Thermo-responsive hydrogels based on methylcellulose/Persian gum loaded with taxifolin enhance bone regeneration: an in vitro/in vivo study
In-situ forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded...
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| Veröffentlicht in: | Cellulose (London) 2022-03, Vol.29 (4), p.2413-2433 |
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| creator | Sajadi-Javan, Zahra Sadat Varshosaz, Jaleh Mirian, Mina Manshaei, Maziar Aminzadeh, Atousa |
| description | In-situ
forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded halloysite nanotubes (HNTs) to enhance mechanical and biological characteristics of the hydrogel in bone tissue engineering. The injectability, mechanical and rheological tests were performed for different hydrogel formulations containing 0.25–1.5 w/v% PG and 1–7 w/v% HNTs. Also, to evaluate the impact of PG and HNTs on hydrogel behavior, the degradation rate and swelling degree of hydrogels were assessed. The optimized MC/PG/HNTs hydrogel containing 1% PG and 3% HNTs (MC/PG-1/HNTs 3%) was easily injectable and gelled rapidly at physiological temperature, while it had the highest mechanical strength due to the existence of PG and HNTs.
In vitro
release study of TAX from this system also revealed more sustained release compared to HNTs-TAX nanoparticles. Furthermore, the interaction of cells with hydrogel and osteo-conductivity was studied using osteoblast-like cells (MG-63). Results showed higher cell adhesion, proliferation, and gene expression for MC/PG-1/HNTs-TAX hydrogel compared to MC/PG-1 and MC/PG-1/HNTs 3% possibly due to the synergic effect of HNTs and TAX. In addition, Alizarin Red S staining and alkaline phosphatase measurements indicated that the existence of HNTs-TAX promoted osteogenic differentiation. Eventually, animal studies on the femoral defects indicated improved remedy when using the MC/PG-1/HNTs-TAX hydrogel carrying MG-63 cells.
Graphical abstract |
| doi_str_mv | 10.1007/s10570-021-04383-8 |
| format | Article |
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forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded halloysite nanotubes (HNTs) to enhance mechanical and biological characteristics of the hydrogel in bone tissue engineering. The injectability, mechanical and rheological tests were performed for different hydrogel formulations containing 0.25–1.5 w/v% PG and 1–7 w/v% HNTs. Also, to evaluate the impact of PG and HNTs on hydrogel behavior, the degradation rate and swelling degree of hydrogels were assessed. The optimized MC/PG/HNTs hydrogel containing 1% PG and 3% HNTs (MC/PG-1/HNTs 3%) was easily injectable and gelled rapidly at physiological temperature, while it had the highest mechanical strength due to the existence of PG and HNTs.
In vitro
release study of TAX from this system also revealed more sustained release compared to HNTs-TAX nanoparticles. Furthermore, the interaction of cells with hydrogel and osteo-conductivity was studied using osteoblast-like cells (MG-63). Results showed higher cell adhesion, proliferation, and gene expression for MC/PG-1/HNTs-TAX hydrogel compared to MC/PG-1 and MC/PG-1/HNTs 3% possibly due to the synergic effect of HNTs and TAX. In addition, Alizarin Red S staining and alkaline phosphatase measurements indicated that the existence of HNTs-TAX promoted osteogenic differentiation. Eventually, animal studies on the femoral defects indicated improved remedy when using the MC/PG-1/HNTs-TAX hydrogel carrying MG-63 cells.
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forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded halloysite nanotubes (HNTs) to enhance mechanical and biological characteristics of the hydrogel in bone tissue engineering. The injectability, mechanical and rheological tests were performed for different hydrogel formulations containing 0.25–1.5 w/v% PG and 1–7 w/v% HNTs. Also, to evaluate the impact of PG and HNTs on hydrogel behavior, the degradation rate and swelling degree of hydrogels were assessed. The optimized MC/PG/HNTs hydrogel containing 1% PG and 3% HNTs (MC/PG-1/HNTs 3%) was easily injectable and gelled rapidly at physiological temperature, while it had the highest mechanical strength due to the existence of PG and HNTs.
In vitro
release study of TAX from this system also revealed more sustained release compared to HNTs-TAX nanoparticles. Furthermore, the interaction of cells with hydrogel and osteo-conductivity was studied using osteoblast-like cells (MG-63). Results showed higher cell adhesion, proliferation, and gene expression for MC/PG-1/HNTs-TAX hydrogel compared to MC/PG-1 and MC/PG-1/HNTs 3% possibly due to the synergic effect of HNTs and TAX. In addition, Alizarin Red S staining and alkaline phosphatase measurements indicated that the existence of HNTs-TAX promoted osteogenic differentiation. Eventually, animal studies on the femoral defects indicated improved remedy when using the MC/PG-1/HNTs-TAX hydrogel carrying MG-63 cells.
Graphical abstract</description><subject>Alizarin</subject><subject>Alkaline phosphatase</subject><subject>Biomedical materials</subject><subject>Bioorganic Chemistry</subject><subject>Cell adhesion</subject><subject>Ceramics</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Defects</subject><subject>Differentiation (biology)</subject><subject>Gene expression</subject><subject>Glass</subject><subject>Hydrogels</subject><subject>In vivo methods and tests</subject><subject>Injectability</subject><subject>Nanoparticles</subject><subject>Natural Materials</subject><subject>Organic Chemistry</subject><subject>Original Research</subject><subject>Physical Chemistry</subject><subject>Polymer Sciences</subject><subject>Regeneration (physiology)</subject><subject>Rheological properties</subject><subject>Sustainable Development</subject><subject>Sustained release</subject><subject>Tissue engineering</subject><issn>0969-0239</issn><issn>1572-882X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kMtKxDAUhoMoOI6-gKuA6zq5tE3iTsQbDOhiBHchaU-nHTrJmLSj8wy-tNER3Lk6B873_wc-hM4puaSEiFmkpBAkI4xmJOeSZ_IATWghWCYlez1EE6JKlc5cHaOTGFeEECUYnaDPRQth7bMAceNd7LaA210d_BL6iK2JUGPv8BqGdtdX0Pdj7yPMniHEzji8HNe496ZO1Hs3tHgwH13j-85hcK1xFWDrHeAAS3AQzNB5d4VTLgHbbgh-9rNsPY7DWO9O0VFj-ghnv3OKXu5uFzcP2fzp_vHmep5VvORDJoXklpNCWkaBFY0SJc15lbMql6xUtqiFtWCAStMoa6AoLcttrTgnxhRNxafoYt-7Cf5thDjolR-DSy81K7kohMqlSBTbU1XwMQZo9CZ0axN2mhL9LV3vpeskXf9I1zKF-D4UE-yWEP6q_0l9AYLviE4</recordid><startdate>20220301</startdate><enddate>20220301</enddate><creator>Sajadi-Javan, Zahra Sadat</creator><creator>Varshosaz, Jaleh</creator><creator>Mirian, Mina</creator><creator>Manshaei, Maziar</creator><creator>Aminzadeh, Atousa</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0001-9333-5798</orcidid></search><sort><creationdate>20220301</creationdate><title>Thermo-responsive hydrogels based on methylcellulose/Persian gum loaded with taxifolin enhance bone regeneration: an in vitro/in vivo study</title><author>Sajadi-Javan, Zahra Sadat ; Varshosaz, Jaleh ; Mirian, Mina ; Manshaei, Maziar ; Aminzadeh, Atousa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-8783b3058b21e25f976143c42c48269b5d7bbeae18af9bae56b24bd9330aa5fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Alizarin</topic><topic>Alkaline phosphatase</topic><topic>Biomedical materials</topic><topic>Bioorganic Chemistry</topic><topic>Cell adhesion</topic><topic>Ceramics</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Defects</topic><topic>Differentiation (biology)</topic><topic>Gene expression</topic><topic>Glass</topic><topic>Hydrogels</topic><topic>In vivo methods and tests</topic><topic>Injectability</topic><topic>Nanoparticles</topic><topic>Natural Materials</topic><topic>Organic Chemistry</topic><topic>Original Research</topic><topic>Physical Chemistry</topic><topic>Polymer Sciences</topic><topic>Regeneration (physiology)</topic><topic>Rheological properties</topic><topic>Sustainable Development</topic><topic>Sustained release</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sajadi-Javan, Zahra Sadat</creatorcontrib><creatorcontrib>Varshosaz, Jaleh</creatorcontrib><creatorcontrib>Mirian, Mina</creatorcontrib><creatorcontrib>Manshaei, Maziar</creatorcontrib><creatorcontrib>Aminzadeh, Atousa</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Materials Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Cellulose (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sajadi-Javan, Zahra Sadat</au><au>Varshosaz, Jaleh</au><au>Mirian, Mina</au><au>Manshaei, Maziar</au><au>Aminzadeh, Atousa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Thermo-responsive hydrogels based on methylcellulose/Persian gum loaded with taxifolin enhance bone regeneration: an in vitro/in vivo study</atitle><jtitle>Cellulose (London)</jtitle><stitle>Cellulose</stitle><date>2022-03-01</date><risdate>2022</risdate><volume>29</volume><issue>4</issue><spage>2413</spage><epage>2433</epage><pages>2413-2433</pages><issn>0969-0239</issn><eissn>1572-882X</eissn><abstract>In-situ
forming hydrogels have gained noticeable attention to encapsulate osteogenic agents and regenerate irregular-shape bone defects. In this study, a novel thermosensitive hydrogel based on blended methylcellulose (MC) with Persian gum (PG) was fabricated and enriched with taxifolin (TAX) loaded halloysite nanotubes (HNTs) to enhance mechanical and biological characteristics of the hydrogel in bone tissue engineering. The injectability, mechanical and rheological tests were performed for different hydrogel formulations containing 0.25–1.5 w/v% PG and 1–7 w/v% HNTs. Also, to evaluate the impact of PG and HNTs on hydrogel behavior, the degradation rate and swelling degree of hydrogels were assessed. The optimized MC/PG/HNTs hydrogel containing 1% PG and 3% HNTs (MC/PG-1/HNTs 3%) was easily injectable and gelled rapidly at physiological temperature, while it had the highest mechanical strength due to the existence of PG and HNTs.
In vitro
release study of TAX from this system also revealed more sustained release compared to HNTs-TAX nanoparticles. Furthermore, the interaction of cells with hydrogel and osteo-conductivity was studied using osteoblast-like cells (MG-63). Results showed higher cell adhesion, proliferation, and gene expression for MC/PG-1/HNTs-TAX hydrogel compared to MC/PG-1 and MC/PG-1/HNTs 3% possibly due to the synergic effect of HNTs and TAX. In addition, Alizarin Red S staining and alkaline phosphatase measurements indicated that the existence of HNTs-TAX promoted osteogenic differentiation. Eventually, animal studies on the femoral defects indicated improved remedy when using the MC/PG-1/HNTs-TAX hydrogel carrying MG-63 cells.
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| ispartof | Cellulose (London), 2022-03, Vol.29 (4), p.2413-2433 |
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| subjects | Alizarin Alkaline phosphatase Biomedical materials Bioorganic Chemistry Cell adhesion Ceramics Chemistry Chemistry and Materials Science Composites Defects Differentiation (biology) Gene expression Glass Hydrogels In vivo methods and tests Injectability Nanoparticles Natural Materials Organic Chemistry Original Research Physical Chemistry Polymer Sciences Regeneration (physiology) Rheological properties Sustainable Development Sustained release Tissue engineering |
| title | Thermo-responsive hydrogels based on methylcellulose/Persian gum loaded with taxifolin enhance bone regeneration: an in vitro/in vivo study |
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