A disposable ratiometric electrochemical aptasensor with exonuclease I-powered target recycling amplification for highly sensitive detection of aflatoxin B1
A disposable ratiometric electrochemical aptasensor has been constructed for highly sensitive detection of aflatoxin B1 (AFB1) with exonuclease I (Exo I)-powered cascade amplification. Specifically, the in-situ growth of Au nanoflowers (AuNFs) on indium tin oxide (ITO) surface was used to anchor met...
Gespeichert in:
| Veröffentlicht in: | Sensors and actuators. B, Chemical Chemical, 2022-03, Vol.355, p.131238, Article 131238 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | 131238 |
| container_title | Sensors and actuators. B, Chemical |
| container_volume | 355 |
| creator | Cui, Haining An, Keqi Wang, Chengquan Chen, Yangfang Jia, Suli Qian, Jing Hao, Nan Wei, Jie Wang, Kun |
| description | A disposable ratiometric electrochemical aptasensor has been constructed for highly sensitive detection of aflatoxin B1 (AFB1) with exonuclease I (Exo I)-powered cascade amplification. Specifically, the in-situ growth of Au nanoflowers (AuNFs) on indium tin oxide (ITO) surface was used to anchor methylene blue (MB) labeled the complementary DNA of aptamer. Ferrocene (Fc) labeled aptamer was then hybrid with cDNA(MB) to produce a duplex structure. Due to the rigid structure of the duplex structure, Fc molecules were close to the electrode surface while MB molecules were far away, which respectively produced a faded external response signal and an enhanced internal reference signal. When AFB1 was present in sample, the aptamers specifically bond with the targets and then detached from electrode surface, which resulted a signal-off for Fc indicators. Meanwhile, more MB molecules were close to the electrode surface owing to an increasing target concentration, which caused a signal-on for MB indicators. On this basis, the ratiometric electrochemical detection of AFB1 was achieved employing the peak current intensity ratio (IMB/IFc) as signal transducer. Moreover, Exo I was introduced to digest the detached aptamer, and the released AFB1 entered the next round of circulation which formed the target recycling and realized the signal amplification. This aptasensor exhibited a wide detection range (0.1–1000 pg mL−1) and a low detection limit (0.032 pg mL−1). We anticipate this method may find good utility in food quality control and further facilitate the development of aptasensor for other targets.
[Display omitted]
•A disposable ratiometric electrochemical aptasensor was developed for AFB1 detection.•The aptasensor has high sensitivity owing to the Exo I-powered cascade amplification.•In-situ growth of AuNFs on ITO offers a firm anchoring sites for thiolated aptamer.•A general strategy in advancing ratiometric electrochemical aptasensor was proposed. |
| doi_str_mv | 10.1016/j.snb.2021.131238 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2638771174</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0925400521018062</els_id><sourcerecordid>2638771174</sourcerecordid><originalsourceid>FETCH-LOGICAL-c325t-a73f494b5d6ed0f39d6fa4ad54559d4a3c0a6c7fc9a74bd917db80bb7c96a4ca3</originalsourceid><addsrcrecordid>eNp9kcFOGzEQhq0KJAL0AbhZ4rypvfaus-qJokKRkHopZ2vWHieOnPViO0DehYeto_Tc00gz___NaH5Cbjhbcsb7b9tlnsZly1q-5IK3YvWFLPhKiUYwpc7Igg1t10jGugtymfOWMSZFzxbk845an-eYYQxIExQfd1iSNxQDmpKi2eDOGwgU5gIZpxwTffdlQ_EjTnsTsDbpUzPHd0xoaYG0xkITmoMJflpT2M3Bu0qo5Im66t749SYc6JHli39DarHUVcdxdBRcgBI__ER_8Gty7iBk_PqvXpGXh59_7n81z78fn-7vnhsj2q40oISTgxw726NlTgy2dyDBdrLrBitBGAa9Uc4MoORoB67suGLjqMzQgzQgrsjtiTun-LrHXPQ27tNUV-q2FyulOFeyqvhJZVLMOaHTc_I7SAfNmT6GoLe6hqCPIehTCNXz_eTBev6bx6Sz8TgZtL6-qGgb_X_cfwE-zpR0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2638771174</pqid></control><display><type>article</type><title>A disposable ratiometric electrochemical aptasensor with exonuclease I-powered target recycling amplification for highly sensitive detection of aflatoxin B1</title><source>Elsevier ScienceDirect Journals</source><creator>Cui, Haining ; An, Keqi ; Wang, Chengquan ; Chen, Yangfang ; Jia, Suli ; Qian, Jing ; Hao, Nan ; Wei, Jie ; Wang, Kun</creator><creatorcontrib>Cui, Haining ; An, Keqi ; Wang, Chengquan ; Chen, Yangfang ; Jia, Suli ; Qian, Jing ; Hao, Nan ; Wei, Jie ; Wang, Kun</creatorcontrib><description>A disposable ratiometric electrochemical aptasensor has been constructed for highly sensitive detection of aflatoxin B1 (AFB1) with exonuclease I (Exo I)-powered cascade amplification. Specifically, the in-situ growth of Au nanoflowers (AuNFs) on indium tin oxide (ITO) surface was used to anchor methylene blue (MB) labeled the complementary DNA of aptamer. Ferrocene (Fc) labeled aptamer was then hybrid with cDNA(MB) to produce a duplex structure. Due to the rigid structure of the duplex structure, Fc molecules were close to the electrode surface while MB molecules were far away, which respectively produced a faded external response signal and an enhanced internal reference signal. When AFB1 was present in sample, the aptamers specifically bond with the targets and then detached from electrode surface, which resulted a signal-off for Fc indicators. Meanwhile, more MB molecules were close to the electrode surface owing to an increasing target concentration, which caused a signal-on for MB indicators. On this basis, the ratiometric electrochemical detection of AFB1 was achieved employing the peak current intensity ratio (IMB/IFc) as signal transducer. Moreover, Exo I was introduced to digest the detached aptamer, and the released AFB1 entered the next round of circulation which formed the target recycling and realized the signal amplification. This aptasensor exhibited a wide detection range (0.1–1000 pg mL−1) and a low detection limit (0.032 pg mL−1). We anticipate this method may find good utility in food quality control and further facilitate the development of aptasensor for other targets.
[Display omitted]
•A disposable ratiometric electrochemical aptasensor was developed for AFB1 detection.•The aptasensor has high sensitivity owing to the Exo I-powered cascade amplification.•In-situ growth of AuNFs on ITO offers a firm anchoring sites for thiolated aptamer.•A general strategy in advancing ratiometric electrochemical aptasensor was proposed.</description><identifier>ISSN: 0925-4005</identifier><identifier>EISSN: 1873-3077</identifier><identifier>DOI: 10.1016/j.snb.2021.131238</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Aflatoxin B1 ; Aflatoxins ; Amplification ; Aptamer ; Electrochemical analysis ; Electrodes ; Exonuclease I ; Indicators ; Indium tin oxides ; Methylene blue ; Molecular structure ; Quality control ; Ratiometric electrochemical aptasensor ; Recycling ; Reference signals ; Rigid structures ; Signal amplification</subject><ispartof>Sensors and actuators. B, Chemical, 2022-03, Vol.355, p.131238, Article 131238</ispartof><rights>2021 Elsevier B.V.</rights><rights>Copyright Elsevier Science Ltd. Mar 15, 2022</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c325t-a73f494b5d6ed0f39d6fa4ad54559d4a3c0a6c7fc9a74bd917db80bb7c96a4ca3</citedby><cites>FETCH-LOGICAL-c325t-a73f494b5d6ed0f39d6fa4ad54559d4a3c0a6c7fc9a74bd917db80bb7c96a4ca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0925400521018062$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Cui, Haining</creatorcontrib><creatorcontrib>An, Keqi</creatorcontrib><creatorcontrib>Wang, Chengquan</creatorcontrib><creatorcontrib>Chen, Yangfang</creatorcontrib><creatorcontrib>Jia, Suli</creatorcontrib><creatorcontrib>Qian, Jing</creatorcontrib><creatorcontrib>Hao, Nan</creatorcontrib><creatorcontrib>Wei, Jie</creatorcontrib><creatorcontrib>Wang, Kun</creatorcontrib><title>A disposable ratiometric electrochemical aptasensor with exonuclease I-powered target recycling amplification for highly sensitive detection of aflatoxin B1</title><title>Sensors and actuators. B, Chemical</title><description>A disposable ratiometric electrochemical aptasensor has been constructed for highly sensitive detection of aflatoxin B1 (AFB1) with exonuclease I (Exo I)-powered cascade amplification. Specifically, the in-situ growth of Au nanoflowers (AuNFs) on indium tin oxide (ITO) surface was used to anchor methylene blue (MB) labeled the complementary DNA of aptamer. Ferrocene (Fc) labeled aptamer was then hybrid with cDNA(MB) to produce a duplex structure. Due to the rigid structure of the duplex structure, Fc molecules were close to the electrode surface while MB molecules were far away, which respectively produced a faded external response signal and an enhanced internal reference signal. When AFB1 was present in sample, the aptamers specifically bond with the targets and then detached from electrode surface, which resulted a signal-off for Fc indicators. Meanwhile, more MB molecules were close to the electrode surface owing to an increasing target concentration, which caused a signal-on for MB indicators. On this basis, the ratiometric electrochemical detection of AFB1 was achieved employing the peak current intensity ratio (IMB/IFc) as signal transducer. Moreover, Exo I was introduced to digest the detached aptamer, and the released AFB1 entered the next round of circulation which formed the target recycling and realized the signal amplification. This aptasensor exhibited a wide detection range (0.1–1000 pg mL−1) and a low detection limit (0.032 pg mL−1). We anticipate this method may find good utility in food quality control and further facilitate the development of aptasensor for other targets.
[Display omitted]
•A disposable ratiometric electrochemical aptasensor was developed for AFB1 detection.•The aptasensor has high sensitivity owing to the Exo I-powered cascade amplification.•In-situ growth of AuNFs on ITO offers a firm anchoring sites for thiolated aptamer.•A general strategy in advancing ratiometric electrochemical aptasensor was proposed.</description><subject>Aflatoxin B1</subject><subject>Aflatoxins</subject><subject>Amplification</subject><subject>Aptamer</subject><subject>Electrochemical analysis</subject><subject>Electrodes</subject><subject>Exonuclease I</subject><subject>Indicators</subject><subject>Indium tin oxides</subject><subject>Methylene blue</subject><subject>Molecular structure</subject><subject>Quality control</subject><subject>Ratiometric electrochemical aptasensor</subject><subject>Recycling</subject><subject>Reference signals</subject><subject>Rigid structures</subject><subject>Signal amplification</subject><issn>0925-4005</issn><issn>1873-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp9kcFOGzEQhq0KJAL0AbhZ4rypvfaus-qJokKRkHopZ2vWHieOnPViO0DehYeto_Tc00gz___NaH5Cbjhbcsb7b9tlnsZly1q-5IK3YvWFLPhKiUYwpc7Igg1t10jGugtymfOWMSZFzxbk845an-eYYQxIExQfd1iSNxQDmpKi2eDOGwgU5gIZpxwTffdlQ_EjTnsTsDbpUzPHd0xoaYG0xkITmoMJflpT2M3Bu0qo5Im66t749SYc6JHli39DarHUVcdxdBRcgBI__ER_8Gty7iBk_PqvXpGXh59_7n81z78fn-7vnhsj2q40oISTgxw726NlTgy2dyDBdrLrBitBGAa9Uc4MoORoB67suGLjqMzQgzQgrsjtiTun-LrHXPQ27tNUV-q2FyulOFeyqvhJZVLMOaHTc_I7SAfNmT6GoLe6hqCPIehTCNXz_eTBev6bx6Sz8TgZtL6-qGgb_X_cfwE-zpR0</recordid><startdate>20220315</startdate><enddate>20220315</enddate><creator>Cui, Haining</creator><creator>An, Keqi</creator><creator>Wang, Chengquan</creator><creator>Chen, Yangfang</creator><creator>Jia, Suli</creator><creator>Qian, Jing</creator><creator>Hao, Nan</creator><creator>Wei, Jie</creator><creator>Wang, Kun</creator><general>Elsevier B.V</general><general>Elsevier Science Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20220315</creationdate><title>A disposable ratiometric electrochemical aptasensor with exonuclease I-powered target recycling amplification for highly sensitive detection of aflatoxin B1</title><author>Cui, Haining ; An, Keqi ; Wang, Chengquan ; Chen, Yangfang ; Jia, Suli ; Qian, Jing ; Hao, Nan ; Wei, Jie ; Wang, Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-a73f494b5d6ed0f39d6fa4ad54559d4a3c0a6c7fc9a74bd917db80bb7c96a4ca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aflatoxin B1</topic><topic>Aflatoxins</topic><topic>Amplification</topic><topic>Aptamer</topic><topic>Electrochemical analysis</topic><topic>Electrodes</topic><topic>Exonuclease I</topic><topic>Indicators</topic><topic>Indium tin oxides</topic><topic>Methylene blue</topic><topic>Molecular structure</topic><topic>Quality control</topic><topic>Ratiometric electrochemical aptasensor</topic><topic>Recycling</topic><topic>Reference signals</topic><topic>Rigid structures</topic><topic>Signal amplification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cui, Haining</creatorcontrib><creatorcontrib>An, Keqi</creatorcontrib><creatorcontrib>Wang, Chengquan</creatorcontrib><creatorcontrib>Chen, Yangfang</creatorcontrib><creatorcontrib>Jia, Suli</creatorcontrib><creatorcontrib>Qian, Jing</creatorcontrib><creatorcontrib>Hao, Nan</creatorcontrib><creatorcontrib>Wei, Jie</creatorcontrib><creatorcontrib>Wang, Kun</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Sensors and actuators. B, Chemical</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cui, Haining</au><au>An, Keqi</au><au>Wang, Chengquan</au><au>Chen, Yangfang</au><au>Jia, Suli</au><au>Qian, Jing</au><au>Hao, Nan</au><au>Wei, Jie</au><au>Wang, Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A disposable ratiometric electrochemical aptasensor with exonuclease I-powered target recycling amplification for highly sensitive detection of aflatoxin B1</atitle><jtitle>Sensors and actuators. B, Chemical</jtitle><date>2022-03-15</date><risdate>2022</risdate><volume>355</volume><spage>131238</spage><pages>131238-</pages><artnum>131238</artnum><issn>0925-4005</issn><eissn>1873-3077</eissn><abstract>A disposable ratiometric electrochemical aptasensor has been constructed for highly sensitive detection of aflatoxin B1 (AFB1) with exonuclease I (Exo I)-powered cascade amplification. Specifically, the in-situ growth of Au nanoflowers (AuNFs) on indium tin oxide (ITO) surface was used to anchor methylene blue (MB) labeled the complementary DNA of aptamer. Ferrocene (Fc) labeled aptamer was then hybrid with cDNA(MB) to produce a duplex structure. Due to the rigid structure of the duplex structure, Fc molecules were close to the electrode surface while MB molecules were far away, which respectively produced a faded external response signal and an enhanced internal reference signal. When AFB1 was present in sample, the aptamers specifically bond with the targets and then detached from electrode surface, which resulted a signal-off for Fc indicators. Meanwhile, more MB molecules were close to the electrode surface owing to an increasing target concentration, which caused a signal-on for MB indicators. On this basis, the ratiometric electrochemical detection of AFB1 was achieved employing the peak current intensity ratio (IMB/IFc) as signal transducer. Moreover, Exo I was introduced to digest the detached aptamer, and the released AFB1 entered the next round of circulation which formed the target recycling and realized the signal amplification. This aptasensor exhibited a wide detection range (0.1–1000 pg mL−1) and a low detection limit (0.032 pg mL−1). We anticipate this method may find good utility in food quality control and further facilitate the development of aptasensor for other targets.
[Display omitted]
•A disposable ratiometric electrochemical aptasensor was developed for AFB1 detection.•The aptasensor has high sensitivity owing to the Exo I-powered cascade amplification.•In-situ growth of AuNFs on ITO offers a firm anchoring sites for thiolated aptamer.•A general strategy in advancing ratiometric electrochemical aptasensor was proposed.</abstract><cop>Lausanne</cop><pub>Elsevier B.V</pub><doi>10.1016/j.snb.2021.131238</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0925-4005 |
| ispartof | Sensors and actuators. B, Chemical, 2022-03, Vol.355, p.131238, Article 131238 |
| issn | 0925-4005 1873-3077 |
| language | eng |
| recordid | cdi_proquest_journals_2638771174 |
| source | Elsevier ScienceDirect Journals |
| subjects | Aflatoxin B1 Aflatoxins Amplification Aptamer Electrochemical analysis Electrodes Exonuclease I Indicators Indium tin oxides Methylene blue Molecular structure Quality control Ratiometric electrochemical aptasensor Recycling Reference signals Rigid structures Signal amplification |
| title | A disposable ratiometric electrochemical aptasensor with exonuclease I-powered target recycling amplification for highly sensitive detection of aflatoxin B1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T09%3A31%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20disposable%20ratiometric%20electrochemical%20aptasensor%20with%20exonuclease%20I-powered%20target%20recycling%20amplification%20for%20highly%20sensitive%20detection%20of%20aflatoxin%20B1&rft.jtitle=Sensors%20and%20actuators.%20B,%20Chemical&rft.au=Cui,%20Haining&rft.date=2022-03-15&rft.volume=355&rft.spage=131238&rft.pages=131238-&rft.artnum=131238&rft.issn=0925-4005&rft.eissn=1873-3077&rft_id=info:doi/10.1016/j.snb.2021.131238&rft_dat=%3Cproquest_cross%3E2638771174%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2638771174&rft_id=info:pmid/&rft_els_id=S0925400521018062&rfr_iscdi=true |