Isotropic super-resolution light-sheet microscopy of dynamic intracellular structures at subsecond timescales

Long-term visualization of the dynamic interactions between intracellular structures throughout the three-dimensional space of whole live cells is essential to better understand their functions, but this task remains challenging due to the limitations of existing three-dimensional fluorescence micro...

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Veröffentlicht in:	Nature methods 2022-03, Vol.19 (3), p.359-369
Hauptverfasser:	Zhao, Yuxuan, Zhang, Meng, Zhang, Wenting, Zhou, Yao, Chen, Longbiao, Liu, Qing, Wang, Peng, Chen, Rong, Duan, Xinxin, Chen, Feifan, Deng, Huan, Wei, Yunfei, Fei, Peng, Zhang, Yu-Hui
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/245 631/1647/328/2237 631/1647/328/2238 631/80/2373/2238 Algorithms Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology Deep learning Endoplasmic reticulum Endoplasmic Reticulum - metabolism Fluorescence Fluorescence microscopy Image resolution Imaging, Three-Dimensional - methods Intracellular Life Sciences Light sheets Machine learning Microscopy Microscopy, Fluorescence - methods Mitochondria Photobleaching Proteomics Sidelobe reduction Sidelobes Spatial discrimination Spatial resolution
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