems - chemistry</subject><subject>Polysaccharides</subject><subject>Polysaccharides - isolation & purification</subject><subject>Polysaccharides - pharmacology</subject><subject>Proteins</subject><subject>Reagents</subject><subject>Rhamnose</subject><subject>Ribulose bisphosphate carboxylase</subject><subject>Ribulose-Bisphosphate Carboxylase - chemistry</subject><subject>Saccharides</subject><subject>Sequence analysis</subject><subject>Sodium lauryl sulfate</subject><subject>Stems</subject><subject>Structural analysis</subject><subject>Tandem Mass Spectrometry</subject><subject>Tumor Cells, Cultured</subject><issn>0367-326X</issn><issn>1873-6971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFu1DAURSMEokPhDxCyxIZNBttx7GSDRCugSJVY0AU768V56XiUxMF2RgpfyGfhNEMXLFhZss-97z7fLHvN6J5RJt8f952NLuKeU87TVckK_iTbsUoVuawVe5rtaCFVXnD54yJ7EcKRUlYWgj3PLoqSKlEKvst-f49-NnH20BNzAA8more_IFo3EteReEBiwDfusLQeIhIYWzL5NNeOZAIfVyjJGju6e-iTHMbH9867gVxBwL4HAn0DJEQcHixgjBbaAwZ7Sp4m2pONy-o1uX4JYNYstsVw9thvcrgHO4ZIbrC3xjUPYcm09M7bl9mzDvqAr87nZXb3-dPd9U1---3L1-uPt7kRso552bIKai4l7VhdStO0oi2YkKpUTDYGRc2pEZVQHVZKNXXTlUYwAN4gCl4Vl9m7zTbt-HPGEPVgg1kXHNHNQXMlaapAcJrQt_-gRzf7MYXTXApaUUprmSixUca7EDx2evJ2AL9oRvVatD7qrWi9Fq23opPszdl8bgZsH0V_m03Ahw3A9Bkni14HY3E02FqPJurW2f9P-AN6Er_9</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Nguyen, H.T.</creator><creator>Herrmann, F.</creator><creator>König, S.</creator><creator>Goycoolea, F.M.</creator><creator>Hensel, A.</creator><general>Elsevier B.V</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20220301</creationdate><title>Structural characterization of the carbohydrate and protein part of arabinogalactan protein from Basella alba stem and antiadhesive activity of polysaccharides from B. alba against Helicobacter pylori</title><author>Nguyen, H.T. ; Herrmann, F. ; König, S. ; Goycoolea, F.M. ; Hensel, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-5d18a92660f1956cbd4d314675716bce4920c4847fe877b9bf5c41aa2bee4283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adhesion</topic><topic>Amino acid sequence</topic><topic>Antiadhesion</topic><topic>Arabinogalactan</topic><topic>Arabinogalactan protein</topic><topic>Arabinose</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Basella alba</topic><topic>Carbohydrate analysis</topic><topic>Carbohydrates</topic><topic>Caryophyllales - chemistry</topic><topic>Chromatography, Thin Layer</topic><topic>D-Galactose</topic><topic>Deglycosylation</topic><topic>Drug resistance</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Ethanol</topic><topic>Flow cytometry</topic><topic>Fluorescence</topic><topic>Galactans - chemistry</topic><topic>Galactose</topic><topic>Gel electrophoresis</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - drug effects</topic><topic>Human performance</topic><topic>Humans</topic><topic>Immunodiffusion</topic><topic>L-Arabinose</topic><topic>Localization</topic><topic>Medicinal plants</topic><topic>Mucilage</topic><topic>Peptides</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Stems - chemistry</topic><topic>Polysaccharides</topic><topic>Polysaccharides - isolation & purification</topic><topic>Polysaccharides - pharmacology</topic><topic>Proteins</topic><topic>Reagents</topic><topic>Rhamnose</topic><topic>Ribulose bisphosphate carboxylase</topic><topic>Ribulose-Bisphosphate Carboxylase - chemistry</topic><topic>Saccharides</topic><topic>Sequence analysis</topic><topic>Sodium lauryl sulfate</topic><topic>Stems</topic><topic>Structural analysis</topic><topic>Tandem Mass Spectrometry</topic><topic>Tumor Cells, Cultured</topic><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, H.T.</creatorcontrib><creatorcontrib>Herrmann, F.</creatorcontrib><creatorcontrib>König, S.</creatorcontrib><creatorcontrib>Goycoolea, F.M.</creatorcontrib><creatorcontrib>Hensel, A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Fitoterapia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, H.T.</au><au>Herrmann, F.</au><au>König, S.</au><au>Goycoolea, F.M.</au><au>Hensel, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural characterization of the carbohydrate and protein part of arabinogalactan protein from Basella alba stem and antiadhesive activity of polysaccharides from B. alba against Helicobacter pylori</atitle><jtitle>Fitoterapia</jtitle><addtitle>Fitoterapia</addtitle><date>2022-03-01</date><risdate>2022</risdate><volume>157</volume><issue>NA</issue><spage>105132</spage><pages>105132-</pages><artnum>105132</artnum><issn>0367-326X</issn><eissn>1873-6971</eissn><abstract>Increasing drug resistance of Helicobacter pylori has highlighted the search for natural compounds with antiadhesive properties, interrupting the adhesion of H. pylori to stomach epithelia. Basella alba, a plant widely used in Asian traditional medicine, was investigated for its antiadhesive activity against H. pylori.
B. alba extract FE was prepared by aqueous extraction. Polysaccharides were isolated from FE by ethanol precipitation and arabinogalactan-protein (AGP) was isolated with Yariv reagent. Carbohydrate analyses was performed by standard methods and sequence analysis of the protein part of AGP by LC-MS. In vitro adhesion assay of fluorescent-labelled H. pylori J99 to human AGS cells was performed by flow cytometric analysis.
Raw polysaccharides (BA1) were isolated and 9% of BA1 were identified as AGP (53.1% neutral carbohydrates L-arabinose, D-galactose, rhamnose, 5.4% galacturonic acid, 41.5% protein). After deglycosylation of AGP, the protein part (two bands at 15 and 25 kDa in tricine SDS-PAGE) was shown to contain peptides like ribulose-bisphosphate-carboxylase-large-chain. Histological localization within the stem tissue of B. alba revealed that AGP was mainly located at the procambium ring. Functional assays indicated that neither FE nor BA1 had significant influence on viability of AGS cells or on H. pylori. FE inhibited the bacterial adhesion of H. pylori to AGS cells in a dose dependent manner. Best anti-adhesive effect of ~67% was observed with BA1 at 2 mg/mL.
The data obtained from this study characterize in part the mucilage and isolated polysaccharides of B. alba. As the polysaccharides interact with the bacterial adhesion, a potential uses a supplemental antiadhesive entity against the recurrence of H. pylori after eradication therapy may be discussed.
[Display omitted]</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>35074542</pmid><doi>10.1016/j.fitote.2022.105132</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesion Amino acid sequence Antiadhesion Arabinogalactan Arabinogalactan protein Arabinose Bacterial Adhesion - drug effects Basella alba Carbohydrate analysis Carbohydrates Caryophyllales - chemistry Chromatography, Thin Layer D-Galactose Deglycosylation Drug resistance Electrophoresis, Polyacrylamide Gel Ethanol Flow cytometry Fluorescence Galactans - chemistry Galactose Gel electrophoresis Helicobacter pylori Helicobacter pylori - drug effects Human performance Humans Immunodiffusion L-Arabinose Localization Medicinal plants Mucilage Peptides Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Stems - chemistry Polysaccharides Polysaccharides - isolation & purification Polysaccharides - pharmacology Proteins Reagents Rhamnose Ribulose bisphosphate carboxylase Ribulose-Bisphosphate Carboxylase - chemistry Saccharides Sequence analysis Sodium lauryl sulfate Stems Structural analysis Tandem Mass Spectrometry Tumor Cells, Cultured |
| title | Structural characterization of the carbohydrate and protein part of arabinogalactan protein from Basella alba stem and antiadhesive activity of polysaccharides from B. alba against Helicobacter pylori |
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