Protective immunity against infectious spleen and kidney necrosis virus induced by mannose modified subunit vaccine with carbon nanotubes in mandarin fish
Due to its wide host range and frequently causing significant mortality, infectious spleen and kidney necrosis virus (ISKNV) has caused huge economic losses on mandarin fish in China. There are no commercial drugs to cure ISKNV, and vaccines are regarded as one of the most effective measures to cont...
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| Veröffentlicht in: | Aquaculture research 2022-04, Vol.53 (6), p.2175-2184 |
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| description | Due to its wide host range and frequently causing significant mortality, infectious spleen and kidney necrosis virus (ISKNV) has caused huge economic losses on mandarin fish in China. There are no commercial drugs to cure ISKNV, and vaccines are regarded as one of the most effective measures to control virus infection. Because of the weak immunogenicity of antigen protein, subunit vaccines may require multiple booster immunizations, adjuvants or carriers to ensure strong and long‐term protective immunity. In this work, based on MCP (main capsid protein) subunit vaccine, we used single‐walled carbon nanotubes (SWCNTs) as carrier and mannose (M) as targeting factors to construct SWCNTs‐M‐MCP vaccine. After injection immunization, rapid and strong antibody titres were detected in SWCNTs‐M‐MCP groups which were significantly higher than that of MCP groups and SWCNTs‐MCP groups at the same immune doses. The antibody titre of the highest immune group (5 μg SWCNTs‐M‐MCP) reached more than 2.5 times that of the control group at 21 days postvaccination. Similar results were found in enzyme activities and immune‐related genes expression. All enzyme activities and immune‐related genes expression of 5 μg SWCNTs‐M‐MCP group at 21 days postvaccination were significantly higher than those of all dosages MCP groups and SWCNTs‐MCP groups. This study showed that SWCNTs and mannose can significantly improve the immune responses of vaccines and may provide an effective strategy against ISKNV. |
| doi_str_mv | 10.1111/are.15736 |
| format | Article |
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There are no commercial drugs to cure ISKNV, and vaccines are regarded as one of the most effective measures to control virus infection. Because of the weak immunogenicity of antigen protein, subunit vaccines may require multiple booster immunizations, adjuvants or carriers to ensure strong and long‐term protective immunity. In this work, based on MCP (main capsid protein) subunit vaccine, we used single‐walled carbon nanotubes (SWCNTs) as carrier and mannose (M) as targeting factors to construct SWCNTs‐M‐MCP vaccine. After injection immunization, rapid and strong antibody titres were detected in SWCNTs‐M‐MCP groups which were significantly higher than that of MCP groups and SWCNTs‐MCP groups at the same immune doses. The antibody titre of the highest immune group (5 μg SWCNTs‐M‐MCP) reached more than 2.5 times that of the control group at 21 days postvaccination. Similar results were found in enzyme activities and immune‐related genes expression. All enzyme activities and immune‐related genes expression of 5 μg SWCNTs‐M‐MCP group at 21 days postvaccination were significantly higher than those of all dosages MCP groups and SWCNTs‐MCP groups. This study showed that SWCNTs and mannose can significantly improve the immune responses of vaccines and may provide an effective strategy against ISKNV.</description><identifier>ISSN: 1355-557X</identifier><identifier>EISSN: 1365-2109</identifier><identifier>DOI: 10.1111/are.15736</identifier><language>eng</language><publisher>Oxford: Hindawi Limited</publisher><subject>Adjuvants ; Antibodies ; Antigens ; Capsid protein ; Carbon ; Disease control ; Economic impact ; Economics ; Enzymatic activity ; Enzyme activity ; Enzymes ; Fish ; Fish diseases ; Gene expression ; Genes ; Host range ; Immune response ; Immunity ; Immunization ; Immunogenicity ; Immunosuppressive agents ; infectious spleen and kidney necrosis virus ; Kidneys ; mandarin fish ; Mannose ; Nanotechnology ; Nanotubes ; Necrosis ; Proteins ; Single wall carbon nanotubes ; Spleen ; subunit vaccine ; SWCNTs ; Vaccines ; Viruses</subject><ispartof>Aquaculture research, 2022-04, Vol.53 (6), p.2175-2184</ispartof><rights>2022 John Wiley & Sons Ltd</rights><rights>Copyright © 2022 John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2976-540b0b94afe7d0c26057b133eb5f136d58252f1c6f6d8ccc663999e1454bc7193</citedby><cites>FETCH-LOGICAL-c2976-540b0b94afe7d0c26057b133eb5f136d58252f1c6f6d8ccc663999e1454bc7193</cites><orcidid>0000-0001-5702-524X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fare.15736$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fare.15736$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Zhao, Zhao</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Chen, Guo</creatorcontrib><creatorcontrib>Zhang, Chen</creatorcontrib><creatorcontrib>Wang, Gao‐Xue</creatorcontrib><creatorcontrib>Zhu, Bin</creatorcontrib><title>Protective immunity against infectious spleen and kidney necrosis virus induced by mannose modified subunit vaccine with carbon nanotubes in mandarin fish</title><title>Aquaculture research</title><description>Due to its wide host range and frequently causing significant mortality, infectious spleen and kidney necrosis virus (ISKNV) has caused huge economic losses on mandarin fish in China. There are no commercial drugs to cure ISKNV, and vaccines are regarded as one of the most effective measures to control virus infection. Because of the weak immunogenicity of antigen protein, subunit vaccines may require multiple booster immunizations, adjuvants or carriers to ensure strong and long‐term protective immunity. In this work, based on MCP (main capsid protein) subunit vaccine, we used single‐walled carbon nanotubes (SWCNTs) as carrier and mannose (M) as targeting factors to construct SWCNTs‐M‐MCP vaccine. After injection immunization, rapid and strong antibody titres were detected in SWCNTs‐M‐MCP groups which were significantly higher than that of MCP groups and SWCNTs‐MCP groups at the same immune doses. The antibody titre of the highest immune group (5 μg SWCNTs‐M‐MCP) reached more than 2.5 times that of the control group at 21 days postvaccination. Similar results were found in enzyme activities and immune‐related genes expression. All enzyme activities and immune‐related genes expression of 5 μg SWCNTs‐M‐MCP group at 21 days postvaccination were significantly higher than those of all dosages MCP groups and SWCNTs‐MCP groups. This study showed that SWCNTs and mannose can significantly improve the immune responses of vaccines and may provide an effective strategy against ISKNV.</description><subject>Adjuvants</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Capsid protein</subject><subject>Carbon</subject><subject>Disease control</subject><subject>Economic impact</subject><subject>Economics</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Fish</subject><subject>Fish diseases</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Host range</subject><subject>Immune response</subject><subject>Immunity</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Immunosuppressive agents</subject><subject>infectious spleen and kidney necrosis virus</subject><subject>Kidneys</subject><subject>mandarin fish</subject><subject>Mannose</subject><subject>Nanotechnology</subject><subject>Nanotubes</subject><subject>Necrosis</subject><subject>Proteins</subject><subject>Single wall carbon nanotubes</subject><subject>Spleen</subject><subject>subunit vaccine</subject><subject>SWCNTs</subject><subject>Vaccines</subject><subject>Viruses</subject><issn>1355-557X</issn><issn>1365-2109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kE1PwzAMhisEEmNw4B9E4sShW9I27XKcpvEhTQIhkLhV-XBYxpqMpN3Uv8KvJWVc8cWW_fi1_CbJNcETEmPKPUwIrfLyJBmRvKRpRjA7HWpKU0qr9_PkIoQNxqTAORkl38_etSBbswdkmqazpu0R_-DGhhYZq4eR6wIKuy2ARdwq9GmUhR5ZkN4FE9De-AgYqzoJCokeNdxaFwA1ThltYi90YhBGey6lsYAOpl0jyb1wFlluXdsJGBSGTcV9LLQJ68vkTPNtgKu_PE7e7pavi4d09XT_uJivUpmxqkxpgQUWrOAaKoVlVmJaCZLnIKiODig6y2imiSx1qWZSyrLMGWNACloIWRGWj5Obo-7Ou68OQltvXOdtPFlnkSUsy1kRqdsjNXwdPOh6503DfV8TXA_W19H6-tf6yE6P7MFsof8frOcvy-PGD2_YiRA</recordid><startdate>202204</startdate><enddate>202204</enddate><creator>Zhao, Zhao</creator><creator>Li, Yang</creator><creator>Chen, Guo</creator><creator>Zhang, Chen</creator><creator>Wang, Gao‐Xue</creator><creator>Zhu, Bin</creator><general>Hindawi Limited</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0001-5702-524X</orcidid></search><sort><creationdate>202204</creationdate><title>Protective immunity against infectious spleen and kidney necrosis virus induced by mannose modified subunit vaccine with carbon nanotubes in mandarin fish</title><author>Zhao, Zhao ; Li, Yang ; Chen, Guo ; Zhang, Chen ; Wang, Gao‐Xue ; Zhu, Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2976-540b0b94afe7d0c26057b133eb5f136d58252f1c6f6d8ccc663999e1454bc7193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Adjuvants</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Capsid protein</topic><topic>Carbon</topic><topic>Disease control</topic><topic>Economic impact</topic><topic>Economics</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Fish</topic><topic>Fish diseases</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Host range</topic><topic>Immune response</topic><topic>Immunity</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Immunosuppressive agents</topic><topic>infectious spleen and kidney necrosis virus</topic><topic>Kidneys</topic><topic>mandarin fish</topic><topic>Mannose</topic><topic>Nanotechnology</topic><topic>Nanotubes</topic><topic>Necrosis</topic><topic>Proteins</topic><topic>Single wall carbon nanotubes</topic><topic>Spleen</topic><topic>subunit vaccine</topic><topic>SWCNTs</topic><topic>Vaccines</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Zhao</creatorcontrib><creatorcontrib>Li, Yang</creatorcontrib><creatorcontrib>Chen, Guo</creatorcontrib><creatorcontrib>Zhang, Chen</creatorcontrib><creatorcontrib>Wang, Gao‐Xue</creatorcontrib><creatorcontrib>Zhu, Bin</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Aquaculture research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Zhao</au><au>Li, Yang</au><au>Chen, Guo</au><au>Zhang, Chen</au><au>Wang, Gao‐Xue</au><au>Zhu, Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective immunity against infectious spleen and kidney necrosis virus induced by mannose modified subunit vaccine with carbon nanotubes in mandarin fish</atitle><jtitle>Aquaculture research</jtitle><date>2022-04</date><risdate>2022</risdate><volume>53</volume><issue>6</issue><spage>2175</spage><epage>2184</epage><pages>2175-2184</pages><issn>1355-557X</issn><eissn>1365-2109</eissn><abstract>Due to its wide host range and frequently causing significant mortality, infectious spleen and kidney necrosis virus (ISKNV) has caused huge economic losses on mandarin fish in China. There are no commercial drugs to cure ISKNV, and vaccines are regarded as one of the most effective measures to control virus infection. Because of the weak immunogenicity of antigen protein, subunit vaccines may require multiple booster immunizations, adjuvants or carriers to ensure strong and long‐term protective immunity. In this work, based on MCP (main capsid protein) subunit vaccine, we used single‐walled carbon nanotubes (SWCNTs) as carrier and mannose (M) as targeting factors to construct SWCNTs‐M‐MCP vaccine. After injection immunization, rapid and strong antibody titres were detected in SWCNTs‐M‐MCP groups which were significantly higher than that of MCP groups and SWCNTs‐MCP groups at the same immune doses. The antibody titre of the highest immune group (5 μg SWCNTs‐M‐MCP) reached more than 2.5 times that of the control group at 21 days postvaccination. Similar results were found in enzyme activities and immune‐related genes expression. All enzyme activities and immune‐related genes expression of 5 μg SWCNTs‐M‐MCP group at 21 days postvaccination were significantly higher than those of all dosages MCP groups and SWCNTs‐MCP groups. This study showed that SWCNTs and mannose can significantly improve the immune responses of vaccines and may provide an effective strategy against ISKNV.</abstract><cop>Oxford</cop><pub>Hindawi Limited</pub><doi>10.1111/are.15736</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-5702-524X</orcidid></addata></record> |
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| subjects | Adjuvants Antibodies Antigens Capsid protein Carbon Disease control Economic impact Economics Enzymatic activity Enzyme activity Enzymes Fish Fish diseases Gene expression Genes Host range Immune response Immunity Immunization Immunogenicity Immunosuppressive agents infectious spleen and kidney necrosis virus Kidneys mandarin fish Mannose Nanotechnology Nanotubes Necrosis Proteins Single wall carbon nanotubes Spleen subunit vaccine SWCNTs Vaccines Viruses |
| title | Protective immunity against infectious spleen and kidney necrosis virus induced by mannose modified subunit vaccine with carbon nanotubes in mandarin fish |
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